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- Menke, Michael A., et al. Show all 9 Authors
- European journal of biochemistry 2000 v.267 no.17 pp. 5493-5501
- Escherichia coli; NADP (coenzyme); Pichia pastoris; Zygosaccharomyces rouxii; acetone; cDNA libraries; catalytic activity; complementary DNA; genes; histidine; sequence analysis; substrate specificity
- ... A novel ketoreductase isolated from Zygosaccharomycesârouxii catalyzes the asymmetric reduction of selected ketone substrates of commercial importance. The 37.8âkDa ketoreductase was purified more than 300âfold to >â95% homogeneity from whole cells with a 30% activity yield. The ketoreductase functions as a monomer with an apparent Km for 3,4âmethylenedioxyphenyl acetone of 2.9âmm and ...