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- Ringel, Peter D., et al. Show all 6 Authors
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.87 pp. 99-104
- linoleic acid; Pleurotus sapidus; hydrocarbons; linoleate 13S-lipoxygenase; odors; high performance liquid chromatography; amino acid sequences; pH; temperature; enzyme activity; catalytic activity; hydroperoxides; grapefruits; fungi
- ... A dioxygenase from the edible basidiomycete Pleurotus sapidus, originally researched because of its distinct ability to convert the sequiterpene (+)-valencene to the valuable grapefruit aroma (+)-nootkatone, was identified as a potent lipoxygenase (LOXPsa1). Kinetic parameters, pH and temperature optima of the pure recombinant enzyme were determined using linoleic acid as the substrate. Km, vmax, ...