An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... A novel chymotrypsin inhibitor, named ClCI, was purified from coix seed (Coix lacryma-jobi L.) by aqueous two-phase extraction, chymotrypsin–Sepharose 4B affinity chromatography and centrifugal ultrafiltration. ClCI was a 7.9 kDa competitive inhibitor with pI 6.54. The inhibition constants (Ki) for bovine pancreatic chymotrypsin and bacterial subtilisin were 1.27 × 10−10 M and 1.57 × 10−9 M respec ...
... Angular leaf spot (ALS), caused by the fungus Phaeoisariopsis griseola is an economically important and widely distributed disease of common bean. Due to the co-evolution of P. griseola with the large and small seeded bean gene pools, stacking Andean and Mesoamerican resistance genes is a strategy most likely to provide lasting resistance to ALS disease. This strategy requires identification and c ...
Phaeoisariopsisgriseola, etc ; pesticide application; cultural control; plowing; crop residues; fungicides; disease control; Phaseolus vulgaris; leaf spot; green beans; varieties; disease resistance; integrated pest management; crop rotation; Ontario; Show all 15 Subjects
Abstract:
... Angular leaf spot (ALS) caused by the fungus Phaeoisariopsis griseola (Sacc.) Ferr. was first observed and confirmed on snap beans growing in three commercial fields in southern Ontario during the 2000 growing season. The potential impact of this disease on the bean industry in Ontario is not known but this disease is severe in many other regions. The objective of this study was to develop a disea ...
Phaeoisariopsisgriseola, etc ; cultivars; beans; races; genetic resistance; fungal diseases of plants; Phaseolus vulgaris; leaf spot; genotype; disease resistance; plant pathogenic fungi; virulence; Malawi; Eastern Africa; Show all 14 Subjects
Abstract:
... Common beans (Phaseolus vulgaris) are separated into two distinct groups: Andean and Middle American. We identified CAL 143 as the first Andean bean with resistance to angular leaf spot disease caused by Phaeoisariopsis griseola. Angular leaf spot is the most widespread and economically important bean disease in southern and eastern Africa, and it is especially severe on the extensively grown Ande ...
Phaeoisariopsisgriseola, etc ; Phaseolus vulgaris; Xanthomonas campestris pv. phaseoli; beans; blight; dyes; genes; leaf spot; pesticide use reduction; Show all 9 Subjects
Abstract:
... Bean golden mosaic (BGM) resistance within any one bean (Phaseolus vulgaris L.) race is inadequate. Pyramiding genes from different races would increase resistance and reduce pesticide use. Our objectives were to: (i) pyramid and compare BGM resistance of genotypes from intraracial versus interracial populations, (ii) verify pyramided resistance by means of molecular markers, and (iii) combine BGM ...
Phaeoisariopsisgriseola, etc ; Phaseolus vulgaris; Uromyces appendiculatus; rust diseases; crop yield; field experimentation; leaf area; duration; defoliation; photosynthesis; mixed infection; Brazil; Show all 12 Subjects
Abstract:
... Three field experiments were conducted in 1997, 1998, and 1999 to investigate the effects of angular leaf spot and rust, separately or combined, on host growth and yield of individual bean plants (Phaseolus vulgaris). In each experiment, three treatments were established by inoculating cv. Carioca with Phaeoisariopsis griseola, Uromyces appendiculatus, or with both pathogens. An additional control ...
Phaeoisariopsisgriseola, etc ; plant breeding; beans; marker-assisted selection; random amplified polymorphic DNA technique; cultivars; fungal diseases of plants; Phaseolus vulgaris; leaf spot; plant pathogenic fungi; disease resistance; genes; genetic markers; Show all 13 Subjects
Abstract:
... The objective of this work was to develop homozygous common bean lines carrying angular leaf spot resistance genes derived from the cultivars 'Mexico 54', 'MAR 2' and 'BAT 332' through markerassisted selection. Molecular markers SCAR OPN02(890), RAPD OPE04(500) and OPAO12(950) linked to the resistance genes of 'Mexico 54', 'MAR 2' and 'BAT 332', respectively, were used in segregating backcross-der ...
Phaeoisariopsisgriseola, etc ; Escherichia coli; alleles; beans; fungi; genetic recombination; genomic libraries; leaf spot; microsatellite repeats; pathogens; plant protection; plasmids; Central America; Show all 13 Subjects
Abstract:
... Although several researches revealed an important diversity within Phaeoisariopsis griseola, the bean angular leaf spot (ALS) agent, no sexual recombination was already detected for this fungus. That apparent contradiction gave rise to the interest to develop codominant markers in order of a more precise analysis of the pathogen populations. Microsatellites were expected to allow characterising P. ...
... The yield of common bean (Phaseolus vulgaris L.) is affected by several factors, including diseases such as angular leaf spot (Phaeoisariopsis griseola) and N deficiency caused by lack of nodulation. In Minas Gerais State, Brazil, farmers have been using both fungicide and molybdenum (Mo) applications to improve crop yield. The objective of this research was to investigate the effect of Mo applica ...
Phaeoisariopsisgriseola, etc ; random amplified polymorphic DNA technique; conidia; geographical variation; Phaseolus vulgaris; nucleotide sequences; provenance; detection; mycelium; chemotaxonomy; leaves; Show all 11 Subjects
Abstract:
... Specific detection of the two major groups of Phaeoisariopsis griseola (Andean and Mesoamerican) from infected common bean (Phaseolus vulgaris) leaves was achieved by amplification of different-sized DNA fragments with polymerase chain reaction (PCR) using group-specific primer pairs. These primer pairs were designed based on DNA sequences of cloned random amplified polymorphic DNA (RAPD) fragment ...