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1. Genome-Wide Identification and Analysis of Biotic and Abiotic Stress Regulation of C4 Photosynthetic Pathway Genes in Rice

Author:
Singh, Ashok K.,   et al.   ; Muthusamy, Senthilkumar K.; Lenka, Sangram K.; Katiyar, Amit; Chinnusamy, Viswanathan; Bansal, Kailash C.;     Show all 6 Authors
Source:
Applied biochemistry and biotechnology 2019 v.187 no.1 pp. 221-238
ISSN:
0273-2289
Subject:
Brachypodium; C3 plants; C4 photosynthesis; Oryza australiensis; Oryza barthii; Oryza sativa; Sorghum (Poaceae); abiotic stress; biotic stress; breeding; carbon dioxide; corn; developmental stages; enzymes; gene expression regulation; genes; genetic engineering; genotype; grasses; leaves; phylogeny; quantitative analysis; rice; tillering
Abstract:
... Photosynthetic fixation of CO₂ is more efficient in C₄ than in C₃ plants. Rice is a C₃ plant and a potential target for genetic engineering of the C₄ pathway. It is known that genes encoding C₄ enzymes are present in C₃ plants. However, no systematic analysis has been conducted to determine if these C₄ gene family members are expressed in diverse rice genotypes. In this study, we identified 15 gen ...
DOI:
10.1007/s12010-018-2809-0

2. Betulinic acid as apoptosis activator: Molecular mechanisms, mathematical modeling and chemical modifications

Author:
Singh, Ashok K.,   et al.   ; Kumar, Pranesh; Bhadauria, Archana S.; Saha, Sudipta;     Show all 4 Authors
Source:
Life sciences 2018 v.209 pp. 24-33
ISSN:
0024-3205
Subject:
apoptosis; betulinic acid; clinical trials; cytotoxicity; drug therapy; mathematical models; mitochondria; neoplasm cells; skin neoplasms; tissues
Abstract:
... A natural product betulinic acid (BA) has gained a huge significance in the recent years for its strong cytotoxicity. Surprisingly, in spite of being an interesting cancer protecting agent on a variety of tumor cells, the normal cells and tissues are rarely affected by BA. Betulinic acid and analogues (BAs) generally exert through the mechanisms that provokes an event of direct cell death and bypa ...
DOI:
10.1016/j.lfs.2018.07.056

3. Pore Characteristics of Distinct Thermally Mature Shales: Influence of Particle Size on Low-Pressure CO2 and N2 Adsorption

Author:
Singh, Ashok K.,   et al.   ; Hazra, Bodhisatwa; Wood, David A.; Vishal, Vikram;     Show all 4 Authors
Source:
Energy & fuels 2018 v.32 no.8 pp. 8175-8186
ISSN:
1520-5029
Subject:
adsorption; carbon dioxide; desorption; fractal dimensions; fuels; nanopores; nitrogen; oils; particle size; shale; shale gas
Abstract:
... The influence of crushed sample particle size on low-pressure gas adsorption and desorption behavior of shales and their measurement is an issue of significant current interest in this new era focused on shale gas and oil resources. Here we study two samples of distinct Indian shales, with different organic contents, ages, levels of thermal maturity, and pore-size distributions crushed to four dif ...
DOI:
10.1021/acs.energyfuels.8b01439

4. Genetic engineering of indica rice with AtDREB1A gene for enhanced abiotic stress tolerance

Author:
Singh, Ashok K.,   et al.   ; Latha, Gunturu Manju; Raman, K. V.; Lima, John Milton; Pattanayak, Debasis; Chinnusamy, Viswanathan; Bansal, Kailash C.; Sambasiva Rao, K. R. S.; Mohapatra, Trilochan;     Show all 9 Authors
Source:
Plant cell, tissue, and organ culture 2019 v.136 no.1 pp. 173-188
ISSN:
0167-6857
Subject:
Oryza sativa; Southern blotting; canopy; carbon; chlorophyll; chloroplasts; cold; cold stress; cold tolerance; drought; drought tolerance; gene expression regulation; gene overexpression; genetic engineering; leaves; microarray technology; proteins; regulatory sequences; reverse transcriptase polymerase chain reaction; rice; staple crops; stress tolerance; sugars; temperature; transcription (genetics); transgenes; transgenic plants; water content; water stress
Abstract:
... Drought and cold stresses are major abiotic stresses that affect rice productivity. Therefore, enhancing tolerance to these stresses is necessary for sustaining rice productivity. Overexpression of the AtDREB1A gene has been shown to confer tolerance to both drought and cold stresses in diverse plant species. Hence, we genetically engineered indica rice cv. Pusa Sugandh 2 with AtDREB1A gene under ...
DOI:
10.1007/s11240-018-1505-7

5. Transcriptional signatures modulating shoot apical meristem morphometric and plant architectural traits enhance yield and productivity in chickpea

Author:
Singh, Ashok K.,   et al.   ; Narnoliya, Laxmi; Basu, Udita; Bajaj, Deepak; Malik, Naveen; Thakro, Virevol; Daware, Anurag; Sharma, Akash; Tripathi, Shailesh; Hegde, Venkatraman S.; Upadhyaya, Hari D.; Tyagi, Akhilesh K.; Parida, Swarup K.;     Show all 13 Authors
Source:
Theplant journal 2019 v.98 no.5 pp. 864-883
ISSN:
0960-7412
Subject:
agronomic traits; alleles; apical meristems; binding sites; chickpeas; crops; cultivars; farmers; food security; gene expression regulation; genetic improvement; genome-wide association study; genomics; haplotypes; introgression; isogenic lines; land use; messenger RNA; molecular cloning; morphometry; phenotype; plant density; plant height; planting density; population growth; quantitative trait loci; stem cells; transcription (genetics); transcription factors
Abstract:
... Plant height (PH) and plant width (PW), two of the major plant architectural traits determining the yield and productivity of a crop, are defined by diverse morphometric characteristics of the shoot apical meristem (SAM). The identification of potential molecular tags from a single gene that simultaneously modulates these plant/SAM architectural traits is therefore prerequisite to achieve enhanced ...
DOI:
10.1111/tpj.14284

6. Aerobic and Anaerobic Transformations in Estrogens and Nutrients in Swine Manure: Environmental Consequences

Author:
Singh, Ashok K.
Source:
Agriculture 2015 v.5 no.3 pp. 697-712
ISSN:
2077-0472
Subject:
ammonia; ammonium; conjugated estrogens; estradiol; estriol; estrogenic properties; estrone; hydrolysis; mineralization; nitrates; nutrients; organic carbon; organic fertilizers; organic nitrogen; pig manure; total nitrogen; toxicity
Abstract:
... Pig manure is an excellent fertilizer and rich source of organic carbon and nitrogen compounds such organic nitrogen (O-N) (95% of total nitrogen) that is plant-unavailable-nitrogen (PUN) and mineralized nitrogen (about 1% of total nitrogen) such as ammonium (NH₄⁺) and nitrate (NO₃) that are plant-available-nitrogen (PAN). In addition, manure also contains two forms of estrogens: (i) poorly estrog ...
DOI:
10.3390/agriculture5030697

7. Allele‐specific analysis of single parent backcross population identifies HOX10 transcription factor as a candidate gene regulating rice root growth

Author:
Singh, Ashok K.,   et al.   ; Reddy, Sowmya H.; Kambalimath, Sumanth K.; Singhal, Rajesh K.; Chikkakariyappa, Manjunath K.; Muthurajan, Raveendran; Rajanna, Mavinahalli P.; Sreevathsa, Rohini; Sevanthi, Amitha M.; Mohapatra, Trilochan; Sarla, Neelamraju; Chinnusamy, Viswanathan; Krishnan, Gopala S.; Singh, Nagendra K.; Sharma, Rameshwar P.; Sheshshayee, Sreeman M.;     Show all 16 Authors
Source:
Physiologia plantarum 2019 v.166 no.2 pp. 596-611
ISSN:
0031-9317
Subject:
alleles; backcrossing; biomass; carbon; carbon dioxide fixation; citrate (si)-synthase; drought; genetic variation; leaves; methane; mutants; mutation; phenotype; progeny; rice; root growth; screening; stable isotopes; transcription factors; turgor; zeaxanthin epoxidase
Abstract:
... Understanding the molecular and physiological mechanisms of trait diversity is crucial for crop improvement to achieve drought adaptation. Root traits such as high biomass and/or deep rootedness are undoubtedly important drought adaptive traits. The major aim of this investigation was to functionally characterize a set of ethyl methane sulfonate‐induced rice mutants for root traits. We report the ...
DOI:
10.1111/ppl.12826

8. Potentiometric sensor for the nanoscale monitoring of Ni2+ ion in environmental samples by the fabrication of coated pyrolytic graphite electrode based on a novel C–C-coupled compound

Author:
Singh, Ashok K.,   et al.   ; Sahani, Manoj K.; Singhal, Divya; Jain, Ajay K.; Singh, Udai P.; Narang, Shikha;     Show all 6 Authors
Source:
International journal of environmental analytical chemistry 2016 v.96 no.12 pp. 1170-1187
ISSN:
1029-0397
Subject:
EDTA (chelating agent); acetonitrile; compliance; detection limit; electrodes; graphene; ligands; methanol; monitoring; nickel; pH; poly(vinyl chloride); solvents; titration; vinyl chloride
Abstract:
... Novel ligand 5,5ʹ-((3-nitrophenyl)methylene)bis(2,6-diaminopyrimidin-4(3H)-one) (L) was synthesised and characterised. Preliminary studies on L have showed that it has more affinity towards the Ni ²⁺ ion. Thus, the L was used as the electroactive material in the fabrication of poly(vinyl chloride) (PVC)-based membrane sensors such as coated graphite electrode (CGE) and coated pyrolytic graphite el ...
DOI:
10.1080/03067319.2016.1244671

9. Development and validation of co-dominant gene based markers for Pi9, a gene governing broad-spectrum resistance against blast disease in rice

Author:
Singh, Ashok K.,   et al.   ; Rathour, R.; Gopala Krishnan, S.; Khanna, Apurva; Dhatwalia, Swaranjali; Kaachra, A.; Sharma, T. R.;     Show all 7 Authors
Source:
Molecular breeding 2016 v.36 no.12 pp. 168
ISSN:
1380-3743
Subject:
Magnaporthe oryzae; Oryza minuta; Oryza sativa; blast disease; breeding programs; crop production; cultivars; disease resistance; fungi; genes; genetic resistance; grain quality; marker-assisted selection; rice
Abstract:
... Rice production and grain quality are severely affected by blast disease caused by the ascomycetous fungus Magnaporthe oryzae. Incorporation of genes that confer broad-spectrum resistance to blast has been a priority area in rice breeding programs. The blast resistance gene Pi9 sourced from Oryza minuta has shown broad spectrum and durable resistance to blast world-wide. In the present study co-do ...
DOI:
10.1007/s11032-016-0599-z

10. Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells

Author:
Singh, Ashok K.,   et al.   ; Kumar, Pranesh; Rawat, Atul; Keshari, Amit K.; Maity, Siddhartha; De, Arnab; Samanta, Amalesh; Saha, Sudipta;     Show all 8 Authors
Source:
Natural product research 2016 v.30 no.4 pp. 460-463
ISSN:
1478-6427
Subject:
Mucuna pruriens; alkaloids; apoptosis; binding capacity; caspase-3; caspase-8; cell growth; cytotoxicity; growth retardation; humans; liver neoplasms; models; neoplasm cells; seeds
Abstract:
... The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell ...
DOI:
10.1080/14786419.2015.1020489

11. Introgression of Pi2 and Pi5 Genes for Blast (Magnaporthe oryzae) Resistance in Rice and Field Evaluation of Introgression Lines for Resistance and Yield Traits

Author:
Singh, Ashok K.,   et al.   ; Sagar KrishnaMurthy, Prashanthi; Deshmukh, Dnyaneshwar B.; Yashvanth Kumar, Kakanur Jagadeesh; Patil, Santosh; Jakkeral, Shridevi; Nemappa, Gangappa Hanamaratti; Singh, Uday D.; Variar, Mukund; Rathour, Rajeev; Subbaiyan, Gopalakrishnan; Sharma, Tilak Raj;     Show all 12 Authors
Source:
Phytopathologische Zeitschrift 2017 v.165 no.6 pp. 397-405
ISSN:
0931-1785
Subject:
Magnaporthe oryzae; backcrossing; blast disease; genes; genetic resistance; grain yield; homozygosity; introgression; leaves; rice
Abstract:
... Blast caused by Magnaporthe oryzae is the most devastating disease causing significant loss in rice production. The destructive nature of the disease is mainly due to the genetic plasticity of M. oryzae which complicates the breeding strategies. Blast can be effectively managed by the deployment of R genes. In this study, broad‐spectrum blast resistance genes Pi2 and Pi5 were introgressed independ ...
DOI:
10.1111/jph.12573

12. Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice

Author:
Singh, Ashok K.,   et al.   ; Fiyaz, R. Abdul; Yadav, Ashutosh K.; Krishnan, S. Gopala; Ellur, Ranjith K.; Bashyal, Bishnu M.; Grover, Nitasha; Bhowmick, Prolay K.; Nagarajan, M.; Vinod, K. K.; Singh, Nagendra K.; Prabhu, Kumble V.;     Show all 12 Authors
Source:
Rice 2016 v.9 no.1 pp. 45
ISSN:
1939-8425
Subject:
Fusarium fujikuroi; breeding; chromosome mapping; cultivars; emerging diseases; genes; inbred lines; parents; phenotypic variation; quantitative trait loci; rice; screening; seedlings; teleomorphs
Abstract:
... BACKGROUND: Bakanae or foot rot disease caused by Fusarium fujikuroi [teleomorph: Gibberella fujikuroi (Sawada) Ito] is emerging as a serious disease in rice. The disease causes both quantitative and qualitative losses to the grains under the field conditions. Breeding for resistance to Bakanae disease is a promising strategy to manage this emerging disease. In this study, we used a population of ...
DOI:
10.1186/s12284-016-0117-2
PubMed:
27620639
PubMed Central:
PMC5019990

13. Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding

Author:
Singh, Ashok K.,   et al.   ; Ellur, Ranjith K.; Khanna, Apurva; Yadav, Ashutosh; Pathania, Sandeep; Rajashekara, H.; Singh, Vikas K.; Gopala Krishnan, S.; Bhowmick, Prolay K.; Nagarajan, M.; Vinod, K.K.; Prakash, G.; Mondal, Kalyan K.; Singh, Nagendra K.; Vinod Prabhu, K.;     Show all 15 Authors
Source:
Plant science 2016 v.242 pp. 330-341
ISSN:
0168-9452
Subject:
Magnaporthe oryzae; Oryza sativa; Xanthomonas oryzae pv. oryzae; backcrossing; blast disease; blight; disease resistance; early development; genes; genetic background; genetic markers; microsatellite repeats; parents; phenotype; plant breeding; races; rice; single nucleotide polymorphism; India
Abstract:
... Marker assisted backcross breeding was employed to incorporate the blast resistance genes, Pi2 and Pi54 and bacterial blight (BB) resistance genes xa13 and Xa21 into the genetic background of Pusa Basmati 1121 (PB1121) and Pusa Basmati 6. Foreground selection for target gene(s) was followed by arduous phenotypic and background selection which fast-tracked the recovery of recurrent parent genome (R ...
DOI:
10.1016/j.plantsci.2015.08.020

14. Antidiabetic effects of isolated sterols from Ficus racemosa leaves

Author:
Singh, Ashok K.,   et al.   ; Kushwaha, Priya Singh; Raj, Vinit; Keshari, Amit K.; Saraf, Shubhini A.; Mandal, Subhash C.; Yadav, Rajnish Kumar; Saha, Sudipta;     Show all 8 Authors
Source:
RSC advances 2015 v.5 no.44 pp. 35230-35237
ISSN:
2046-2069
Subject:
Ficus racemosa; albino; animal disease models; antioxidant activity; antioxidants; biomarkers; blood glucose; blood lipids; body weight changes; diabetes; drug design; enzymes; glutathione; glycemic effect; high density lipoprotein; leaves; lipid composition; liver; oral administration; oxidative stress; pancreas; rats; sterols; streptozotocin; toxicity
Abstract:
... The present study was undertaken to evaluate the antidiabetic, hypolipidemic, and toxic effects of isolated sterols from Ficus racemosa (FR) leaves using streptozotocin induced diabetic rats. Diabetes was induced by the administration of streptozotocin (50 mg kg⁻¹) intraperitoneally to albino rats. Three sterols were administered once a day for a period of seven days at a dose of 100 mg kg⁻¹ body ...
DOI:
10.1039/c5ra00790a

15. Reduced heterozygosity at intragenic and flanking microsatellites of pfcrt gene establishes natural selection based molecular evolution of chloroquine-resistant Plasmodium falciparum in India

Author:
Singh, Ashok K.,   et al.   ; Mallick, Prashant K.; Singh, Ruchi; Singh, Om P.; Bhasin, Virendra K.; Valecha, Neena;     Show all 6 Authors
Source:
Infection, genetics, and evolution 2013 v.20 pp. 407-412
ISSN:
1567-1348
Subject:
Plasmodium falciparum; parasites; microsatellite repeats; loci; t-test; genetic variation; models; mutation; heterozygosity; mutants; natural selection; chloroquine; exons; genetic recombination; India
Abstract:
... The positive selection of a nucleotide substitution in exon 2 of Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene (mutation responsible for chloroquine resistance) causes a reduction in variation of neutral loci close to the gene. This reduction in allelic diversity around flanking regions of pfcrt gene was reported in worldwide chloroquine resistant isolates and referred as s ...
DOI:
10.1016/j.meegid.2013.10.001
PubMed:
24126360

16. Anti-Inflammatory Potency of Nano-Formulated Puerarin and Curcumin in Rats Subjected to the Lipopolysaccharide-Induced Inflammation

Author:
Singh, Ashok K.,   et al.   ; Jiang, Yin; Gupta, Shveta; Younus, Mohamod; Ramzan, Mohamod;     Show all 5 Authors
Source:
Journal of medicinal food 2013 v.16 no.10 pp. 899-911
ISSN:
1557-7600
Subject:
Ayurvedic medicine; Oriental traditional medicine; active ingredients; adverse effects; animal models; apoptosis; bioavailability; blood; brain; clinical trials; curcumin; cytotoxicity; inflammation; lactic acid; lipopolysaccharides; nanogold; nanoparticles; rats; renal function
Abstract:
... Puerarin (PU) and curcumin (CU), used commonly in traditional Chinese medicine and Ayurveda, have been shown to possess potent anti-inflammatory, anti-oxidation, and neuro-protective properties. Despite the experimental success of CU and PU in in vitro and animal models, their effectiveness has not yet been demonstrated in clinical trials, possibly because of their poor bioavailability. We hypothe ...
DOI:
10.1089/jmf.2012.0049

17. Quantitative analysis of conjugated and free estrogens in swine manure: Solutions to overcome analytical problems due to matrix effects

Author:
Singh, Ashok K.,   et al.   ; Gupta, Shveta; Kumar, Kuldip; Gupta, Satish; Chander, Yogesh; Gupta, Arun; Saxena, Richa;     Show all 7 Authors
Source:
Journal of chromatography 2013 v.1305 pp. 203-212
ISSN:
0021-9673
Subject:
chromatography; conjugated estrogens; deuterium; ethyl acetate; farms; methanol; pig manure; quantitative analysis; swine
Abstract:
... Although animal manure is an important source for environmental estrogens, quantitative analysis of estrogens in manure is complicated due to matrix interference. In the present study, chromatographic methods have been developed for quantification of conjugated and free estrogens in manure samples collected from pig farms. The whole manure samples, immediately after collection, were stored at 4°C, ...
DOI:
10.1016/j.chroma.2013.06.026

18. Electroanalytical performance of Cd(II) selective sensor based on PVC membranes of 5,5′‐(5,5′‐(benzo[c][1,2,5]thiadiazole‐4,7‐diyl)bis(thiophene‐5,2‐diyl))bis(N1,N1,N3,N3‐tetraphenylbenzene‐1,3‐diamine)

Author:
Singh, Ashok K.,   et al.   ; Jain, A.K.; Upadhyay, Anjali; Thomas, K.R.J.; Singh, Prerna;     Show all 5 Authors
Source:
International journal of environmental analytical chemistry 2013 v.93 no.8 pp. 813-827
ISSN:
1029-0397
Subject:
EDTA (chelating agent); additives; cadmium; detection limit; electrodes; ions; medicinal plants; pH; potassium; protons; shelf life; sodium; soil sampling; titration
Abstract:
... The construction and performance characteristics of polymeric membrane electrodes based on neutral ionophore 5,5′‐(5,5′‐(benzo[c][1,2,5]thiadiazole‐4,7‐diyl)bis(thiophene‐5,2‐diyl))bis‐(N1,N1,N3,N3‐tetraphenylbenzene‐1,3‐diamine) (L) for quantification of cadmium ions, are described. Effect of plastisizers dibutylpthalate (DBP), tri‐n‐butylphosphate (TBP), dioctylpthalate (DOP), o‐nitrophenyloctyl ...
DOI:
10.1080/03067319.2011.649744

19. Development of a coupling-phase SCAR marker linked to the powdery mildew resistance gene ‘er1’ in pea (Pisum sativum L.)

Author:
Singh, Ashok K.,   et al.   ; Srivastava, Rakesh K.; Mishra, Shashi K.; Mohapatra, Trilochan;     Show all 4 Authors
Source:
Euphytica 2012 v.186 no.3 pp. 855-866
ISSN:
0014-2336
Subject:
DNA; Pisum sativum; cultivars; marker-assisted selection; peas; powdery mildew; random amplified polymorphic DNA technique; recessive genes; seed yield
Abstract:
... Pea powdery mildew is one of the major constraints in pea production worldwide, causing severe seed yield and quality loss. The resistance is governed by a single recessive gene er1 in majority of resistant cultivars, but er2 and Er3 have also been reported. The objective of the study was to find out tightly linked sequence characterized amplified regions (SCAR) markers to er1 gene using NILs. A t ...
DOI:
10.1007/s10681-012-0650-z

20. Introgression of multiple disease resistance into a maintainer of Basmati rice CMS line by marker assisted backcross breeding

Author:
Singh, Ashok K.,   et al.   ; Singh, Vikas K.; Singh, Atul; Ellur, Ranjith K.; Pandian, R. T. P.; Gopala Krishnan, S.; Singh, U. D.; Nagarajan, M.; Vinod, K. K.; Prabhu, K. V.;     Show all 10 Authors
Source:
Euphytica 2015 v.203 no.1 pp. 97-107
ISSN:
0014-2336
Subject:
Magnaporthe oryzae; Oryza sativa; Thanatephorus cucumeris; alleles; backcrossing; blast disease; blight; cooking quality; cultivars; disease resistance; fungi; heterozygosity; homozygosity; hybrids; introgression; loci; plant breeding; quantitative trait loci; restorer lines; rice; screening; selfing; virulent strains
Abstract:
... Globally, blast incited by Magnaporthe oryzae and sheath blight (ShB) by Rhizoctonia solani Kuhn forms two major fungal diseases that cause significant yield loss in rice. Pusa 6B, the Basmati quality maintainer line of the popular superfine grain aromatic rice hybrid Pusa RH10, is highly susceptible to both the diseases. The rice cultivar Tetep was used as the donor for transferring a major blast ...
DOI:
10.1007/s10681-014-1267-1