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1. Anatomic site of application of ice-nucleating active bacteria affects supercooling in the Colorado potato beetle (Coleoptera: Chrysomelidae)

Author:
Steigerwald, K.A.,   et al.   ; Lee, R.E. Jr.; Wyman, J.A.; Costanzo, J.P.; Lee, M.R.;     Show all 5 Authors
Source:
Environmental entomology 1996 v.25 no.2 pp. 465-469
ISSN:
0046-225X
Subject:
Leptinotarsa decemlineata; Pseudomonas syringae; dose response; topical application; cold tolerance; supercooling point; ice nucleation
Abstract:
... Most overwintering insects do not survive internal freezing and must avoid low temperatures or enhance the capacity of their body fluids to supercool to survive low temperature exposure. Recent reports have demonstrated that the application of ice-nucleating active microorganisms markedly diminishes supercooling. Topical application of as little as 20 ppm of a suspension of a freeze-dried preparat ...
DOI:
10.1093/ee/25.2.465