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- Sedgwick, S.G., et al. Show all 9 Authors
- Proceedings of the National Academy of Sciences of the United States of America 1996 v.93 no.7 pp. 2801-2806
- genetic transformation; transcription factors; recombinant DNA; Xenopus laevis; plasmid vectors; Schizosaccharomyces pombe; transgenic animals; Saccharomyces cerevisiae; beta-galactosidase; mice; gene expression; transcription (genetics); DNA-binding proteins; reporter genes; mutation; promoter regions; genetic markers
- ... Transposon Tn1000 has been adapted to deliver novel DNA sequences for manipulating recombinant DNA. The transposition procedure for these "tagged" Tn1000s is simple and applicable to most plasmids in current use. For yeast molecular biology, tagged Tn1000s introduce a variety of yeast selective markers and replication origins into plasmids and cosmids. In addition, the beta-globin minimal promoter ...