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- Siriwardena, Sachini
U., et al. Show all 3 Authors
- Chemical Reviews 2016 v.116 no.20 pp. 12688-12710
- Human immunodeficiency virus 1; antibodies; carcinogenesis; cell cycle; cytokines; enzymes; genes; humans; inflammation; lipids; messenger RNA; mutagens; mutation; physiological state; retrotransposons; single-stranded DNA; transcription (genetics); viruses
- ... The AID/APOBEC family enzymes convert cytosines in single-stranded DNA to uracils, causing base substitutions and strand breaks. They are induced by cytokines produced during the body’s inflammatory response to infections, and they help combat infections through diverse mechanisms. AID is essential for the maturation of antibodies and causes mutations and deletions in antibody genes through somati ...
- PubMed Central:
- Siriwardena, Sachini U., et al. Show all 3 Authors
- Journal of Molecular Biology 2015 v.427 pp. 3042-3055
- DNA; Escherichia coli; active sites; adaptive immunity; deamination; desorption; enzymes; gel chromatography; genes; humans; methionine; molecular models; mutagenicity; mutants; mutation; neoplasms; proteins; substrate specificity; thymine
- ... Human APOBEC3B deaminates cytosines in DNA and belongs to the AID/APOBEC family of enzymes. These proteins are involved in innate and adaptive immunity and may cause mutations in a variety of cancers. To characterize its ability to convert cytosines into uracils, we tested several derivatives of APOBEC3B gene for their ability to cause mutations in Escherichia coli. Through this analysis, a methio ...
- PubMed Central:
- Siriwardena, Sachini U., et al. Show all 18 Authors
- Cell 2019 v.177 no.4 pp. 1067-1079.e19
- CRISPR-Cas systems; Streptococcus pyogenes; gene editing; nucleases; pharmacology; screening; structure-activity relationships; transcriptional activation
- ... The precise control of CRISPR-Cas9 activity is required for a number of genome engineering technologies. Here, we report a generalizable platform that provided the first synthetic small-molecule inhibitors of Streptococcus pyogenes Cas9 (SpCas9) that weigh <500 Da and are cell permeable, reversible, and stable under physiological conditions. We developed a suite of high-throughput assays for SpCas ...