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1. Bacterial artificial chromosome-based reverse genetics system for cloning and manipulation of the full-length genome of infectious bronchitis virus

Author:
Shiori Oguro,   et al.   ; Yujin Inayoshi; Erika Tanahashi; Zhifeng Lin; Yasushi Kawaguchi; Toshiaki Kodama; Chihiro Sasakawa;     Show all 7 Authors
Source:
Current research in microbial sciences 2022 v.3 pp. 100155
ISSN:
2666-5174
Subject:
Escherichia coli; Infectious bronchitis virus; antibodies; bacterial artificial chromosomes; bacteriophages; genes; genetic engineering; homologous recombination; immunogenicity; pathogenesis; pathogenicity; phenotype; research; reverse genetics; serotypes; tissue tropism; viral genome; virulent strains
Abstract:
... Avian infectious bronchitis virus (IBV) causes highly contagious respiratory reproductive and renal system diseases in chickens, and emergence of serotypic variants resulting from mutations in the viral S gene hampers vaccine management for IBV infection. In this study, to facilitate the molecular analysis of IBV pathogenesis and the development of a new-generation IBV vaccine, we established a re ...
DOI:
10.1016/j.crmicr.2022.100155