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1. A TaqMan Real-Time PCR Assay for Apricot (Prunus armeniaca) as an Authenticity Test for Detection of Traces of Persipan in Marzipan

Author:
Taverniers, Isabel,   et al.   ; Van Gansbeke, Bart; Bény, Guido; De Loose, Marc;     Show all 4 Authors
Source:
Food analytical methods 2018 v.11 no.1 pp. 62-68
ISSN:
1936-9751
Subject:
Prunus armeniaca; Prunus dulcis; Prunus persica; almonds; analytical methods; apricots; detection limit; lipid transfer proteins; peaches; quantitative polymerase chain reaction
Abstract:
... Almond (Prunus dulcis), typically used in marzipan, is becoming more valuable by increasing global demand. As a consequence, substitution with cheaper alternatives is a common practice. Marzipan can be adulterated with apricot (Prunus armeniaca) or peach (Prunus persica), because of similar characteristics. Both species are closely related to almond; however, small differences in non-specific lipi ...
DOI:
10.1007/s12161-017-0964-5

2. Development of an LC-MS/MS method for the detection of traces of peanut allergens in chili pepper

Author:
Taverniers, Isabel,   et al.   ; Vandekerckhove, Marjolein; Van Droogenbroeck, Bart; De Loose, Marc; Daeseleire, Els; Gevaert, Philippe; Lapeere, Hilde; Van Poucke, Christof;     Show all 8 Authors
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.22 pp. 5201-5207
ISSN:
1618-2642
Subject:
allergens; detection limit; enzyme-linked immunosorbent assay; food matrix; foods; hot peppers; liquid chromatography; peanuts; peptides; portion size; reverse transcriptase polymerase chain reaction; risk; tandem mass spectrometry
Abstract:
... The recent detection of nuts (including peanut) in spices across the globe has led to enormous recalls of several spices and food products in the last two years. The lack of validated detection methods specific for spices makes it difficult to assess allergen presence at trace levels. Because of the urgent need for confirmation of possible peanut presence in chili peppers, an LC-MS/MS method was o ...
DOI:
10.1007/s00216-017-0506-4

3. Biotech rice: Current developments and future detection challenges in food and feed chain

Author:
Taverniers, Isabel,   et al.   ; Fraiture, Marie-Alice; Roosens, Nancy H.C.; De Loose, Marc; Deforce, Dieter; Herman, Philippe;     Show all 6 Authors
Source:
Trends in food science & technology 2016 v.52 pp. 66-79
ISSN:
0924-2244
Subject:
Agrobacterium radiobacter; biolistics; breeding methods; crops; gene expression; genetically modified organisms; genome walking; plant breeding; quantitative polymerase chain reaction; rice; traceability; transgenesis; Europe
Abstract:
... To improve agricultural practices and the food/feed security, plant breeding techniques were developed, including transgenesis commonly using Agrobacterium tumefaciens or biolistic technologies. To guarantee the traceability of GMO in food/feed chain and the consumer's freedom of choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementat ...
DOI:
10.1016/j.tifs.2016.03.011

4. Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products

Author:
Taverniers, Isabel,   et al.   ; Fraiture, Marie-Alice; Herman, Philippe; De Loose, Marc; Van Nieuwerburgh, Filip; Deforce, Dieter; Roosens, Nancy H.;     Show all 7 Authors
Source:
Food chemistry 2015 v.173 pp. 1259-1265
ISSN:
0308-8146
Subject:
DNA; detection limit; food matrix; food processing; genome walking; quantitative polymerase chain reaction; rice products; screening; transgenes; transgenic plants
Abstract:
... To identify unauthorised GMOs in food and feed matrices, an integrated approach has recently been developed targeting pCAMBIA family vectors, highly present in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently confirmed by characterising the transgene flanking regions, using DNA walking. Here, the DNA walking performance has been thoroughly tested for the fi ...
DOI:
10.1016/j.foodchem.2014.09.148

5. An innovative and integrated approach based on DNA walking to identify unauthorised GMOs

Author:
Taverniers, Isabel,   et al.   ; Fraiture, Marie-Alice; Herman, Philippe; De Loose, Marc; Deforce, Dieter; Roosens, Nancy H.;     Show all 6 Authors
Source:
Food chemistry 2014 v.147 pp. 60-69
ISSN:
0308-8146
Subject:
Bacillus thuringiensis; DNA; feeds; foods; genome; polymerase chain reaction; rice; transgenic plants
Abstract:
... In the coming years, the frequency of unauthorised genetically modified organisms (GMOs) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised GMOs containing a pCAMBIA family vector, frequently present in transgenic plants. This integrated approach is performed in two successive steps on Bt rice grains. Firs ...
DOI:
10.1016/j.foodchem.2013.09.112
PubMed:
24206686

6. Effect of oxidation in the presence or absence of lipids on hazelnut and soybean protein detectability by commercial ELISA

Author:
Taverniers, Isabel,   et al.   ; Platteau, Céline; Cucu, Tatiana; Devreese, Bart; De Loose, Marc; De Meulenaer, Bruno;     Show all 6 Authors
Source:
Food and agricultural immunology 2013 v.24 no.2 pp. 179-192
ISSN:
1465-3443
Subject:
allergens; enzyme-linked immunosorbent assay; food industry; food processing; foods; hazelnuts; lipids; oxidation; soy protein; soybeans
Abstract:
... Soybean and hazelnut represent major food allergens. Their inadvertent presence in foods might pose a serious threat to allergic consumers. Therefore, reliable detection methods are needed. Commercial enzyme-linked immunosorbent assays (ELISA) are the preferred methods especially by the food industry and enforcement agencies for the detection of contamination levels of food allergens. However, the ...
DOI:
10.1080/09540105.2012.677009

7. Effect of partial hydrolysis on the hazelnut and soybean protein detectability by ELISA

Author:
Taverniers, Isabel,   et al.   ; Cucu, Tatiana; Platteau, Céline; Devreese, Bart; De Loose, Marc; De Meulenaer, Bruno;     Show all 6 Authors
Source:
Food control 2013 v.30 no.2 pp. 497-503
ISSN:
0956-7135
Subject:
allergens; cross contamination; enzyme-linked immunosorbent assay; food allergies; food safety; foods; hazelnuts; hydrolysis; pepsin; polyacrylamide gel electrophoresis; protein hydrolysates; soy protein; soybeans
Abstract:
... Food allergies represent an increasing food safety problem. Soybean and hazelnut are identified as major food allergens and were selected for this study. During production of the hypo-allergenic formulae or other food products cross-contamination may occur. It is therefore important to have reliable analytical methods able to detect traces of allergenic proteins even after they have undergone part ...
DOI:
10.1016/j.foodcont.2012.07.017

8. Detection of Allergenic Ingredients Using Real-Time PCR: A Case Study on Hazelnut (Corylus avellena) and Soy (Glycine max)

Author:
Taverniers, Isabel,   et al.   ; Platteau, Céline; Loose, Marc de; Meulenaer, Bruno de;     Show all 4 Authors
Source:
Journal of agricultural and food chemistry 2011 v.59 no.20 pp. 10803-10814
ISSN:
0021-8561
Subject:
Corylus; Glycine max; allergenicity; allergens; case studies; compliance; cross reaction; foods; hazelnuts; ingredients; laws and regulations; quantitative polymerase chain reaction; risk
Abstract:
... Compliance with the European allergen labeling legislation (Directive 2007/68/EC) is only possible when coupled with appropriate methods to detect allergens in food. The aim of the current study was to develop new real-time PCR assays for the detection of hazelnut and soy and evaluate these assays via comparison with commercially available kits. Although the new assays were not as sensitive as the ...
DOI:
10.1021/jf202110f
PubMed:
21888436

9. Quantitative Detection of Hazelnut (Corylus avellana) in Cookies: ELISA versus Real-Time PCR

Author:
Taverniers, Isabel,   et al.   ; Platteau, Céline; Loose, Marc de; Meulenaer, Bruno de;     Show all 4 Authors
Source:
Journal of agricultural and food chemistry 2011 v.59 no.21 pp. 11395-11402
ISSN:
0021-8561
Subject:
Corylus avellana; DNA; allergens; anaphylaxis; cookies; enzyme-linked immunosorbent assay; food industry; food processing; hazelnut oil; hazelnuts; ingredients; model food systems; quantitative polymerase chain reaction; sweets
Abstract:
... Hazelnuts (Corylus avellana) are used widely in the food industry, especially in confectionery, where they are used raw, roasted, or in a processed formulation (e.g., praline paste and hazelnut oil). Hazelnuts contain multiple allergenic proteins, which can induce an allergic reaction associated with symptoms ranging from mild irritation to life-threatening anaphylactic shock. To date, immunochemi ...
DOI:
10.1021/jf202167b
PubMed:
21942372

10. Assessment of Primer/Template Mismatch Effects on Real-Time PCR Amplification of Target Taxa for GMO Quantification

Author:
Taverniers, Isabel,   et al.   ; Ghedira, Rim; Papazova, Nina; Vuylsteke, Marnik; Ruttink, Tom; Loose, Marc De;     Show all 6 Authors
Source:
Journal of agricultural and food chemistry 2009 v.57 no.20 pp. 9370–9377
ISSN:
0021-8561
Subject:
Zea mays; quantitative analysis; genetically modified foods; transgenes; transgenic plants; polymerase chain reaction; corn; nucleotide sequences; detection; accuracy; single nucleotide polymorphism
Abstract:
... GMO quantification, based on real-time PCR, relies on the amplification of an event-specific transgene assay and a species-specific reference assay. The uniformity of the nucleotide sequences targeted by both assays across various transgenic varieties is an important prerequisite for correct quantification. Single nucleotide polymorphisms (SNPs) frequently occur in the maize genome and might lead ...
DOI:
10.1021/jf901976a
PubMed:
19778057

11. Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts

Author:
Taverniers, Isabel V.,   et al.   ; Platteau, Céline Marie France; Bridts, Chris H.; Daeseleire, Els A.; De Loose, Marc R.; Ebo, Didier G.;     Show all 6 Authors
Source:
Food analytical methods 2010 v.3 no.4 pp. 382-388
ISSN:
1936-9751
Subject:
Corylus avellana; allergenicity; allergens; basophils; defatting; hazelnuts; humans; patients; polyacrylamide gel electrophoresis; proteinase inhibitors; proteins; sodium dodecyl sulfate
Abstract:
... This paper investigates two methodological issues of hazelnut protein extraction: the use of protease inhibitors during protein extraction and the effect of defatting hazelnuts before protein extraction. Different protein extracts from hazelnuts were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting to evaluate the presence of allergens in the ...
DOI:
10.1007/s12161-010-9147-3

12. Molecular toolbox for the identification of unknown genetically modified organisms

Author:
Taverniers, Isabel,   et al.   ; Ruttink, Tom; Demeyer, Rolinde; Van Gulck, Elke; Van Droogenbroeck, Bart; Querci, Maddalena; De Loose, Marc;     Show all 7 Authors
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.6 pp. 2073-2089
ISSN:
1618-2642
Subject:
Arabidopsis thaliana; commercialization; fluorescence; genetically modified organisms; humans; laws and regulations; polymerase chain reaction; sampling; screening; traceability; transgenes
Abstract:
... Competent laboratories monitor genetically modified organisms (GMOs) and products derived thereof in the food and feed chain in the framework of labeling and traceability legislation. In addition, screening is performed to detect the unauthorized presence of GMOs including asynchronously authorized GMOs or GMOs that are not officially registered for commercialization (unknown GMOs). Currently, una ...
DOI:
10.1007/s00216-009-3287-6

13. Evaluation of the reliability of maize reference assays for GMO quantification

Author:
Taverniers, Isabel,   et al.   ; Papazova, Nina; Zhang, David; Gruden, Kristina; Vojvoda, Jana; Yang, Litao; Gašparič, Meti Buh; Blejec, Andrej; Fouilloux, Stephane; De Loose, Marc;     Show all 10 Authors
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.6 pp. 2189-2201
ISSN:
1618-2642
Subject:
Zea mays; alcohol dehydrogenase; assays; beta-fructofuranosidase; breeding; commercialization; corn; cultivars; genes; genetically modified organisms; nucleotide sequences; polymerase chain reaction; provenance; single nucleotide polymorphism; zein; Spain
Abstract:
... A reliable PCR reference assay for relative genetically modified organism (GMO) quantification must be specific for the target taxon and amplify uniformly along the commercialised varieties within the considered taxon. Different reference assays for maize (Zea mays L.) are used in official methods for GMO quantification. In this study, we evaluated the reliability of eight existing maize reference ...
DOI:
10.1007/s00216-009-3386-4

14. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines

Author:
Taverniers, Isabel,   et al.   ; Papazova, Nina; Windels, Pieter; Depicker, Ann; Roldan-Ruiz, Isabel; Milcamps, Anne; Van Bockstaele, Erik; Van Den Eede, Guy; De Loose, Marc;     Show all 9 Authors
Source:
Plant cell reports 2006 v.25 no.12 pp. 1362-1368
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; application methods; ecotypes; food labeling; loci; monitoring; plant tissues; tissue culture; traceability; transfer DNA; transgenes; transgenic plants
Abstract:
... The stability of the inserted transgenes and particularly the junction regions of transgenic events is a concern of food labeling, traceability and post release monitoring, as these regions are used for development of event-specific DNA-based detection methods. During the standard agricultural breeding practices, the transgenic lines can be exposed to completely different conditions than those in ...
DOI:
10.1007/s00299-006-0192-8
PubMed:
16810524

15. Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices

Author:
Taverniers, Isabel,   et al.   ; Papazova, Nina; Ghedira, Rim; Van Glabeke, Sabine; Bartegi, Aghleb; Windels, Pieter; Roldan-Ruiz, Isabel; Van Bockstaele, Erik; Milcamps, Anne; Van Den Eede, Guy; Depicker, Ann; De Loose, Marc;     Show all 12 Authors
Source:
Plant cell reports 2008 v.27 no.4 pp. 749-757
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; abiotic stress; capillary electrophoresis; genetic transformation; light intensity; nucleotide sequences; oxidative stress; photostability; restriction fragment length polymorphism; sequence analysis; transfer DNA; transgenes
Abstract:
... Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of ...
DOI:
10.1007/s00299-007-0495-4
PubMed:
18087701

16. Validation of the performance of a GMO multiplex screening assay based on microarray detection

Author:
Taverniers, Isabel,   et al.   ; Leimanis, Serge; Hamels, Sandrine; Nazé, Florence; Mbongolo Mbella, Guillaume; Sneyers, Myriam; Hochegger, Rupert; Broll, Hermann; Roth, Lillian; Dallmann, Klára; Micsinai, Adrienn; La Paz, José Luis; Pla, Maria; Brünen-Nieweler, Claudia; Papazova, Nina; Hess, Norbert; Kirschneit, Britta; Bertheau, Yves; Audeon, Colette; Laval, Valérie; Busch, Ulrich; Pecoraro, Sven; Neumann, Katrin; Rösel, Sibylle; van Dijk, Jeroen; Kok, Esther; Bellocchi, Gianni; Foti, Nicoletta; Mazzara, Marco; Moens, William; Remacle, José; Van Den Eede, Guy;     Show all 32 Authors
Source:
European food research & technology 2008 v.227 no.6 pp. 1621-1632
ISSN:
1438-2377
Subject:
DNA; food research; genetically modified organisms; hybridization; microarray technology; polymerase chain reaction; screening; Germany
Abstract:
... A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip® GMO, Eppendorf, Germany). The v ...
DOI:
10.1007/s00217-008-0886-y