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1. Anti‐PirA‐like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease

Author:
Nozaki, Reiko,   et al.   ; Nakamura, Rika; Pedrosa‐Gerasmio, Ivane R.; Alenton, Rod Russel R.; Kondo, Hidehiro; Hirono, Ikuo;     Show all 6 Authors
Source:
Journal of fish diseases 2019 v.42 no.8 pp. 1125-1132
ISSN:
0140-7775
Subject:
Litopenaeus vannamei; Vibrio parahaemolyticus; antibiotics; antigens; diet; egg yolk; eggs; feeds; hens; immunization; immunoglobulin Y; necrotizing hepatopancreatitis; powders; protective effect; shrimp; shrimp culture; survival rate; toxins
Abstract:
... Acute hepatopancreatic necrosis disease (AHPND), caused by a toxin‐producing Vibrio parahaemolyticus strain, has become a serious threat to shrimp aquaculture. The need to regulate antibiotic use prompted the development of alternative ways to treat infections in aquaculture including the use of chicken egg yolk immunoglobulin (IgY) for passive immunization. This study evaluated the protective eff ...
DOI:
10.1111/jfd.13024

2. LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus

Author:
Nozaki, Reiko,   et al.   ; Rondón-Barragán, Iang; Hirono, Ikuo; Kondo, Hidehiro;     Show all 4 Authors
Source:
Fish & shellfish immunology 2017 v.67 pp. 546-553
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Iridovirus; Pagrus major; Paralichthys olivaceus; antibodies; antigens; complementary DNA; cytoplasmic granules; farmed fish; flounder; fluorescent antibody technique; gene expression; genes; recombinant vaccines; spleen; vaccination
Abstract:
... DNA vaccination is one method to protect farmed fish from viral and bacterial diseases. Chimeric antigens encoded by DNA vaccines have been shown to increase the resistance to viral diseases. Here, we sequenced the gene encoding lysosome-associated membrane protein-1 from Japanese flounder, Paralichthys olivaceus, (JfLAMP-1) and assessed its use in a chimeric DNA vaccine fused with the major capsu ...
DOI:
10.1016/j.fsi.2017.06.045

3. Development of 11 Ecklonia radicosa (Phaeophyceae, Laminariales) SSRs markers using next-generation sequencing and intra-genus amplification analysis

Author:
Nozaki, Reiko,   et al.   ; Akita, Shingo; Koiwai, Keiichiro; Hanyuda, Takeaki; Kato, Syou; Uchino, Tsubasa; Sakamoto, Takashi; Kondo, Hidehiro; Hirono, Ikuo; Fujita, Daisuke;     Show all 10 Authors
Source:
Journal of applied phycology 2018 v.30 no.3 pp. 2111-2115
ISSN:
0921-8971
Subject:
DNA; Ecklonia; alleles; genetic variation; heterozygosity; high-throughput nucleotide sequencing; loci; microsatellite repeats; population structure
Abstract:
... Polymorphic simple sequence repeat (SSR) markers of Ecklonia radicosa were developed using Illumina MiSeq next-generation sequencing (NGS) analysis. In total, 3112 SSRs (di- to hexanucleotide motifs repeated more than six times) were identified from 61.5-Mb assembled genomic DNA using MISA perl script. Among the SSRs, di- (853 loci, 27.4%) and trinucleotides (1813 loci, 58.3%) were dominant, while ...
DOI:
10.1007/s10811-018-1406-5

4. Two hemocyte sub-populations of kuruma shrimp Marsupenaeus japonicus

Author:
Nozaki, Reiko,   et al.   ; Koiwai, Keiichiro; Alenton, Rod Russel R.; Shiomi, Reina; Kondo, Hidehiro; Hirono, Ikuo;     Show all 6 Authors
Source:
Molecular Immunology 2017 v.85 pp. 1-8
ISSN:
0161-5890
Subject:
Marsupenaeus japonicus; centrifugation; crustin; flow cytometry; gene expression; gene expression regulation; genes; genetic markers; granules; hemocytes; immune response; immune system; pathogens; sequence analysis; shrimp; staining; transcriptomics
Abstract:
... Hemocytes in the circulating hemolymph play important roles for immune responses in shrimp. Previous studies on immune responses by hemocytes in penaeid shrimp were based on gene expression analyses of the entire population of hemocytes and thus may have missed different immune responses of different hemocyte sub-populations. In this study, we separated hemocytes into two sub-populations by Percol ...
DOI:
10.1016/j.molimm.2017.01.024

5. Cloning and expression analysis of three novel CC chemokine genes from Japanese flounder (Paralichthys olivaceus)

Author:
Nozaki, Reiko,   et al.   ; Zou, Gang-gang; Kondo, Hidehiro; Hirono, Ikuo;     Show all 4 Authors
Source:
Fish & shellfish immunology 2014 v.40 no.2 pp. 507-513
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Paralichthys olivaceus; Streptococcus iniae; Viral hemorrhagic septicemia virus; beta chemokines; flounder; gene expression; genes; immune response; kidneys; pathogens; spleen; tissues
Abstract:
... Chemokines are small cytokines secreted by various cell types. They not only function in cell activation, differentiation and trafficking, but they also have influences on many biological processes. In this study, three novel CC chemokine genes Paol-SCYA105, 106 and 107 in Japanese flounder (Paralichthys olivaceus) were cloned and characterized. Paol-SCYA105 was mainly detected in gill, kidney and ...
DOI:
10.1016/j.fsi.2014.08.003

6. Identification of novel interleukin 1 beta family genes in Japanese flounder Paralichthys olivaceus

Author:
Nozaki, Reiko,   et al.   ; Taechavasonyoo, Apichaya; Kondo, Hidehiro; Suzuki, Yutaka; Hirono, Ikuo;     Show all 5 Authors
Source:
Fish & shellfish immunology 2013 v.34 no.1 pp. 393-396
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Oncorhynchus mykiss; Oreochromis niloticus; Paralichthys olivaceus; Salvelinus alpinus; agonists; amino acid sequences; exons; flounder; genes; gills; inflammation; interleukin-1; intestines; kidneys; messenger RNA; open reading frames; phylogeny; spleen
Abstract:
... Gene in the interleukin 1 family plays a central role in the regulation of immune and inflammatory responses. Here, we describe two novel IL-1-like gene in Japanese flounder Paralichthys olivaceus (jfIL1-L1 and jfIL1-L2). jfIL1β-L1 was homologous to Nile Tilapia IL-1β-like gene and Arctic char IL-1, and jfIL1β-L2 showed homology to hypothetical protein LOC100699119 of Nile Tilapia and rainbow trou ...
DOI:
10.1016/j.fsi.2012.10.001

7. Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili

Author:
Nozaki, Reiko,   et al.   ; Jirapongpairoj, Walissara; Kobayashi, Keigo; Fukuda, Yutaka; Takano, Tomokazu; Sakai, Takamitsu; Matsuyama, Tomomasa; Nakayasu, Chihaya; Hirono, Ikuo; Kondo, Hidehiro;     Show all 10 Authors
Source:
Fisheries science 2015 v.81 no.5 pp. 907-914
ISSN:
0919-9268
Subject:
Seriola quinqueradiata; Vibrio anguillarum; gene expression regulation; genes; high-throughput nucleotide sequencing; interleukin-10; interleukin-1beta; interleukin-8; kidneys; pathogens; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; tumor necrosis factor-alpha; vaccination
Abstract:
... Partial sequences of cytokine genes IFNγ, IL-1β, IL-8, IL-10, and TNFα were identified from next-generation sequencing data for three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili. The cytokine genes had more than 90 % nucleotide identity among the three species. Quantitative PCR (qPCR) primers were designed from the consensus sequences to detect cytokine genes. The specifi ...
DOI:
10.1007/s12562-015-0913-4

8. Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicus

Author:
Nozaki, Reiko,   et al.   ; Hung, Mai Nam; Shiomi, Reina; Kondo, Hidehiro; Hirono, Ikuo;     Show all 5 Authors
Source:
Fish & shellfish immunology 2014 v.40 no.2 pp. 472-477
ISSN:
1050-4648
Subject:
Marsupenaeus japonicus; Vibrio penaeicida; amino acids; gene expression regulation; genes; gills; hemocytes; innate immunity; messenger RNA; phylogeny; shrimp; superoxide anion; superoxide dismutase; zinc
Abstract:
... Superoxide dismutases (SODs) protect cells from superoxides, but in invertebrates, they also have role in the innate immune system. In this study, the genes for five isoforms of copper/zinc superoxide dismutase (MjCu/ZnSOD) gene were identified and sequenced in kuruma shrimp, Marsupenaeus japonicus. The coding parts of the genes ranged from 516 to 585 bp in length and encoded from 172 to 194 amino ...
DOI:
10.1016/j.fsi.2014.07.030

9. EST analysis on adipose tissue of rainbow trout Oncorhynchus mykiss and tissue distribution of adiponectin

Author:
Nozaki, Reiko,   et al.   ; Kondo, Hidehiro; Suga, Ryota; Suda, Satomitsu; Hirono, Ikuo; Nagasaka, Reiko; Kaneko, Gen; Ushio, Hideki; Watabe, Shugo;     Show all 9 Authors
Source:
Gene 2011 v.485 no.1 pp. 40-45
ISSN:
0378-1119
Subject:
Oncorhynchus mykiss; adipocytes; adiponectin; adipose tissue; apolipoprotein C-I; complementary DNA; databases; digestive enzymes; energy metabolism; expressed sequence tags; fatty acid-binding proteins; fatty acids; fish; genes; glucose; lipid metabolism; mammals; muscles; oxidation; pancreas; reverse transcriptase polymerase chain reaction; tissue distribution
Abstract:
... Although energy metabolism in mammals is critically regulated by adipokines from adipocytes, it is unclear whether this is the case in fish as well. In this study, over 30,000 expressed sequence tags (ESTs) were obtained from adipose tissue in rainbow trout Oncorhynchus mykiss peritoneal cavity and searched for genes possibly related to lipid metabolism. Large numbers of ESTs encoded digestive enz ...
DOI:
10.1016/j.gene.2011.05.035
PubMed:
21712077