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- Silva, Ayrles F.B., et al. Show all 6 Authors
- Food bioscience 2019 v.29 pp. 81-85
- atomic force microscopy; calcium phosphates; cheeses; chymosin; grapes; hydrolysis; kappa-casein; light scattering; micelles; milk; milk clotting; zeta potential
- ... Caseins (αs1-, αs2, β-, and κ-caseins) form the major protein fraction of milk, irrespective of their origins. They are able to form well-ordered colloidal structures in association with colloidal calcium phosphate, named casein micelles. Chymosin-mediated milk coagulation takes place through loss of casein micelles’ stability by hydrolyzing κ-caseins. This process is critical for the quality of c ...
- Silva, Ayrles F.B., et al. Show all 15 Authors
- Food chemistry 2019
- Calotropis procera; Danio rerio; Escherichia coli; allergenicity; atomic force microscopy; cattle; cheesemaking; cheeses; chymosin; cysteine proteinases; embryo (animal); kappa-casein; latex; metal ions; micelles; peptides; sensory evaluation; water content
- ... This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by ...
- Silva, Ayrles F.B., et al. Show all 7 Authors
- Journal of ethnopharmacology 2010 v.129 no.3 pp. 327-334
- Salmonella Typhimurium; medicinal plants; microbial growth; intraperitoneal injection; oral administration; protective effect; plant proteins; spleen; medicinal properties; bacterial infections; herbal medicines; antimicrobial properties; septic shock; animal pathogenic bacteria; Calotropis procera; mice; subcutaneous injection; histopathology; mortality; liver; inflammation; animal models; latex; signs and symptoms (animals and humans)
- ... Aim of the study: The latex of Calotropis procera has been used in traditional medicine to treat different inflammatory diseases. The anti-inflammatory activity of latex proteins (LP) has been well documented using different inflammatory models. In this work the anti-inflammatory protein fraction was evaluated in a true inflammatory process by inducing a lethal experimental infection in the murine ...