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- Nol, Chea, et al. Show all 6 Authors
- Applied and environmental microbiology 2011 v.77 no.13 pp. 4336-4343
- Norovirus; RNA; aquatic environment; complementary DNA; mice; reverse transcriptase polymerase chain reaction; silica; virion; viruses
- ... Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing nucleic acid yields and DNA amplification efficiencies for environmental water samples. This involved adding particles of adeno ...