Jump to Main Content
- Shiroyama, Maeka, et al. Show all 9 Authors
- Applied microbiology and biotechnology 2017 v.101 no.18 pp. 6879-6889
- Escherichia coli; carbon; catalytic activity; cysteine; cysteine synthase; gene overexpression; glucose; glycerol; metabolism; microbial growth; phenotype; sulfates; sulfides; sulfites; sulfur; thiosulfate sulfurtransferase; thiosulfates
- ... Sulfate (SO₄ ²⁻) is an often-utilized and well-understood inorganic sulfur source in microorganism culture. Recently, another inorganic sulfur source, thiosulfate (S₂O₃ ²⁻), was proposed to be more advantageous in microbial growth and biotechnological applications. Although its assimilation pathway is known to depend on O-acetyl-L-serine sulfhydrylase B (CysM in Escherichia coli), its metabolism h ...
- Shiroyama, Maeka, et al. Show all 12 Authors
- Journal of bioscience and bioengineering 2015 v.119 no.3 pp. 310-313
- Escherichia coli; biosynthesis; cysteine; derivatization; gene overexpression; regulon; sulfur
- ... We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the l-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated l-homocysteine, suggesting that YciW is involved in l-methionine biosynthesis. ...