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- Mita, Marie, et al. Show all 7 Authors
- Analytical chemistry 2019 v.91 no.7 pp. 4821-4830
- blood glucose; calcium; energy; energy metabolism; fluorescence; fluorescent proteins; glucose; human cell lines; image analysis; median effective concentration; mice; mitochondria; muscles; pharynx; plasma membrane
- ... Glucose is the most important energy source for living animals. Here, we developed a series of single fluorescent protein (FP)-based glucose indicators, named as “Green Glifons”, to understand the hierarchal and mutual relationships between molecules involved in energy metabolism. Three indicators showed a different EC₅₀ for glucose (50, 600, and 4000 μM), producing a ∼7-fold change in fluorescenc ...