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1. Comparing the kinetics of the hydrolysis of by-product from channel catfish (Ictalurus punctatus) fillet processing by eight proteases

Author:
Meng, Shi,   et al.   ; Tan, Yuqing; Chang, Sam K.C.;     Show all 3 Authors
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.111 pp. 809-820
ISSN:
0023-6438
Subject:
Ictalurus punctatus; bromelains; byproducts; catfish; emulsifying; engineering; enzymatic hydrolysis; enzyme activity; ficain; fillets; fish culture; foaming; hydrolysates; hydrolysis; models; papain; proteolysis; rural economics; subtilisin; temperature; thermolysin; United States
Abstract:
... Channel catfish farming is the most important warm water aquaculture in the United States and is very important to the rural economy. This study's objective was to investigate the kinetics of the enzymatic hydrolysis of catfish by-product. Channel catfish (Ictalurus punctatus) by-products (heads and frames) were collected from local catfish processing plant. Papain, ficin, bromelain, neutrase, alc ...
DOI:
10.1016/j.lwt.2019.05.053

2. A VIT‐like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean

Author:
Zhang, Meng Shi,   et al.   ; Liu, Sheng; Liao, Li Li; Nie, Miao Miao; Peng, Wen Ting; Lei, Jia Ning; Zhong, Yong Jia; Liao, Hong; Chen, Zhi Chang;     Show all 9 Authors
Source:
Thenew phytologist 2020 v.226 no.5 pp. 1413-1428
ISSN:
0028-646X
Subject:
Glycine max; cytosol; gene expression regulation; growth retardation; iron; knockout mutants; nitrogen fixation; phenotype; plant root cells; proteins; protoplasts; sap; soybeans; symbiosis; tobacco; tonoplast; vacuoles; yeasts
Abstract:
... Effective legume–rhizobia symbiosis depends on efficient nutrient exchange. Rhizobia need to synthesize iron‐containing proteins for symbiotic nitrogen fixation (SNF) in nodules, which depends on host plant‐mediated iron uptake into the symbiosome. We functionally investigated a pair of vacuolar iron transporter like (VTL) genes, GmVTL1a/b, in soybean (Glycine max) and evaluated their contribution ...
DOI:
10.1111/nph.16506

3. Forming a Large-Scale Droplet Array in a Microcage Array Chip for High-Throughput Screening

Author:
Huang, Meng-Shi,   et al.   ; Xu, Jin-Gang; Wang, Hui-Feng; Fang, Qun;     Show all 4 Authors
Source:
Analytical chemistry 2019 v.91 no.16 pp. 10757-10763
ISSN:
1520-6882
Subject:
Escherichia coli; bacteria; cell viability; chemical reactions; culture media; directed evolution; droplets; drugs; esterases; fluorescence; fluorescence microscopes; fluorescence microscopy; oils; rapid methods; screening
Abstract:
... Forming a large-scale droplet array plays an important role for microfluidic droplet-based high-throughput screening and analysis. Herein, we describe a simple and rapid method to form a large-scale two-dimension (2D) droplet array by using a microcage array chip. Differing from the previous droplet array formation methods, microcages formed by being surrounded by multiple micropillars could rapid ...
DOI:
10.1021/acs.analchem.9b02288

4. Peanut Allergen Reduction and Functional Property Improvement by Means of Enzymatic Hydrolysis and Transglutaminase Crosslinking

Author:
Meng, Shi,   et al.   ; Tan, Yuqing; Chang, Sam; Li, Jiaxu; Maleki, Soheila; Puppala, Naveen;     Show all 6 Authors
Source:
Food chemistry 2019 pp. 125186
ISSN:
0308-8146
Subject:
allergenicity; allergens; bromelains; crosslinking; enzymatic hydrolysis; ficain; functional properties; hydrolysates; hydrolysis; immunoglobulin E; kinetics; papain; peanut protein; peanuts; protein hydrolysates; protein-glutamine gamma-glutamyltransferase
Abstract:
... Enzymatic processing could reduce the allergenicity of peanut proteins while may lose the functional properties. Transglutaminase (TGase) is an enzyme for improving the functional properties of proteins/hydrolysates. No studies have been conducted on peanut hydrolysates that are crosslinked with TGase. In this study, allergenicity and functional properties of peanut protein hydrolysate cross-linke ...
DOI:
10.1016/j.foodchem.2019.125186

5. FLIM–FRET-Based Structural Characterization of a Class-A GPCR Dimer in the Cell Membrane

Author:
Yang, Meng-Shi,   et al.   ; Yang, Ju; Gong, Zhou; Lu, Yun-Bi; Xu, Chan-Juan; Wei, Tao-Feng; Zhan, Tian-Wei; Yang, Yu-Hong; Lin, Li; Liu, Jianfeng; Tang, Chun; Zhang, Wei-Ping;     Show all 12 Authors
Source:
Journal of molecular biology 2020 v.432 no.16 pp. 4596-4611
ISSN:
0022-2836
Subject:
G-protein coupled receptors; agonists; cell membranes; crosslinking; dimerization; fluorescence microscopy; mitogen-activated protein kinase; models; mutants; mutation
Abstract:
... Class-A G protein-coupled receptors (GPCRs) are known to homo-dimerize in the membrane. Yet, methods to characterize the structure of GPCR dimer in the native environment are lacking. Accordingly, the molecular basis and functional relevance of the class-A GPCR dimerization remain unclear. Here, we present the dimeric structural model of GPR17 in the cell membrane. The dimer mainly involves transm ...
DOI:
10.1016/j.jmb.2020.06.009

6. Immunomodulatory effects of Trichinella spiralis excretory-secretory antigens on macrophages

Author:
Meng, Shi,   et al.   ; Han, Caixia; Yu, Jie; Zhang, Ziqun; Zhai, Peng; Zhang, Yan; Yu, Yang; Li, Xiaoyun; Song, Mingxin;     Show all 9 Authors
Source:
Experimental parasitology 2019 v.196 pp. 68-72
ISSN:
0014-4894
Subject:
Trichinella spiralis; Western blotting; antigens; excretory-secretory products; helminths; immune response; immunomodulators; interleukin-10; interleukin-12; macrophages; tumor necrosis factor-alpha
Abstract:
... Helminths and their products can suppress the host immune response and other immunopathologies which may benefit parasite survival. Parasitic nematode Trichinella spiralis (T. spiralis) exert immunomodulatory effect on the host immune response through excretory-secretory (ES) products. However, the immunomodulatory mechanism is not yet completely understood or defined. Macrophages play a key role ...
DOI:
10.1016/j.exppara.2018.10.001

7. Protein and quality analyses of accessions from the USDA soybean germplasm collection for tofu production

Author:
Meng, Shi,   et al.   ; Chang, Sam; Gillen, Anne M.; Zhang, Yan;     Show all 4 Authors
Source:
Food chemistry 2016 v.213 pp. 31-39
ISSN:
0308-8146
Subject:
USDA; chemical composition; firmness; food industry; food quality; foods; genotype; germplasm; prediction; sensory properties; soybeans; texture; tofu; Mississippi
Abstract:
... Food-grade soybeans with large seed size, uniformity, clear hilum and a high 11S/7S ratio are favoured by the food industry for making tofu. In order to search for soybean lines with desirable characteristics for making foods, 22 soybean lines were selected from the USDA-Soybean Germplasm Collection, were grown in Stoneville, MS for biochemical analysis and tofu texture and sensory quality tests. ...
DOI:
10.1016/j.foodchem.2016.06.046
Chorus Open Access:
10.1016/j.foodchem.2016.06.046

8. Taxonomic status and phylogenetic position of Phaeostigma in the subtribe Artemisiinae (Asteraceae)

Author:
Meng, Shi‐Yong,   et al.   ; Huang, Yuan; An, Yi‐Ming; Guo, Yan‐Ping; Rao, Guang‐Yuan;     Show all 5 Authors
Source:
Journal of systematics and evolution 2017 v.55 no.5 pp. 426-436
ISSN:
1674-4918
Subject:
Asteraceae; branches; chloroplast DNA; environmental factors; flowering; genes; internal transcribed spacers; leaves; loci; monophyly; organisms; pollen; ribosomal DNA; scanning electron microscopy; transcription (genetics)
Abstract:
... Phaeostigma is a controversial taxon for its taxonomic status and phylogenetic position in the subtribe Artemisiinae, Asteraceae. To address those questions, a phylogenetic evaluation of Phaeostigma and its related genera of subtribe Artemisiinae was carried out based on molecular as well as morphological character data. DNA sequences of nuclear ribosomal DNA internal transcribed spacer, the singl ...
DOI:
10.1111/jse.12257

9. Light and Magnetic Dual-Responsive Pickering Emulsion Micro-Reactors

Author:
Meng, Shi-Xin,   et al.   ; Xie, Chun-Yan; Xue, Long-Hui; Bai, Rui-Xue; Yang, Xin; Wang, Yaolei; Qiu, Zhong-Ping; Binks, Bernard P.; Guo, Ting; Meng, Tao;     Show all 10 Authors
Source:
Langmuir 2017 v.33 no.49 pp. 14139-14148
ISSN:
1520-5827
Subject:
chemical reactions; droplets; emulsions; encapsulation; hydrophilicity; hydrophobicity; iron oxides; magnetic fields; nanoparticles; oil-water interface; titanium dioxide; ultraviolet radiation
Abstract:
... Emulsion droplets can serve as ideal compartments for reactions. In fact, in many cases, the chemical reactions are supposed to be triggered at a desired position and time without change of the system environment. Here, we present a type of light and magnetic dual-responsive Pickering emulsion microreactor by coadsorption of light-sensitive titania (TiO₂) and super paramagnetic iron oxide (Fe₃O₄) ...
DOI:
10.1021/acs.langmuir.7b03642

10. Preparation and evaluation of a sustained‐release buckwheat noodle

Author:
Chen, Meng‐Shi,   et al.   ; Wang, Shao‐Yun; Huang, Qi‐Min; Lin, Yue‐Ping; Rao, Ping‐Fan; Wu, Yan; Wu, Jin‐Hong;     Show all 7 Authors
Source:
Journal of the science of food and agriculture 2016 v.96 no.8 pp. 2660-2667
ISSN:
0022-5142
Subject:
Amorphophallus; buckwheat; buckwheat flour; colloids; corn starch; digestibility; digestion; equations; glucose; gluten; hydrophilicity; manufacturing; models; noodles; starch granules; xanthan gum
Abstract:
... BACKGROUND: Different carbohydrates elicit various effects on the digestibility and the glucose release rate, so it is of interest to develop a sustained‐release noodle based on the combination of different carbohydrates and reveal the sustained‐release mechanism. RESULTS: The data obtained suggest that xanthan and konjac gum exhibited excellent and synergistic sustained‐release properties, wherea ...
DOI:
10.1002/jsfa.7383

11. A novel domino approach for synthesis of indolyl tetrahydropyrano[4,3-c]pyrazole derivatives as anticancer agents

Author:
Meng, Shi-Yao,   et al.   ; Wang, Fu-Qiang; Yang, Hui; He, Bin; Jia, Yong-Kang; Zhang, Chao; Liu, Hong-Min; Liu, Feng-Wu;     Show all 8 Authors
Source:
Tetrahedron 2016 v.72 pp. 5769-5775
ISSN:
0040-4020
Subject:
antineoplastic activity; chemical reactions; chemical structure; doxorubicin; fluorouracil; humans; neoplasms
Abstract:
... A series of indolyl substituted 1,4,6,7-tetrahydropyrano[4,3-c]pyrazole derivatives were synthesized via a domino method based on the 1,4:3,6-Dianhydrofructose. The mechanism was proposed and proved by calculation with a density functional theory (DFT), which includes formation of phenylhydrazone, furanone ring opening, Fischer indole synthesis, pyrazole ring closing and a furan ring expansion. In ...
DOI:
10.1016/j.tet.2016.07.078

12. Light-Triggered Release from Pickering Emulsions Stabilized by TiO2 Nanoparticles with Tailored Wettability

Author:
Meng, Shi-Xin,   et al.   ; Bai, Rui-Xue; Xue, Long-Hui; Dou, Rong-Kun; Xie, Chun-Yan; Zhang, Qing; Guo, Ting; Meng, Tao;     Show all 8 Authors
Source:
Langmuir 2016 v.32 no.36 pp. 9254-9264
ISSN:
1520-5827
Subject:
droplets; emulsifiers; emulsions; hydrophilicity; irradiation; light intensity; microencapsulation; nanoparticles; nitrogen; oils; titanium dioxide; wavelengths; wettability
Abstract:
... In this work, a new strategy for developing light-triggered Pickering emulsions as smart soft vehicles for on-demand release is proposed. Initially, UV-induced tailored wettability allows anchoring of TiO₂ nanoparticles at the interface to prepare stable water in oil emulsions. Such emulsions show the efficacy of microencapsulation and controlled release by demulsification due to the hydrophilic c ...
DOI:
10.1021/acs.langmuir.6b02329

13. Rapid radiation and dispersal out of the Qinghai-Tibetan Plateau of an alpine plant lineage Rhodiola (Crassulaceae)

Author:
Meng, Shi-Yong,   et al.   ; Zhang, Jian-Qiang; Allen, Geraldine A.; Wen, Jun; Rao, Guang-Yuan;     Show all 5 Authors
Source:
Molecular phylogenetics and evolution 2014 v.77 pp. 147-158
ISSN:
1055-7903
Subject:
Bayesian theory; DNA fragmentation; Phedimus; Rhodiola; alpine plants; biogeography; data collection; internal transcribed spacers; phylogeny; plastid DNA; China; Europe; North America
Abstract:
... Rhodiola L. (Crassulaceae) is a mid-sized plant genus consisting of about 70 species, with most species distributed on the Qinghai-Tibetan Plateau (QTP) and the adjacent areas, and several species in north-east Asia, Europe, and North America. This study explored the origin and diversification history of Rhodiola and tested the biogeographic relationships between the QTP and other regions of the N ...
DOI:
10.1016/j.ympev.2014.04.013

14. Phylogenetic Relationships and Character Evolution of Rhodiola (Crassulaceae) based on Nuclear Ribosomal ITS and Plastid trnL-F and psbA-trnH Sequences

Author:
Meng, Shi-Yong,   et al.   ; Zhang, Jian-Qiang; Wen, Jun; Rao, Guang-Yuan;     Show all 4 Authors
Source:
Systematic botany 2014 v.39 no.2 pp. 441-451
ISSN:
0363-6445
Subject:
Rhodiola rosea; center of diversity; cold zones; dioecy; flowering; internal transcribed spacers; medicinal plants; monophyly; plant taxonomy; sampling; stems; varieties; China; Europe; North America
Abstract:
... Rhodiola (Crassulaceae) comprises about 70 species and shows a high level of morphological diversity. The genus is mainly distributed in alpine areas and cold regions of the Northern Hemisphere with the Qinghai-Tibetan Plateau as its center of diversity. The phylogenetic relationships within Rhodiola remain poorly understood largely because of difficulties in collecting samples and specimens. In t ...
DOI:
10.1600/036364414X680753

15. Effects of chitosan and calcium chloride treatments on malic acid-metabolizing enzymes and the related gene expression in post-harvest pear cv. ‘Huang guan’

Author:
Wu, Meng-shi,   et al.   ; Kou, Xiao-hong; Wang, Shuang; Zhang, Ying; Guo, Run-zi; Chen, Qiong; Xue, Zhao-hui;     Show all 7 Authors
Source:
Scientia horticulturae 2014 v.165 pp. 252-259
ISSN:
0304-4238
Subject:
Pyrus pyrifolia; adverse effects; calcium chloride; chitosan; cold storage; enzyme activity; flavor; gene expression; genes; juice quality; malate dehydrogenase; malic acid; malic enzyme; pears; proton pump; pulp; storage time; taste
Abstract:
... Malic acid in the ‘Huang guan’ pear (Pyrus pyrifolia Nakai) is the predominant organic acid associated with taste, flavor and juice quality. The effects of different treatments on malic acid-metabolizing enzymes and the related genes expression during storage have been studied. Malic acid content was higher in the peel than in the pulp. NAD-dependent malate dehydrogenase (NAD-MDH) and NADP-depende ...
DOI:
10.1016/j.scienta.2013.10.034

16. Carbohydrate-based furopyran-3-ones: synthesis via a rare carbonyl nucleophilic 1,2-migration induced by TMSOTf and antifungal activity

Author:
Meng, Shi-Yao,   et al.   ; Jia, Yong-Kang; Li, Ming; Han, Wei-Wei; Wang, Fu-Qiang; Zhu, Shi-Qing; Qin, Shang-Shang; Song, Hui-Ying; Hou, Yu; Shi, Xiu-Fang; Liu, Feng-Wu; Liu, Hong-Min;     Show all 12 Authors
Source:
Tetrahedron 2015 v.71 pp. 9420-9428
ISSN:
0040-4020
Subject:
Candida albicans; Candida parapsilosis; Epidermophyton floccosum; Microsporum canis; Trichophyton rubrum; animal pathogenic fungi; antifungal properties; chemical reactions; chemical structure; keratinophilic fungi; organic compounds
Abstract:
... A versatile method for synthesis of chiral furopyran-β-one analogues starting from 1,4:3,6-dianhydrofructose has been developed. The target compounds were acquired by treatment of 1,4:3,6-dianhydrofructose with benzaldehydes in the presence of KF/Al2O3 and subsequent ring expansion of furanones induced by TMSOTf (trimethylsilyl triflate). The ring expansion involves an unprecedented nucleophilic 1 ...
DOI:
10.1016/j.tet.2015.10.054

17. Cr(VI) reduction by Pseudomonas aeruginosa immobilized in a polyvinyl alcohol/sodium alginate matrix containing multi-walled carbon nanotubes

Author:
Wu, Meng-Shi,   et al.   ; Pang, Ya; Zeng, Guang-Ming; Tang, Lin; Zhang, Yi; Liu, Yuan-Yuan; Lei, Xiao-Xia; Li, Zhen; Liu, Can;     Show all 9 Authors
Source:
Bioresource technology 2011 v.102 no.22 pp. 10733-10736
ISSN:
0960-8524
Subject:
Pseudomonas aeruginosa; alcohols; carbon nanotubes; sodium alginate
Abstract:
... Pseudomonas aeruginosa (P. aeruginosa) was immobilized with polyvinyl alcohol (PVA), sodium alginate and multiwalled carbon nanotubes (MCNTs). After immobilization, the beads were subjected to freeze-thawing to enhance mechanical strength. When exposed to 80mg/L Cr(VI), the immobilized bacteria were able to reduce 50% of them in 84h, however the free cells were deactivated at this concentration. T ...
DOI:
10.1016/j.biortech.2011.08.078
PubMed:
21937224

18. Electrochemical detection of Pseudomonas aeruginosa 16S rRNA using a biosensor based on immobilized stem–loop structured probe

Author:
Wu, Meng-Shi,   et al.   ; Liu, Can; Zeng, Guang-Ming; Tang, Lin; Zhang, Yi; Li, Yuan-Ping; Liu, Yuan-Yuan; Li, Zhen; Luo, Jie;     Show all 9 Authors
Source:
Enzyme and microbial technology 2011 v.49 no.3 pp. 266-271
ISSN:
0141-0229
Subject:
DNA; Pseudomonas aeruginosa; biosensors; biotin; composting; correlation; detection limit; electrochemistry; electrodes; gold; hybridization; normal values; peroxidase; ribosomal RNA; thiols
Abstract:
... We have designed an electrochemical DNA biosensor based on stem–loop structured probes for enzymatic detection of Pseudomonas aeruginosa 16S ribosomal RNA (rRNA) in composting degradation. The probe modified with a thiol at its 5′ end and a biotin at its 3′ end was immobilized on a gold electrode through self-assembly. The stem–loop structured probes were “closed” when target was absent, then the ...
DOI:
10.1016/j.enzmictec.2011.06.011