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1. Redox reaction-modulated fluorescence biosensor for ascorbic acid oxidase assay by using MoS2 quantum dots as fluorescence probe

Author:
Li, Ning,   et al.   ; Zhang, Feng; Sun, Wenying; Zhang, Lili; Su, Xingguang;     Show all 5 Authors
Source:
Talanta 2021 v.222 pp. 121522
ISSN:
0039-9140
Subject:
ascorbate oxidase; ascorbic acid; biosensors; blood serum; cobalt; dehydroascorbic acid; energy transfer; fluorescence; fluorescent dyes; humans; molybdenum disulfide; oxidation; quantum dots
Abstract:
... Herein, a sensitive fluorescence (FL) biosensor for the assay of ascorbic acid oxidase (AAO) was established based on the fluorescence resonance energy transfer (FRET) between MoS₂ quantum dots (MQDs) and CoOOH nanoflakes. CoOOH nanoflakes as effective FL quencher could quench the FL signal of MQDs on the basis of FRET. When ascorbic acid (AA) was added to the MQDs/CoOOH nanoflakes system, the FL ...
DOI:
10.1016/j.talanta.2020.121522

2. Two new species of Panorpa (Mecoptera, Panorpidae) from the Hengduan Mountains in Yunnan, China

Author:
Li, Ning,   et al.   ; Hua, Bao-Zhen;     Show all 2 Author
Source:
Journal of Asia-Pacific entomology 2020 v.23 no.1 pp. 138-145
ISSN:
1226-8615
Subject:
Panorpa; females; insect taxonomy; male genitalia; mountains; new species; taxonomic keys; wings; China
Abstract:
... Two new species of the genus Panorpa Linnaeus, 1758 are described from the Hengduan Mountains in Yunnan, China. Panorpa diqingensissp. n. can be distinguished from its congeners by vertex dark brown, and occiput yellow; pleura dark brown; gonocoxites bearing a cluster of stout black setae on inner apex; parameres S-shaped in male genitalia. Panorpa dispergenssp. n. can be readily differentiated fr ...
DOI:
10.1016/j.aspen.2019.12.005

3. Numerical wave modeling for operational and survival analyses of wave energy converters at the US Navy Wave Energy Test Site in Hawaii

Author:
Li, Ning,   et al.   ; Cheung, Kwok Fai; Cross, Patrick;     Show all 3 Authors
Source:
Renewable energy 2020 v.161 pp. 240-256
ISSN:
0960-1481
Subject:
climate models; data collection; energy conversion; hurricanes; statistical analysis; trade winds; water power; water waves; Oahu
Abstract:
... The US Navy Wave Energy Test Site (WETS) is currently operating three grid-connected berths off Marine Corps Base Hawaii in support of technological development through scaled-model testing and pre-commercial prototyping. The location on the east shore of Oahu is open to year-round wind waves and seasonal north swells as well as severe seas from subtropical systems and hurricanes. We have assemble ...
DOI:
10.1016/j.renene.2020.06.089

4. Population Spatialization in Beijing City Based on Machine Learning and Multisource Remote Sensing Data

Author:
Li, Ning,   et al.   ; He, Miao; Xu, Yongming;     Show all 3 Authors
Source:
Remote Sensing 2020 v.12 no.12
ISSN:
2072-4292
Subject:
Internet; algorithms; artificial intelligence; census data; cities; disaster preparedness; image analysis; models; population density; public health; remote sensing; spatial data; statistical analysis; uncertainty; urban planning; China
Abstract:
... Remote sensing data have been widely used in research on population spatialization. Previous studies have generally divided study areas into several sub-areas with similar features by artificial or clustering algorithms and then developed models for these sub-areas separately using statistical methods. These approaches have drawbacks due to their subjectivity and uncertainty. In this paper, we pre ...
DOI:
10.3390/rs12121910

5. Flexural behavior of bamboo–concrete composite beams with perforated steel plate connections

Author:
Li, Ning,   et al.   ; Wang, Zhiyuan; Wei, Yang; Zhao, Kang; Ding, Mingmin;     Show all 5 Authors
Source:
Journal of wood science 2020 v.66 no.1 pp. 4
ISSN:
1435-0211
Subject:
bamboos; concrete; steel
Abstract:
... A new type of bamboo–concrete composite structure using perforated steel plates as connectors was proposed. To study the composite effect of this new type of composite structure, the slip behavior of bamboo–concrete shear connectors was first studied through push-out tests. Subsequently, four-point bending tests of ten bamboo–concrete composite beams were carried out. The results show that the fai ...
DOI:
10.1186/s10086-020-1854-9

6. Importance of plant and bird traits on the seed removal pattern of endangered trees across different forest patches in southeast China

Author:
Li, Ning,   et al.   ; Zhang, Shuai; Ren, Yuan-Hao; Wang, Zheng;     Show all 4 Authors
Source:
Ecological processes 2020 v.9 no.1 pp. 43
ISSN:
2192-1709
Subject:
Taxus wallichiana var. chinensis; birds; body weight; canopy; endangered species; forage; foraging; forests; fruits; gardens; seed dispersal; seeds; tree height; trees; China
Abstract:
... Seed removal by birds has been recognized as an important part of seed dispersal process in patchy forest areas; however, few studies have focused on the contribution of both plant and bird traits to seed removal. In this study, field data of seed removal for Taxus chinensis was combined with the data of bird and plant traits for analysis. The relevant traits of plants and birds that influenced T. ...
DOI:
10.1186/s13717-020-00253-6

7. High sensitive ratiometric fluorescence analysis of trypsin and dithiothreitol based on WS2 QDs

Author:
Li, Ning,   et al.   ; Duan, Xinhe; Wang, Guannan; Su, Xingguang;     Show all 4 Authors
Source:
Talanta 2020 v.219 pp. 121171
ISSN:
0039-9140
Subject:
blood serum; cytochrome c; detection limit; disulfides; dithiothreitol; energy transfer; enzyme activity; fluorescence; humans; hydrogen peroxide; hydroxyl radicals; oxidation; quantum dots; trypsin; tungsten
Abstract:
... In this paper, a simple tungsten disulfide quantum dots (WS₂ QDs)-based ratiometric fluorescence method was established for the detection of trypsin and 1, 4-dithiothreitol. Trypsin can hydrolyze cytochrome c into heme-peptide fragments with peroxidase-like activity. In the presence of hydrogen peroxide, the fragments can generates hydroxyl radicals, which can oxidize o-phenylenediamine (OPD) to f ...
DOI:
10.1016/j.talanta.2020.121171

8. Eliminating sweet spot in MALDI-MS with hydrophobic ordered structure as target for quantifying biomolecules

Author:
Li, Ning,   et al.   ; Dou, Shuzhen; Feng, Lei; Zhu, Qunyan; Lu, Nan;     Show all 5 Authors
Source:
Talanta 2020 v.218 pp. 121172
ISSN:
0039-9140
Subject:
angiotensin III; bacitracin; biosensors; heart; hydrophobicity; matrix-assisted laser desorption-ionization mass spectrometry; milk; myoglobin; nanotechnology; polymethylmethacrylate; salt concentration; signal-to-noise ratio; silicon
Abstract:
... In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), the analyte is usually distributed unevenly throughout the sample spot. The area with aggregated analyte molecules contributing abundant signal, is termed as “sweet spot”, which results in poor detection reproducibility and makes it impossible to quantify analytes without internal standards. We proposed a strategy to elim ...
DOI:
10.1016/j.talanta.2020.121172

9. Chemical constituents with cytotoxic and anti-inflammatory activity in Hypericum sampsonii and the antitumor potential under the view of cancer-related inflammation

Author:
Li, Ning,   et al.   ; Chen, Qian; Di, Lei; Zhang, Yi;     Show all 4 Authors
Source:
Journal of ethnopharmacology 2020 v.259 pp. 112948
ISSN:
0378-8741
Subject:
Hypericum sampsonii; alkaloids; anti-inflammatory activity; antineoplastic activity; arthritis; benzophenones; bone marrow; breast neoplasms; cell lines; chemical composition; colorectal neoplasms; cytotoxicity; dermatitis; ethanol; flavonoids; high performance liquid chromatography; human diseases; humans; inflammation; inhibitory concentration 50; medicinal plants; melanoma; neoplasm cells; nitric oxide; phenols; screening; spectroscopy; sterols; stomach neoplasms; traditional medicine; xanthones; China
Abstract:
... Chronic inflammation has an important role in the development of cancers. Hypericum sampsonii, known as “Yuanbao Cao”, is mainly distributed in the southwest of China. As a folk medicinal plant, “Yuanbao Cao” is traditionally used for treatment of various inflammation-related diseases including swelling, burns, arthritis, and dermatitis, etc. The plant is a promising anticancer herb. However, ther ...
DOI:
10.1016/j.jep.2020.112948

10. Transcriptomic analysis of Ruditapes philippinarum under aerial exposure and reimmersion reveals genes involved in stress response and recovery capacity of the Manila clam

Author:
Li, Ning,   et al.   ; Nie, Hongtao; Jiang, Kunyin; Li, Dongdong; Yan, Xiwu;     Show all 5 Authors
Source:
Aquaculture 2020 v.524 pp. 735271
ISSN:
0044-8486
Subject:
Ruditapes philippinarum; Toll-like receptor 6; air; apoptosis; dynein ATPase; energy metabolism; focal adhesions; gene expression; gene expression regulation; genes; immune response; immunosuppression; messenger RNA; resorption; signal transduction; stress response; transcriptomics
Abstract:
... The Manila clam, Ruditapes philippinarum, is an economically and scientifically important bivalve mollusk. Its tolerance of aerial exposure has long been considered an important trait for its survival under acute environmental stress. In this study, the transcriptome sequencing of Manila clam under air exposure and reimmersion stresses were conducted, and the differential expression of stress resp ...
DOI:
10.1016/j.aquaculture.2020.735271

11. Micron-sized silica particles in wastewater influenced the distribution of organic matters in sludge and their anaerobic degradation

Author:
Li, Ning,   et al.   ; Chen, Sisi; Dong, Bin; Yang, Dianhai; Dai, Xiaohu;     Show all 5 Authors
Source:
Journal of hazardous materials 2020 v.393 pp. 122340
ISSN:
0304-3894
Subject:
methane production; polysaccharides; protein content; protein degradation; protein secondary structure; sewage sludge; silica; wastewater; China
Abstract:
... The influence of micron-sized silica particles on the distribution of organic matters in sludge and anaerobic degradation of macromolecular organic components (MOCs) in sludge was investigated. With the addition of micron-sized particles in the influent (VS/TS decreased gradually from 90.46 ± 0.21 % to 33.36 ± 0.17 %), the protein degradation percentage was significantly promoted while the polysac ...
DOI:
10.1016/j.jhazmat.2020.122340

12. Direct chemical vapor deposition of graphene on plasma-etched quartz glass combined with Pt nanoparticles as an independent transparent electrode for non-enzymatic sensing of hydrogen peroxide

Author:
Li, Ning,   et al.   ; Yuan, Yawen; Liu, Jinglei; Hou, Shifeng;     Show all 4 Authors
Source:
RSC advances 2020 v.10 no.35 pp. 20438-20444
ISSN:
2046-2069
Subject:
active sites; carbon tetrafluoride; catalytic activity; detection limit; dispersibility; electrodes; glass; graphene; hydrogen peroxide; nanoparticles; platinum; pollution; quartz; vapors
Abstract:
... In this work, chemical vapor deposition (CVD) method-grown graphene on plasma-etched quartz glass supported platinum nanoparticles (PtNPs/eQG) was constructed as an independent transparent electrode for non-enzymatic hydrogen peroxide (H₂O₂) detection. Graphene grown on quartz glass by the CVD method can effectively reduce the wrinkles and pollution caused by traditional transfer methods. The addi ...
DOI:
10.1039/d0ra01963a

13. Sacrificial Substrate-Free Whole-Cell Biocatalysis for the Synthesis of 2,5-Furandicarboxylic Acid by Engineered Escherichia coli

Author:
Li, Ning,   et al.   ; Wang, Xin; Zhang, Xue-Ying; Zong, Min-Hua;     Show all 4 Authors
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.11 pp. 4341-4345
ISSN:
2168-0485
Subject:
Escherichia coli; aldehyde oxidoreductases; biocatalysis; biocatalysts; dicarboxylic acids; furfural; hydroxymethylfurfural; oxidation; polymers; vanillin
Abstract:
... 2,5-Furandicarboxylic acid (FDCA) is a promising biobased building block for the synthesis of polymers. In this work, a novel whole-cell biocatalyst was constructed by coexpressing vanillin dehydrogenase (VDH1) and HMF/furfural oxidoreductase (HmfH) in Escherichia coli for cascade catalytic oxidation of 5-hydroxymethylfurfural (HMF) to FDCA under sacrificial substrate-free conditions. HMF was rapi ...
DOI:
10.1021/acssuschemeng.0c00058

14. Pyrolysis behavior of xylan-based hemicellulose in a fixed bed reactor

Author:
Li, Ning,   et al.   ; Gao, Zixiang; Wang, Yiqing; Niu, Weisheng; Yi, Weiming;     Show all 5 Authors
Source:
Journal of analytical and applied pyrolysis 2020 v.146 pp. 104772
ISSN:
0165-2370
Subject:
acetaldehyde; acetic acid; arabinose; biofuels; biomass; bubbles; furfural; glucose; heat; industrialization; mass transfer; melting; molecular weight; pentoses; pyrolysis; temperature; xylan; xylose
Abstract:
... Investigation on the biomass components pyrolysis behaviors in a lab-scale reactor is a necessary step before its industrialization due to the poor transportability of the findings obtained from the micro-scale pyrolyzer which often neglects the heat and mass transfer effect. In present study, the pyrolysis behavior of xylan-based hemicellulose in a fixed bed reactor was investigated. The residue ...
DOI:
10.1016/j.jaap.2020.104772

15. Understanding the interdependence of strain of electrotroph, cathode potential and initial Cu(II) concentration for simultaneous Cu(II) removal and acetate production in microbial electrosynthesis systems

Author:
Li, Ning,   et al.   ; Hou, Jiaxin; Huang, Liping; Zhou, Peng; Qian, Yitong;     Show all 5 Authors
Source:
Chemosphere 2020 v.243 pp. 125317
ISSN:
0045-6535
Subject:
Citrobacter; Pseudomonas aeruginosa; Stenotrophomonas maltophilia; acetates; biofilm; cathodes; copper; electrosynthesis; heavy metals; hydrogen; plankton; polymers; value-added products
Abstract:
... Metallurgical microbial electrosynthesis systems (MES) are holding great promise for simultaneous heavy metal removal and acetate production from heavy metal-contaminated and organics-barren waters. How critical parameters of strain of electrotroph, cathode potential and initial heavy metal concentration affect MES performance, however, is not yet fully understood. Heavy metal of Cu(II) and four C ...
DOI:
10.1016/j.chemosphere.2019.125317

16. The tumor suppressive effect of long non-coding RNA FRMD6-AS2 in uteri corpus endometrial carcinoma

Author:
Li, Ning,   et al.   ; Wang, Jing; Li, Zenghui; Wang, Xiuli; Ding, Yan;     Show all 5 Authors
Source:
Life sciences 2020 v.243 pp. 117254
ISSN:
0024-3205
Subject:
carcinoma; cell movement; chromatin; endometrium; gene ontology; genes; morbidity; mortality; non-coding RNA; promoter regions; signal transduction; uterine neoplasms
Abstract:
... Uterine corpus endometrial cancer (UCEC) is one of the most common gynecological malignancies with increasing incidence and high morbidity and mortality. The currently acknowledged molecular mechanism of UCEC is still not adequate. Here, we reported that the expression of a novel long non-coding RNA (lncRNA) FRMD6-AS2 was reduced in UCEC compared to noncancerous endometrium tissues using the data ...
DOI:
10.1016/j.lfs.2020.117254

17. Making a cup of carbon dots for ratiometric and colorimetric fluorescent detection of Cu2+ ions

Author:
Li, Ning,   et al.   ; Zhang, Wenjing; Chang, Qing; Chen, Zhenfei; Hu, Shengliang;     Show all 5 Authors
Source:
Colloids and surfaces 2020 v.586 pp. 124233
ISSN:
0927-7757
Subject:
brewing; carbon quantum dots; colorimetry; copper; energy transfer; fluorescence; heat treatment; ions; iron; moieties; oxidation; tea
Abstract:
... Fluorescence carbon dots (CDs) are firstly obtained from microbial fermented Pu-erh tea by a simple brewing method without high temperature treatment as reported in literature. The obtained CDs contain abundant surface functional groups and exhibit a good ability to detect Fe and Cu ions. Moreover, a new sensing universal platform that can simultaneously apply fluorescent and colorimetric dual-rea ...
DOI:
10.1016/j.colsurfa.2019.124233

18. Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment

Author:
Li, Ning,   et al.   ; Chen, I-Hsuan; Xiao, Hui; Daly, Thomas;     Show all 4 Authors
Source:
Analytical chemistry 2020 v.92 no.5 pp. 3751-3757
ISSN:
1520-6882
Subject:
biopharmaceuticals; detergents; enzyme-linked immunosorbent assay; filtration; liquid chromatography; mass spectrometry; molecular weight; monitoring; monoclonal antibodies; proteins; proteomics; purification methods; therapeutics
Abstract:
... Host cell proteins (HCPs) are process-related impurities that are generated by the host organism and are typically present at low levels in recombinant biopharmaceutical products, such as therapeutic antibodies. While overall HCP levels are usually monitored by enzyme-linked immunosorbent assay (ELISA), liquid chromatography coupled to mass spectrometry (LC-MS) is emerging as a powerful tool that ...
DOI:
10.1021/acs.analchem.9b05081

19. Establishment and characterization of immortalized porcine neonatal hepatocytes without the use of viral components

Author:
Li, Ning,   et al.   ; Zou, Yunlong; Hao, Haiyang; Li, Qiuyan;     Show all 4 Authors
Source:
In vitro cellular & developmental biology 2020 v.56 no.1 pp. 75-84
ISSN:
1071-2690
Subject:
Western blotting; electroporation; gene expression; glycogen; hepatocytes; lipids; liver; liver diseases; oncogenes; pathogenesis; reverse transcriptase polymerase chain reaction; risk; secretion; serum albumin; swine; transfection; xenotransplantation
Abstract:
... Porcine hepatocytes are a promising option for xenotransplantation in light of the critical shortage of orthotopic donor livers. Because primary hepatocytes have limited ability to proliferate in vitro, several immortalized hepatocyte lines have been established. However, these cells have typically been generated using a virus-dependent transfection methodology and express viral oncogenes that int ...
DOI:
10.1007/s11626-019-00407-7

20. Assessment of the economic cascading effect on future climate change in China: Evidence from agricultural direct damage

Author:
Li, Ning,   et al.   ; Huang, Chengfang; Zhang, Zhengtao; Liu, Yuan; Chen, Xi; Wang, Fang;     Show all 6 Authors
Source:
Journal of cleaner production 2020 v.276 pp. 123951
ISSN:
0959-6526
Subject:
agricultural industry; agricultural land; climate change; economic impact; economic systems; manufacturing; models; temperature; China
Abstract:
... The agricultural sector is extremely vulnerable to climate change and also plays a very important role in the operation of the entire economic system, leading many studies to evaluate the possible agricultural direct economic damage (ADED) of climate change. However, this damage may have an amplification impact through the intricate industrial linkages that exist among sectors, and this impact may ...
DOI:
10.1016/j.jclepro.2020.123951