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1. An analysis of two open reading frames (ORF3 and ORF4) of rat hepatitis E virus genome using its infectious cDNA clones with mutations in ORF3 or ORF4

Author:
Nagashima, Shigeo,   et al.   ; Tanggis,; Kobayashi, Tominari; Takahashi, Masaharu; Jirintai, Suljid; Nishizawa, Tsutomu; Nishiyama, Takashi; Kunita, Satoshi; Hayama, Emiko; Tanaka, Takeshi; Mulyanto,; Okamoto, Hiroaki;     Show all 12 Authors
Source:
Virus research 2018 v.249 pp. 16-30
ISSN:
0168-1702
Subject:
Orthohepevirus A; messenger RNA; leucine; liver; protein synthesis; complementary DNA; open reading frames; genome; proline; mutation; clones; mutants; virus replication; rats; tissues
Abstract:
... Rat hepatitis E virus (ratHEV) genome has four open reading frames (ORFs: ORF1, ORF2, ORF3 and ORF4). The functions of ORF3 and ORF4 are unknown. An infectious cDNA clone (pUC-ratELOMB-131L_wt, wt) and its derivatives including ORF3-defective (ΔORF3) and ORF4-defective (ΔORF4) mutants, were constructed and their full-length RNA transcripts transfected into PLC/PRF/5 cells. ΔORF3 replicated as effi ...
DOI:
10.1016/j.virusres.2018.02.014

2. Production of monoclonal antibodies against the ORF3 protein of rat hepatitis E virus (HEV) and demonstration of the incorporation of the ORF3 protein into enveloped rat HEV particles

Author:
Nagashima, Shigeo,   et al.   ; Takahashi, Masaharu; Kobayashi, Tominari; Tanggis,; Jirintai, Suljid; Mulyanto,; Nishizawa, Tsutomu; Kunita, Satoshi; Okamoto, Hiroaki;     Show all 9 Authors
Source:
Archives of virology 2016 v.161 no.12 pp. 3391-3404
ISSN:
0304-8608
Subject:
Orthohepevirus A; trypsin; surface proteins; blood serum; humans; virion; sucrose; feces; mice; amino acids; monoclonal antibodies; cytoplasm; deoxycholic acid; plasmids; rats; fluorescent antibody technique; synthetic peptides; detergents
Abstract:
... Eight murine monoclonal antibodies (MAbs) against a synthetic peptide corresponding to the C-terminal 15-amino-acid portion of the ORF3 protein of rat hepatitis E virus (ratHEV) were produced and characterized. Immunofluorescence assays using the anti-ratHEV ORF3 MAbs revealed the accumulation of ORF3 protein in the cytoplasm of PLC/PRF/5 cells transfected with ORF3-expressing plasmids or inoculat ...
DOI:
10.1007/s00705-016-3047-9

3. Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone

Author:
Nagashima, Shigeo,   et al.   ; Kobayashi, Tominari; Tanaka, Toshinori; Tanggis,; Jirintai, Suljid; Takahashi, Masaharu; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 8 Authors
Source:
Virus research 2016 v.223 pp. 170-180
ISSN:
0168-1702
Subject:
Orthohepevirus A; proteins; complementary DNA; cell culture; mutation; clones; viruses; genotype; nucleotide sequences; progeny; viral load; fluorescent antibody technique
Abstract:
... To characterize the genomic mutations of hepatitis E virus (HEV) during consecutive passages associated with adaptation to growth in cell culture, a cloned genotype 3 HEV [pJE03-1760F/wt, starting virus (SV)] was passaged 10 times in A549 cells, and the entire genomic sequence of the passage 10 (P10) progeny was determined. Compared to SV, P10 virus possessed two non-synonymous (T2808C and A5054G) ...
DOI:
10.1016/j.virusres.2016.07.011

4. Characterization and epitope mapping of monoclonal antibodies raised against rat hepatitis E virus capsid protein: An evaluation of their neutralizing activity in a cell culture system

Author:
Nagashima, Shigeo,   et al.   ; Kobayashi, Tominari; Takahashi, Masaharu; Tanggis,; Mulyanto,; Jirintai, Suljid; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 8 Authors
Source:
Journal of virological methods 2016 v.233 pp. 78-88
ISSN:
0166-0934
Subject:
coat proteins; Orthohepevirus A; cross reaction; neutralization; trypsin; epitope mapping; hepatitis; humans; capsid; cell culture; lipids; mice; amino acids; monoclonal antibodies; viruses; deoxycholic acid; rats; virus replication
Abstract:
... Hepatitis E virus (HEV) is the causative agent of acute hepatitis. Rat HEV is a recently discovered virus related to, but distinct from, human HEV. Since laboratory rats can be reproducibly infected with rat HEV and a cell culture system has been established for rat HEV, this virus may be used as a surrogate virus for human HEV, enabling studies on virus replication and mechanism of infection. How ...
DOI:
10.1016/j.jviromet.2016.03.004

5. Full-length genomic sequences of new subtype 1g hepatitis E virus strains obtained from four patients with imported or autochthonous acute hepatitis E in Japan

Author:
Nagashima, Shigeo,   et al.   ; Nishizawa, Tsutomu; Primadharsini, Putu Prathiwi; Namikawa, Masashi; Yamazaki, Yuichi; Uraki, Satoko; Okano, Hiroshi; Horiike, Shinichiro; Nakano, Tatsunori; Takaki, Shintaro; Kawakami, Manri; Takahashi, Masaharu; Okamoto, Hiroaki;     Show all 13 Authors
Source:
Infection, genetics, and evolution 2017 v.55 pp. 343-349
ISSN:
1567-1348
Subject:
Orthohepevirus A; geographical distribution; humans; hepatitis E; genome; nucleotides; phylogeny; genotype; chronic hepatitis; nucleotide sequences; patients; France; Israel; Netherlands; India; Japan; Pakistan; Portugal; United Kingdom
Abstract:
... Hepatitis E virus (HEV) causes acute or chronic hepatitis in humans worldwide and can be transmitted via the fecal-oral route. Four HEV strains (HE-JA14-2173, HE-JA15-1335, HE-JA15-1920 and HE-JA16-0610) obtained from patients with imported (from Pakistan or India) or autochthonous acute hepatitis E in Japan were most closely related to the Nepalese and Mongolian genotype 1 HEV strains of unassign ...
DOI:
10.1016/j.meegid.2017.10.007

6. Full-length genome of a novel genotype 3 hepatitis E virus strain obtained from domestic pigs in Japan

Author:
Nagashima, Shigeo,   et al.   ; Primadharsini, Putu Prathiwi; Miyake, Masao; Kunita, Satoshi; Nishizawa, Tsutomu; Takahashi, Masaharu; Tanggis,; Ohnishi, Hiroshi; Kobayashi, Tominari; Nishiyama, Takashi; Jirintai, Suljid; Okamoto, Hiroaki;     Show all 12 Authors
Source:
Virus research 2017 v.240 pp. 147-153
ISSN:
0168-1702
Subject:
Orthohepevirus A; immunoglobulin G; humans; herds; antibodies; genome; genotype; chronic hepatitis; nucleotide sequences; swine; Japan
Abstract:
... Hepatitis E virus (HEV) causes acute or chronic hepatitis in humans and can be transmitted via the fecal-oral route. Pigs are one of the main reservoirs for this infection. Sixty pigs, 4–5 months of age, on a swine herd in Japan had detectable anti-HEV IgG antibodies, and five (8.3%) of them had ongoing infection of genotype 3 HEV. Five HEV strains obtained from the viremic pigs shared 98.8–100% n ...
DOI:
10.1016/j.virusres.2017.08.003

7. The membrane on the surface of hepatitis E virus particles is derived from the intracellular membrane and contains trans-Golgi network protein 2

Author:
Nagashima, Shigeo,   et al.   ; Takahashi, Masaharu; Jirintai, Suljid; Tanggis,; Kobayashi, Tominari; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 7 Authors
Source:
Archives of virology 2014 v.159 no.5 pp. 979-991
ISSN:
0304-8608
Subject:
digitonin; Orthohepevirus A; antigens; reverse transcriptase polymerase chain reaction; mice; viruses; cytoplasm; surface proteins; fluorescent antibody technique
Abstract:
... Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclonal antibody (mAb) against the membrane-associated HEV particles to examine wheth ...
DOI:
10.1007/s00705-013-1912-3
PubMed:
24221250

8. Rat hepatitis E virus derived from wild rats (Rattus rattus) propagates efficiently in human hepatoma cell lines

Author:
Nagashima, Shigeo,   et al.   ; Jirintai, Suljid; Tanggis,; Mulyanto,; Suparyatmo, Joseph Benedictus; Takahashi, Masaharu; Kobayashi, Tominari; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 9 Authors
Source:
Virus research 2014 v.185 pp. 92-102
ISSN:
0168-1702
Subject:
Orthohepevirus A; proteins; human cell lines; rats; liver; hepatoma; humans; zoonoses; antibodies; cell culture; proteinases; sucrose; genomics; detergents; RNA; Northern blotting; virus replication; Rattus rattus; Indonesia
Abstract:
... Although rat hepatitis E virus (HEV) has been identified in wild rats, no cell culture systems for this virus have been established. A recent report suggesting the presence of antibodies against rat HEV in human sera encouraged us to cultivate rat HEV in human cells. When liver homogenates obtained from wild rats (Rattus rattus) in Indonesia were inoculated onto human hepatocarcinoma cells, the ra ...
DOI:
10.1016/j.virusres.2014.03.002

9. Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia

Author:
Nagashima, Shigeo,   et al.   ; Mulyanto,; Depamede, Sulaiman Ngongu; Sriasih, Made; Takahashi, Masaharu; Jirintai, Suljid; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 8 Authors
Source:
Archives of virology 2013 v.158 no.1 pp. 87-96
ISSN:
0304-8608
Subject:
Orthohepevirus A; sequence analysis; immunoglobulin G; antibodies; genome; enzyme-linked immunosorbent assay; genotype; nucleotide sequences; population density; rats; RNA; Rattus rattus; Indonesia
Abstract:
... One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotyp ...
DOI:
10.1007/s00705-012-1462-0
PubMed:
22983110

10. Molecular and serological survey of hepatitis E virus infection among domestic pigs in Inner Mongolia, China

Author:
Nagashima, Shigeo,   et al.   ; Jinshan; Jirintai; Manglai, Dugarjavin; Takahashi, Masaharu; Okamoto, Hiroaki;     Show all 6 Authors
Source:
Archives of virology 2010 v.155 no.8 pp. 1217-1226
ISSN:
0304-8608
Subject:
Orthohepevirus A; serological surveys; humans; antibodies; farms; genotype; RNA; swine; Mongolia; China
Abstract:
... To evaluate the prevalence and characteristics of swine hepatitis E virus (HEV) infection in Inner Mongolia, China, serum samples obtained from 356 2- to 4-month-old pigs on 14 farms in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 186 pigs (52%) tested positive for anti-HEV antibodies, while 30 pigs (8%) had detectable HEV RNA levels. The 30 HEV isolates ...
DOI:
10.1007/s00705-010-0706-0
PubMed:
20512514

11. Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains

Author:
Nagashima, Shigeo,   et al.   ; Takahashi, Masaharu; Nishizawa, Tsutomu; Jirintai, Suljid; Kawakami, Manri; Sonoda, Yoshihide; Suzuki, Tadahiro; Yamamoto, Shogo; Shigemoto, Kazuhiro; Ashida, Kozo; Sato, Yukihiro; Okamoto, Hiroaki;     Show all 12 Authors
Source:
Virus research 2014 v.180 pp. 59-69
ISSN:
0168-1702
Subject:
rabbits; Orthohepevirus A; boars; humans; genome; Herpestidae; nucleotides; phylogeny; Sus scrofa; Chiroptera; ferrets; surveys; wild boars; genotype; genomics; rats; deer; birds; Japan
Abstract:
... Although a consensus classification system for hepatitis E virus (HEV) genotypes is currently unavailable, HEV variants (JBOAR135-Shiz09 and wbJOY_06) from wild boars (Sus scrofa leucomystax) have provisionally been classified into two novel genotypes (5 and 6). While performing a survey of HEV infections among 566 wild boars that were captured in Japan between January 2010 and August 2013, we fou ...
DOI:
10.1016/j.virusres.2013.12.014

12. Marked genomic heterogeneity of rat hepatitis E virus strains in Indonesia demonstrated on a full-length genome analysis

Author:
Nagashima, Shigeo,   et al.   ; Mulyanto,; Suparyatmo, Joseph Benedictus; Andayani, I Gusti Ayu Sri; Khalid,; Takahashi, Masaharu; Ohnishi, Hiroshi; Jirintai, Suljid; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 10 Authors
Source:
Virus research 2014 v.179 pp. 102-112
ISSN:
0168-1702
Subject:
Orthohepevirus A; sequence analysis; genome; antibodies; reverse transcriptase polymerase chain reaction; genomics; nucleotide sequences; rats; RNA; polyphyly; Rattus rattus; Germany; Vietnam; Indonesia
Abstract:
... Rat hepatitis E virus (HEV) strains have recently been isolated in several areas of Germany, Vietnam, the United States, Indonesia and China. However, genetic information regarding these rat HEV strains is limited. A total of 369 wild rats (Rattus rattus) captured in Central Java (Solo) and on Lombok Island, Indonesia were tested for the presence of rat HEV-specific antibodies and RNA. Overall, 13 ...
DOI:
10.1016/j.virusres.2013.10.029

13. Full genomic analyses of human rotavirus strains possessing the rare P[8]b VP4 subtype

Author:
Nagashima, Shigeo,   et al.   ; Ghosh, Souvik; Paul, Shyamal Kumar; Yamamoto, Dai; Kobayashi, Nobumichi;     Show all 5 Authors
Source:
Infection, genetics, and evolution 2011 v.11 no.6 pp. 1481-1486
ISSN:
1567-1348
Subject:
Rotavirus B; diarrhea; genes; genetic relationships; genomics; genotype; humans; nucleotide sequences; phylogeny; public health; vaccines; Bangladesh
Abstract:
... Rotaviruses with the P[8] VP4 genotype are a major cause of acute infantile diarrhea. The P[8] genotype is classified into two genetically distinct subtypes, P[8]a and P[8]b. Most of the P[8] strains belong to subtype P[8]a, whilst P[8]b strains are rare. To date, the whole genomes of a few P[8]a strains have been analyzed, whilst there are no reports on full genomic analysis of the P[8]b strains. ...
DOI:
10.1016/j.meegid.2011.05.014
PubMed:
21640848

14. Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells

Author:
Nagashima, Shigeo,   et al.   ; Jirintai, Suljid; Jinshan,; Tanggis,; Manglai, Dugarjavin; Mulyanto,; Takahashi, Masaharu; Kobayashi, Tominari; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 10 Authors
Source:
Virus research 2012 v.170 no.1-2 pp. 126-137
ISSN:
0168-1702
Subject:
rabbits; Orthohepevirus A; host range; human cell lines; risk; liver; antibodies; farms; phylogeny; nucleotide sequences; RNA; France; China
Abstract:
... Rabbit hepatitis E virus (HEV) strains have recently been isolated in several areas of China and in the US and France. However, the host range, distribution and zoonotic potential of these HEV strains remain unknown and their propagation in cultured cells has not yet been reported. A total of 211 4-month-old rabbits raised on a farm in Inner Mongolia were tested for the presence of anti-HEV antibo ...
DOI:
10.1016/j.virusres.2012.09.015

15. A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food

Author:
Nagashima, Shigeo,   et al.   ; Takahashi, Hideyuki; Tanaka, Toshinori; Jirintai, Suljid; Takahashi, Masaharu; Nishizawa, Tsutomu; Mizuo, Hitoshi; Yazaki, Yasuyuki; Okamoto, Hiroaki;     Show all 9 Authors
Source:
Archives of virology 2012 v.157 no.2 pp. 235-246
ISSN:
0304-8608
Subject:
Orthohepevirus A; risk; foods; boars; liver; humans; food animals; cell culture; feces; pathogenicity; viruses; wild boars; progeny; culture media
Abstract:
... Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs and wild boars to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. However, no efficient cell culture system for swine and boar HEV strains has been established. We inoculated A549 cells with 12 swine and boar HEV strains of liver, feces, or serum origin ...
DOI:
10.1007/s00705-011-1153-2
PubMed:
22048607

16. Identification of four novel subgenotypes (C13–C16) and two inter-genotypic recombinants (C12/G and C13/B3) of hepatitis B virus in Papua Province, Indonesia

Author:
Nagashima, Shigeo,   et al.   ; Mulyanto,; Pancawardani, Pingki; Depamede, Sulaiman Ngongu; Wahyono, Arif; Jirintai, Suljid; Takahashi, Masaharu; Nishizawa, Tsutomu; Okamoto, Hiroaki;     Show all 9 Authors
Source:
Virus research 2012 v.163 no.1 pp. 129-140
ISSN:
0168-1702
Subject:
DNA; Hepatitis B virus; blood serum; genes; hybrids; nationalities and ethnic groups; nucleotide sequences; phylogeny; Indonesia
Abstract:
... Four novel subgenotypes (C6, C11, C12, and D6) of hepatitis B virus (HBV) were identified in Papua, a multiethnic province of Indonesia. To characterize the HBV strains in Papua, serum samples collected from 515 indigenous inhabitants (mean age: 26.6±9.6years) in a previously unexamined area, Nabire, located in northern Papua, were used in the present study. Among 46 samples whose 1.6-kilobase (kb ...
DOI:
10.1016/j.virusres.2011.09.002

17. Identification and characterization of a natural inter-genotypic (2b/1b) recombinant hepatitis C virus in Japan

Author:
Nagashima, Shigeo,   et al.   ; Yokoyama, Koji; Takahashi, Masaharu; Nishizawa, Tsutomu; Jirintai, Suljid; Yotsumoto, Shigeru; Okamoto, Hiroaki; Momoi, Mariko Y.;     Show all 8 Authors
Source:
Archives of virology 2011 v.156 no.9 pp. 1591-1601
ISSN:
0304-8608
Subject:
Hepatitis C virus; amino acids; chronic hepatitis C; females; genotype; nucleotides; open reading frames; Japan; Philippines
Abstract:
... A hepatitis C virus (HCV) strain (HC10-0804) recovered from a 12-year-old Japanese female with chronic hepatitis C segregated into discordant genotypes, 2b and 1b, in the 5’UTR/core and NS5B regions, respectively, thus suggesting an inter-genotypic recombination. The HC10-0804 isolate had a genomic length of 9,423 nucleotides (nt), excluding the poly(U) tract at the 3’ terminus, and encoded a sing ...
DOI:
10.1007/s00705-011-1038-4
PubMed:
21643787

18. Characterization of full-length VP4 genes of OP354-like P[8] human rotavirus strains detected in Bangladesh representing a novel P[8] subtype

Author:
Nagashima, Shigeo,   et al.   ; Kobayashi, Nobumichi; Paul, Shyamal Kumar; Alam, Mohammed Mahbub; Chawla-Sarkar, Mamta; Krishnan, Triveni;     Show all 6 Authors
Source:
Archives of virology 2009 v.154 no.8 pp. 1223-1231
ISSN:
0304-8608
Subject:
genes; genotype; nucleotide sequences; phylogeny; sequence analysis; Bangladesh
Abstract:
... The G1 and G9 rotavirus strains MMC71 and MMC38 (subgroup II, NSP4 genogroup B), respectively, isolated from children in Bangladesh, were analyzed genetically. Full-length VP4 genes of these strains had 98.9% identity to each other and showed 83.9-89.4% identity to those of the P[4] and P[8] rotaviruses. Phylogenetic analysis of VP4 nucleotide sequences revealed that strains MMC38 and MMC71 were l ...
DOI:
10.1007/s00705-009-0436-3
PubMed:
19572186

19. Determination of the 5′-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells

Author:
Nagashima, Shigeo,   et al.   ; Ichiyama, Koji; Yamada, Kentaro; Tanaka, Toshinori; Jirintai; Takahashi, Masaharu; Okamoto, Hiroaki;     Show all 7 Authors
Source:
Archives of virology 2009 v.154 no.12 pp. 1945-1951
ISSN:
0304-8608
Subject:
Orthohepevirus A; complementary DNA; mutation; clones; cultured cells; genotype; RNA
Abstract:
... Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5′-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the ge ...
DOI:
10.1007/s00705-009-0538-y
PubMed:
19885718

20. Identification and characterization of novel hepatitis B virus subgenotype C10 in Nusa Tenggara, Indonesia

Author:
Nagashima, Shigeo,   et al.   ; Mulyanto; Depamede, Sulaiman Ngongu; Surayah, Kiely; Tjahyono, Andreas Andhry Heru; Jirintai; Takahashi, Masaharu; Okamoto, Hiroaki;     Show all 8 Authors
Source:
Archives of virology 2010 v.155 no.5 pp. 705-715
ISSN:
0304-8608
Subject:
Hepatitis B virus; blood; genome; genotype; phylogeny; Indonesia
Abstract:
... Six novel subgenotypes (B7, B8, C6, C8, C9, and D6) within three hepatitis B virus (HBV) genotypes (B-D) were recently identified in Indonesia. To further characterize HBV in this country, 18 HBV-viremic samples obtained from blood donors in Nusa Tenggara, Indonesia, were subjected to phylogenetic analysis of an 1.6-kb partial or full-length sequence. Thirteen HBV isolates were classified into gen ...
DOI:
10.1007/s00705-010-0628-x
PubMed:
20306210