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Severeacuterespiratorysyndromecoronavirus2, etc ; air; bioaerosols; environment; equipment; filtration; ionization; risk; virion; Show all 9 Subjects
Abstract:
... The transmission rate of SARS-CoV-2 is higher in collective transport than in other public environments. Transport companies require preventive strategies to mitigate airborne risk of contagion which not imply responsible use at the individual level. Air purification systems, such as UV-C or needle-tip bipolar ionization, are attractive alternatives. However, only a few studies addressing the vali ...
Severeacuterespiratorysyndromecoronavirus2, etc ; RNA; colorimetry; detection limit; genes; humans; mouth; nose; recombinase polymerase amplification; Show all 9 Subjects
Abstract:
... In this paper we present a new method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), targeting a specific region “N gene.” Under isothermal reaction conditions, we integrated ligation (Lig; high selectivity) and recombinase polymerase amplification (RPA; high sensitivity) processes, obtaining a robust method of detection. For point-of-care testing, we incorporat ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; heparan sulfate; heparin; pandemic; peptidyl-dipeptidase A; therapeutics; China; Show all 8 Subjects
Abstract:
... In December 2019, the global coronavirus disease 2019 (COVID-19) pandemic began in Wuhan, China. COVID-19 is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infects host cells primarily through the angiotensin-converting enzyme 2 (ACE2) receptor. In addition to ACE2, several studies have shown the importance of heparan sulfate (HS) on the host cell surface as a co ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; air quality; humans; pandemic; public policy; socioeconomics; spatial variation; uncertainty; wildlife; China; Show all 11 Subjects
Abstract:
... The coronavirus disease 2019 (COVID-19) pandemic is an unprecedented worldwide health crisis. Many previous research studies have found and investigated its links with one or some natural or human environmental factors. However, a review on the relationship between COVID-19 incidence and both the natural and human environment is still lacking. This review summarizes the inter-correlation between C ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; capital; ecological footprint; environment; gross domestic product; people; social capital; storms; vaccination; Show all 10 Subjects
Abstract:
... The Covid-19 crisis has caused several social-related issues; the sanitary is, perhaps, the most significant one. Lockdowns and vaccination were implemented to fight the Covid-19 virus. From a sustainability perspective, Covid-19 has been considered a meaningful crisis driver that has affected nations' economies and social and natural capitals. The literature presents clues that effects appear to ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; RNA; air temperature; data collection; epidemiology; meta-analysis; monitoring; systematic review; wastewater; watersheds; Show all 11 Subjects
Abstract:
... Wastewater-based epidemiology (WBE) has been considered as a promising approach for population-wide surveillance of coronavirus disease 2019 (COVID-19). Many studies have successfully quantified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA concentration in wastewater (CRNA). However, the correlation between the CRNA and the COVID-19 clinically confirmed cases in the correspondi ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; RNA; disease surveillance; disease transmission; environment; hospitals; monitoring; pandemic; public health; resource allocation; wastewater; Show all 12 Subjects
Abstract:
... Public health surveillance systems for COVID-19 are multifaceted and include multiple indicators reflective of different aspects of the burden and spread of the disease in a community. With the emergence of wastewater disease surveillance as a powerful tool to track infection dynamics of SARS-CoV-2, there is a need to integrate and validate wastewater information with existing disease surveillance ...
Severeacuterespiratorysyndromecoronavirus2, etc ; COVID-19 infection; antibodies; biosensors; drug development; human health; mutation; pandemic; rapid methods; reverse transcriptase polymerase chain reaction; viruses; Show all 11 Subjects
Abstract:
... Currently, the coronavirus disease 2019 (COVID-19) pandemic is ravaging the world, causing serious crisis in economy and human health. The top priority is the detection and drug development of the novel coronavirus. The gold standard for real-time diagnosis of coronavirus disease is the reverse transcription-polymerase chain reaction (RT-PCR), which is usually operatively complex and time-consumin ...
... The current approaches of diagnostic platforms for detecting SARS-CoV-2 infections mostly relied on adapting the existing technology. In this work, a simple and low-cost electrochemical sensing platform for detecting SAR-CoV-2 antigen was established. The proposed sensor combined the innovative disposable paper-based immunosensor and cost-effective plant-based anti-SARS-CoV-2 monoclonal antibody C ...
... During the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection methods from wastewater have been developed, there are obstacles to their social implem ...
... With rapid growing of environmental contact infection, more and more attentions are focused on the precise and absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus on cold chain foods via point-of-care test (POCT). In this work, we propose a hydrogel-mediated reverse transcription loop-mediated isothermal amplification (RT-LAMP) for ultrafast and absolute q ...
... The detection of infectious SARS-CoV-2 in food and food packaging associated with the cold chain has raised concerns about the possible transmission pathway of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in foods transported through cold-chain logistics and the need for novel decontamination strategies. In this study, the effect of electron beam (E-beam) irradiation on the inactiv ...
Severeacuterespiratorysyndromecoronavirus2, etc ; genes; research; Show all 3 Subjects
Abstract:
... High-resolution melting (HRM) analysis was performed to detect G339D and D796Y variations in the SARS-CoV-2 Omicron variant spike protein. We employed two-step PCR consisting of the first RT–PCR and the second nested PCR to prepare the amplicon for HRM analysis. The melting temperatures (Tm) of the amplicon from the cDNA of the Omicron variant receptor binding domain (RBD) were 73.1 °C (G339D vari ...
Flavia R.O. Barros; Deborah C.A. Leite; Larissa J. Guimarães; Juliana M.H. Lopes; Marina W. Vasconcelos; Luciane X. Ferreira; Sandrieli Gonçalves; Victor G.F. Pastre; Gabriela Pereira; Alex B. Trentin; Naiana C. Gabiatti; Betty C. Kuhn; Juliana M.K.C. Perseguini; Simone N. Wendt; Nédia C. Ghisi
Severeacuterespiratorysyndromecoronavirus2, etc ; RNA; pandemic; Show all 3 Subjects
Abstract:
... The rapid spread of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led the world to a pandemic. Therefore, rapid, sensitive, and reproducible diagnostic tests are essential to indicate which measures should be taken during pandemics. We retrospectively tested unextracted nasopharyngeal samples from consecutive patients with suspected SARS-CoV-2 infection (n = 334), and compar ...
Severeacuterespiratorysyndromecoronavirus2, etc ; evolution; microbiology; Show all 3 Subjects
Abstract:
... The sudden emergence of the SARS-CoV-2 Omicron variant is causing major global concern due to its high number of mutations compared to previous variants, which is a relatively rare but significant event that can change the course of viral evolution, the occurrence of which might have huge consequences for the natural evolution of species in general, prompting us to rethink our knowledge on evoluti ...
Severeacuterespiratorysyndromecoronavirus2, etc ; mutation; virology; Show all 3 Subjects
Abstract:
... Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the detection of P681H to distinguish between these two variants. Our experience highlights the importa ...
Severeacuterespiratorysyndromecoronavirus2, etc ; flavonoids; mammals; Show all 3 Subjects
Abstract:
... The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been shown to hijack angiotensin converting enzyme 2 (ACE2) for entry into mammalian cells. A short isoform of ACE2, termed deltaACE2 (dACE2), has recently been identified. In contrast to ACE2, the short dACE2 isoform lacks the ability to bind the spike protein of SARS-CoV-2. Several studies have proposed that ...
Severeacuterespiratorysyndromecoronavirus2, etc ; cattle; whey; Show all 3 Subjects
Abstract:
... Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1–1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa, and omicron, which contain 10, eight, seven, and over 30 mutations in the spike protein, respective ...
Venice Servellita; Abdullah M. Syed; Mary Kate Morris; Noah Brazer; Prachi Saldhi; Miguel Garcia-Knight; Bharath Sreekumar; Mir M. Khalid; Alison Ciling; Pei-Yi Chen; G. Renuka Kumar; Amelia S. Gliwa; Jenny Nguyen; Alicia Sotomayor-Gonzalez; Yueyuan Zhang; Edwin Frias; John Prostko; John Hackett; Raul Andino; Debra A. Wadford; Carl Hanson; Jennifer Doudna; Melanie Ott; Charles Y. Chiu
Severeacuterespiratorysyndromecoronavirus2, etc ; antibodies; vaccines; viruses; Show all 4 Subjects
Abstract:
... Virus-like particle (VLP) and live virus assays were used to investigate neutralizing immunity against Delta and Omicron SARS-CoV-2 variants in 259 samples from 128 vaccinated individuals. Following Delta breakthrough infection, titers against WT rose 57-fold and 3.1-fold compared with uninfected boosted and unboosted individuals, respectively, versus only a 5.8-fold increase and 3.1-fold decrease ...
Severeacuterespiratorysyndromecoronavirus2, etc ; immunogenicity; vaccines; viruses; Show all 4 Subjects
Abstract:
... During the assessment and licensing of novel vaccines, as well as post licensure follow up, it is critical to have reliable immunogenicity testing methods that relate well to real life protection [...] ...