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- Kiziak, Christoph, et al. Show all 2 Author
- Applied and environmental microbiology 2009 v.75 no.17 pp. 5592-5599
- Alcaligenes faecalis; Pseudomonas fluorescens; acids; alanine; enantiomers; enzymes; histidine; phenylalanine; point mutation; tryptophan; tyrosine
- ... The nitrilase from Pseudomonas fluorescens EBC191 converted (R,S)-mandelonitrile with a low enantioselectivity to (R)-mandelic acid and (S)-mandeloamide in a ratio of about 4:1. In contrast, the same substrate was hydrolyzed by the homologous nitrilase from Alcaligenes faecalis ATCC 8750 almost exclusively to (R)-mandelic acid. A chimeric enzyme between both nitrilases was constructed, which repre ...
- Kiziak, Christoph, et al. Show all 6 Authors
- Applied and environmental microbiology 2010 v.76 no.11 pp. 3668-3674
- Escherichia coli; Pseudomonas fluorescens; alanine; amides; asparagine; benzaldehyde; cyanides; cysteine; enzymes; glutamine; mandelic acid; mutants; mutation; neighborhoods; plasmids; serine
- ... The arylacetonitrilase from Pseudomonas fluorescens EBC191 differs from previously studied arylacetonitrilases by its low enantiospecificity during the turnover of mandelonitrile and by the large amounts of amides that are formed in the course of this reaction. In the sequence of the nitrilase from P. fluorescens, a cysteine residue (Cys163) is present in direct neighborhood (toward the amino term ...