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1. Pattern of Specific Oxidation of Konjac Glucomannan with TEMPO/NaBr/NaClO system

Author:
Lin Yi,   et al.   ; Fangxia Wu; Na Yan; Yan Guo; Xiaoxiao Yu; Yilan Ouyang; Xin Wang; Zhenqing Zhang;     Show all 8 Authors
Source:
Carbohydrate research 2022 v.515 pp. 108558
ISSN:
0008-6215
Subject:
glucose; hydrogen bonding; konjac mannan; mannose; molecular weight; oligosaccharides; oxidants; oxidation; oxygen; research; sodium bromide
Abstract:
... The TEMPO/NaBr/NaClO system was used to modify konjac glucomannan and prepare β-1,4-linked D-mannuronic/glucuronic acid oligosaccharides. The impact of oxidant amount on the degree of oxidation, molecular weight, terminal structure and the ratio of sugar units were systematically investigated. Mannoses were transformed to mannuronic acids quantitatively, while some glucose were transformed to gluc ...
DOI:
10.1016/j.carres.2022.108558

2. Sequencing analysis of heparin reducing terminals with orthogonal chromatographic approaches

Author:
Lin Yi,   et al.   ; Wen Zhu; Lei Chen; Na Yan; Yuanyuan Sun; Yilan Ouyang; Dehua Liu; Zhenqing Zhang;     Show all 8 Authors
Source:
Journal of chromatography 2022 v.1677 pp. 463318
ISSN:
0021-9673
Subject:
biosynthesis; chromatography; digestion; heparin; heparin lyase; transferases
Abstract:
... Heparin is a linear sulfated polysaccharide with a complex structure. It is important to figure out the sequences at the terminals of the sugar chains, as it will help us understand the heparin structure deeper and control its quality properly. The tetrasaccharide linkage region (LR) could be a tag to help us find out heparin terminals after digestion by different combinations of heparinases. In t ...
DOI:
10.1016/j.chroma.2022.463318

3. Glycan Mapping of Low-Molecular-Weight Heparin Using Mass Spectral Correction Based on Chromatography Fitting with “Glycomapping” Software

Author:
Lin Yi,   et al.   ; Na Yan; Feifan Song; Yilan Ouyang; Duxin Li; He Tian; Robert J. Linhardt; Zhenqing Zhang;     Show all 8 Authors
Source:
Analytical chemistry 2022 v.94 no.38 pp. 13000-13009
ISSN:
1520-6882
Subject:
analytical chemistry; animals; computer software; databases; enoxaparin; gel chromatography; mass spectrometry
Abstract:
... In this work, the first version of “Glycomapping” software is developed for the analysis of the most common low-molecular-weight heparin (LMWH), enoxaparin. Using ultrahigh-performance liquid chromatography-mass spectrometry, size exclusion chromatography is applied, and a virtual database of glycans in enoxaparin is established for the initial searching. With “Glycomapping”, a complex chromatogra ...
DOI:
10.1021/acs.analchem.2c01579

4. Construction of PEGylated boronate-affinity-oriented imprinting magnetic nanoparticles for ultrasensitive detection of ellagic acid from beverages

Author:
Lin-Yi Dong,   et al.   ; Meng-Ying Chen; Jin-Ye Lang; Chen-Chen Bai; Shi-Song Yu; Xiang-Jin Kong; Xian-Hua Wang;     Show all 7 Authors
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.22 pp. 6557-6570
ISSN:
1618-2642
Subject:
adsorption; beverages; chemical bonding; detection limit; ellagic acid; fluorescence; graphene oxide; hydrophilicity; magnetism; molecular imprinting; nanoparticles; standard deviation
Abstract:
... Molecularly imprinted polymers (MIPs) can exhibit antibody-level affinity for target molecules. However, the nonspecific adsorption of non-imprinted regions for non-target molecules limits the application range of MIPs. Herein, we fabricated PEGylated boronate-affinity-oriented ellagic acid-imprinting magnetic nanoparticles (PBEMN), which first integrated boronate-affinity-oriented surface imprint ...
DOI:
10.1007/s00216-022-04213-1

5. Poly(caffeic acid)-coated molecularly imprinted magnetic nanoparticles for specific and ultrasensitive detection of glycoprotein

Author:
Lin-Yi Dong,   et al.   ; Zhi-Heng Pan; Shi-Song Yu; Chen-Chen Bai; Wen-Yu Yin; Yi-Rong Ma; Zi-Ang Xue; Qin-Yue Lu; Xian-Hua Wang;     Show all 9 Authors
Source:
Talanta 2022 v.241 pp. 123240
ISSN:
0039-9140
Subject:
1,6-hexanediamine; autoxidation; blood serum; caffeic acid; detection limit; glycoproteins; humans; hydrophilicity; magnetism; molecular imprinting; polyphenols; standard deviation; transferrin
Abstract:
... Molecularly imprinted polymers (MIPs) are artificial chemical receptors, and can recognize template molecules with a high selectivity and affinity. As “antibody mimics”, MIPs have been widely studied in various fields. However, the general applicability of MIPs is limited by the type of functional monomers. Herein, we developed caffeic acid (CA, a natural polyphenol) as novel a functional monomer. ...
DOI:
10.1016/j.talanta.2022.123240

6. The Advances of Broad-Spectrum and Hot Anti-Coronavirus Drugs

Author:
Lin Yi,   et al.   ; Sen Zeng; Yuwan Li; Wenhui Zhu; Zipeng Luo; Keke Wu; Xiaowen Li; Yiqi Fang; Yuwei Qin; Wenxian Chen; Zhaoyao Li; Linke Zou; Xiaodi Liu; Shuangqi Fan;     Show all 14 Authors
Source:
Microorganisms 2022 v.10 no.7 pp. -
ISSN:
2076-2607
Subject:
Betacoronavirus 1; Infectious bronchitis virus; Middle East respiratory syndrome coronavirus; Murine hepatitis virus; Porcine epidemic diarrhea virus; Severe acute respiratory syndrome coronavirus 2; antiviral agents; chickens; diarrhea; drug therapy; gastroenteritis; humans; immunization; morbidity; mortality; public health; swine; vaccines; viruses
Abstract:
... Coronaviruses, mainly including severe acute respiratory syndrome virus, severe acute respiratory syndrome coronavirus 2, Middle East respiratory syndrome virus, human coronavirus OC43, chicken infectious bronchitis virus, porcine infectious gastroenteritis virus, porcine epidemic diarrhea virus, and murine hepatitis virus, can cause severe diseases in humans and livestock. The severe acute respir ...
DOI:
10.3390/microorganisms10071294

7. Structural elucidation of a branch-on-branch β-glucan from Hericium erinaceus with A HPAEC-PAD-MS system

Author:
Lin Yi,   et al.   ; Bingying Xie; Yiting Zhu; Xiaoming Chang; Jie Hao; Li Pang; Yilan Ouyang; Sheng Yuan; Zhenqing Zhang;     Show all 9 Authors
Source:
Carbohydrate polymers 2021 v.251 pp. 117080
ISSN:
0144-8617
Subject:
Hericium; digestion; enzymes; fractionation; glucose; glycosidic linkages; molecular weight; oligosaccharides; water solubility
Abstract:
... A water-soluble β-glucan from fruiting body of Hericium erinaceus was obtained after water extraction, purification and fractionation. Analyses of monosaccharide composition, molecular weight and linkage mode demonstrated that it is a β-glucan with 1→3 and 1→6 linkage modes and a molecular weight of 13.3 kDa. An endo-1,6-β-d-glucanase was used to digest the β-glucan and the digested products over ...
DOI:
10.1016/j.carbpol.2020.117080

8. Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of avian orthoavulavirus 1

Author:
Lin Yi,   et al.   ; Jindai Fan; Wenxian Chen; Yuanyuan Zhang; Zhixiang Liu; Xiaoming Li; Hongxing Ding; Jinding Chen; Mingqiu Zhao;     Show all 9 Authors
Source:
Journal of veterinary diagnostic investigation 2021 v.33 no.2 pp. 308-312
ISSN:
1943-4936
Subject:
Avian orthoavulavirus 1; Newcastle disease; birds; cross reaction; detection limit; poultry industry; rapid methods; recombinase polymerase amplification; reverse transcription
Abstract:
... Newcastle disease is an avian infectious disease caused by avian orthoavulavirus 1, also known as Newcastle disease virus (NDV). This disease has caused significant economic losses to the poultry industry worldwide. The rapid and simple detection of NDV infection is crucial to inform the appropriate control measures. We developed a reverse-transcription recombinase polymerase amplification (RT-RPA ...
DOI:
10.1177/1040638721990122

9. Assessment of ultra-lean burn characteristics for a stratified-charge direct-injection spark-ignition methanol engine under different high compression ratios

Author:
Lin Yi,   et al.   ; Changming Gong; Zilei Zhang; Jingzhen Sun; Fenghua Liu;     Show all 5 Authors
Source:
Applied energy 2020 v.261 pp. 114478
ISSN:
0306-2619
Subject:
air; carbon monoxide; combustion; emissions; methanol; nitric oxide; soot; spark ignition engines
Abstract:
... Lean burn is one of the most important characteristics of a stratified-charge direct-injection spark-ignition engine. In this study, a non-uniform 10-hole × 0.30 mm spray-line distribution nozzle was chosen to achieve stratified-charge of the methanol/air mixture. The mixture formation, combustion, and emissions characteristics of a stratified-charge direct-injection spark-ignition methanol engine ...
DOI:
10.1016/j.apenergy.2019.114478

10. Numerical study on electron energy distribution characteristics and evolution of active particles of methanol-air mixture by non-equilibrium plasma

Author:
Lin Yi,   et al.   ; Changming Gong; Kang Wang; Kuo Huang; Fenghua Liu;     Show all 5 Authors
Source:
Energy 2020 v.193 pp. 116881
ISSN:
0360-5442
Subject:
combustion; economics; electric field; free radicals; ionization
Abstract:
... The extent of combustion enhancement by non-equilibrium plasma strongly depends on the electric field and electron number density. The electron energy distribution characteristics and evolution of active particles of methanol-air mixture by non-equilibrium plasma were numerical simulated. As an introductory work in this field, this study only partially cited other people’s data for comparative ver ...
DOI:
10.1016/j.energy.2019.116881

11. Numerical modeling of plasma-assisted combustion effects on firing and intermediates in the combustion process of methanol–air mixtures

Author:
Lin Yi,   et al.   ; Changming Gong; Kang Wang; Kuo Huang; Fenghua Liu;     Show all 5 Authors
Source:
Energy 2020 v.192 pp. 116598
ISSN:
0360-5442
Subject:
carbon dioxide; carbon monoxide; combustion; fuels; mathematical models; methanol; oxygen; reaction kinetics; temperature
Abstract:
... The effects of plasma-assisted ignition and combustion on the ignition-delay time (Tᵢd) and intermediates in methanol combustion were simulated numerically through a chemical-kinetics mechanism of methanol oxidation. Plasma-assisted ignition can considerably shorten the Tᵢd. Plasma-assisted ignition is more conducive to a low temperature and the lean combustion of a methanol-air mixture. According ...
DOI:
10.1016/j.energy.2019.116598

12. Evaluation of the heterogeneity in the intensity of human interference on urbanized coastal ecosystems: Shenzhen (China) as a case study

Author:
Lin Yi,   et al.   ; Zhaoyuan Yu; Jing Qian; Muhammadjon Kobuliev; Chaoliang Chen; Xiuwei Xing;     Show all 6 Authors
Source:
Ecological indicators 2021 v.122 pp. 107243
ISSN:
1470-160X
Subject:
anthropogenic activities; case studies; coasts; humans; landscapes; quantitative analysis; urbanization; China
Abstract:
... Quantitative measurement of the heterogeneity in the intensity of human interference is key to accurately assessing the impact of human activity. The same human activities in different landscape configurations should have different impacts. This study constructed a weighted analysis to describe and measure heterogeneity under the Hemeroby index model framework, based on gradient structures analysi ...
DOI:
10.1016/j.ecolind.2020.107243

13. Viral Vector Vaccine Development and Application during the COVID-19 Pandemic

Author:
Lin Yi,   et al.   ; Shaofeng Deng; Hui Liang; Pin Chen; Yuwan Li; Zhaoyao Li; Shuangqi Fan; Keke Wu; Xiaowen Li; Wenxian Chen; Yuwei Qin; Jinding Chen;     Show all 12 Authors
Source:
Microorganisms 2022 v.10 no.7 pp. -
ISSN:
2076-2607
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; genetic engineering; pandemic; respiratory system; vaccination; vaccine development; vector vaccines
Abstract:
... With the accumulation of mutations in SARS-CoV-2 and the continuous emergence of new variants, the importance of developing safer and effective vaccines has become more prominent in combating the COVID-19 pandemic. Both traditional and genetically engineered vaccines have contributed to the prevention and control of the pandemic. However, in recent years, the trend of vaccination research has grad ...
DOI:
10.3390/microorganisms10071450

14. High starch accumulation mechanism and phosphorus utilization efficiency of duckweed (Landoltia punctata) under phosphate starvation

Author:
Zhuo-lin Yi,   et al.   ; Jin-meng Li; An-ping Du; Peng-hui Liu; Xue-ping Tian; Yan-ling Jin; Kai-ze He; Yang Fang; Hai Zhao;     Show all 9 Authors
Source:
Industrial crops and products 2021 v.167 pp. 113529
ISSN:
0926-6690
Subject:
Araceae; Landoltia punctata; biochemical pathways; biomass production; carbon; glucose-1-phosphate adenylyltransferase; phosphates; phosphorus; plant growth; reproduction; starch; starch synthase; starvation; total phosphorus; transcriptomics; vacuoles
Abstract:
... Phosphorus is an essential element for plant growth and reproduction. This study aimed to investigate the metabolic response, growth, and starch accumulation mechanisms in duckweed strain, Landoltia punctata 0202, under phosphate starvation. The results revealed that, under phosphate starvation, the total phosphorus in L. punctata 0202 decreased, while total carbon increased. After 15 days of phos ...
DOI:
10.1016/j.indcrop.2021.113529

15. Anti-Classical Swine Fever Virus Strategies

Author:
Lin Yi,   et al.   ; Jindai Fan; Yingxin Liao; Mengru Zhang; Chenchen Liu; Zhaoyao Li; Yuwan Li; Xiaowen Li; Keke Wu; Hongxing Ding; Mingqiu Zhao; Shuangqi Fan; Jinding Chen;     Show all 13 Authors
Source:
Microorganisms 2021 v.9 no.4 pp. -
ISSN:
2076-2607
Subject:
Classical swine fever virus; Dengue virus; Hepatitis C virus; RNA interference; Zika virus; biosecurity; hog cholera; industry; morbidity; mortality; swine; vaccination; viruses
Abstract:
... Classical swine fever (CSF), caused by CSF virus (CSFV), is a highly contagious swine disease with high morbidity and mortality, which has caused significant economic losses to the pig industry worldwide. Biosecurity measures and vaccination are the main methods for prevention and control of CSF since no specific drug is available for the effective treatment of CSF. Although a series of biosecurit ...
DOI:
10.3390/microorganisms9040761

16. Comparative study on combustion and emissions between methanol port-injection engine and methanol direct-injection engine with H2-enriched port-injection under lean-burn conditions

Author:
Lin Yi,   et al.   ; Changming Gong; Zhaohui Li; Fenghua Liu;     Show all 4 Authors
Source:
Energy conversion and management 2019 v.200 pp. -
ISSN:
0196-8904
Subject:
air; combustion; comparative study; emissions; hydrogen; methanol; soot
Abstract:
... Lean-burn is an effective way to improve thermal efficiency and reduce emissions of spark-ignition (SI) engines. A hydrogen (H2) enrichment combustion strategy can extend the lean-burn limits of SI engines. Two engines equipped with hydrogen and methanol dual-port injection (HPI + MPI) and hydrogen port injection plus methanol direct injection (HPI + MDI) were used to evaluate the lean-burn capabi ...
DOI:
10.1016/j.enconman.2019.112096

17. Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry

Author:
Lin Yi,   et al.   ; Lei Chen; Yilan Ouyang; Na Yan; Yan Guo; Yuanyuan Sun; Dehua Liu; Zhenqing Zhang;     Show all 8 Authors
Source:
Journal of chromatography 2021 v.1643 pp. 462049
ISSN:
0021-9673
Subject:
anion exchange; gel chromatography; heart; heparin; heparin lyase; mass spectrometry
Abstract:
... Heparin is a linear sulfated polysaccharide. It is composed of a repeating disaccharide unit with different sulfo patterns. The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure. Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stabil ...
DOI:
10.1016/j.chroma.2021.462049

18. Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish

Author:
Ti‐Lin Yi,   et al.   ; Meng‐Ting Pei; Dai‐Qin Yang;     Show all 3 Authors
Source:
FEBS Open Bio 2019 v.9 no.10 pp. 1835-1844
ISSN:
2211-5463
Subject:
Monopterus albus; brain; fish; genome; models; protogyny; sex reversal
Abstract:
... Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals, fishes often possess more than one copy of the kiss and gpr54 genes. Our objecti ...
DOI:
10.1002/2211-5463.12727

19. Advances and applications of in-tube solid-phase microextraction for analysis of proteins

Author:
Lin-Yi Dong,   et al.   ; Liang Xu; Zhan-Song Hu; Rui Duan; Xuan Wang; Yuan-Shuo Yang; Xian-Hua Wang;     Show all 7 Authors
Source:
Journal of chromatography 2021 v.1640 pp. 461962
ISSN:
0021-9673
Subject:
cell membranes; chromatography; lectins; lysine; medicine; molecular imprinting; oligonucleotides; quantitative analysis; solid phase microextraction
Abstract:
... In-tube solid-phase microextraction (IT-SPME) with capillary column as extraction device is a well-established green extraction technique with a lot of applications in the fields of biomedicine, food and environment. This article reviews the research contributions of IT-SPME for analysis of proteins. The paper first briefly describes the history of IT-SPME. Then, the development and principle of I ...
DOI:
10.1016/j.chroma.2021.461962

20. Preparation of magnetic mesoporous epoxy resin by initiator-free ring-opening polymerization for extraction of bile acids from human serum

Author:
Lin-Yi Dong,   et al.   ; Lu Sun; Rui Duan; Yu Fan; Xin-Zhu Chen; Chao Peng; Chao Zheng; Xian-Hua Wang;     Show all 8 Authors
Source:
Journal of chromatography 2020 v.1609 pp. 460448
ISSN:
0021-9673
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adsorption; bile acids; blood serum; chemical bonding; detection limit; epoxides; humans; hydrophobicity; liquid chromatography; magnetic properties; magnetism; nanoparticles; polymerization; porous media; quantitative analysis; solid phase extraction; sorbents; tandem mass spectrometry; thermal stability; thermogravimetry; transmission electron microscopes; transmission electron microscopy
Abstract:
... In this work, we reported a simple two-step method for the synthesis of magnetic mesoporous epoxy resin (MMER), including one-pot template-free hydrothermal synthesis of nanoscale amine-functionalized magnetic nanoparticles (MN-NH₂) and initiator-free ring-opening polymerization of epoxy resin. The resultant MMER was characterized in detail by transmission electron microscope (TEM), Fourier transf ...
DOI:
10.1016/j.chroma.2019.460448