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1. Rapid Lipid Induction in Chlorella sp. by UV-C Radiation

Author:
Sharma, Kalpesh K.,   et al.   ; Li, Yan; Schenk, Peer M.;     Show all 3 Authors
Source:
BioEnergy research 2015 v.8 no.4 pp. 1824-1830
ISSN:
1939-1234
Subject:
Chlorella; biodiesel; energy; fatty acid composition; fatty acid metabolism; feedstocks; flow cytometry; fluorescence; fuel production; gas chromatography-mass spectrometry; microalgae; saturated fatty acids; ultraviolet radiation; unsaturated fatty acids
Abstract:
... Rapid induction of lipid accumulation in microalgae is an important prerequisite towards the use of microalgae as a feedstock for biodiesel production. In this study, we present a novel approach to induce lipids in Chlorella sp. within 24 h by short-term UV-C radiation (UVR) stress at different energy intensities ranging from 0 to 1000 mJ/cm². Increase in the lipid fluorescence was measured by Nil ...
DOI:
10.1007/s12155-015-9633-y

2. Lipid extraction from wet Chaetoceros muelleri culture and evaluation of remaining defatted biomass

Author:
Sharma, Kalpesh,   et al.   ; Naghdi, Forough Ghasemi; Bai, Xue; Thomas-Hall, Skye R.; Schenk, Peer M.;     Show all 5 Authors
Source:
Algal research 2016 v.20 pp. 205-212
ISSN:
2211-9264
Subject:
Chaetoceros muelleri; biodiesel; biomass; carbohydrates; cost effectiveness; drying; ethanol; ethanol production; fatty acid methyl esters; feedstocks; fermentation; heat treatment; hexane; microalgae; oils; osmotic stress; slurries; solvents; triacylglycerols; ultraviolet radiation
Abstract:
... Microalgae-based lipids are considered a good feedstock for various applications, including biodiesel and omega-3-rich oil production. However, developing new lipid extraction and recovery techniques are required to make microalgal oil a more economically competitive feedstock. To avoid the costly process of complete drying of algal biomass, extraction from wet slurry was improved. Potentially cos ...
DOI:
10.1016/j.algal.2016.10.011

3. Isolation of High-Lipid Tetraselmis suecica Strains Following Repeated UV-C Mutagenesis, FACS, and High-Throughput Growth Selection

Author:
Sharma, Kalpesh,   et al.   ; Lim, David K. Y.; Schuhmann, Holger; Schenk, Peer M.;     Show all 4 Authors
Source:
BioEnergy research 2015 v.8 no.2 pp. 750-759
ISSN:
1939-1234
Subject:
Tetraselmis suecica; biodiesel; breeding; cell growth; feedstocks; flow cytometry; genetic engineering; microalgae; mutagenesis; screening; triacylglycerols; ultraviolet radiation
Abstract:
... Mutagenesis and selection of microalgae can be used for accelerated breeding of elite strains, providing a significant advantage over genetic engineering as prior biochemical and genetic information is not required. Ultraviolet (UV)-C-induced mutagenesis combined with fluorescence-activated cell sorting (FACS) and microtiter plate reader cell density screening was used to produce Tetraselmis sueci ...
DOI:
10.1007/s12155-014-9553-2