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Shigatoxin, etc ; DNA; Escherichia coli O157; antimicrobial properties; bacteria; biofilm; carvacrol; cineole; exopolysaccharides; flagellum; food safety; fresh produce; genes; intimin; mechanism of action; quorum sensing; sanitizers; transcription (genetics); virulence; Show all 19 Subjects
Abstract:
... The objective of this study was to evaluate the mechanism of the synergistic antimicrobial effect of cineole (CN) and carvacrol (CR) on E. coli and its application on fresh-cut cucumbers. Herein, the CN/CR synergy could effectively inhibit E. coli by destroying its bacterial cell structure and causing the leakage of intracellular organic matters. For the first time, CN/CR synergy interaction on E. ...
Shigatoxin; Shigatoxin-producing Escherichia coli, etc ; adhesins; antibiotics; average daily gain; edema; farms; genes; herds; morbidity; mortality; vaccination; vaccines; weight gain; Show all 14 Subjects
Abstract:
... Oedema disease of weaned piglets is caused by shigatoxigenic Escherichia coli (STEC), typically harbouring the stx2e gene and F18 adhesins. The aim of this study was to assess the effect of a commercially available oedema disease vaccine on the zootechnical performance, mortality and individual antibiotic treatment in a herd, in which non-typical STEC strains without F18 adhesin have been identifi ...
Shigatoxin-producing Escherichia coli, etc ; detection limit; monitoring; serotypes; Alberta; Show all 5 Subjects
Abstract:
... Non-O157 Shiga toxin-producing E. coli (STEC) can cause outbreaks that have great economic and health impact. Since the implementation of STEC screening in Alberta in 2018, it is also essential to have a molecular serotyping method with faster turnaround time for cluster identification and surveillance purposes. This study sought to perform molecular serotyping of the top six non-O157 (O26, O45, O ...
... Epidemiological evidence of Shiga toxin-producing Escherichia coli (STEC) infections associated with the consumption of contaminated pork highlight the need for increased awareness of STEC as an emerging pathogen in the pork supply chain. The objective of this review is to contribute to our understanding of raw pork products as potential carriers of STEC into the food supply. We summarize and crit ...
Shigatoxin-producing Escherichia coli, etc ; Holstein; feces; feedlots; mouth; pollution; summer; Show all 7 Subjects
Abstract:
... The purpose of this study was to use nonpathogenic Escherichia coli as a surrogate to assess the transmission dynamics of Shiga toxin–producing E. coli in cattle. Two studies were performed. For the first study, 3 nonpathogenic E. coli strains were orally inoculated into 12 weaned Holsteins calves. Calves were sampled daily the first week and 3 times a week for the following 4 wk. The nonpathogeni ...
Shigatoxin-producing Escherichia coli, etc ; Campylobacter; Listeria monocytogenes; Salmonella; Yersinia; summer; Ontario; Show all 7 Subjects
Abstract:
... In Canada, enteric infections cause significant health and economic burden. We evaluated the individual characteristics of laboratory-confirmed cases of Campylobacter spp. (n = 28,728), non-typhoidal Salmonella spp. (n = 22,640), Yersinia spp. (n = 1674), Verotoxin-producing Escherichia coli (VTEC; n = 1340), and Listeria monocytogenes (n = 471), reported between 2010 and 2017 inclusive, in Ontari ...
Shigatoxin-producing Escherichia coli, etc ; Salmonella; Staphylococcus aureus; ground beef; hygiene; risk; Argentina; Show all 7 Subjects
Abstract:
... The aim of this work was to evaluate the hygienic-sanitary conditions of butcher shops in Tandil, Buenos Aires Province, by estimating the risk based on good manufacturing and hygiene practices, through surveys of the establishments. The analysis was performed using a scale of 1-100, and classifying them as high risk (0-40), moderate risk (41-70) or low risk (71-100). The presence of Salmonella sp ...
Shigatoxin-producing Escherichia coli, etc ; HACCP; beef; boning; sanitation; slaughter; slaughterhouses; zero tolerance; Show all 8 Subjects
Abstract:
... The aim of this work was to reinforce actions tending to reduce Shiga toxin-producing Escherichia coli (STEC) in beef products from an Argentinean commercial abattoir implementing Hazard Analysis and Critical Control Point (HACCP) practices. An environmental stx map was built with 421 environmental samples from the slaughter, quartering, cool chamber and deboning sectors (February-May 2013). For s ...
Shigatoxin; Shigatoxin-producing Escherichia coli, etc ; cattle; enteropathogenic Escherichia coli; genes; genetic relationships; genomics; humans; microbiology; monitoring; multilocus sequence typing; phylogeny; public health; serotypes; sheep; single nucleotide polymorphism; virulence; China; Netherlands; Sweden; Show all 20 Subjects
Abstract:
... AIMS: There are knowledge gaps regarding STEC and EPEC strains in livestock in Jiangsu, China. This study aimed to evaluate the potential public health significance of STEC and EPEC strains isolated from livestock by determining the serotypes, virulence profiles, and genetic relationship with international STEC strains. METHODS AND RESULTS: A total of 68 STEC and 37 EPEC strains were obtained from ...
Shigatoxin; Shigatoxin-producing Escherichia coli, etc ; beef; diarrhea; genetic relationships; genetic similarity; genomics; geographical distribution; hemolytic uremic syndrome; host range; host specificity; human diseases; mutton; serotypes; single nucleotide polymorphism; toxicity; virulence; China; Show all 18 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) can cause diseases ranging from mild diarrhea to fatal extra-intestinal hemolytic uremic syndrome (HUS). Shiga toxin (Stx) is the key virulence factor in STEC, two Stx types (Stx1 and Stx2) and several subtypes varying in sequences, toxicity, and host specificity have been identified. Stx2l is a newly-designated subtype related to human disease but lac ...
... Shiga toxin-producing E. coli (STEC), widespread pathogens associated with severe foodborne disease, can contaminate milk during the milking process through faecal matter and survive or grow during cheese making if a pasteurization treatment has not been applied. Thus, a stochastic “farm-to-fork” model was developed to assess the risk of human infection by O157 STEC, one of the main pathogenic ser ...
Shigatoxin-producing Escherichia coli, etc ; Listeria monocytogenes; Salmonella; chlorine; chlorine dioxide; decontamination; food safety; Show all 7 Subjects
Abstract:
... Small-scale studies have shown that chlorine dioxide gas, ClO2(g), was effective for decontamination of produce, nuts, and spices. This study conducted a pilot-scale evaluation to identify effective ClO2(g) treatment parameters for commercial-scale applications. The gas was produced by a generator utilizing chlorite and chlorine gas for decontamination of Shiga toxin-producing Escherichia coli (ST ...
Shigatoxin-producing Escherichia coli, etc ; agar; biofilm; cellulose; coculture; food microbiology; genes; gentian violet; meat; Show all 9 Subjects
Abstract:
... This study investigated the impact of meat processing surface bacteria (MPB) on biofilm formation by non-O157 Shiga toxin-producing Escherichia coli (STEC), and potential links between biofilm formation by STEC and biofilm-related genes in their genomes. Biofilm development by 50 MPB and 6 STEC strains in mono- and co-cultures was assessed by the crystal violet staining method, and their expressio ...
Shigatoxin-producing Escherichia coli, etc ; Salmonella; food safety; lactic acid; peracetic acid; wheat; wheat flour; Show all 7 Subjects
Abstract:
... Wheat flour contaminated with Salmonella or Shiga toxin–producing Escherichia coli (STEC) has been linked to foodborne outbreaks and recalls. The survival of five strains of rifampicin-resistant Salmonella and STEC on wheat berries was evaluated during tempering (hydration). Wheat berries were inoculated with Salmonella (cocktail) or STEC (individual strains or cocktail) and dried at ambient condi ...
Shigatoxin, etc ; Gibbs free energy; ceramides; chemical structure; cholesterol; fatty acids; liquids; sphingomyelins; surface tension; Show all 9 Subjects
Abstract:
... The glycosphingolipid Gb₃ is a specific receptor of the bacterial Shiga toxin (STx). Binding of STx to Gb₃ is a prerequisite for its internalization into the host cells, and the ceramide’s fatty acid of Gb₃ has been shown to influence STx binding. In in vitro studies on liquid ordered (lₒ)/liquid disordered (ld) coexisting artificial membranes, Shiga toxin B (STxB) binds solely to lₒ domains, thus ...
Shigatoxin-producing Escherichia coli, etc ; genes; hospitals; public health; sewage treatment; wastewater; wastewater treatment; water; Nepal; Show all 9 Subjects
Abstract:
... Inadequately treated effluents discharged from wastewater treatment plants (WWTPs) severely affect the environment and the surrounding population. This study analyzed the presence of the Shiga toxin-producing Escherichia coli (STEC) genes, stx1, and stx2, and the E. coli gene, sfmD, in municipal WWTP A (n = 11) and B (n = 11) where the reductions were also evaluated; hospitals (n = 17), sewage tre ...
... Wild birds, particularly silver gulls (Chroicocephalus novaehollandiae) that nest near anthropogenic sites, often harbour bacteria resistant to multiple antibiotics, including those considered of clinical importance. Here, we describe the whole genome sequence of Escherichia coli isolate CE1867 from a silver gull chick sampled in 2012 that hosted an I1 pST25 plasmid with blaSHV₋₁₂, a β-lactamase g ...
Shigatoxin, etc ; Campylobacter; Salmonella; biotypes; food safety; game meat; genes; livestock; supply chain; traceability; wild boars; zoonoses; Italy; Show all 13 Subjects
Abstract:
... Wild boars can be infected with several foodborne pathogens which may be transmitted to humans through the consumption of their meat, but currently, data of their prevalence are still limited. The present study aimed to evaluate the presence of enteric pathogens in wild boar meat samples killed in the Campania region. Twentyeight wild boar meat samples were analyzed for the detection of Salmonella ...
... Shiga toxin producing Escherichia coli (STEC) are common etiological agents of food borne illnesses and outbreaks, most often caused by consuming contaminated beef products, followed by raw vegetables and dairy products. Patients infected with E. coli O157 are more likely hospitalized than patients infected with non-O157 STEC, making E. coli O157 an important target for microbiological interventio ...
Shigatoxin-producing Escherichia coli, etc ; beef; food safety; monitoring; pork; quantitative polymerase chain reaction; red meat; serotypes; virulence; Show all 9 Subjects
Abstract:
... Virulent strains of Shiga toxin-producing Escherichia coli (STEC) serogroups O103 and O121 are considered adulterants in beef. Two high-resolution melting (HRM) real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. The O103 HRM assay offered the possibility to distinguish clearly STEC O103:H2 fro ...