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- McConnell, W., et al. Show all 6 Authors
- Veterinary microbiology 2010 v.144 no.1-2 pp. 226-230
- cattle; cattle diseases; botulism; Clostridium botulinum; pathotypes; bacterial toxins; neurotoxins; disease detection; screening; intestines; in vitro culture; new methods; enrichment culture; heat treatment; monoclonal antibodies; enzyme-linked immunosorbent assay; validity; disease diagnosis; optimization; mice; bioassays; animal use refinement; animal use reduction
- ... Monoclonal antibodies (MAbs) obtained from a mouse immunised with Clostridium botulinum type D toxoid were developed into a sandwich ELISA (sELISA) format that was able to detect type D toxin and types C and D toxin complexes. The sELISA was examined for its potential to replace the mouse bioassay as an alternative in vitro assay for the diagnosis of cattle botulism. Its application directly to in ...