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1. Development of Real-Time and Conventional PCR Assays for Identifying Stubby Root Nematode Paratrichodorus allius

Author:
Skantar, Andrea M.,   et al.   ; Huang, Danqiong; Yan, Guiping;     Show all 3 Authors
Source:
Plant disease 2017 v.101 no.6 pp. 964-972
ISSN:
0191-2917
Subject:
Paratrichodorus; Tobacco rattle virus; crops; detection limit; disease control; internal transcribed spacers; pests; potatoes; quantitative polymerase chain reaction; ribosomal DNA; soil sampling; species identification; tubers; Minnesota; North Dakota
Abstract:
... Paratrichodorus allius is an important pest on many crops, particularly on potato due to its ability to transmit Tobacco rattle virus causing corky ringspot disease on tubers. Detection and identification of P. allius are important for effective disease management. In this study, a rapid and reliable molecular diagnosis of this nematode targeting internal transcribed spacer ribosomal DNA was estab ...
DOI:
10.1094/PDIS-10-16-1431-RE

2. Developing a Real-Time PCR Assay for Direct Identification and Quantification of Pratylenchus penetrans in Soil

Author:
Skantar, Andrea M.,   et al.   ; Baidoo, Richard; Yan, Guiping; Nagachandrabose, Seenivasan;     Show all 4 Authors
Source:
Plant disease 2017 v.101 no.8 pp. 1432-1441
ISSN:
0191-2917
Subject:
Pratylenchus penetrans; Verticillium dahliae; control methods; diagnostic techniques; extension education; fungi; grinding; pathogens; pest control; planting; potatoes; quantitative polymerase chain reaction; ribosomal DNA; root lesion nematodes; soil; taxonomy
Abstract:
... The root-lesion nematode Pratylenchus penetrans is a major pathogen of potato worldwide. Yield losses may be exacerbated by interaction with the fungus Verticillium dahliae in the potato early dying disease complex. Accurate identification and quantification of P. penetrans prior to planting are essential for developing effective integrated pest control measures. However, distinction between P. pe ...
DOI:
10.1094/PDIS-01-17-0117-RE

3. A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil

Author:
Skantar, Andrea M.,   et al.   ; Sapkota, Rumakanta; Nicolaisen, Mogens;     Show all 3 Authors
Source:
Nematology 2016 v.18 no.2 pp. 147-154
ISSN:
1388-5545
Subject:
DNA; DNA primers; Daucus carota; Meloidogyne hapla; carrots; disease control; eggs; planting; quantitative polymerase chain reaction; root galls; soil
Abstract:
... Early detection and quantification of Meloidogyne hapla in soil is essential for effective disease management. The purpose of this study was to develop a real-time PCR assay for detection of M. hapla in soil. Primers and a TaqMan probe were designed for M. hapla detection. The assay detected M. hapla and showed no significant amplification of DNA from non-target nematodes. The assay was able to de ...
Handle:
10113/62017
DOI:
10.1163/15685411-00002950

4. Use of Chemical Flocculation and Nested PCR for Heterodera glycines Detection in DNA Extracts from Field Soils with Low Population Densities

Author:
Skantar, Andrea M.,   et al.   ; Baidoo, Richard; Yan, Guiping; Nelson, Berlin; Chen, Senyu;     Show all 5 Authors
Source:
Plant disease 2017 v.101 no.7 pp. 1153-1161
ISSN:
0191-2917
Subject:
DNA; DNA primers; Heterodera glycines; eggs; flocculation; grinding; juveniles; pathogens; polymerase chain reaction; population density; soil; soil sampling; soil sterilization; soybeans; sulfates
Abstract:
... The soybean cyst nematode (SCN) Heterodera glycines is a major pathogen of soybean worldwide. Distinction between SCN and other members of the H. schachtii sensu stricto group based on morphology is a tedious task. A molecular assay was developed to detect SCN in field soils with low population densities and to differentiate SCN from other species. Various numbers of SCN eggs or juveniles were ino ...
DOI:
10.1094/PDIS-08-16-1163-RE

5. Real-Time PCR for Detection and Identification of Anguina funesta, A. agrostis, A. tritici, and A. pacificae

Author:
Skantar, Andrea M.,   et al.   ; Li, Wenbin; Yan, Zonghe; Nakhla, Mark K.;     Show all 4 Authors
Source:
Plant disease 2015 v.99 no.11 pp. 1584-1589
ISSN:
0191-2917
Subject:
Agrostis capillaris; Anguina tritici; Rathayibacter toxicus; forage; grasses; leaves; plant parasitic nematodes; quantitative polymerase chain reaction; ribosomal DNA; sequence diversity; stem galls; toxigenic strains; wheat
Abstract:
... A number of seed, leaf, and stem gall nematodes are of significance to the forage and landscape grass and livestock industries. In North America, the bentgrass nematode, Anguina agrostis, reduces seed production on Agrostis tenuis and several other grass species. Anguina funesta is a seed-gall nematode that is most significant for its association with the toxigenic bacteria Rathayibacter toxicus. ...
Handle:
10113/62030
DOI:
10.1094/PDIS-09-14-0959-RE

6. Assessment of DAPG-producing Pseudomonas fluorescens for Management of Meloidogyne incognita and Fusarium oxysporum on Watermelon

Author:
Skantar, Andrea M.,   et al.   ; Meyer, Susan L. F.; Everts, Kathryne L.; McSpadden Gardener, Brian; Masler, Edward P.; Abdelnabby, Hazem M. E.;     Show all 6 Authors
Source:
Journal of nematology 2016 v.48 no.1 pp. 43-53
ISSN:
0022-300X
Subject:
Citrullus lanatus var. lanatus; Fusarium oxysporum f. sp. niveum; Meloidogyne incognita; Pseudomonas fluorescens; antibiotics; beneficial microorganisms; biological control; death; eggs; fruit growing; greenhouse experimentation; greenhouses; juveniles; nematode control; nematode infections; plant growth; root-knot nematodes; soil; soil-borne diseases; vigor; vines; watermelons; wood; Maryland
Abstract:
... Pseudomonas fluorescens isolates Clinto 1R, Wayne 1R and Wood 1R, which produce the antibiotic 2,4-diacetylphloroglucinol (DAPG), can suppress soilborne diseases and promote plant growth. Consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of Meloidogyne incognita (root-knot nematode: RKN) and Fusarium oxysporum f. sp. niveum (Fon). In a greenhouse tr ...
Handle:
10113/62646

7. A new chorismate mutase gene identified from Globodera ellingtonae and its utility as a molecular diagnostic marker

Author:
Skantar, Andrea M.,   et al.   ; Chronis, Demosthenis; Chen, Shiyan; Zasada, Inga A.; Wang, Xiaohong;     Show all 5 Authors
Source:
European journal of plant pathology 2014 v.139 no.2 pp. 245-252
ISSN:
0929-1873
Subject:
DNA primers; Globodera; alternative splicing; cyst nematodes; diagnostic techniques; genes; messenger RNA; potatoes; quantitative polymerase chain reaction; sequence analysis; United States
Abstract:
... Globodera ellingtonae, a new cyst nematode species recently detected in Oregon and confirmed to reproduce on potato, shares key morphological features with the two species of potato cyst nematode (PCN; G. rostochiensis and G. pallida) which are of quarantine concern. Currently no methods are available for the molecular diagnosis of this new Globodera species. In this study, we cloned a chorismate ...
DOI:
10.1007/s10658-014-0385-x

8. Detection of Rhynchophorus palmarum (Coleoptera: Curculionidae) and Identification of Associated Nematodes in South Texas

Author:
Skantar, Andrea M.,   et al.   ; Esparza-Díaz, Gabriela; Olguin, Alma; Carta, Lynn K.; Villanueva, Raul T.;     Show all 5 Authors
Source:
The Florida entomologist 2013 v.96 no.4 pp. 1513-1521
ISSN:
0015-4040
Subject:
Arecaceae; Bursaphelenchus; Rhynchophorus ferrugineus; Rhynchophorus palmarum; coconuts; ethyl acetate; ethylene glycol; insects; invasive species; kairomones; nematode larvae; sugars; surveys; traps; Mexico; Texas
Abstract:
... This study reports a survey conducted to find the South American palm weevil Rhynchophorus palmarum (L.) and the red palm weevil R. ferrugineus (Olivier) (Coleoptera: Curculionidae), 2 invasive species of palm trees. The study was performed in the Rio Grande Valley of south Texas and near the border with Tamaulipas state, Mexico. A total of 40 traps were inspected biweekly from 26 Sep 2011 to 20 S ...
DOI:
10.1653/024.096.0433

9. Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae

Author:
Skantar, Andrea M.,   et al.   ; Yan, Guiping; Smiley, Richard W.; Okubara, Patricia A.;     Show all 4 Authors
Source:
Plant disease 2013 v.97 no.12 pp. 1611-1619
ISSN:
0191-2917
Subject:
Globodera; Heterodera avenae; Heterodera filipjevi; Meloidogyne; Pratylenchus; cyst nematodes; eggs; grain crops; internal transcribed spacers; juveniles; polymerase chain reaction; ribosomal DNA; Northwestern United States
Abstract:
... Heterodera avenae and H. filipjevi are economically important cyst nematodes that restrict production of cereal crops in the Pacific Northwest United States and elsewhere in the world. Identification of these two species is critical for recommending and implementing effective management practices. Primers were designed from the internal transcribed spacer (ITS) regions of H. avenae and H. filipjev ...
DOI:
10.1094/PDIS-01-13-0064-RE

10. Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil

Author:
Skantar, Andrea M.,   et al.   ; Yan, Guiping; Smiley, Richard W.; Okubara, Patricia A.; Reardon, Catherine L.;     Show all 5 Authors
Source:
Plant disease 2013 v.97 no.6 pp. 757
ISSN:
0191-2917
Subject:
DNA primers; Pratylenchus neglectus; bioassays; crop rotation; cultivars; detection; internal transcribed spacers; microscopy; plant parasitic nematodes; polymerase chain reaction; quantitative analysis; ribosomal DNA; roots; soil; soil sterilization; wheat; Pacific States
Abstract:
... Pratylenchus neglectus is one of the most widespread and economically important nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest. It is challenging to quantify P. neglectus using microscopic methods for studies that require large-scale sampling, such as assessment of rotation crops, wheat cultivars, and other management practices. A real-time quantitativ ...
Handle:
10113/56556
DOI:
10.1094/PDIS-08-12-0729-RE

11. Morphological and molecular observations on the cereal cyst nematode Heterodera filipjevi from the Middle Volga River and South Ural Regions of Russia

Author:
Skantar, Andrea M.,   et al.   ; Pridannikov, Mikhail V.; Suprunova, Tatiana P.; Shumilina, Daria V.; Limantseva, Lyudmila A.; Handoo, Zafar A.; Chitwood, David J.;     Show all 7 Authors
Source:
Russian journal of nematology 2015 v.23 no.2 pp. 113-124
ISSN:
0869-6918
Subject:
DNA; Heterodera; animal morphology; cyst nematodes; internal transcribed spacers; juveniles; molecular systematics; morphometry; nematology; pharynx; polymerase chain reaction; population; population density; rhizosphere; ribosomal RNA; rivers; soil; soil nematodes; soil sampling; species diversity; surveys; zoogeography; Russia
Abstract:
... During 2010-2012, a survey was conducted to determine the distribution and species diversity of the cereal cyst nematode Heterodera filipjevi within the Volga and South Ural regions of the Russian Federation. A total of 270 soil samples were collected. Seven populations of CCN were found in the rhizosphere of various cereal plants that showed symptoms of nematode disease in Saratov and Chelyabinsk ...

12. Root-Knot Nematodes in Golf Course Greens of the Western United States

Author:
Skantar, Andrea M.,   et al.   ; McClure, Michael A.; Nischwitz, Claudia; Schmitt, Mark E.; Subbotin, Sergei A.;     Show all 5 Authors
Source:
Plant disease 2012 v.96 no.5 pp. 635-647
ISSN:
0191-2917
Subject:
Meloidogyne naasi; cytochrome-c oxidase; genes; golf courses; intergenic DNA; lawns and turf; mitochondrial DNA; mitochondrial genome; nucleotide sequences; phylogeny; polymerase chain reaction; restriction endonucleases; restriction fragment length polymorphism; ribosomal RNA; root-knot nematodes; surveys; Arizona; California; Hawaii; Nevada; Utah; Washington (state)
Abstract:
... A survey of 238 golf courses in 10 states of the western United States found root-knot nematodes (Meloidogyne spp.) in 60% of the putting greens sampled. Sequence and phylogenetic analyses of 18S rRNA, D2-D3 of 28S rRNA, internal transcribed spacer-rRNA, and mitochondrial DNA gene sequences were used to identify specimens from 110 golf courses. The most common species, Meloidogyne naasi, was found ...
DOI:
10.1094/PDIS-09-11-0808

13. Description of Globodera ellingtonae n. sp. (Nematoda: Heteroderidae) from Oregon

Author:
Skantar, Andrea M.,   et al.   ; Handoo, Zafar A.; Carta, Lynn K.; Chitwood, David J.;     Show all 4 Authors
Source:
Journal of nematology 2012 v.44 no.1 pp. 40
ISSN:
0022-300X
Subject:
Globodera; anus; body length; cyst nematodes; descriptions; enzyme polymorphism; females; head; internal transcribed spacers; juveniles; males; new geographic records; new species; restriction fragment length polymorphism; ribosomal RNA; soil nematodes; species differences; tail; vulva; Oregon
Abstract:
... A new species of cyst nematode, Globodera ellingtonae, is described from soil collected from a field in Oregon. Second-stage juveniles (J2) of the species are characterized by body length of 365-515 mm, stylet length of 19-22.5 mm, basal knobs rounded posteriorly and pointed anteriorly, tail 39-55 mm, hyaline tail terminus 20-32.5 mm, and tail tapering uniformly but abruptly narrowing and constric ...
Handle:
10113/54284
PubMed:
23483076
PubMed Central:
PMC3593265

14. Supplemental description of Paraphelenchus acontioides (Tylenchida: Aphelenchidae, Paraphelenchinae), with ribosomal DNA trees and a morphometric compendium of female Paraphelenchus

Author:
Skantar, Andrea M.,   et al.   ; Carta, Lynn K.; Handoo, Zafar A.; Baynes, Melissa A.;     Show all 4 Authors
Source:
Nematology 2011 v.13 no.8 pp. 887
ISSN:
1388-5545
Subject:
Aphelenchus avenae; Bromus tectorum; Fusarium; Hypocreaceae; Tylenchidae; ecology; females; fungi; grasses; oviducts; phylogeny; ribosomal DNA; sisters; stems; Colorado
Abstract:
... Nematodes were isolated from surface-sterilised stems of cheatgrass, Bromus tectorum (Poaceae), in Colorado, grown on Fusarium (Hypocreaceae) fungus culture, and identified as Paraphelenchus acontioides. Morphometrics and micrographic morphology of this species are given to supplement the original description and expand the comparative species diagnosis. A tabular morphometric compendium of the fe ...
Handle:
10113/54029
DOI:
10.1163/138855411X560968

15. Multiplex Real-Time PCR Assays for the Identification of the Potato Cyst and Tobacco Cyst Nematodes

Author:
Skantar, Andrea M.,   et al.   ; Nakhla, Mark K.; Owens, Kristina J.; Li, Wenbin; Wei, Gang; Levy, Laurene;     Show all 6 Authors
Source:
Plant disease 2010 v.94 no.8 pp. 959
ISSN:
0191-2917
Subject:
Globodera pallida; Globodera rostochiensis; Globodera tabacum; plant pests; cyst nematodes; pest identification; polymerase chain reaction; ribosomal DNA; internal transcribed spacers; rapid methods; quantitative analysis
Abstract:
... TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set specific for G. pallida (pale potato cyst nematode) multiplexed with another primer-probe set specific for G. rostochiensis (golden potato cyst n ...
Handle:
10113/44018
DOI:
10.1094/PDIS-94-8-0959

16. Molecular characterization and phylogenetic evaluation of the Hsp90 gene from selected nematodes

Author:
Skantar, Andrea M.,   et al.   ; Carta, Lynn K.;     Show all 2 Author
Source:
Journal of nematology 2004 v.36 no.4 pp. 466
ISSN:
0022-300X
Subject:
Nematoda; plant parasitic nematodes; free-living nematodes; heat shock proteins; complementary DNA; ribosomal DNA; nucleotide sequences; amino acid sequences; sequence homology; phylogeny
Abstract:
... While multiple genes are optimal for corroborating nematode phylogenies, only a few are commonly used. Here we examine the phylogenetic potential of the nuclear Hsp90 chaperone gene. We used degenerate primers to obtain partial Hsp90 sequences from several plant-parasitic and free-living nematodes. Hsp90 was single-copy in Heterodera glycines and Meloidogyne javanica, similar to the situation for ...
Handle:
10113/7242
PubMed:
19262827
PubMed Central:
PMC2620790

17. Amplification of Hsp90 Homologs from Plant-Parasitic Nematodes Using Degenerate Primers and Ramped Annealing PCR

Author:
Skantar, Andrea M.,   et al.   ; Carta, Lynn K.;     Show all 2 Author
Source:
Biotechniques 2000 v.29 no.6 pp. 1182-1184
ISSN:
0736-6205
Subject:
plant parasitic nematodes; Bursaphelenchus xylophilus; polymerase chain reaction; DNA primers; heat shock proteins
Handle:
10113/44273
PubMed:
11126117

18. Toxicity of 2,4-diacetylphloroglucinol (DAPG) to plant-parasitic and bacterial-feeding nematodes

Author:
Skantar, Andrea M.,   et al.   ; Meyer, Susan L.F.; Halbrendt, John M.; Carta, Lynn K.; Liu, Ting; Abdelnabby, Hazem M.E.; Vinyard, Bryan T.;     Show all 7 Authors
Source:
Journal of nematology 2009 v.41 no.4 pp. 274
Subject:
antibiotics; Pseudomonas fluorescens; beneficial microorganisms; plant parasitic nematodes; Heterodera glycines; Meloidogyne incognita; Pratylenchus scribneri; Xiphinema americanum; nontarget organisms; Caenorhabditis elegans
Abstract:
... The antibiotic 2,4-diacetylphloroglucinol (DAPG) is produced by some isolates of the beneficial bacterium Pseudomonas fluorescens. DAPG is toxic to many organisms, and crop yield increases have been reported after application of DAPG-producing P. fluorescens. This study was conducted to determine whether DAPG is toxic to selected nematodes. The plant-parasitic nematodes Heterodera glycines, Meloid ...
Handle:
10113/49736

19. Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P. convallariae and P. fallax (Nematoda: Pratylenchidae)

Author:
Skantar, Andrea M.,   et al.   ; Handoo, Zafar A.; Carta, Lynn K.;     Show all 3 Authors
Source:
Nematology 2001 v.3 no.pt.6 pp. 607
ISSN:
1388-5545
Subject:
Pratylenchus; new species; anatomy and morphology; body measurements; ribosomal DNA; nucleotide sequences; chemotaxonomy; plant parasitic nematodes; Poa pratensis; Festuca arundinacea; root lesion nematodes; Virginia
Abstract:
... Pratylenchus arlingtoni n. sp. from the rhizosphere of grasses Poa pratensis and Festuca arundinacea at Arlington National Cemetery, VA, USA is characterised by six to eight lines in the lateral field, and pyriform to slightly overlapping pharyngeal glands. Morphological comparisons are made with lesion nematodes having similar morphometrics, six lateral lines, or crenate tail tips. Molecular sequ ...
Handle:
10113/43158

20. Taxonomy, morphology and phylogenetics of coffee-associated root-lesion nematodes, Pratylenchus spp

Author:
Skantar, Andrea M.,   et al.   ; Handoo, Zafar A.; Carta, Lynn K.;     Show all 3 Authors
Subject:
Pratylenchus; plant parasitic nematodes; Coffea; animal taxonomy; animal morphology; phylogeny; taxonomic keys; scanning electron microscopy
Handle:
10113/34068