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- Pinto, M. Carmen, et al. Show all 2 Author
- Zeitschrift für Naturforschung B 2014 v.42 no.10 pp. 1343-1348
- absorption; agarose; chromatography; enzyme inhibitors; fluorescence emission spectroscopy; isozymes; linoleic acid; lipoxygenase; liver; molecular weight; neutrophils; nordihydroguaiaretic acid; pH; rats
- ... Lipoxygenase was purified from rat liver cytosolic fraction by a method involving two successive chromatographic steps on Sephacryl S-200 and Phenyl Sepharose CL-4B. The enzyme has a molecular weight of 96 Kdal and it seems to be composed of two identical subunits. Chromatofocusing of the enzyme revealed a single band of activity at pi 6.3. The enzyme activity of the purified fraction showed maxim ...