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- Pu, Xiumin, et al. Show all 10 Authors
- BMC biotechnology 2019 v.19 no.1 pp. 9
- Agrobacterium; RNA; Southern blotting; bioinformatics; cotyledons; crops; enzymes; fatty acid composition; gene editing; genes; linoleic acid; loci; mutants; near-infrared spectroscopy; nucleotide sequences; oleic acid; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; seeds; sequence analysis; site-directed mutagenesis; soybeans; transgenic plants
- ... BACKGROUND: Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system has appeared a promising tool in site-directed mutagenesis due to its innovative utilization in different branches of biology. CRISPR-Cas9 nuclease system have supersedes all pre ...