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1. Dietary Ginkgo biloba leaf extracts supplementation improved immunity and intestinal morphology, antioxidant ability and tight junction proteins mRNA expression of hybrid groupers (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) fed high lipid diets

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Ye, Chaoxia;     Show all 3 Authors
Source:
Fish & shellfish immunology 2019
ISSN:
1050-4648
Subject:
Epinephelus fuscoguttatus; Epinephelus lanceolatus; Ginkgo biloba; Oriental traditional medicine; antioxidant activity; antioxidants; apoptosis; aquaculture; caspase-3; caspase-8; caspase-9; catalase; complement; dietary supplements; feed additives; females; gene expression; glutathione peroxidase; glutathione-disulfide reductase; grouper; hepatoprotective effect; high fat diet; hybrids; immune response; immunoglobulin M; interleukin-10; intestines; leaf extracts; males; medicinal plants; messenger RNA; occludins; rapamycin; tight junctions; transforming growth factor beta 1
Abstract:
... For many years, Ginkgo biloba has been used as a traditional Chinese medicine because of its antioxidant, anti-inflammatory and hepatoprotective effects. The present study aimed to investigate the effects of dietary Ginkgo biloba leaf extract (GBLE) supplementation on immune response, intestinal morphology, antioxidant ability and tight junction proteins mRNA expression of hybrid groupers fed high ...
DOI:
10.1016/j.fsi.2019.09.034

2. Dietary Lycium barbarum extract administration improved growth, meat quality and lipid metabolism in hybrid grouper (Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀) fed high lipid diets

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Ye, Chaoxia;     Show all 3 Authors
Source:
Aquaculture 2019 v.504 pp. 190-198
ISSN:
0044-8486
Subject:
Epinephelus fuscoguttatus; Epinephelus lanceolatus; Lycium barbarum; abdominal fat; amino acids; dietary supplements; fatty acid composition; fatty acids; genes; grouper; high fat diet; hybrids; lipid content; lipid metabolism; low density lipoprotein cholesterol; meat composition; meat quality; muscles; protein deposition; triacylglycerols
Abstract:
... Growth, plasma biochemical parameters, meat quality, and expressions of lipid metabolism related genes were investigated in hybrid grouper fed high lipid diets supplemented with five levels (0, 0.5, 1, 2 and 10 g kg−1) of Lycium barbarum extract (LBE) for 8 weeks. Results showed that dietary LBE significantly improved growth and protein deposition, but reduced hepatic lipid content and abdominal f ...
DOI:
10.1016/j.aquaculture.2019.01.044

3. A simple and effective strategy based on sodium gallate-exfoliated graphene for the simultaneous voltammetric determination of guaiacol and vanillin

Author:
Tan, Xiaohong,   et al.   ; Li, Chenchen; Fu, Ju; Song, Xinjian; Li, Qunfang;     Show all 5 Authors
Source:
Analytical methods 2019 v.11 no.32 pp. 4099-4105
ISSN:
1759-9679
Subject:
analytical methods; detection limit; dimethylformamide; electrochemistry; graphene; guaiacol; nanosheets; oxidation; sodium; ultrasonic treatment; vanillin
Abstract:
... High concentration graphene nanosheets were easily prepared through simple liquid-phase exfoliation of graphite powder in N,N-dimethylformamide with the assistance of sodium gallate (SG). The concentration of the obtained graphene dispersion reached 2.0 mg mL⁻¹ only after three hours of ultrasonic treatment. Sodium gallate-exfoliated graphene nanosheets (SG-GNs) showed remarkable signal enhancemen ...
DOI:
10.1039/c9ay01018a

4. The effects of dietary Lycium barbarum extract on growth performance, liver health and immune related genes expression in hybrid grouper (Epinephelus lanceolatus♂ × E. fuscoguttatus♀) fed high lipid diets

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Ye, Chaoxia; Lin, Heizhao;     Show all 4 Authors
Source:
Fish & shellfish immunology 2019 v.87 pp. 847-852
ISSN:
1050-4648
Subject:
Epinephelus; Lycium barbarum; antioxidant activity; antioxidant enzymes; antioxidant genes; apoptosis; caspase-3; caspase-8; caspase-9; dietary supplements; feed conversion; females; fish feeding; grouper; growth performance; high fat diet; hybrids; inflammation; interleukin-10; interleukin-8; liver; males; messenger RNA; necrosis; plant extracts; specific growth rate; transforming growth factor beta 1; weight gain
Abstract:
... Growth performance, hepatic morphology and antioxidant ability, and expressions of antioxidant, inflammatory and apoptosis related genes were investigated in hybrid grouper fed high lipid diets containing 0, 0.5, 1, 2 and 10 g kg−1Lycium barbarum extract (LBE) for 8-week feeding. The study showed that dietary LBE significantly increased weight gain rate (WGR) and specific growth rate (SGR) of fish ...
DOI:
10.1016/j.fsi.2019.02.016

5. Liver transcriptome analysis and de novo annotation of the orange-spotted groupers (Epinephelus coioides) under cold stress

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Xu, Minglei; Liu, Qingying; Ye, Huaqun; Zou, Cuiyun; Ye, Chaoxia;     Show all 7 Authors
Source:
Comparative biochemistry and physiology 2019 v.29 pp. 264-273
ISSN:
1744-117X
Subject:
Epinephelus coioides; Staphylococcus aureus; Vibrio cholerae; cDNA libraries; cell adhesion molecules; cold; cold stress; complementary DNA; fatty acids; gene expression regulation; grouper; immunosuppression; liver; peroxisome proliferator-activated receptors; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; temperature; transcriptomics; unigenes; China
Abstract:
... Cold stress has caused great economic loss in fish culture worldwide. Orange-spotted grouper (Epinephelus coioides) is one of the most serious lost aquatic animals in 2016 cold fronts in South China. However, the molecular mechanism of grouper's cold resistance has remained largely unknown. In the present study, HiSeq™2000 (Illumina) was used to analyze the transcriptomic profiles of the liver fro ...
DOI:
10.1016/j.cbd.2018.12.008

6. Extract of Ginkgo biloba leaves mediated biosynthesis of catalytically active and recyclable silver nanoparticles

Author:
Tan, Xiaohong,   et al.   ; Wang, Fengping; Zhang, Wanming; Wang, Zhibao; Li, Yaxin; Li, Wei;     Show all 6 Authors
Source:
Colloids and surfaces 2019 v.563 pp. 31-36
ISSN:
0927-7757
Subject:
Ginkgo biloba; biosynthesis; catalytic activity; colloids; leaves; methyl orange; nanosilver; p-nitrophenol; pollutants; rhodamines; synthesis
Abstract:
... In the present study, catalytically active Ag nanoparticles were biosynthesized by the extract of Ginkgo biloba leaves through a facile and green manner. Well-dispersed Ag nanoparticles in spherical shape with size of 20–40 nm were obtained. Catalyzing the reduction of four typical pollutants, 4-nitrophenol, congo red, methyl orange, and rhodamine B, were achieved using the synthesized Ag nanopart ...
DOI:
10.1016/j.colsurfa.2018.11.054

7. Physiological, immune responses and liver lipid metabolism of orange-spotted grouper (Epinephelus coioides) under cold stress

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Liu, Qingying; Ye, Huaqun; Zou, Cuiyun; Xu, Minglei; Zhang, Yufan; Ye, Chaoxia;     Show all 8 Authors
Source:
Aquaculture 2019 v.498 pp. 545-555
ISSN:
0044-8486
Subject:
DNA damage; Epinephelus coioides; alanine transaminase; apoptosis; aspartate transaminase; blood; cold stress; environmental factors; financial economics; fish culture; gene expression; gene expression regulation; genes; grouper; immune response; interleukin-1alpha; lipid metabolism; liver; messenger RNA; water temperature
Abstract:
... Low temperature is an important environmental factor in aquaculture. Cold stress has caused great economic loss in fish culture worldwide. In the present study, we investigated the effects of low temperature on biochemical parameters, DNA damage, immune and lipid metabolism related genes expression in orange spotted grouper. In the experiment, water temperature decreased from 28 °C to 13 °C at a r ...
DOI:
10.1016/j.aquaculture.2018.08.051

8. Facilitating Subtiligase-Catalyzed Peptide Ligation Reactions by Using Peptide Thioester Substrates

Author:
Tan, Xiaohong,   et al.   ; Yang, Renliang; Liu, Chuan-Fa;     Show all 3 Authors
Source:
Organic letters 2018 v.20 no.21 pp. 6691-6694
ISSN:
1523-7052
Subject:
chemical reactions; chemical structure; enzymatic treatment; synthesis; thioesters
Abstract:
... By facilitating formation of the acyl–enzyme intermediate, peptide thioesters can largely promote subtiligase-catalyzed ligation reactions over their ester counterparts. This offers a significant addition to the repertoire of enzymatic methods for protein chemical synthesis and modification. ...
DOI:
10.1021/acs.orglett.8b02747

9. Effects of replacing fish meal with rendered animal protein blend on growth performance, hepatic steatosis and immune status in hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

Author:
Tan, Xiaohong,   et al.   ; Ye, Huaqun; Zhou, Yuanyuan; Su, Ningning; Wang, Anli; Sun, Zhenzhu; Zou, Cuiyun; Liu, Qingying; Ye, Chaoxia;     Show all 9 Authors
Source:
Aquaculture 2019 pp. 734203
ISSN:
0044-8486
Subject:
Epinephelus; animal proteins; apolipoprotein A-I; blood meal; body weight; carnitine palmitoyltransferase; caspase-3; caspase-8; caspase-9; droplets; fatty liver; fatty-acid synthase; feed conversion; fish meal; gene expression regulation; genes; grouper; growth performance; high density lipoprotein cholesterol; histology; hybrids; interleukin-10; interleukin-8; juveniles; lipid content; lipoprotein lipase; liver; low density lipoprotein cholesterol; messenger RNA; peroxisome proliferator-activated receptor alpha; poultry meal; protein efficiency ratio; protein sources; shrimp; spray drying; transforming growth factor beta 1; vacuoles; weight gain
Abstract:
... An 8-week growth trial was conducted to evaluate the effects of substitution of fish meal (FM) by rendered animal protein blend (APB) consisting of poultry by-product meal (PBM), shrimp meal (SM) and spray-dried blood meal (BM) in diets of juvenile hybrid grouper, Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂ (32.51 ± 0.31 g). A basal diet (FM70) with FM as the sole protein source was comp ...
DOI:
10.1016/j.aquaculture.2019.734203

10. Effects of dietary plant protein sources influencing hepatic lipid metabolism and hepatocyte apoptosis in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Author:
Tan, Xiaohong,   et al.   ; Ye, Huaqun; Xu, Minglei; Chen, Leling; Chen, Shu; Zou, Cuiyun; Sun, Zhenzhu; Liu, Qingying; Ye, Chaoxia; Wang, Anli;     Show all 10 Authors
Source:
Aquaculture 2019 v.506 pp. 437-444
ISSN:
0044-8486
Subject:
Epinephelus; apolipoprotein A-I; apoptosis; carnitine palmitoyltransferase; carnivores; caspase-3; caspase-7; caspase-8; corn gluten meal; cottonseed meal; droplets; feed conversion; fish meal; gene expression regulation; genes; grouper; growth performance; hepatosomatic index; high density lipoprotein cholesterol; histology; hybrids; juveniles; lipid content; lipid metabolism; lipoprotein lipase; liver; low density lipoprotein cholesterol; messenger RNA; peroxisome proliferator-activated receptor alpha; plant proteins; protein sources; soybean meal
Abstract:
... A 56-day feeding trial was conducted to evaluate the effects of replacing dietary fish meal (FM) with a complementary mixture of plant protein (PP) consisting of soybean meal (SBM), corn gluten meal (CGM) and cottonseed meal (CSM) on hepatic lipid metabolism and hepatocyte apoptosis in juvenile hybrid grouper, Epinephelus lanceolatus♂ × E. fuscoguttatus♀ (31.64 ± 0.82 g). A basal diet (FM60) with ...
DOI:
10.1016/j.aquaculture.2019.03.075

11. Dietary supplementation with taurine improves ability to resist ammonia stress in hybrid snakehead (Channa maculatus♀ × Channa argus♂)

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Zhu, Xuan; Ye, Chaoxia;     Show all 4 Authors
Source:
Aquaculture research 2018 v.49 no.10 pp. 3400-3410
ISSN:
1355-557X
Subject:
Channa; alanine transaminase; alkaline phosphatase; ammonia; ammonium nitrogen; apoptosis; aspartate transaminase; catalase; dietary supplements; fish; gene expression; genes; glutathione-disulfide reductase; heat-shock protein 70; juveniles; messenger RNA; respiratory burst; survival rate; tanks; taurine; triacylglycerols; urea nitrogen
Abstract:
... This study determined the effect of dietary supplementation with taurine on plasma biochemical indices, blood cell apoptosis rate, survival rate and expression of catalase (CAT), glutathione reductase (GR) and heat‐shock protein 70 (HSP70) gene in juvenile hybrid snakehead under ammonia stress. Six diets were formulated to contain 0, 3.0, 6.0, 9.0, 12.0 and 15.0 g/kg taurine. Each diet was fed to ...
DOI:
10.1111/are.13804

12. Effects of dietary dandelion extracts on growth performance, liver histology, immune-related gene expression and CCl4 resistance of hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Xu, Minglei; Liu, Qingying; Ye, Huaqun; Zou, Cuiyun; Zhou, Yuanyuan; Su, Ningning; Chen, Leling; Wang, Anli; Ye, Chaoxia;     Show all 11 Authors
Source:
Fish & shellfish immunology 2019 v.88 pp. 126-134
ISSN:
1050-4648
Subject:
Epinephelus; Taraxacum; antioxidant enzymes; antioxidant genes; blood serum; body composition; catalase; chemokine CCL4; crude protein; dietary supplements; farmed fish; feed additives; feed conversion; females; gene expression; glutathione peroxidase; glutathione-disulfide reductase; grouper; growth performance; hepatocytes; histology; hybrids; kidneys; lipids; liver; liver function; males; messenger RNA; muscles; plant extracts; spleen; survival rate
Abstract:
... The study investigated the effects of dietary supplementation with dandelion extracts (DE) on growth performance, feed utilization, body composition, serum biochemical, liver histology, immune-related gene expression and CCl4 resistance of hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). A basal diet supplemented with DE at 0% (diet 0%), 0.1% (diet 0.1%), 0.2% (diet 0.2%), 0 ...
DOI:
10.1016/j.fsi.2019.02.031

13. Effects of dietary Panax notoginseng extract on growth performance, fish composition, immune responses, intestinal histology and immune related genes expression of hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) fed high lipid diets

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Ye, Huaqun; Zou, Cuiyun; Ye, Chaoxia; Wang, Anli;     Show all 6 Authors
Source:
Fish & shellfish immunology 2018 v.73 pp. 234-244
ISSN:
1050-4648
Subject:
Epinephelus fuscoguttatus; Epinephelus lanceolatus; Panax notoginseng; Toll-like receptor 3; alkaline phosphatase; antioxidant activity; antioxidant genes; antioxidants; blood; complement; crude protein; dietary supplements; feed conversion; foregut; glucose; grouper; growth performance; high fat diet; hindgut; histology; hybrids; immune response; immunoglobulins; interleukin-10; intestines; kidneys; low density lipoprotein cholesterol; midgut; muscles; protein efficiency ratio; superoxide dismutase; transforming growth factor beta 1; triacylglycerols; villi
Abstract:
... Growth, plasma biochemical parameters, fish composition, immune parameters, intestinal histology, and expressions of immune-related genes were examined in hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) that fed respectively six diets containing Panax notoginseng extract (PNE) at 0, 0.5, 1, 2, 4, and 10 g kg−1 after 8 weeks. Results indicated that dietary PNE significantly ...
DOI:
10.1016/j.fsi.2017.11.007

14. Effects of dietary ginkgo biloba leaf extract on growth performance, plasma biochemical parameters, fish composition, immune responses, liver histology, and immune and apoptosis-related genes expression of hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) fed high lipid diets

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Liu, Qingying; Ye, Huaqun; Zou, Cuiyun; Ye, Chaoxia; Wang, Anli; Lin, Heizhao;     Show all 8 Authors
Source:
Fish & shellfish immunology 2018 v.72 pp. 399-409
ISSN:
1050-4648
Subject:
Epinephelus; Ginkgo biloba; Oriental traditional medicine; Toll-like receptor 3; antioxidant genes; antioxidants; caspase-3; caspase-8; caspase-9; catalase; condition factor; crude protein; dietary supplements; feed conversion; food intake; grouper; growth performance; hepatosomatic index; high density lipoprotein; high fat diet; histology; hybrids; immune response; interleukin-10; kidneys; leaf extracts; leaves; lipemic effect; liver; liver function; low density lipoprotein; spleen; superoxide dismutase; transforming growth factor beta 1; trees
Abstract:
... For thousands of years, leaves from the Ginkgo biloba tree have been a common treatment in Chinese medicine. The present study was conducted to investigate the effects of dietary ginkgo biloba leaf extract (GBE) supplementation on growth performance, plasma biochemical parameters, fish composition, immune responses, liver histology, and immune and apoptosis-related genes expression of hybrid group ...
DOI:
10.1016/j.fsi.2017.10.022

15. The hepatoprotective effects of Radix Bupleuri extracts against D-galactosamine/lipopolysaccharide induced liver injury in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Author:
Tan, Xiaohong,   et al.   ; Zou, Cuiyun; Ye, Huaqun; Sun, Zhenzhu; Chen, Shu; Liu, Qingying; Xu, Minglei; Ye, Chaoxia; Wang, Anli;     Show all 9 Authors
Source:
Fish & shellfish immunology 2018 v.83 pp. 8-17
ISSN:
1050-4648
Subject:
Epinephelus; Toll-like receptor 3; animal models; antioxidant enzymes; antioxidant genes; apoptosis; caspase-3; caspase-9; cell viability; culture media; diet; enzyme activity; females; gene expression; gene expression regulation; grouper; hepatocytes; hepatoprotective effect; hybrids; inflammation; interleukin-1beta; interleukin-6; lipopolysaccharides; liver; males; medicinal plants; messenger RNA; plant extracts; poisoning; tumor necrosis factor-alpha
Abstract:
... The present study is aiming at evaluating the hepatoprotective of Radix Bupleuri extracts (RBE) on the d-galactosamine/lipopolysaccharide (D-GalN/LPS) induced liver injury of hybrid grouper in vitro and in vivo. In vitro, RBE (0, 200, 400 and 800 μg/ml) was added to the hybrid grouper primary hepatocytes before (pretreatment) the incubation of the hepatocytes with D-GalN (20 mM) plus LPS (1 μg/ml) ...
DOI:
10.1016/j.fsi.2018.08.047

16. Effects of dietary dandelion extract on intestinal morphology, antioxidant status, immune function and physical barrier function of juvenile golden pompano Trachinotus ovatus

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Zhou, Chuanpeng; Huang, Zhong; Tan, Lianjie; Xun, Pengwei; Huang, Qianqian; Lin, Heizhao; Ye, Chaoxia; Wang, Anli;     Show all 10 Authors
Source:
Fish & shellfish immunology 2018 v.73 pp. 197-206
ISSN:
1050-4648
Subject:
Taraxacum; Trachinotus ovatus; absorption; antioxidant activity; antioxidants; catalase; diet; digestion; fish; foregut; gene expression; gene expression regulation; genes; goblet cells; hindgut; immune response; intestines; juveniles; messenger RNA; midgut; muscles; occludins; shellfish; superoxide dismutase; tight junctions; villi
Abstract:
... Intestinal morphology, antioxidant status, immune function and tight junction proteins mRNA expression were examined in golden pompano (Trachinotus ovatus) that fed respectively six diets containing dandelion extracts (DE) at 0, 0.5, 1, 2, 4 and 10 g kg−1 after 8 weeks feeding. The study indicated that dietary DE significantly improved intestinal antioxidant abilities by increasing SOD, CAT, T-AOC ...
DOI:
10.1016/j.fsi.2017.12.020

17. Effects of dietary hawthorn extract on growth performance, immune responses, growth- and immune-related genes expression of juvenile golden pompano (Trachinotus ovatus) and its susceptibility to Vibrio harveyi infection

Author:
Tan, Xiaohong,   et al.   ; Sun, Zhenzhu; Huang, Zhong; Zhou, Chuanpeng; Lin, Heizhao; Tan, Lianjie; Xun, Pengwei; Huang, Qian;     Show all 8 Authors
Source:
Fish & shellfish immunology 2017 v.70 pp. 656-664
ISSN:
1050-4648
Subject:
Crataegus; Trachinotus ovatus; Vibrio harveyi; antioxidant activity; antioxidants; body weight; complement; diet; feed conversion; genes; growth performance; heat shock proteins; immune response; immunoglobulin M; insulin-like growth factor I; insulin-like growth factor II; interleukin-10; interleukin-8; juveniles; liver; messenger RNA; pathogens; protein efficiency ratio; specific growth rate; superoxide dismutase; transforming growth factor beta 1; tumor necrosis factor-alpha; weight gain
Abstract:
... The present study was conducted to investigate the effects of dietary hawthorn extract (HTE) supplementation on growth performance, immune responses, hepatic antioxidant abilities, growth- and immune-related and heat shock protein genes expression and resistance to the pathogen Vibrio harveyi in Trachinotus ovatus. A basal diet supplemented with HTE at 0 (Diet 1), 0.50 (Diet 2), 1.00 (Diet 3), 2.0 ...
DOI:
10.1016/j.fsi.2017.09.041

18. A novel metal-free two-dimensional material for photocatalytic water splitting – phosphorus nitride (γ-PN)

Author:
Tan, Xiaohong,   et al.   ; Ji, Yujin; Dong, Huilong; Liu, Meiyi; Hou, Tingjun; Li, Youyong;     Show all 6 Authors
Source:
RSC advances 2017 v.7 no.79 pp. 50239-50245
ISSN:
2046-2069
Subject:
aqueous solutions; density functional theory; hydrogen; molecular dynamics; nitrides; oxygen; phosphorus; photocatalysis; photocatalysts; semiconductors; spectral analysis; tensile strength
Abstract:
... Visible-light-driven water-splitting technology has attracted wide attention because of its sustainable and renewable production of hydrogen. The key to photocatalysis field is to search materials with suitable oxidizing and reducing potential. Herein we investigated the novel 2D γ-phosphorus nitride (γ-PN) monolayer as a candidate photocatalyst for reducing water into hydrogen and oxygen from the ...
DOI:
10.1039/c7ra10305k

19. Fluoromodules Consisting of a Promiscuous RNA Aptamer and Red or Blue Fluorogenic Cyanine Dyes: Selection, Characterization, and Bioimaging

Author:
Tan, Xiaohong,   et al.   ; Constantin, Tudor P.; Sloane, Kelly L.; Waggoner, Alan S.; Bruchez, Marcel P.; Armitage, Bruce A.;     Show all 6 Authors
Source:
Journal of the American Chemical Society 2017 v.139 no.26 pp. 9001-9009
ISSN:
1520-5126
Subject:
RNA; color; dyes; epidermal growth factor receptors; fluorescence; mammals; oligonucleotides; oxazoles; photostability; thiazoles
Abstract:
... An RNA aptamer selected for binding to the fluorogenic cyanine dye, dimethylindole red (DIR), also binds and activates another cyanine, oxazole thiazole blue (OTB), giving two well-resolved emission colors. The aptamer binds to each dye with submicromolar KD values, and the resulting fluoromodules exhibit fluorescence quantum yields ranging from 0.17 to 0.51 and excellent photostability. The aptam ...
DOI:
10.1021/jacs.7b04211

20. Effects of dietary leucine on growth performance, feed utilization, non-specific immune responses and gut morphology of juvenile golden pompano Trachinotus ovatus

Author:
Tan, Xiaohong,   et al.   ; Lin, Heizhao; Huang, Zhong; Zhou, Chuanpeng; Wang, Anli; Qi, Changle; Zhao, Shuyan;     Show all 7 Authors
Source:
Aquaculture 2016 v.465 pp. 100-107
ISSN:
0044-8486
Subject:
Trachinotus ovatus; adverse effects; antioxidant activity; blood serum; diet; enzyme activity; feed conversion; feed formulation; feed intake; fish; foregut; growth performance; hematocrit; hemoglobin; immune response; juveniles; leucine; lysozyme; malondialdehyde; microstructure; microvilli; midgut; protein deposition; protein efficiency ratio; proximate composition; specific growth rate; superoxide dismutase; weight gain
Abstract:
... The effects of dietary leucine on growth performance, feed utilization, non-specific immune responses and gut morphology were studied in juvenile golden pompano (Trachinotus ovatus) using 8-week feeding trials. Six isonitrogenous and isolipidic diets were formulated to contain six graded levels of dietary leucine (16.2, 20.9, 26.0, 30.7, 35.9 and 41.0gkg−1 leucine of dry diet). Each diet was rando ...
DOI:
10.1016/j.aquaculture.2016.08.034