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- Materne, Eva-Maria, et al. Show all 8 Authors
- Journal of biotechnology 2015 v.212 pp. 134-143
- cyclophilins; drugs; extracellular matrix; fluorescence microscopy; genes; green fluorescent protein; human cell lines; humans; liver; models; portal vein; quantitative polymerase chain reaction; rats; small interfering RNA; tissue repair; toxicity; toxicology
- ... Reconstituted three-dimensional (3D) liver models obtained by engrafting hepatic cells into an extracellular matrix (ECM) are valuable tools to study tissue regeneration, drug action and toxicology ex vivo. The aim of the present study was to establish a system for the functional investigation of a viral vector in a 3D liver model composed of human HepG2 cells on a rat ECM. An adeno-associated vir ...
- Materne, Eva-Maria, et al. Show all 10 Authors
- Journal of biotechnology 2015 v.205 pp. 36-46
- animal tests; apoptosis; clinical trials; coculture; drug toxicity; drugs; fluorescent antibody technique; glucose; humans; laboratory animals; lactate dehydrogenase; lactic acid; liver; neurons; neurotoxicity; phenotype; phylogeny; quantitative polymerase chain reaction; staining; tissues; viability
- ... Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, often do not accurately predict drug toxicity, leading to high attrition rates in clinical studies (Paul et al., 2010). The phylogenetic distance between humans and laboratory animals is enormous, this affects the transferability of animal data on the effica ...