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- Orio, Lara P., et al. Show all 8 Authors
- Journal of agricultural and food chemistry 2017 v.65 no.48 pp. 10482-10488
- Cannabis sativa; acid deposition; hemp; hydrochloric acid; hydrolysates; hydrolysis; inhibitory concentration 50; mass spectrometry; nuclear magnetic resonance spectroscopy; peptides; peptidyl-dipeptidase A; protein isolates; reversed-phase high performance liquid chromatography; solubilization
- ... A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments a ...