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- Sumitomo, Nobuyuki, et al. Show all 7 Authors
- European journal of biochemistry 2000 v.267 no.5 pp. 1510-1515
- Azospirillum irakense; Bacillus (bacteria); amino acid sequences; amino acids; calcium; culture media; enzyme activity; genes; ions; microorganisms; molecular weight; open reading frames; pH; pectate lyase; polyacrylamide gel electrophoresis; sequence homology; sodium hydroxide
- ... A pectate lyase (pectate transeliminase; ECâ22.214.171.124), designated Pelâ15E, was purified to homogeneity from a culture broth of alkaliphilic Bacillus sp. strain KSMâP15. The purified enzyme had a molecular mass of ââ33âkDa, as determined by SDS/PAGE, and a pI of ââpHâ9.2. Pelâ15E exhibited optimum activity at pHâ10.5 and 50â55âÂ°C in glycine/NaOH buffer. Pelâ15E had an ab ...