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- Prefot, Petra, et al. Show all 9 Authors
- Journal of Proteome Research 2015 v.14 no.6 pp. 2500-2510
- histidine; pH; phosphopeptides; proteome; resins; serine; threonine; tyrosine
- ... We present a novel tandem mass tag solid-phase amino labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatized (C18) solid supports. This method can reduce the number of steps required in complex protocols, saving time and potentially reducing sample loss. In our global phosphopeptide profiling workflow (SysQuant), we can cut 24 h from the protocol while i ...