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1. Evaluating the Conformation and Binding Interface of Cap-Binding Proteins and Complexes via Ultraviolet Photodissociation Mass Spectrometry

Author:
Murphy, Patricia A.,   et al.   ; O’Brien, John P.; Mayberry, Laura K.; Browning, Karen S.; Brodbelt, Jennifer S.;     Show all 5 Authors
Source:
Journal of Proteome Research 2013 v.12 no.12 pp. 5867-5877
ISSN:
1535-3907
Subject:
Triticum aestivum; binding sites; crystal structure; dithiothreitol; lysine; mass spectrometry; models; photolysis; proteins; solvents; wheat
Abstract:
... We report the structural analysis of cap-binding proteins using a chemical probe/ultraviolet photodissociation (UVPD) mass spectrometry strategy for evaluating solvent accessibility of proteins. Our methodology utilized a chromogenic probe (NN) to probe the exposed amine residues of wheat eukaryotic translation initiation factor 4E (eIF4E), eIF4E in complex with a fragment of eIF4G (“mini-eIF4F”), ...
DOI:
10.1021/pr400869u

2. Oxidative stability of soybean oil in oleosomes as affected by pH and iron

Author:
Murphy, Patricia A.,   et al.   ; Kapchie, Virginie N.; Yao, Linxing; Hauck, Catherine C.; Wang, Tong;     Show all 5 Authors
Source:
Food chemistry 2013 v.141 no.3 pp. 2286-2293
ISSN:
0308-8146
Subject:
chlorides; creaming; droplets; foods; freeze drying; freezing; ingredients; iron; lipid bodies; lipid peroxidation; metal ions; oxidation; oxidative stability; pH; peroxide value; soybean oil; thiobarbituric acid-reactive substances
Abstract:
... The oxidative stability of oil in soybean oleosomes, isolated using the Enzyme-Assisted Aqueous Extraction Process (EAEP), was evaluated. The effects of ferric chloride, at two concentration levels (100 and 500μM), on lipid oxidation, was examined under pH 2 and 7. The peroxide value (PV) and thiobarbituric acid-reactive substance (TBARS) value of oil, in oleosome suspensions stored at 60°C, were ...
DOI:
10.1016/j.foodchem.2013.05.018

3. The Use of Epidemiology in Risk Assessment: Challenges and Opportunities

Author:
Murphy, Patricia A.,   et al.   ; Christensen, Krista; Christensen, Carol H.; Wright, J. Michael; Galizia, Audrey; Glenn, Barbara S.; Scott, Cheryl Siegel; Mall, Jennifer K.; Bateson, Thomas F.; Cooper, Glinda S.;     Show all 10 Authors
Source:
Human and ecological risk assessment 2015 v.21 no.6 pp. 1644-1663
ISSN:
1549-7860
Subject:
dose response; epidemiology; health hazards; human development; human health; humans; risk; risk assessment; risk assessors; toxicology
Abstract:
... The assessment of risk from environmental and occupational exposures incorporates and synthesizes data from a variety of scientific disciplines including toxicology and epidemiology. Epidemiological data have offered valuable contributions to the identification of human health hazards, estimation of human exposures, quantification of the exposure–response relation, and characterization of risks to ...
DOI:
10.1080/10807039.2014.967039

4. Isoflavone profiles of soymilk as affected by high-pressure treatments of soymilk and soybeans

Author:
Murphy, Patricia A.,   et al.   ; Jung, Stephanie; Sala, Ileana;     Show all 3 Authors
Source:
Food chemistry 2008 v.111 no.3 pp. 592-598
ISSN:
0308-8146
Subject:
soymilk; soybeans; food processing; high pressure treatment; food composition; isoflavones; extraction; heat treatment; glucosides; protein content; lipid content
Abstract:
... High hydrostatic pressure was applied to hydrated soybeans (100-700MPa, 25°C) and soymilk (400-750MPa; 25 and 75°C) to assess its effect on isoflavone content, profile and water-extractability. Neither pressure level nor initial treatment temperature affected soymilk isoflavone content. However, combined pressure and mild thermal treatment modified the isoflavone distribution. At 75°C, the isoflav ...
DOI:
10.1016/j.foodchem.2008.04.025

5. Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Author:
Murphy, Patricia A.,   et al.   ; Simons, Andrean L.; Renouf, Mathieu; Hendrich, Suzanne;     Show all 4 Authors
Source:
Journal of agricultural and food chemistry 2010 v.58 no.1 pp. 141–147
ISSN:
0021-8561
Subject:
intestinal absorption; flavonoids; feces; humans; bioavailability; men; women; in vitro studies; test meals; plant-based foods; nutrition physiology; functional foods
Abstract:
... It was hypothesized that 5,7,4′-OH-flavonoids disappeared more rapidly from human fecal incubations and were less absorbable by humans than flavonoids without 5-OH moieties. Anaerobic fecal disappearance rates over 24 h were determined for 15 flavonoids in samples from 20 men and 13 women. In these anaerobic fecal mixtures, flavonoids with 5,7,4′-OH groups, genistein, apigenin, naringenin, luteoli ...
DOI:
10.1021/jf902284u
PubMed:
19921837

6. Recovery and Functional Properties of Soy Storage Proteins from Lab- and Pilot-Plant Scale Oleosome Production

Author:
Murphy, Patricia A.,   et al.   ; Kapchie, Virginie N.; Towa, Lili T.; Hauck, Catherine C.;     Show all 4 Authors
Source:
journal of the American Oil Chemists' Society 2012 v.89 no.5 pp. 947-956
ISSN:
0003-021X
Subject:
chemical precipitation; coproducts; emulsifying; ethanol; foods; fractionation; functional properties; industrial applications; ingredients; lipid content; pH; polyacrylamide gel electrophoresis; protein content; protein solubility; soy protein isolate; storage proteins; ultrafiltration
Abstract:
... The aim of this study was to investigate soy protein recovery feasibility after lab- and pilot-plant scale oleosome isolation. The proteins were isolated by isoelectric precipitation and by ultrafiltration. The functional properties of the recovered proteins were compared to soy protein isolate produced in our laboratory. The residual lipid content in the aqueous supernatant affected the protein r ...
DOI:
10.1007/s11746-011-1976-8

7. The inhibition of lipopolysaccharide-induced macrophage inflammation by 4 compounds in Hypericum perforatum extract is partially dependent on the activation of SOCS3

Author:
Murphy, Patricia A.,   et al.   ; Huang, Nan; Rizshsky, Ludmila; Hauck, Catherine C.; Nikolau, Basil J.; Birt, Diane F.;     Show all 6 Authors
Source:
Phytochemistry 2012 v.76 pp. 106-116
ISSN:
0031-9422
Subject:
Hypericum perforatum; chlorogenic acid; cytokines; enzyme activity; ethanol; inflammation; interleukin-6; lipopolysaccharides; macrophages; microarray technology; necrosis; neoplasms; nitric oxide; prostaglandins; protein synthesis; quercetin; small interfering RNA
Abstract:
... Our previous studies found that 4 compounds, namely pseudohypericin, amentoflavone, quercetin, and chlorogenic acid, in Hypericum perforatum ethanol extract synergistically inhibited lipopolysaccharide (LPS)-induced macrophage production of prostaglandin E2 (PGE2). Microarray studies led us to hypothesize that these compounds inhibited PGE2 production by activating suppressor of cytokine signaling ...
DOI:
10.1016/j.phytochem.2011.12.001
PubMed:
22245632
PubMed Central:
PMC3294117

8. Identification of anti-inflammatory constituents in Hypericum perforatum and Hypericum gentianoides extracts using RAW 264.7 mouse macrophages

Author:
Murphy, Patricia A.,   et al.   ; Huang, Nan; Rizshsky, Ludmila; Hauck, Cathy; Nikolau, Basil J.; Birt, Diane F.;     Show all 6 Authors
Source:
Phytochemistry 2011 v.72 no.16 pp. 2015-2023
ISSN:
0031-9422
Subject:
Hypericum perforatum; anti-inflammatory activity; ethanol; herbs; macrophages; mice; necrosis; neoplasms; nitric oxide; phytochemicals; prostaglandins
Abstract:
... Hypericum perforatum (St. John’s wort) is an herb widely used as supplement for mild to moderate depression. Our prior studies established synergistic anti-inflammatory activity associated with 4 bioactive compounds in a fraction of a H. perforatum ethanol extract. Whether these 4 compounds also contributed to the ethanol extract activity was addressed in the research reported here. Despite the po ...
DOI:
10.1016/j.phytochem.2011.07.016
PubMed:
21855951
PubMed Central:
PMC3197739

9. Quantity and Quality of Free Oil Recovered from Enzymatically Disrupted Soybean Oleosomes

Author:
Murphy, Patricia A.,   et al.   ; Towa, Lili T.; Kapchie, Virginie N.; Wang, Guang; Hauck, Catherine; Wang, Tong;     Show all 6 Authors
Source:
journal of the American Oil Chemists' Society 2011 v.88 no.10 pp. 1581-1591
ISSN:
0003-021X
Subject:
fatty acid composition; free fatty acids; hexane; hydrolysis; lipid bodies; oils; oxidative stability; petroleum; slurries; solvents; soybeans; storage proteins
Abstract:
... The operational variables impacting the quantity and quality of free oil recovered from isolated soybean oleosomes by enzymatic extraction were evaluated to optimize this process. Those variables were: protease concentration (0–2.5%), protease hydrolysis time (3 vs. 18 h), and slurry destabilization time (30 min vs. 3 h). Analysis of interactions between these variables and the yield of free ...
DOI:
10.1007/s11746-011-1817-9

10. Pilot Plant Recovery of Soybean Oleosome Fractions by an Enzyme-Assisted Aqueous Process

Author:
Murphy, Patricia A.,   et al.   ; Towa, Lili T.; Kapchie, Virginie N.; Hauck, Catherine; Wang, Hui;     Show all 5 Authors
Source:
journal of the American Oil Chemists' Society 2011 v.88 no.5 pp. 733-741
ISSN:
0003-021X
Subject:
centrifugation; electron microscopy; enzymatic hydrolysis; equipment; fractionation; gel electrophoresis; grinding; lipid bodies; protein content; proximate composition; soy flour; soybeans
Abstract:
... An aqueous enzymatic procedure for oleosome fractionation from 25 g of soy flour was developed in our laboratory. This fractionation procedure was evaluated with 75 kg using pilot plant equipment to evaluate the effect of the scale-up on the recovery, proximate composition, soybean storage protein profiles, and subcellular microstructure of oleosome fractions. The process included enzymatic hydrol ...
DOI:
10.1007/s11746-010-1716-5

11. Efficacy of a Mycotoxin Binder against Dietary Fumonisin, Deoxynivalenol, and Zearalenone in Rats

Author:
Murphy, Patricia A.,   et al.   ; Qiang, Zhiyi; Truong, My; Meynen, Koen; Hendrich, Suzanne;     Show all 5 Authors
Source:
Journal of agricultural and food chemistry 2011 v.59 no.13 pp. 7527–7533
ISSN:
0021-8561
Subject:
adverse effects; alanine transaminase; deoxynivalenol; food intake; fumonisin B1; rats; sphingosine; weight gain; zearalenone
Abstract:
... It was hypothesized that a mycotoxin binder, Grainsure E, would inhibit adverse effects of a mixture of fumonisin B1, deoxynivalenol, and zearalenone in rats. For 14 and 28 days, 8–10 Sprague–Dawley rats were fed control diet, Grainsure E (0.5%), toxins (7 μg fumonisin B1/g, 8 μg of deoxynivalenol/g and 0.2 μg of zearalenone/g), toxins (12 μg of fumonisin B1/g, 9 μg of deoxynivalenol/g, and 0.2 μg ...
DOI:
10.1021/jf202042z
PubMed:
21650453

12. Bauer ketones 23 and 24 from Echinacea paradoxa var. paradoxa inhibit lipopolysaccharide-induced nitric oxide, prostaglandin E2 and cytokines in RAW264.7 mouse macrophages

Author:
Murphy, Patricia A.,   et al.   ; Zhang, Xiaozhu; Rizshsky, Ludmila; Hauck, Catherine; Qu, Luping; Widrlechner, Mark P.; Nikolau, Basil J.; Birt, Diane F.;     Show all 8 Authors
Source:
Phytochemistry 2012 v.74 pp. 146-158
ISSN:
0031-9422
Subject:
Echinacea; anti-inflammatory activity; common cold; cytokines; dimethyl sulfoxide; ethanol; fractionation; influenza; interleukin-1; interleukin-6; ketones; macrophages; mice; nitric oxide; polyacetylenes; prostaglandins
Abstract:
... Among the nine Echinacea species, E. purpurea, E. angustifolia and E. pallida, have been widely used to treat the common cold, flu and other infections. In this study, ethanol extracts of these three Echinacea species and E. paradoxa, including its typical variety, E. paradoxa var. paradoxa, were screened in lipopolysaccharide (LPS)-stimulated macrophage cells to assess potential anti-inflammatory ...
DOI:
10.1016/j.phytochem.2011.10.013
PubMed:
22133644
PubMed Central:
PMC3262908

13. Enzyme-Assisted Aqueous Extraction of Oil from Isolated Oleosomes of Soybean Flour

Author:
Murphy, Patricia A.,   et al.   ; Towa, Lili T.; Kapchie, Virginie N.; Hauck, Catherine;     Show all 4 Authors
Source:
journal of the American Oil Chemists' Society 2010 v.87 no.3 pp. 347-354
ISSN:
0003-021X
Subject:
soy flour; lipid bodies; soybean oil; enzymatic hydrolysis; serine proteinases; phospholipase A2; hydrolysates; plant proteins; subtilisin
Abstract:
... Enzyme-assisted aqueous extraction of oil from isolated soybean oleosomes was evaluated as an alternative to the conventional organic solvent extraction. Three different processes: hydrolysis of oleosomes, thermal demulsification of the skim or the slurry, and destabilization of the cream by the churning butter process were examined to enhance the release of free oil from isolated oleosomes. The o ...
DOI:
10.1007/s11746-009-1503-3

14. Recycling of Aqueous Supernatants in Soybean Oleosome Isolation

Author:
Murphy, Patricia A.,   et al.   ; Kapchie, Virginie N.; Towa, Lili T.; Hauck, Catherine;     Show all 4 Authors
Source:
journal of the American Oil Chemists' Society 2010 v.87 no.2 pp. 223-231
ISSN:
0003-021X
Subject:
soy flour; recycling; lipid bodies; chemical composition; peptides; proteinase inhibitors; glycinin
Abstract:
... Oleosome extractions from soybean flour typically generate significant quantities of aqueous sucrose- and sodium chloride-rich supernatant which could be recycled. To determine the feasibility of recycling the oleosome process aqueous supernatants, three extraction protocols were evaluated. The first extraction used the original extraction solution, 0.1 M fresh potassium acetate pH 4.6 containing ...
DOI:
10.1007/s11746-009-1485-1

15. Evaluation of enzyme efficiency for soy oleosome isolation and ultrastructural aspects

Author:
Murphy, Patricia A.,   et al.   ; Kapchie, Virginie N.; Towa, Lili T.; Hauck, Catherine;     Show all 4 Authors
Source:
Food research international 2010 v.43 no.1 pp. 241-247
ISSN:
0963-9969
Subject:
soy flour; lipid bodies; isolation; ultrastructure; enzymatic treatment; cellulases; polygalacturonase; crude protein; fractionation; beta-conglycinin; polyacrylamide gel electrophoresis; proximate composition
Abstract:
... Two procedures and four commercial enzymes were compared to isolate intact oleosomes from dry soybean flour. Results showed that Multifect® Pectinase FE efficiently liberated intact oleosomes in both procedures. The increase in oleosome extraction yield and reducing sugar production indicates that there was a synergistic effect on degrading the cell wall when theses enzymes were used together. In ...
DOI:
10.1016/j.foodres.2009.09.019

16. Preparation of Glycinin and β-Conglycinin from High-Sucrose/Low-Stachyose Soybeans

Author:
Murphy, Patricia A.,   et al.   ; Deak, Nicolas A.; Johnson, Lawrence A.;     Show all 3 Authors
Source:
journal of the American Oil Chemists' Society 2007 v.84 no.3 pp. 269-279
ISSN:
0003-021X
Subject:
soybeans; chemical composition; protein composition; thermal properties; hydrophobicity; emulsifying; glycinin; beta-conglycinin
Abstract:
... A new soybean line, known as high-sucrose/low-stachyose (HS/LS) soybeans, has been developed having elevated sucrose content and reduced content of flatus-causing oligosaccharides, especially stachyose. There is also increased interest in understanding the health benefits, functional properties and potential applications for the two major storage proteins of soybeans, glycinin and β-conglycinin. W ...
DOI:
10.1007/s11746-006-1031-3

17. Characterization of Fractionated Soy Proteins Produced by a New Simplified Procedure

Author:
Murphy, Patricia A.,   et al.   ; Deak, Nicolas A.; Johnson, Lawrence A.;     Show all 3 Authors
Source:
journal of the American Oil Chemists' Society 2007 v.84 no.2 pp. 137-149
ISSN:
0003-021X
Subject:
soybeans; soy protein; glycinin; beta-conglycinin
Abstract:
... It was possible to fractionate soy protein into two soy protein isolate fractions (>90% protein) enriched in either glycinin or β-conglycinin by using a new simplified procedure (referred to as the Deak procedure) employing CaCl₂ and NaHSO₃. The Deak procedure produced fractions with higher yields of solids, protein, and isoflavones, and similar protein purities as well as improved functional prop ...
DOI:
10.1007/s11746-006-1017-1

18. Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extracts across Caco-2 cell monolayers

Author:
Murphy, Patricia A.,   et al.   ; Qiang, Zhiyi; Ye, Zhong; Hauck, Cathy; McCoy, Joe-Ann; Widrlechner, Mark P.; Reddy, Manju B.; Hendrich, Suzanne;     Show all 8 Authors
Source:
Journal of ethnopharmacology 2011 v.137 no.3 pp. 1107-1112
ISSN:
0378-8741
Subject:
Prunella vulgaris; Salvia officinalis; bioavailability; biotransformation; epithelial cells; high performance liquid chromatography; humans; permeability; prediction; rosmarinic acid; sage; ursolic acid
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Rosmarinic acid (RA), a caffeic acid-related compound found in high concentrations in Prunella vulgaris (self-heal), and ursolic acid (UA), a pentacyclic triterpene acid concentrated in Salvia officinalis (sage), have been traditionally used to treat inflammation in the mouth, and may also be beneficial for gastrointestinal health in general. AIM OF THE STUDY: To in ...
DOI:
10.1016/j.jep.2011.07.037
PubMed:
21798330
PubMed Central:
PMC3202029

19. Glucuronides Are the Main Isoflavone Metabolites in Women

Author:
Murphy, Patricia A.,   et al.   ; Zhang, Yan; Hendrich, Suzanne;     Show all 3 Authors
Source:
Journal of nutrition 2003 v.133 no.2 pp. 399-404
ISSN:
0022-3166
Subject:
beta-glucuronidase; daidzein; genistein; in vitro studies; metabolism; metabolites; soymilk; urine; women
Abstract:
... Three experiments were conducted to characterize the metabolism of isoflavones from soy milk in women: two meals in 2 wk separated by a 1-wk washout period (Experiment 1), one meal feeding (Experiment 2) and six consecutive days of feeding (Experiment 3). Urine and plasma samples were extracted directly or predigested before extraction with H-2 β-glucuronidase/sulfatase or B-3 β-glucuronidase so t ...
PubMed:
12566474

20. Integrating Human Health and Environmental Health into the DPSIR Framework: A Tool to Identify Research Opportunities for Sustainable and Healthy Communities

Author:
Murphy, Patricia A.,   et al.   ; Yee, Susan H.; Bradley, Patricia; Fisher, William S.; Perreault, Sally D.; Quackenboss, James; Johnson, Eric D.; Bousquin, Justin;     Show all 8 Authors
Source:
Ecohealth 2012 v.9 no.4 pp. 411-426
ISSN:
1612-9202
Subject:
research planning; economic sustainability; business enterprises; bioinformatics; models; guidelines; human health; environmental health; asthma; scientists; United States
Abstract:
... The U.S. Environmental Protection Agency has recently realigned its research enterprise around the concept of sustainability. Scientists from across multiple disciplines have a role to play in contributing the information, methods, and tools needed to more fully understand the long-term impacts of decisions on the social and economic sustainability of communities. Success will depend on a shift in ...
DOI:
10.1007/s10393-012-0805-3
PubMed:
23238563