PubAg

Main content area

You searched for: Subject "Shiga toxin" Remove constraint Subject: "Shiga toxin"
Start Over

Search

1,005  Search Results

« Previous | 1 - 20 of 1,005 |
Number of results to display per page

Search Results

1. Top-down and middle-down proteomic analysis of Shiga toxin using MALDI-TOF-TOF mass spectrometry

Author:
Fagerquist, Clifton K.; Zaragoza, William J.
Source:
MethodsX 2019 v.6 pp. 815-826
ISSN:
2215-0161
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; agar; antibiotics; bacteria; bacteriophages; ciprofloxacin; culture media; desorption; electric potential difference; ionization; peptides; proteomics; salts; tandem mass spectrometry;     Show all 15 Subjects
Abstract:
... The method describes a step-by-step process for analysis of putative Shiga toxin-producing Escherichia coli (STEC) for expression of Shiga toxin (Stx). The technique utilizes antibiotic induction, mass spectrometry and top-down/middle-down proteomic analysis. Stx expression is induced by overnight culturing of a STEC strain on Luria-Bertani agar (LBA) supplemented with DNA-damaging antibiotics. Cu ...
DOI:
10.1016/j.mex.2019.04.011

2. Using Nanospray Liquid Chromatography and Mass Spectrometry to Quantitate Shiga Toxin Production in Environmental Escherichia coli Recovered from a Major Produce Production Region in California

Author:
Silva, Christopher J.; Lee, Bertram G.; Yambao, Jaszemyn C.; Erickson-Beltran, Melissa L.; Quiñones, Beatriz
Source:
Journal of agricultural and food chemistry 2018 v.67 no.5 pp. 1554-1562
ISSN:
1520-5118
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; bioassays; liquid chromatography; mass spectrometry; operon; serotypes; California;     Show all 8 Subjects
Abstract:
... A set of 45 environmental strains of Shiga toxin producing Escherichia coli (STEC) from three California counties were analyzed for Shiga toxin production by nanospray liquid chromatography–mass spectrometry and Vero cell bioassay. The STEC in this set comprised six serotypes ((O113:H21, O121:H19, O157:H7, O6:H34, O177:H25, and O185:H7) each containing either the stx₂ₐ or stx₂c operon. Six of the ...
DOI:
10.1021/acs.jafc.8b05324

3. Evaluation of PCR-based methods for the identification of enteroaggregative hemorrhagic Escherichia coli in sprouts

Author:
Rotundo, Luca; Amagliani, Giulia; Carloni, Elisa; Omiccioli, Enrica; Magnani, Mauro; Paoli, George
Source:
International journal of food microbiology 2019 v.291 pp. 59-64
ISSN:
0168-1605
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; agar; agglutination; alfalfa; genes; latex; mung beans; quantitative polymerase chain reaction;     Show all 8 Subjects
Abstract:
... In this study real-time PCR assays were evaluated for the detection of enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104:H4 in artificially contaminated mung bean and/alfalfa sprouts inoculated with 1, 10, and 100 CFU of EAHEC O104:H4 per 25 g sample (20, 10, and 2 replicates respectively). After selective culture enrichment the samples were tested using commercial real-time PCR kits det ...
DOI:
10.1016/j.ijfoodmicro.2018.11.011

4. ArgO145, a Stx2a prophage of a bovine O145:H- STEC strain, is closely related to phages of virulent human strains

Author:
Krüger, A.; Burgán, J.; Friedrich, A.W.; Rossen, J.W.A.; Lucchesi, P.M.A.
Source:
Infection, genetics, and evolution 2018 v.60 pp. 126-132
ISSN:
1567-1348
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; bacteriophages; cattle; human diseases; humans; patients; regulatory proteins; structural genes; virulence;     Show all 10 Subjects
Abstract:
... Shiga toxins (Stx) are the main virulence factor of a pathogroup of Escherichia coli strains that cause severe human diseases. These toxins are encoded in prophages (Stx prophages), and generally their expression depends on prophage induction. Several studies have reported high diversity among both Stx prophages and Stx. In particular, the toxin subtype Stx2a is associated with high virulence and ...
DOI:
10.1016/j.meegid.2018.02.024

5. General detection of Shiga toxin 2 and subtyping of Shiga toxin 1 and 2 in Escherichia coli using qPCR

Author:
Zhi, Shuai; Szelewicki, Jonas; Ziebell, Kim; Parsons, Brendon; Chui, Linda
Source:
Journal of microbiological methods 2019 v.159 pp. 51-55
ISSN:
0167-7012
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; Shiga-like toxin 1; Shiga-like toxin 2; bioactive properties; cross reaction; epidemiology; gastrointestinal system; genes; monitoring; pathogens; public health; quantitative polymerase chain reaction; risk assessment;     Show all 14 Subjects
Abstract:
... Shiga toxin-producing E. coli (STEC) is a gastrointestinal pathogen and has been recognized as one of the serious problems in public health. Shiga toxin genes (stx) can be grouped into different types according to their differences in sequence and biological activities. The two main groups of stx are stx1 and stx2 with each group containing several subtypes. It is essential to develop rapid stx1/s ...
DOI:
10.1016/j.mimet.2019.02.008

6. Bacteriological analysis of wheat flour associated with an outbreak of Shiga toxin-producing Escherichia coli O121

Author:
Gill, Alexander; Carrillo, Catherine; Hadley, Micaela; Kenwell, Robyn; Chui, Linda
Source:
Food microbiology 2019 v.82 pp. 474-481
ISSN:
0740-0020
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; agar; coliform bacteria; most probable number technique; outbreak investigation; wheat flour; Canada;     Show all 7 Subjects
Abstract:
... A 2016/2017 outbreak of Shiga toxin-producing Escherichia coli (STEC) O121 in Canada, was linked to wheat flour, milled at a single facility on three consecutive days in October 2016. Most Probable Number (MPN) estimates of the concentration of STEC O121 in the recalled flour were made using the results of qualitative testing conducted during the outbreak investigation and from analysis of 5 × 2.5 ...
DOI:
10.1016/j.fm.2019.03.023

7. Bootstrap parametric GB2 and bootstrap nonparametric distributions for studying shiga toxin-producing Escherichia coli strains growth rate variability

Author:
Quinto, E.J.; Marín, J.M.; Caro, I.; Mateo, J.; Redondo-del-Río, M.P.; de-Mateo-Silleras, B.; Schaffner, D.W.
Source:
Food research international 2019 v.120 pp. 829-838
ISSN:
0963-9969
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; confidence interval; dairy products; food pathogens; meat; models; serotypes; temperature; vegetables; water pollution;     Show all 10 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (VTEC) strains, including the serotype O157:H7, are considered foodborne pathogens. Transmission occurs through consumption of undercooked meat, unpasteurized dairy products, vegetables, or contaminated water. The variability of pathogenic and nonpathogenic E. coli strains growth parameters at different temperatures and in different media was studied. Bootstr ...
DOI:
10.1016/j.foodres.2018.11.045

8. Fate of generic and Shiga toxin-producing Escherichia coli (STEC) in Central Florida surface waters and evaluation of EPA Worst Case water as standard medium

Author:
Topalcengiz, Zeynal; Danyluk, Michelle D.
Source:
Food research international 2019 v.120 pp. 322-329
ISSN:
0963-9969
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; Escherichia coli K12; cycloheximide; harvesting; microorganisms; monitoring; population dynamics; surface water; temperature; Florida;     Show all 10 Subjects
Abstract:
... Agricultural water is considered as one of the main contamination source for produce prior to harvest. The purpose of study was to evaluate the fate of Shiga toxin-producing Escherichia coli (STEC), and generic E. coli in Central Florida agricultural surface water at different temperatures and the potential use of EPA Worst Case water as a standardized media. Cocktails of STEC (O145, O104, O111, O ...
DOI:
10.1016/j.foodres.2019.02.045

9. Mass spectrometry-based Shiga toxin identification: A clinical validation

Author:
Zhang, Lijie; Zhang, Lu; Du, Yanhua; Li, Xingle; Unger, Mark; Davlut, Derek; Sloan, Angela; Armstrong, Glen D.; Wang, Gehua; Cheng, Keding
Source:
Journal of proteomics 2019 v.198 pp. 145-150
ISSN:
1874-3919
Subject:
Shiga toxin,   etc   ; Escherichia coli; antibiotics; ciprofloxacin; databases; death; drug therapy; foodborne illness; liquid chromatography; mitomycin; proteomics; tandem mass spectrometry;     Show all 12 Subjects
Abstract:
... After we published our preliminary study on the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and curated E. coli toxin databases on the identification of E. coli Shiga toxins (Stxs) in the Journal of Proteomics in year 2018, we were encouraged to further refine the method and test clinical isolates. In this study, different concentrations of mitomycin C (MMC) and ciprofloxacin ...
DOI:
10.1016/j.jprot.2019.01.020

10. Interlaboratory Evaluation of the U.S. Food and Drug Administration Escherichia coli Identification Microarray for Profiling Shiga Toxin–Producing Escherichia coli

Author:
Patel, Isha R.; Gangiredla, Jayanthi; Lacher, David W.; Mammel, Mark K.; Bagi, Lori; Baranzoni, Gian Marco; Fratamico, Pina M.; Roberts, Elizabeth L.; DebRoy, Chitritia; Lindsey, Rebecca L.; Stoneburg, Devon V.; Martin, Haley; Smith, Peyton; Strockbine, Nancy A.; Elkins, Christopher A.; Scheutz, Flemming; Feng, Peter C. H.
Source:
Journal of food protection 2018 v.81 no.8 pp. 1275-1282
ISSN:
0362-028X
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; Food and Drug Administration; O-antigens; alleles; antigens; intimin; loci; microarray technology; quality control;     Show all 10 Subjects
Abstract:
... The U.S. Food and Drug Administration Escherichia coli Identification (FDA-ECID) microarray provides rapid molecular characterization of E. coli. The effectiveness of the FDA-ECID for characterizing Shiga toxin–producing E. coli (STEC) was evaluated by three federal laboratories and one reference laboratory with a panel of 54 reference E. coli strains from the External Quality Assurance program. S ...
DOI:
10.4315/0362-028X.JFP-18-052
PubMed:
29985068
PubMed Central:
PMC6193752

11. Overview of the role of Shiga toxins in porcine edema disease pathogenesis

Author:
Casanova, Natalia A.; Redondo, Leandro M.; Dailoff, Gabriela C.; Arenas, David; Fernández Miyakawa, Mariano E.
Source:
Toxicon 2018 v.148 pp. 149-154
ISSN:
0041-0101
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; amino acids; brain; edema; endothelial cells; enterocytes; feces; glycosphingolipids; hemolytic uremic syndrome; humans; necrosis; pathogenesis; piglets; virulence;     Show all 15 Subjects
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) have been implicated as the cause of enterotoxemias, such as hemolytic uremic syndrome in humans and edema disease (ED) of pigs. Stx1 and Stx2 are the most common types found in association with illness, but only Stx2e is associated with disease in the animal host. Porcine edema disease is a serious affection which can lead to dead causing great losses ...
DOI:
10.1016/j.toxicon.2018.04.019

12. Occurrence and antimicrobial resistance of E. coli non-O157 isolated from beef in Mato Grosso, Brazil

Author:
Castro, Vinicius Silva; Teixeira, Larrayane Albuês Carvalho; Rodrigues, Dália dos Prazeres; dos Santos, Luis Fernando; Conte-Junior, Carlos Adam; Figueiredo, Eduardo Eustáquio de Souza
Source:
Tropical animal health and production 2019 v.51 no.5 pp. 1117-1123
ISSN:
0049-4747
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; antibiotic resistance; antibiotics; beef; cytotoxicity; economic impact; financial economics; genes; pathogens; public health; risk; serotypes; slaughterhouses; toxins; vacuum packaging; Brazil;     Show all 16 Subjects
Abstract:
... Shiga toxin–producing Escherichia coli (STEC) is an important public health concern pathogen, as it produces two toxins, Stx1 and Stx2, with cytotoxic capacity. In addition, STEC strains are frequently involved in food outbreaks worldwide, leading to public health challenges and economic losses. In this context, the occurrence and antimicrobial resistance profile of the STEC isolated from fresh be ...
DOI:
10.1007/s11250-018-01792-z

13. Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types

Author:
Bosák, Juraj; Hrala, Matěj; Pirková, Viktória; Micenková, Lenka; Čížek, Alois; Smola, Jiří; Kučerová, Dana; Vacková, Zdenka; Budinská, Eva; Koláčková, Ivana; Šmajs, David
Source:
Veterinary microbiology 2019 v.232 pp. 121-127
ISSN:
0378-1135
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; adhesion; animal diseases; colicins; diarrhea; enterotoxigenic Escherichia coli; humans; intestines; iron; mortality; phylogeny; piglets; plasmids; toxigenic strains; weight gain;     Show all 15 Subjects
Abstract:
... Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses.In this study, frequencies of four phylogenetic g ...
DOI:
10.1016/j.vetmic.2019.04.003

14. Relative response of populations of Escherichia coli and Salmonella enterica to exposure to thermal, alkaline and acidic treatments

Author:
Gill, Alexander; Tamber, Sandeep; Yang, Xianqin
Source:
International journal of food microbiology 2019 v.293 pp. 94-101
ISSN:
0168-1605
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; Salmonella enterica; acid treatment; alkali treatment; beef; cattle; decontamination; heat; heat treatment; humans; lactic acid; models; pH; pork; poultry; serotypes; sodium hydroxide;     Show all 17 Subjects
Abstract:
... We evaluated the relative response of generic Escherichia coli (GEC), Shiga toxin-producing E. coli (STEC) and Salmonella enterica to heat, alkaline or acid treatments. GEC included strains from carcasses (n = 24) and trim (n = 25) at a small beef plant where no decontamination interventions are used and at a large plant where multiple decontamination interventions are used (carcass n = 25 and tri ...
DOI:
10.1016/j.ijfoodmicro.2019.01.007

15. Reduction of Salmonella and Shiga toxin-producing Escherichia coli on alfalfa seeds and sprouts using an ozone generating system

Author:
Mohammad, Zahra; Kalbasi-Ashtari, Ahmad; Riskowski, Gerald; Castillo, Alejandro
Source:
International journal of food microbiology 2019 v.289 pp. 57-63
ISSN:
0168-1605
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; Salmonella; alfalfa; color; exposure duration; food safety; ozonation; ozone; pathogens; risk reduction; seed germination; seeds; serotypes; shelf life;     Show all 14 Subjects
Abstract:
... Several outbreaks of illness have been associated with consumption of alfalfa sprouts contaminated with Shiga toxin-producing Escherichia coli (STEC) and Salmonella. The ozone application was investigated as an intervention. Alfalfa seeds were inoculated with cocktails of 3 Salmonella strains, including serotypes Typhimurium, Agona and Saintpaul, and 3 strains of Shiga toxin-producing Escherichia ...
DOI:
10.1016/j.ijfoodmicro.2018.08.023

16. Detection, Isolation, and Characterization of Shiga Toxin–Producing Escherichia coli in Flour

Author:
Kindle, Patrick; Nuesch-Inderbinen, Magdalena; Cernela, Nicole; Stephan, Roger
Source:
Journal of food protection 2019 v.82 no.1 pp. 164-167
ISSN:
0362-028X
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; European Union; animal pathogens; human diseases; quantitative polymerase chain reaction; risk factors; screening; serotypes; wheat flour; Switzerland; United States;     Show all 11 Subjects
Abstract:
... Wheat flour has recently been described as a novel vehicle for transmission of Shiga toxin–producing Escherichia coli (STEC). Very recently, an outbreak of STEC O121 and STEC O26 infections was linked to flour in the United States. The aim of the present study was to generate baseline data for the occurrence of STEC in flour samples from different retailers in Switzerland. In total, 70 flour sampl ...
DOI:
10.4315/0362-028X.JFP-18-256

17. Genomically Informed Strain-Specific Recovery of Shiga Toxin–Producing Escherichia coli during Foodborne Illness Outbreak Investigations

Author:
Blais, Burton W.; Tapp, Kyle; Dixon, Martine; Carrillo, Catherine D.
Source:
Journal of food protection 2019 v.82 no.1 pp. 39-44
ISSN:
0362-028X
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; antibiotic resistance; antibiotics; foodborne illness; ground beef; high-throughput nucleotide sequencing; markets; microorganisms; models; outbreak investigation; pathogens; serotypes; source attribution;     Show all 13 Subjects
Abstract:
... Next-generation sequencing plays an important role in the characterization of clinical bacterial isolates for source attribution purposes during investigations of foodborne illness outbreaks. Once an illness cluster and a suspect food vehicle have been identified, food testing is initiated for confirmation and to determine the scope of a contamination event so that the implicated lots may be remov ...
DOI:
10.4315/0362-028X.JFP-18-340

18. Tellurite Resistance in Shiga Toxin-Producing Escherichia coli

Author:
Lewis, GentryL.; Jorgensen, QuentinR.; Loy, JohnD.; Moxley, RodneyA.
Source:
Current microbiology 2018 v.75 no.6 pp. 752-759
ISSN:
0343-8651
Subject:
Shiga toxin-producing Escherichia coli,   etc   ; culture media; geometry; intimin; minimum inhibitory concentration; multigene family; potassium; serotypes;     Show all 8 Subjects
Abstract:
... Potassium tellurite (K₂TeO₃) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K₂TeO₃ resistance in STEC is attributable to the ter gene cluster with terD an indicator of the cluster’s presence. Polymerase chain reactions for terD and K₂TeO₃ minimum inhibit ...
DOI:
10.1007/s00284-018-1444-x

19. Antibodies to Shiga toxins in Brazilian cattle

Author:
Yamamoto, Bruno B.; Luz, Daniela; Abreu, Patrícia A.E.; Gotti, Tatiana B.; Vasconcellos, Silvio A.; Piazza, Roxane M.F.; Horton, Denise S.P.Q.
Source:
Toxicon 2017 v.133 pp. 58-62
ISSN:
0041-0101
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; blood serum; cattle; enzyme-linked immunosorbent assay; neutralizing antibodies; virulence;     Show all 7 Subjects
Abstract:
... Cattle are considered a reservoir of Shiga toxin-producing Escherichia coli (STEC). There is no information about the presence of antibodies against Shiga toxins in Brazilian bovine serum. Using ELISA, all sera tested showed antibodies against the two main STEC virulence factors; Stx1 and Stx2. Neutralizing antibodies against Stx1 and/or Stx2 were detected in all but one serum. In conclusion, our ...
DOI:
10.1016/j.toxicon.2017.04.014

20. Improved porcine model for Shiga toxin‐producing Escherichia coli infection by deprivation of colostrum feeding in newborn piglets

Author:
Sato, Toshio; Hamabata, Takashi; Takita, Eiji; Matsui, Takeshi; Sawada, Kazutoshi; Imaoka, Taishi; Nakanishi, Nobuo; Nakayama, Keizo; Tsukahara, Takamitsu
Source:
Animal science journal = 2017 v.88 no.5 pp. 826-831
ISSN:
1344-3941
Subject:
Shiga toxin; Shiga toxin-producing Escherichia coli,   etc   ; breast feeding; colostrum; edema; emaciation; milk; models; mortality; neonates; piglets; toxemia; virulence; weaning;     Show all 14 Subjects
Abstract:
... Porcine edema disease (ED) is a toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)‐producing Escherichia coli (STEC). ED occurs most frequently during the weaning period and is manifested as emaciation associated with high mortality. In our experimental infection with a specific STEC strain, we failed to cause the suppression of weight gain in piglets, which is a typical symptom of ED ...
DOI:
10.1111/asj.12769