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- Shen, Ai Xi, et al. Show all 5 Authors
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.95 pp. 55-61
- Escherichia coli; amino acids; ammonium compounds; catalytic activity; cotton; epicatechin; epigallocatechin; feruloyl esterase; genes; genomic libraries; magnesium; manganese; molecular weight; nickel; pH; screening; sequence analysis; soil; tannase; tannins; temperature
- ... A gene (tan410) encoding a feruloyl esterase was isolated by screening a cotton soil metagenomic library. Sequence analysis revealed that tan410 encodes a protein of 520 amino acids with a predicted molecular weight of 55kDa. The gene was further expressed in Escherichia coli BL21 (DE3) using a pET expression system. The recombinant enzyme was purified and characterized. Its optimum temperature an ...