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- Moskalev, Evgeny A., et al. Show all 7 Authors
- Nucleic acids research 2011 v.39 no.11 pp. e77
- DNA; DNA methylation; alleles; equations; loci; polymerase chain reaction; regression analysis
- ... DNA methylation profiling has become an important aspect of biomedical molecular analysis. Polymerase chain reaction (PCR) amplification of bisulphite-treated DNA is a processing step that is common to many currently used methods of quantitative methylation analysis. Preferential amplification of unmethylated alleles--known as PCR-bias--may significantly affect the accuracy of quantification. To d ...
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