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- Lee, Eon-Seok, et al. Show all 7 Authors
- Biotechnology and bioprocess engineering 2013 v.18 no.2 pp. 375-381
- Escherichia coli; Pyrococcus furiosus; bacteria; calcium chloride; gene expression; genes; glutamate decarboxylase; glutamic acid; half life; heat inactivation; molecular weight; pH; polyacrylamide gel electrophoresis; potassium chloride; sodium chloride; temperature; thermal stability
- ... Gene encoding for a putative glutamate decarboxylase (GAD: EC 220.127.116.11) from the hyperthermophilic archaeon Pyrococcus furiosus was cloned and the biochemical characteristics of the resulting recombinant protein were examined. The gene (PF1159) from P. furiosus showed some identity with other group II decarboxylases from an archaea and bacteria. The GAD from P. furiosus (PfGAD) was expressed in Es ...