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- Miller, A. Dusty, et al. Show all 4 Authors
- Nucleic acids research 2011 v.39 no.3 pp. 926-935
- Dependoparvovirus; mutation; mutagens; plasmids; homologous recombination; gene targeting; transfection; genes; toxicity
- ... Gene targeting by homologous recombination (HR) can be induced by double-strand breaks (DSBs), however these breaks can be toxic and potentially mutagenic. We investigated the I-AniI homing endonuclease engineered to produce only nicks, and found that nicks induce HR with both plasmid and adeno-associated virus (AAV) vector templates. The rates of nick-induced HR were lower than with DSBs (24-fold ...
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