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1. Comparison of Swabbing Solution Volume and gDNA Extraction Kits on DNA Recovery from Rigid Surface

Author:
Ok, Yeon Jeong; You, Hee Sang; Lee, Song Hee; Sung, Ho Joong; Kang, Hee-Gyoo; Hyun, Sung Hee
Source:
Indian journal of microbiology 2020 v.60 no.2 pp. 206-213
ISSN:
0046-8991
Subject:
Staphylococcus aureus,   etc   ; DNA; Escherichia coli; bacteria; cotton; polysorbates;     Show all 6 Subjects
Abstract:
... For bacteria sampling studies, various collection methods have been used to identify bacteria. To obtain accurate information about bacteria, high quality samples should be obtained. In order to obtain a high quality sample, a stable and large number of DNA copies must be collected. This study compared the efficiency of different methods of bacterial gDNA extraction and bacteria collection accordi ...
DOI:
10.1007/s12088-019-00854-6
PubMed:
32255853
PubMed Central:
PMC7105554

2. The impact of two-component sensorial network in staphylococcal speciation

Author:
Haag, AF; Rapun-Araiz, Beatriz; Haag, Andreas F; Solano, Cristina; Lasa, Iñigo
Source:
Current opinion in microbiology 2020 v.55 pp. 40-47
ISSN:
1369-5274
Subject:
Staphylococcus aureus,   etc   ; Staphylococcus saprophyticus; bacteria; case studies; niches;     Show all 5 Subjects
Abstract:
... Bacteria use two-component systems (TCSs) to sense and respond to their environments. Free-living bacteria usually contain dozens of TCSs, each of them responsible for sensing and responding to a different range of signals. Differences in the content of two-component systems are related with the capacity of the bacteria to colonize different niches or improve the efficiency to grow under the condi ...
DOI:
10.1016/j.mib.2020.02.004

3. Structural insights into the conformational change of Staphylococcus aureus NreA at C-terminus

Author:
Sangare, Lancine; Chen, Wanbiao; Wang, Chengliang; Chen, Xiaobao; Wu, Minhao; Zhang, Xuan; Zang, Jianye
Source:
Biotechnology letters 2020 v.42 no.5 pp. 787-795
ISSN:
0141-5492
Subject:
Staphylococcus aureus,   etc   ; crystal structure; genes; histidine kinase; mutants; nitrates;     Show all 6 Subjects
Abstract:
... Staphylococcus aureus is an anaerobic facultative microorganism that features the NreABC system for nitrate respiration. NreB is the sensor histidine kinase that phosphorylates the response regulator NreC to stimulate the expression of target genes. NreA is a nitrate sensor which dissociates from NreB in the present of nitrate and relieves its inhibition on NreB. However, the molecular basis of ho ...
DOI:
10.1007/s10529-020-02807-2

4. Ultrasound-accelerated green synthesis of new quinolin-2-thione derivatives and antimicrobial evaluation against Escherichia coli and Staphylococcus aureus

Author:
Geesi, Mohammed H.; Moustapha, Moustapha E.; Bakht, Mohammed A.; Riadi, Yassine
Source:
Sustainable chemistry and pharmacy 2020 v.15 pp. 100195
ISSN:
2352-5541
Subject:
Staphylococcus aureus,   etc   ; Escherichia coli; antibacterial properties; green chemistry; ultrasonic treatment;     Show all 5 Subjects
Abstract:
... An efficient, green strategy to access to a new series of quinolinthiones was investigated. The targeted derivatives have been prepared from arylhydrazides and 2-thiocoumarin under ultrasound irradiation in refluxing water. The remarkable features of this strategy are simple procedure, high conversion, cleaner with short reaction times. All resultant compounds were screened against bacterial strai ...
DOI:
10.1016/j.scp.2019.100195

5. New sequential‐touch method to determine bacterial contact transfer rate from finger to surface

Author:
Zhao, P.; Li, Y.
Source:
Journal of applied microbiology 2019 v.127 no.2 pp. 605-615
ISSN:
1364-5072
Subject:
Staphylococcus aureus,   etc   ; bacteria; glass; models;     Show all 4 Subjects
Abstract:
... AIMS: Infection can occur via surface touch. A new method was developed to more accurately evaluate the bacterial transfer rate from a finger to a surface. METHODS AND RESULTS: Staphylococcus aureus was used as the model bacteria to inoculate a thumb. Sequential touches were made between the contaminated thumb and a series of clean microscope slides. The bacteria on the glass surface were cultured ...
DOI:
10.1111/jam.14332

6. Correction to: Phloretin reduces cell injury and inflammation mediated by Staphylococcus aureus via targeting sortase B and the molecular mechanism

Author:
Wang, Guizhen; Gao, Yawen; Wang, Hongsu; Wang, Jianfeng; Niu, Xiaodi
Source:
Applied microbiology and biotechnology 2019 v.103 no.14 pp. 5925-5927
ISSN:
0175-7598
Subject:
Staphylococcus aureus,   etc   ; inflammation; sortase B;     Show all 3 Subjects
Abstract:
... The images of cells under microscope in Figure 2 and Figure S2 were misused from Wang G et al. Front Cell Infect Microbiol. 2018 Nov 30;8:418. These images were generated in the same set of assays. ...
DOI:
10.1007/s00253-019-09924-7

7. Xylan-based hydrogels for potential skin care application

Author:
Fu, Gen-Que; Zhang, Sheng-Chun; Chen, Ge-Gu; Hao, Xiang; Bian, Jing; Peng, Feng
Source:
International journal of biological macromolecules 2020 v.158 pp. 244-250
ISSN:
0141-8130
Subject:
Staphylococcus aureus,   etc   ; Bacillus subtilis; antibacterial properties; biocompatible materials; crosslinking; gelatin; glycerol; hydrogels; nicotinamide; texture; xylan; yeasts;     Show all 12 Subjects
Abstract:
... Skin care biomaterials from natural compounds are increasingly needed in recent. We demonstrate a simple strategy to fabricate the dialdehyde xylan (DAX) crosslinked hydrogel with skin care potential. The hydrogel mainly consists of dialdehyde xylan, which is used as crosslinker for gelatin (G). Glycerol (Gly) and nicotinamide (NCA) are introduced here for improving the texture, antibacterial prop ...
DOI:
10.1016/j.ijbiomac.2020.04.235

8. Continuous production of antibacterial carboxymethyl chitosan-zinc supramolecular hydrogel fiber using a double-syringe injection device

Author:
Wang, Yu-Long; Zhou, Ya-Ning; Li, Xin-Yu; Huang, Ju; Wahid, Fazli; Zhong, Cheng; Chu, Li-Qiang
Source:
International journal of biological macromolecules 2020 v.156 pp. 252-261
ISSN:
0141-8130
Subject:
Staphylococcus aureus,   etc   ; Escherichia coli; antibacterial properties; fluorescence; hydrogels; porous media; scanning electron microscopes; zinc;     Show all 8 Subjects
Abstract:
... Large-scale production of an antibacterial hydrogel is of critical importance for its practical application in biomedical field. In this regard, herein we report on the construction of a double-syringe injection device by using all the commercial parts and its use for continuous production of carboxymethyl chitosan-zinc (CMCh-Zn) supramolecular hydrogel fiber. The resultant CMCh-Zn hydrogel fibers ...
DOI:
10.1016/j.ijbiomac.2020.04.073

9. Continuous, rapid concentration of foodborne bacteria (Staphylococcus aureus, Salmonella typhimurium, and Listeria monocytogenes) using magnetophoresis-based microfluidic device

Author:
Jung, Taekeon; Jung, Yugyung; Ahn, Jaehyeon; Yang, Sung
Source:
Food control 2020 v.114 pp. 107229
ISSN:
0956-7135
Subject:
Staphylococcus aureus,   etc   ; Listeria monocytogenes; Salmonella Typhimurium; bacteria; biosensors; cabbage; magnetic materials; milk; polyethylene;     Show all 9 Subjects
Abstract:
... Most biosensors use small amounts (a few tens of microliters) of a sample to detect bacteria. However, it is necessary to detect bacteria with at least a few milliliters of the sample to obtain statistically significant results. Therefore, the bacteria concentration process is critical for the rapid and accurate detection of bacteria. In addition, most bacteria concentration devices have a low thr ...
DOI:
10.1016/j.foodcont.2020.107229

10. Effect of N-terminal modification on the antimicrobial activity of nisin

Author:
Yu, Xuejian; Lu, Naiyan; Wang, Jiyue; Chen, Zhe; Chen, Chen; Regenstein, Joe Mac; Zhou, Peng
Source:
Food control 2020 v.114 pp. 107227
ISSN:
0956-7135
Subject:
Staphylococcus aureus,   etc   ; GRAS substances; World Health Organization; antimicrobial peptides; antimicrobial properties; bacteria; chitosan; crosslinking; lipids; nisin;     Show all 10 Subjects
Abstract:
... Nisin, the only antimicrobial peptide that has GRAS (generally considered as safe) status from both WHO and FDA, has the potential to become an important natural antibiotic as bacteria become more resistant to traditional antibiotics. Many studies have focused on how modification of nisin affects antimicrobial activity. In this study, nisin and chitosan were crosslinked using genipin to modify the ...
DOI:
10.1016/j.foodcont.2020.107227

11. Impact of non-thermal dielectric barrier discharge plasma on Staphylococcus aureus and Bacillus cereus and quality of dried blackmouth angler (Lophiomus setigerus)

Author:
Choi, Man-Seok; Jeon, Eun Bi; Kim, Ji Yoon; Choi, Eun Ha; Lim, Jun Sup; Choi, Jinsung; Park, Shin Young
Source:
Journal of food engineering 2020 v.278 pp. 109952
ISSN:
0260-8774
Subject:
Staphylococcus aureus,   etc   ; Bacillus cereus; color; fillets; fisheries; flavor; food production; inoculum; meat; pH; texture; thiobarbituric acid;     Show all 12 Subjects
Abstract:
... This study investigated the effects of dielectric barrier discharge (DBD) plasma against Staphylococcus aureus and Bacillus cereus (initial inoculum of 8–9 log₁₀ CFU/mL) on the surface of dried blackmouth angler, and the physicochemical [pH, Hunter colors, thiobarbituric acid reactive (TBA)] and sensorial quality on the dried blackmouth angler. Reductions in S. aureus and B. cereus counts on the f ...
DOI:
10.1016/j.jfoodeng.2020.109952

12. Versatile approach to densely substituted isoxazolines and pyrazolines: Focus on a quaternary carbon center as a constitutive feature

Author:
Abdelli, Abderrahmen; Gharsa, Haythem; Jmaï, Momtez; Gaucher, Anne; Efrit, Mohamed Lotfi; M'rabet, Hedi; Prim, Damien
Source:
Tetrahedron letters 2020 v.61 no.23 pp. 151958
ISSN:
0040-4039
Subject:
Staphylococcus aureus,   etc   ; antibacterial properties; carbon; chemical structure; cycloaddition reactions; heterocyclic compounds; minimum inhibitory concentration;     Show all 7 Subjects
Abstract:
... A new family of isoxazolines has been obtained via 1,3-dipolar cycloaddition in good to high yields under mild conditions. Our approach focused on construction of the heterocyclic ring and direct access to a quaternary carbon center at position 5. The methodology has also been extended to pyrazoline analogues. The antibacterial activity of these compounds was evaluated, showing specific antibacter ...
DOI:
10.1016/j.tetlet.2020.151958

13. Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing

Author:
Zhang, Chengwei; Wang, Feipeng; Zhao, Si; Kang, Guiting; Song, Jinling; Li, Lu; Yang, Jinxiao
Source:
The crop journal 2020 v.8 no.3 pp. 418-423
ISSN:
2214-5141
Subject:
Staphylococcus aureus,   etc   ; Streptococcus pyogenes; breeding; cytosine; gene editing; mutation; rice; uncertainty;     Show all 8 Subjects
Abstract:
... Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus (SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif (PAM) that the canonical Streptococcus ...
DOI:
10.1016/j.cj.2020.03.002

14. Studies toward the enantioselective synthesis of neurymenolide A: Construction of the macrocyclic core via Claisen rearrangement

Author:
Masuda, Masahiro; Sakurai, Natsuki; Ogura, Yusuke; Murase, Tetsuji; Kawasaki, Tsuneomi; Aiba, Shohei; Mori, Naoki; Watanabe, Hidenori; Takikawa, Hirosato
Source:
Tetrahedron letters 2020 pp. 151825
ISSN:
0040-4039
Subject:
Staphylococcus aureus,   etc   ; Enterococcus faecium; Rhodophyta; chemical reactions; chemical structure; enantioselective synthesis; enantioselectivity; macrolides;     Show all 8 Subjects
Abstract:
... Neurymenolides, α-pyrone macrolides isolated from the Fijian red alga Neurymenia fraxinifolia, are anti-methicillin-resistant Staphylococcus aureus and anti-vancomycin-resistant Enterococcus faecium compounds. In this study, the macrocyclic core of neurymenolide A was constructed in an enantioselective manner by employing Claisen rearrangement as the key step. ...
DOI:
10.1016/j.tetlet.2020.151825

15. Detection of bacteria in bovine ovarian follicular fluid

Author:
Salary, A.; Kafi, M.; Derakhshandeh, A.; Moezzi, M.S.
Source:
Letters in applied microbiology 2020 v.70 no.3 pp. 137-142
ISSN:
0266-8254
Subject:
Staphylococcus aureus,   etc   ; Escherichia coli; anaerobes; bacteria; dairy cows; follicular fluid; lipopolysaccharides; pulsed-field gel electrophoresis; uterus;     Show all 9 Subjects
Abstract:
... The aim of this study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. In all, 17 samples of paired follicular fluids and uterine contents collected from post‐slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Furthermore, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also in ...
DOI:
10.1111/lam.13254

16. Treatment of pastirma with pulsed UV light: Modeling of Staphylococcus aureus inactivation and assessment of quality changes

Author:
Keklik, Nene Meltem
Source:
Food science and technology international 2020 v.26 no.2 pp. 185-198
ISSN:
1532-1738
Subject:
Staphylococcus aureus,   etc   ; Weibull statistics; color; mathematical models; quartz; thiobarbituric acid-reactive substances; ultraviolet radiation;     Show all 7 Subjects
Abstract:
... The efficacy of pulsed UV (PUV) light treatment carried out in a wide range of fluence was investigated on pastirma slices by characterizing Staphylococcus aureus inactivation using mathematical models and by assessing the treatment effects on quality attributes. Pastirma slices inoculated on top surface with S. aureus were subjected to pulsed UV light for 5, 15, 25, 35, and 45 s at 5, 8, and 13 c ...
DOI:
10.1177/1082013219889231

17. Uranium bioremediation by acid phosphatase activity of Staphylococcus aureus biofilms: Can a foe turn a friend?

Author:
Shukla, Sudhir K.; Hariharan, S.; Rao, T. Subba
Source:
Journal of hazardous materials 2020 v.384 pp. 121316
ISSN:
0304-3894
Subject:
Staphylococcus aureus,   etc   ; acid phosphatase; biofilm; biomass; bioremediation; enzyme activity; nitrates; phenotype; phosphates; plankton; uranium; uranyl ions;     Show all 12 Subjects
Abstract:
... In this study, Staphylococcus aureus biofilms, which are considered a foe for being pathogenic, were tested for their uranium bioremediation capacity to find out if they can turn out to be a friend. Acid phosphatase activity, which is speculated to aid in bio-precipitation of U(VI) from uranyl nitrate solution, was assayed in biofilms of seven different S. aureus strains. The presence of acid phos ...
DOI:
10.1016/j.jhazmat.2019.121316

18. Chromobacterium violaceum delivers violacein, a hydrophobic antibiotic, to other microbes in membrane vesicles

Author:
Choi, Seong Yeol; Lim, Sungbin; Cho, Gayoung; Kwon, Jisoo; Mun, Wonsik; Im, Hansol; Mitchell, Robert J.
Source:
Environmental microbiology 2020 v.22 no.2 pp. 705-713
ISSN:
1462-2912
Subject:
Staphylococcus aureus,   etc   ; Chromobacterium violaceum; antibacterial properties; antibiotics; hydrophobicity; microorganisms; mutants; solubility; viability; violacein;     Show all 10 Subjects
Abstract:
... This study describes Chromobacterium violaceum's use of extracellular membrane vesicles (MVs) to both solubilize and transport violacein to other microorganisms. Violacein is a hydrophobic bisindole with known antibiotic activities against other microorganisms. Characterization of the MVs found they carried more violacein than protein (1.37 ± 0.19‐fold), suggesting they may act as a reservoir for ...
DOI:
10.1111/1462-2920.14888

19. Production of characteristic volatile markers and their relation to Staphylococcus aureus growth status in pork

Author:
Hu, K.L.; Yu, X.Q.; Chen, J.; Tang, J.N.; Wang, L.Z.; Li, Y.M.; Tang, C.
Source:
Meat science 2020 v.160 pp. 107956
ISSN:
0309-1740
Subject:
Staphylococcus aureus,   etc   ; biomarkers; cost effectiveness; livestock and meat industry; minced pork; mixed culture; raw meat;     Show all 7 Subjects
Abstract:
... The 3-methyl-butanal and 3-methyl-butanoic acid are known as fingerprint compounds from Staphylococcus aureus. In this study, we investigated production of these two volatile biomarkers and their correlation to S. aureus growth in pork. Both 3-methyl-butanal and 3-methyl-butanoic acid presented high specificity for S. aureus in either media or pork. In sterile minced pork and pork broth, productio ...
DOI:
10.1016/j.meatsci.2019.107956

20. Response surface methodology can be used to predict photoinactivation of foodborne pathogens using Rose Bengal excited by 530 nm LED

Author:
dos Santos, Adriele R.; da Silva, Alex F.; de Freitas, Camila F.; da Silva, Marcos V.; Bona, Evandro; Nakamura, Celso V.; Hioka, Noboru; Mikcha, Jane M. G.
Source:
Journal of food safety 2020 v.40 no.1 pp. e12736
ISSN:
0149-6085
Subject:
Staphylococcus aureus,   etc   ; Salmonella Typhimurium; antibacterial properties; empirical models; food pathogens; lighting; prediction; response surface methodology;     Show all 8 Subjects
Abstract:
... In this work, the photodynamic bactericidal effect of Rose Bengal (RB), combined with green LED light, against Staphylococcus aureus and Salmonella Typhimurium, was investigated. The interaction of RB concentration and illumination time was evaluated using a response surface methodology, and a second‐order polynomial empirical model was adjusted to the experimental data. The derived model predicte ...
DOI:
10.1111/jfs.12736