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- Korde, A., et al. Show all 8 Authors
- Journal of agricultural and food chemistry 2003 v.51 no.4 pp. 843-846
- aflatoxins; radioimmunoassays; polyethylene glycol; separation; radionuclides; iodine; isotope labeling; food contamination; peanuts; corn; soybeans; wheat; rice; smokeless tobacco
- ... A radioimmunoassay (RIA) procedure to measure aflatoxin B1 (AfB1) in agricultural commodities was developed. AfB1 oxime derivative was synthesized, characterized, and used for preparation of 125I-labeled AfB1. Antiaflatoxin B1 serum was raised in-house using AfB1-bovine serum albumin conjugate as immunogen. The assay system was optimized in the range of 0.2-5 ng/mL, using a liquid phase (PEG) as w ...