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1. Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR

Author:
Miletti, Luiz Claudio,   et al.   ; Vieira, Luisa Lemos; Canever, Mariana Feltrin; Cardozo, Leonardo Leite; Cardoso, Cristina Perito; Herkenhoff, Marcos Edgar; Neto, André Thaler; Vogel, Carla Ivane Ganz;     Show all 8 Authors
Source:
Parasite epidemiology and control 2019 pp. e00114
ISSN:
2405-6731
Subject:
Anaplasma marginale; Babesia bigemina; Babesia bovis; DNA; blood; blood sampling; cattle; chloroform; disease occurrence; enzootic diseases; farms; hemoparasites; mixed infection; pathogens; phenol; polymerase chain reaction; screening; signs and symptoms (animals and humans); Brazil
Abstract:
... This study aimed to determine the prevalence of three common hemoparasites (Anaplasma marginale, Babesia bovis and Babesia bigemina) in cattle from 16 counties in the Campos de Lages region, Santa Catarina state, Brazil, and the factors affecting disease occurrence. The study population consisted of 257 clinically healthy animals from 21 rural farms. Bovine blood samples were collected by jugular ...
DOI:
10.1016/j.parepi.2019.e00114

2. Effects of resveratrol on the differentiation fate of neural progenitor cells of mouse embryos infected with Trypanosoma cruzi

Author:
Miletti, Luiz Claudio,   et al.   ; Fracasso, Mateus; Bottari, Nathieli B.; da Silva, Aniélen D.; Grando, Thirssa H.; Pillat, Micheli M.; Ulrich, Henning; Vidal, Tais; de Andrade, Cinthia M.; Monteiro, Silvia G.; Nascimento, Luiz Flavio N.; Schafer da Silva, Aleksandro;     Show all 12 Authors
Source:
Microbial pathogenesis 2019 v.132 pp. 156-161
ISSN:
0882-4010
Subject:
Chagas disease; Trypanosoma cruzi; antioxidant activity; blood-brain barrier; brain; cell cycle; cognition; embryo (animal); females; grapes; medicinal properties; mice; neural stem cells; neurogenesis; neurons; parasites; polyphenols; resveratrol; therapeutics
Abstract:
... Chagas disease (CD) affecting about 7 million people is caused by the flagellate protozoan Trypanosoma cruzi. The central nervous system (CNS) is an important site for T. cruzi persistence in the host during the chronic phase of infection, because the protozoan may pass the blood-brain barrier and may cause motor and cognitive neuronal damage. Thinking about avoiding or minimizing these negative e ...
DOI:
10.1016/j.micpath.2019.04.040

3. Growth of juvenile common snook (Centropomus undecimalis Bloch, 1792) reared at different temperatures and salinities: Morphometric parameters, RNA/DNA, and protein/DNA ratios

Author:
Miletti, Luiz Cláudio,   et al.   ; Costa Filho, João; Tsuzuki, Mônica Yumi; Mello, Giovanni Lemos de; Lüchmann, Karim Hahn; Lima-Rosa, Carlos André da Veiga;     Show all 6 Authors
Source:
Journal of applied aquaculture 2017 v.29 no.3-4 pp. 199-206
ISSN:
1545-0805
Subject:
Centropomus; DNA; RNA; juveniles; morphometry; rearing; salinity; temperature
Abstract:
... We investigated the growth of juvenile common snook (Centropomus undecimalis) reared at 25°C and 28°C and salinities of 0.3, 15, and 32 g L ⁻¹. Total length, weight, RNA/DNA, and protein/DNA ratios were determined after 90 days of experiment. Higher growth was observed at 28ºC compared with 25°C, at the same salinity. At 28°C and 15 g L ⁻¹ salinity, the weight (25.14 g) of juveniles was twice that ...
DOI:
10.1080/10454438.2017.1334615

4. Vertical transmission of Trypanosoma evansi in experimentally infected rats

Author:
Miletti, Luiz Claudio,   et al.   ; Campigotto, Gabriela; Volpato, Andréia; Galli, Gabriela M.; Glombowsky, Patrícia; Baldissera, Matheus D.; Jaguezeski, Antonise M.; Stefani, Lenita M.; Da Silva, Aleksandro S.;     Show all 9 Authors
Source:
Experimental parasitology 2017 v.174 pp. 42-44
ISSN:
0014-4894
Subject:
Trypanosoma evansi; adults; blood; blood sampling; death; females; fetal death; males; parasitemia; parasites; polymerase chain reaction; pregnancy; progeny; puppies; pups; rats; transplacental transmission
Abstract:
... Many reproductive problems has been described in male and female animals infected by Trypanosoma evansi. Thus, the aim of this study was to evaluate the occurrence of vertical (Experiment I) and venereal (Experiment II) transmission of T. evansi in rats experimentally infected. In the experiment I, eight female Wistar rats were used: three animals as negative controls, and five rats were infected ...
DOI:
10.1016/j.exppara.2017.01.012

5. Kinetic and biochemical characterization of Trypanosoma evansi nucleoside triphosphate diphosphohydrolase

Author:
Miletti, Luiz Claudio,   et al.   ; Weiss, Paulo Henrique Exterchoter; Batista, Franciane; Wagner, Glauber; Magalhães, Maria de Lourdes Borba;     Show all 5 Authors
Source:
Experimental parasitology 2015 v.153 pp. 98-104
ISSN:
0014-4894
Subject:
Trypanosoma brucei; Trypanosoma evansi; bioinformatics; carbohydrates; cations; enzyme activity; enzymes; eukaryotic cells; genes; hemostasis; host-pathogen relationships; hydrolysis; inflammation; nucleosides; nucleotide sequences; parasites; sodium; substrate specificity; virulence
Abstract:
... Nucleoside triphosphate diphospho-hydrolases (NTPDases) catalyze the hydrolysis of several nucleosides tri and diphosphate playing major roles in eukaryotes including purinergic signaling, inflammation, hemostasis, purine salvage and host–pathogen interactions. These enzymes have been recently described in parasites where several evidences indicated their involvement in virulence and infection. He ...
DOI:
10.1016/j.exppara.2015.03.009

6. Rapid Detection of Mycoplasma synoviae and Avian Reovirus in Clinical Samples of Poultry Using Multiplex PCR

Author:
Miletti, Luiz Claudio,   et al.   ; Reck, Carolina; Menin, Álvaro; Canever, Marina Feltrin;     Show all 4 Authors
Source:
Avian diseases 2013 v.57 no.2 pp. 220-224
ISSN:
1938-4351
Subject:
Mycoplasma synoviae; Reoviridae; birds; detection limit; diagnostic techniques; disease control; disease course; flocks; fluorescent antibody technique; monitoring; pathogens; polymerase chain reaction; poultry; poultry diseases; rapid methods; screening
Abstract:
... Mycoplasma synoviae and avian reovirus (ARV) are associated with several disease syndromes in poultry and cause notable global economic losses in the poultry industry. Rapid and efficient diagnostics for these avian pathogens are important not only for disease control but also for prevention of clinical disease progression. However, current diagnostic methods used for surveillance of these disease ...
DOI:
10.1637/10425-101712-Reg.1

7. Natural Infection of the Wild Canid Lycalopex gymnocercus by the Protozoan Rangelia vitalii, the Agent of Canine Rangeliosis

Author:
Miletti, Luiz Claudio,   et al.   ; de Quadros, Rosiléia Marinho; Soares, João Fábio; Xavier, Juliane Scharlau; Pilati, Celso; da Costa, Jocelim Lotário; Miotto, Bruno Alonso; Labruna, Marcelo B.;     Show all 8 Authors
Source:
Journal of wildlife diseases 2015 v.51 no.3 pp. 787-789
ISSN:
0090-3558
Subject:
Capillaria hepatica; Hepatozoon canis; Lycalopex gymnocercus; Protozoa; dogs; foxes; South America
Abstract:
... Rangelia vitalii is a piroplasmid that causes canine rangeliosis, a severe hemorrhagic disease of domestic dogs in South America. We report about R. vitalii infecting a pampas fox (Lycalopex gymnocercus). The fox, which developed a fatal illness, was also infected by Hepatozoon canis and Capillaria hepatica. ...
DOI:
10.7589/2014-08-194

8. Prevalence of Eimeria spp. in Broilers by Multiplex PCR in the Southern Region of Brazil on Two Hundred and Fifty Farms

Author:
Miletti, Luiz Claudio,   et al.   ; Moraes, Julio Cesar; França, Marciél; Sartor, Amélia Aparecida; Bellato, Valdomiro; de Moura, Anderson Barbosa; Magalhães, Maria de Lourdes Borba; de Souza, Antonio Pereira;     Show all 8 Authors
Source:
Avian diseases 2015 v.59 no.2 pp. 277-281
ISSN:
1938-4351
Subject:
Eimeria acervulina; Eimeria brunetti; Eimeria maxima; Eimeria mitis; Eimeria necatrix; Eimeria praecox; Eimeria tenella; bird diseases; broiler chickens; clones; coccidiosis; disease control; disease prevalence; farms; feces; flocks; microscopy; oocysts; polymerase chain reaction; poultry industry; poultry production; Brazil
Abstract:
... Parasitic infections caused by Eimeria species are responsible for most economic losses in poultry production. Prevalence studies can adequately assist the design of prophylaxis strategies for disease control. Therefore, stool samples from 251 flocks of broilers from 28 to 48 days old were collected in 21 municipalities in the state of Santa Catarina, Brazil, to detect and examine the prevalence o ...
DOI:
10.1637/10989-112014-Reg

9. Glutathione and iron at the crossroad of redox metabolism in rats infected by Trypanosoma evansi

Author:
Miletti, Luiz Claudio,   et al.   ; Anschau, Valesca; Dafré, Alcir Luiz; Perin, Ana Paula; Iagher, Fabíola; Tizatto, Mayara Vieira;     Show all 6 Authors
Source:
Parasitology research 2013 v.112 no.6 pp. 2361-2366
ISSN:
0932-0113
Subject:
Trypanosoma evansi; blood composition; correlation; erythrocytes; glutathione; hematocrit; iron; lipid peroxidation; metabolism; oxidative stress; parasitemia; rats
Abstract:
... The aim of this study was to evaluate the changes in hematological and biochemical parameters of blood during acute Trypanosoma evansi infection in Wistar rats. The end points studied were hematologic parameters, red blood cell fragility, iron content, and glutathione and lipid peroxidation levels. Forty-eight animals were infected with trypomastigotes and distributed into five groups according to ...
DOI:
10.1007/s00436-013-3400-9
PubMed:
23529337

10. Cryopreservation of Trypanosoma evansi after DEAE-cellulose purification: Evaluation of infective parameters

Author:
Miletti, Luiz Claudio,   et al.   ; Tavares, Kaio César Simano; Da Silva, Aleksandro Schafer; Wolkmer, Patricia; Monteiro, Silvia Gonzalez;     Show all 5 Authors
Source:
Research in veterinary science 2011 v.90 no.2 pp. 257-259
ISSN:
0034-5288
Subject:
Trypanosoma evansi; chromatography; cryopreservation; in vitro culture; longevity; molecular biology; parasitemia; parasites; prepatent period; storage
Abstract:
... Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4months has been described. In this study, we examined the effect of purifying trypomastigote throu ...
DOI:
10.1016/j.rvsc.2010.05.021
PubMed:
20542526

11. Susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium: Test in experimentally infected mice

Author:
Miletti, Luiz Claudio,   et al.   ; Faccio, Luciana; Da Silva, Aleksandro S.; Gressler, Lucas T.; Tonin, Alexandre A.; Lazzarotto, Cícera R.; Monteiro, Silvia G.;     Show all 7 Authors
Source:
Experimental parasitology 2013 v.134 no.3 pp. 309-312
ISSN:
0014-4894
Subject:
Trypanosoma evansi; blood; drug resistance; mice; parasitemia; parasitology; polymerase chain reaction; sodium; suramin
Abstract:
... This study aimed to evaluate the susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium. For this purpose, three isolates of T. evansi (LPV-2005, LPV-2009 and LPV-2010) and seventy mice were used, with the animals divided in 10 groups (A, B, C, D, E, F, G, H, I and J) with seven animals each group. Mice of groups A, B, and C were infected with LPV-2005; Groups D, E and F with ...
DOI:
10.1016/j.exppara.2013.03.027

12. Occurrence of Trypanosoma evansi in Horses in the State of Minas Gerais, Brazil

Author:
Miletti, Luiz Claudio,   et al.   ; Nunes, João Tomaz Schmitt; da Silva, Aleksandro Schafer; de Souza Dorneles, Fellipe; Tonin, Alexandre Alberto; Lazzarotto, Cícera; Monteiro, Silvia Gonzalez;     Show all 7 Authors
Source:
Journal of equine veterinary science 2012 v.32 no.4 pp. 205-207
ISSN:
0737-0806
Subject:
Trypanosoma evansi; anemia; ataxia (disorder); blood; diminazene; edema; farmers; farms; lameness; muscular atrophy; parasites; polymerase chain reaction; stallions; trypanosomiasis; weight loss; Brazil
Abstract:
... Ttrypanosomosis caused by Trypanosoma evansi is a condition that causes significant losses to farmers in endemic areas. This study aimed to report one case of trypanosomiasis in the municipality of Itabira, state of Minas Gerais, Brazil. In November 2010, on a farm with 16 horses, three horses had clinical signs of anemia, limb edema, weight loss, lameness, muscle atrophy, and incoordination of th ...
DOI:
10.1016/j.jevs.2011.08.023

13. Trypanosoma rangeli expresses a β-galactofuranosyl transferase

Author:
Miletti, Luiz Claudio,   et al.   ; Stoco, Patrícia Hermes; Aresi, Cassandra; Lückemeyer, Débora Denardin; Sperandio, Maísa Michels; Sincero, Thaís Cristine Marques; Steindel, Mário; Grisard, Edmundo Carlos;     Show all 8 Authors
Source:
Experimental parasitology 2012 v.130 no.3 pp. 246-252
ISSN:
0014-4894
Subject:
Golgi apparatus; Leishmania; Trypanosoma cruzi; Trypanosoma rangeli; antibodies; biochemical pathways; epimastigotes; genes; glycoconjugates; glycosyltransferases; hosts; insect vectors; mammals; open reading frames; parasites; parasitology; pathogenicity; sequence homology; trypomastigotes
Abstract:
... Glycoconjugates play essential roles in cell recognition, infectivity and survival of protozoan parasites within their insect vectors and mammalian hosts. β-galactofuranose is a component of several glycoconjugates in many organisms, including a variety of trypanosomatids, but is absent in mammalian and African trypanosomes. Herein, we describe the presence of a β(1–3) galactofuranosyl transferase ...
DOI:
10.1016/j.exppara.2011.12.005

14. Trypanosoma evansi: Effects of zinc and copper in experimentally infected rats

Author:
Miletti, Luiz Claudio,   et al.   ; Dalla Rosa, Luciana; Da Silva, Aleksandro S.; Oliveira, Camila B.; Brum, Isabela; Benevenutti, Érika; Dorneles, Fellipe; Jaques, Jeandre A.; Tavares, Kaio C.S.; Leal, Marta R.; Monteiro, Silvia G.;     Show all 11 Authors
Source:
Experimental parasitology 2012 v.131 no.3 pp. 358-362
ISSN:
0014-4894
Subject:
Trypanosoma evansi; blood; copper; parasitemia; parasitology; polymerase chain reaction; prepatent period; rats; zinc
Abstract:
... The aim of this study was to evaluate the effects of a treatment using injectable zinc and copper in rats infected with Trypanosoma evansi. 48 rats were divided into eight groups of six animals each. Group A was composed of uninfected animals. Animals from groups B–H were inoculated at the 5th day of experiment with 1.2×10⁶ trypanosomes. Group B was used as a positive control. The infected groups ...
DOI:
10.1016/j.exppara.2012.05.004

15. Susceptibility of Trypanosoma evansi to human blood and plasma in infected mice

Author:
Miletti, Luiz Claudio,   et al.   ; Otto, Mateus Anderson; Silva, Aleksandro Schafer da; Gressler, Lucas Trevisan; Farret, Matheus Hilliard; Tavares, Kaio César Simiano; Zanette, Régis Adriel; Monteiro, Silvia Gonzalez;     Show all 8 Authors
Source:
Veterinary parasitology 2010 v.168 no.1-2 pp. 1-4
ISSN:
0304-4017
Subject:
mice; Trypanosoma evansi; therapeutics; animal models; disease resistance; blood plasma; zoonoses; disease control
Abstract:
... Around 1900 Laveran and Mesnil discovered that African trypanosomes do not survive in the blood of some primates and humans. The nature of the trypanolytic factor present in these sera has been the focus of a long-standing debate between different groups. The aim of this study was to investigate the susceptibility of T. evansi isolates to therapy using human blood and plasma in experimentally infe ...
DOI:
10.1016/j.vetpar.2009.10.020
PubMed:
19939570