You searched for:
Author
"Timothy A. Reinhardt"
Remove constraint Author: "Timothy A. Reinhardt"
PubAg
Main content area
Limit your search
Search
26 Search Results
« Previous |
1 - 20 of 26
|
Next »
Search Results
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Ellie J. Putz; Eduardo Casas; Wanda J. Weber; Brian A. Crooker; Show all 6 Authors
- Source:
- Journal of dairy science 2022 v.105 no.6 pp. 5435-5448
- ISSN:
- 0022-0302
- Subject:
- Escherichia coli; Holstein; blood serum; body temperature; gene expression regulation; genotype; herds; immune response; innate immunity; interleukin-6; mammary glands; milk; milk yield; neutrophils; somatic cell count; transcriptome; whey; Minnesota
- Abstract:
- ... Selective breeding of US dairy cows since the mid-1960s has contributed to remarkable gains in milk yield per cow. This increased milk yield has been associated with an increase in health issues. Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holsteins (CH) has dem ...
- Handle:
- 10113/7752301
- DOI:
- 10.3168/jds.2021-21166
- https://doi.org/10.3168/jds.2021-21166
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Show all 2 Author
- Source:
- Data in Brief 2020 v.31 no. pp. 105954
- ISSN:
- 2352-3409
- Subject:
- cattle; data collection; lactation; liquid chromatography; mammary glands; milk; proteome; secretion; tandem mass spectrometry
- Abstract:
- ... This dataset is a label-free quantitation of proteins milk and dry secretions from the end of lactation through day 21 of the dry period using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The data supplied in this article supports the accompanying publication entitled “Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through da ...
- DOI:
- 10.1016/j.dib.2020.105954
- CHORUS:
- 10.1016/j.dib.2020.105954
- https://doi.org/10.1016/j.dib.2020.105954
- Author:
- Timothy A. Reinhardt, et al. ; Rohana P. Dassanayake; Taylor L. T. Wherry; Shollie M. Falkenberg; Eduardo Casas; Judith R. Stabel; Show all 6 Authors
- Source:
- BMC veterinary research 2021 v.52 no.1 pp. 11
- ISSN:
- 1297-9716
- Subject:
- Mycobacterium avium subsp. paratuberculosis; antibacterial properties; antimicrobial peptides; cattle; cell membranes; cytosol; macrophages; natural killer cells; paratuberculosis
- Abstract:
- ... Infection with Mycobacterium avium subspecies paratuberculosis (MAP) is complex, but little is known about the role that natural killer (NK) cells play. In the present study, four bovine NK-lysin peptides were synthesized to evaluate their bactericidal activity against MAP. The results demonstrated that bNK-lysin peptides were directly bactericidal against MAP, with bNK1 and bNK2A being more poten ...
- DOI:
- 10.1186/s13567-021-00893-2
- https://doi.org/10.1186/s13567-021-00893-2
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Show all 2 Author
- Source:
- Journal of proteomics 2020 v.223 pp. 103831
- ISSN:
- 1874-3919
- Subject:
- antimicrobial proteins; bacterial growth; coliform bacteria; dairy cows; gene expression regulation; gene ontology; immune response; lactation; lactoferrin; mammary glands; mass spectrometry; mastitis; milk; pathogens; protein content; proteome; secretion; whey protein
- Abstract:
- ... The dry period in dairy cows is critical to mammary health. We used label-free mass spectroscopy to quantify whey proteins from milk/dry secretions collected on days 0, 3,10, and 21 of the dry period. The number of proteins identified was 776. The number of proteins upregulated at one or more times in the dry period was 109. The most significant enriched Gene Ontology (GO) terms for the upregulate ...
- Handle:
- 10113/6963593
- DOI:
- 10.1016/j.jprot.2020.103831
- https://doi.org/10.1016/j.jprot.2020.103831
- Author:
- Timothy A. Reinhardt, et al. ; Ellie J. Putz; Mitchell V. Palmer; Hao Ma; Eduardo Casas; John D. Lippolis; Show all 6 Authors
- Source:
- BMC veterinary research 2020 v.16 no.1 pp. 336
- ISSN:
- 1746-6148
- Subject:
- Gram-positive bacteria; Holstein; Staphylococcus aureus; abscess; antibiotics; calving; case studies; connective tissues; dairy cows; epithelium; granulocyte colony-stimulating factor; granuloma; infection; lactation; mastitis; mechanics; milk; myeloperoxidase; necropsy; neutrophils; signs and symptoms (animals and humans); somatic cell count; traps; veterinary medicine
- Abstract:
- ... BACKGROUND: Mastitis is the most common health concern plaguing the modern dairy cow and costs dairy producers estimates of two billion dollars annually. Staphylococcus aureus infections are prevalent, displaying varied disease presentation and markedly low cure rates. Neutrophils are considered the first line of defense against mastitis causing bacteria and are frequently targeted in the developm ...
- Handle:
- 10113/7110991
- DOI:
- 10.1186/s12917-020-02528-8
- https://doi.org/10.1186/s12917-020-02528-8
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Devin B. Holman; Brian W. Brunelle; Tyler C. Thacker; Bradley L. Bearson; Randy E. Sacco; Thomas A. Casey; Show all 8 Authors
- Source:
- Infection and immunity 2018 v.86 no.1 pp. e00566-17
- ISSN:
- 0019-9567
- Subject:
- DNA; Escherichia coli; bacteria; bacterial motility; bovine mastitis; chronic diseases; complement; dairy cattle; gene expression; genomics; operon; phenotype; sequence analysis; transcriptomics
- Abstract:
- ... Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. It is most often transient in nature, causing an infection that lasts 2 to 3 days. However, E. coli has been shown to cause a persistent infection in a minority of cases. Mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine differences between E. coli stra ...
- Handle:
- 10113/6564235
- DOI:
- 10.1128/IAI.00566-17
- PubMed:
- 29061709
- PubMed Central:
- PMC5736815
- https://doi.org/10.1128/IAI.00566-17
- Author:
- Timothy A. Reinhardt, et al. ; Brandie M. Cross; Gerda E. Breitwieser; Rajini Rao; Show all 4 Authors
- Source:
- American Journal of Physiology - Cell Physiology 2014 v.306 no.6 pp. C515
- ISSN:
- 0363-6143
- Subject:
- apoptosis; blood; breast neoplasms; breasts; calcium; carcinogenesis; cell movement; cell physiology; cell proliferation; epithelium; gene expression; genes; lactation; metastasis; milk; mortality; neoplasm cells; radiography; secretion; transporters; women; United States
- Abstract:
- ... Breast cancer is the second leading cause of cancer mortality in women, estimated at nearly 40,000 deaths and more than 230,000 new cases diagnosed in the U.S. this year alone. One of the defining characteristics of breast cancer is the radiographic presence of microcalcifications. These palpable mineral precipitates are commonly found in the breast after formation of a tumor. Since free Ca2+ play ...
- Handle:
- 10113/61332
- DOI:
- 10.1152/ajpcell.00330.2013
- PubMed:
- 24225884
- PubMed Central:
- PMC3948975
- https://doi.org/10.1152/ajpcell.00330.2013
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Randy E. Sacco; Show all 3 Authors
- Source:
- Biochemical and Biophysical Research Communications 2014 v.445 no.2 pp. 417-421
- ISSN:
- 0006-291X
- Subject:
- Golgi apparatus; adenosinetriphosphatase; antiporters; calcium; cells; exhibitions; knockout mutants; lactation; mammary glands; milk; plasma membrane; pregnancy
- Abstract:
- ... Plasma membrane Ca(2+)-ATPase 2 (PMCA2) knockout mice showed that ~ 60 % of calcium in milk is transported across the mammary cells apical membrane by PMCA2. The remaining milk calcium is thought to arrive via the secretory pathway through the actions of secretory pathway Ca(2+)-ATPase’s 1 and/or 2 (SPCA1 and 2). However, another secretory pathway calcium transporter was recently described. The qu ...
- Handle:
- 10113/61347
- DOI:
- 10.1016/j.bbrc.2014.02.020
- https://doi.org/10.1016/j.bbrc.2014.02.020
- Author:
- Timothy A. Reinhardt, et al. ; Fernando L. Leite; John P. Bannantine; Judith R. Stabel; Show all 4 Authors
- Source:
- Veterinary microbiology 2015 v.175 no.2-4 pp. 275-285
- ISSN:
- 0378-1135
- Subject:
- protein synthesis; antigens; T-lymphocytes; interferon-gamma; energy metabolism; epithelial cells; polyacrylamide gel electrophoresis; recombinant proteins; paratuberculosis; Western blotting; succinate dehydrogenase (quinone); membrane proteins; excision; cows; mass spectrometry; Mycobacterium tuberculosis; Mycobacterium avium subsp. paratuberculosis; cysteine; pathogens
- Abstract:
- ... Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic enteric disease of ruminant animals. In the present study, blue native PAGE electrophoresis and 2D SDS-PAGE were used to separate MAP envelope protein complexes, followed by mass spectrometry (MS) to identify individual proteins within the complexes. Identity of individual proteins within complex ...
- Handle:
- 10113/60768
- DOI:
- 10.1016/j.vetmic.2014.11.009
- https://doi.org/10.1016/j.vetmic.2014.11.009
- Author:
- Timothy A. Reinhardt, et al. ; Randal T. Capsel; Charles O. Thoen; John D. Lippolis; Renee Olsen; Judith R. Stabel; John P. Bannantine; Show all 7 Authors
- Source:
- Plos One 2016 v.11 no.5 pp. e0154685
- ISSN:
- 1932-6203
- Subject:
- Escherichia coli; Mycobacterium avium subsp. paratuberculosis; antibodies; antigens; autoclaving; blood; erythema; filtrates; filtration; guinea pigs; immunoblotting; interferon-gamma; mass spectrometry; polyacrylamide gel electrophoresis; recombinant proteins
- Abstract:
- ... Mycobacterium avium subsp. paratuberculosis (MAP) purified protein derivatives (PPDs) are immunologic reagents prepared from cultured filtrates of the type strain. Traditional production consists of floating culture incubation at 37°C, organism inactivation by autoclaving, coarse filtration, and protein precipitation. Three traditional production PPDs were used in this study including lot 9801, wh ...
- Handle:
- 10113/62940
- DOI:
- 10.1371/journal.pone.0154685
- PubMed:
- 27136199
- PubMed Central:
- PMC4852940
- CHORUS:
- 10.1371/journal.pone.0154685
- https://doi.org/10.1371/journal.pone.0154685
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Brian W. Brunelle; Randy E. Sacco; Tyler C. Thacker; Torey P. Looft; Thomas A. Casey; Show all 7 Authors
- Source:
- mSystems 2016 v.1 no.4 pp. -
- ISSN:
- 2379-5077
- Subject:
- Escherichia coli; bacteria; bacterial motility; chelation; chronic diseases; culture media; dairy cattle; gene expression; gene expression regulation; genes; iron; phenotype; sequence analysis; swimming; transcriptome; transcriptomics; virulence
- Abstract:
- ... Escherichia coli is a leading cause of intramammary infections in dairy cattle and is typically transient in nature. However, in a minority of cases, E. coli can cause persistent infections. Although the mechanisms that allow for a persistent intramammary E. coli infection are not fully understood, we have previously shown that proficient bacterial motility (swimming and swarming) in vitro is corr ...
- Handle:
- 10113/5498088
- DOI:
- 10.1128/mSystems.00064-16
- PubMed:
- 27822550
- PubMed Central:
- PMC5072449
- https://doi.org/10.1128/mSystems.00064-16
- Author:
- Timothy A. Reinhardt, et al. ; Fernando L. Leite; Livia B. Eslabão; Bruce Pesch; John P. Bannantine; Judith R. Stabel; Show all 6 Authors
- Source:
- Veterinary immunology and immunopathology 2015 v.167 no.1-2 pp. 15-21
- ISSN:
- 0165-2427
- Subject:
- CD4-positive T-lymphocytes; Mycobacterium avium subsp. paratuberculosis; concanavalin A; cows; cytotoxicity; disease course; flow cytometry; humoral immunity; paratuberculosis; phenotype; phosphorylation
- Abstract:
- ... Paratuberculosis is a chronic intestinal disease of ruminant animals caused by Mycobacterium avium subsp. paratuberculosis (MAP). A hallmark of paratuberculosis is a transition from a cell-mediated Th1 type response to a humoral Th2 response with the progression of disease from a subclinical to clinical state. The objective of this study was to investigate the expression of two crucial molecules i ...
- DOI:
- 10.1016/j.vetimm.2015.06.017
- https://doi.org/10.1016/j.vetimm.2015.06.017
- Author:
- Timothy A. Reinhardt, et al. ; Brandie M. Cross; Anniesha Hack; Rajini Rao; Alexander G. Obukhov; Show all 5 Authors
- Source:
- PloS one v.8 no.6 pp. -
- ISSN:
- 1932-6203
- Subject:
- Ca2-transporting ATPase; basement membrane; breast neoplasms; calcium; epithelial cells; ion transport; lactation; mammary glands; mice; milk secretion; models; plasma membrane; prolactin
- Abstract:
- ... An unconventional interaction between SPCA2, an isoform of the Golgi secretory pathway Ca 2+ -ATPase, and the Ca 2+ influx channel Orai1, has previously been shown to contribute to elevated Ca 2+ influx in breast cancer derived cells. In order to investigate the physiological role of this interaction, we examined expression and localization of SPCA2 and Orai1 in mouse lactating mammary glands. We ...
- Handle:
- 10113/57302
- DOI:
- 10.1371/journal.pone.0067348
- PubMed:
- 23840669
- PubMed Central:
- PMC3696057
- https://doi.org/10.1371/journal.pone.0067348
- Author:
- Timothy A. Reinhardt, et al. ; Jiarong Li; Aimée-Lee Luco; Benoît Ochietti; Ibtihal Fadhil; Anne Camirand; René St-Arnaud; William Muller; Richard Kremer; Show all 9 Authors
- Source:
- Endocrinology 2016 v.157 no.6 pp. 2204-2216
- ISSN:
- 0013-7227
- Subject:
- angiogenesis; animal models; apoptosis; breast neoplasms; carcinogenesis; cell cycle; cell proliferation; cytoplasm; disease course; endocrinology; epithelial cells; epithelium; genes; integrins; kidneys; mammary glands; mammary neoplasms (animal); mice; neoplasm cells; phosphorylation; signal transduction; transcription factor NF-kappa B; vitamin D; vitamin status
- Abstract:
- ... Biologically-active vitamin D (1,25(OH)2D) is synthetized from inactive prohormone 25(OH)D by the enzyme CYP27B1 1-a-hydroxylase in kidney and several extra-renal tissues including breast. While the development of breast cancer has been linked to inadequate vitamin D status, the importance of bioactive vitamin D production within tumors themselves is not fully understood. To investigate the role o ...
- Handle:
- 10113/62884
- DOI:
- 10.1210/en.2015-1824
- https://doi.org/10.1210/en.2015-1824
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Brian J. Nonnecke; Randy E. Sacco; Show all 4 Authors
- Source:
- Journal of proteomics 2012 v.75 no.5 pp. 1486-1492
- ISSN:
- 1874-3919
- Subject:
- milk secretion; xanthine oxidase; fluid milk; chromatography; milk; dairy cows; proteome; trypsin; lactadherin; protein composition; lactation stage; adipophilin; milk fat; fat globules; spectrometers; exosomes; milk production; pH; butyrophilin
- Abstract:
- ... Exosomes are 40–100nm membrane vesicles of endocytic origin, secreted by cells and are found in biological fluids including milk. These exosomes are extracellular organelles important in intracellular communication, and immune function. Therefore, the proteome of bovine milk exosomes may provide insight into the complex processes of milk production. Exosomes were isolated from the milk of mid-lact ...
- Handle:
- 10113/54045
- DOI:
- 10.1016/j.jprot.2011.11.017
- https://doi.org/10.1016/j.jprot.2011.11.017
- Author:
- Timothy A. Reinhardt, et al. ; Randy E. Sacco; Brian J. Nonnecke; John D. Lippolis; Show all 4 Authors
- Source:
- Journal of Proteomics 2013 v.82 pp. 141-154
- ISSN:
- 1874-3919
- Subject:
- Staphylococcus aureus; proteome; protein synthesis; milk proteins; exosomes; mammary gland function; neutrophils; milk; whey; milk fat; cows; bovine mastitis; protein composition
- Abstract:
- ... Milk protein expression in healthy cows and cows with mastitis will provide information important for the dairy food industry and immune function in the mammary gland. To facilitate protein discovery, milk was fractioned into whey, milk fat globule membranes (MFGM) and exosomes from healthy and Staphylococcus aureus infected cows. Amine-reactive isobaric tags (iTRAQ) were used to quantify protein ...
- Handle:
- 10113/61334
- DOI:
- 10.1016/j.jprot.2013.02.013
- https://doi.org/10.1016/j.jprot.2013.02.013
- Author:
- Timothy A. Reinhardt, et al. ; Corwin D. Nelson; John D. Lippolis; Randy E. Sacco; Jessi L. Powell; Mary E. Drewnoski; Matthew O’Neil; Donald C. Beitz; William P. Weiss; Show all 9 Authors
- Source:
- Journal of dairy science 2016 v.99 no.12 pp. 10150-10160
- ISSN:
- 0022-0302
- Subject:
- blood serum; calves; cholecalciferol; cows; dairy cattle; dairy industry; early lactation; heifers; herds; late lactation; metabolites; milk; milk replacer; neonates; nutritionists; pasteurization; statistical analysis; summer; vitamin status; vitamin supplements; wastes; yearlings; United States
- Abstract:
- ... The need for vitamin D supplementation of dairy cattle has been known for the better part of the last century and is well appreciated by dairy producers and nutritionists. Whether current recommendations and practices for supplemental vitamin D are meeting the needs of dairy cattle, however, is not well known. The vitamin D status of animals is reliably indicated by the concentration of the 25-hyd ...
- Handle:
- 10113/5564784
- DOI:
- 10.3168/jds.2016-11727
- https://doi.org/10.3168/jds.2016-11727
- Author:
- Timothy A. Reinhardt, et al. ; John D. Lippolis; Brian W. Brunelle; Randy E. Sacco; Brian J. Nonnecke; Belgin Dogan; Kenneth Simpson; Ynte H. Schukken; Show all 8 Authors
- Source:
- Journal of proteomics 2014 v.108 pp. 373-381
- ISSN:
- 1874-3919
- Subject:
- Escherichia coli; assays; bacterial motility; chronic diseases; dairy cattle; infection; mastitis; phenotype; protein synthesis; proteins; proteomics; strains; swarming
- Abstract:
- ... Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. Typically this infection is transient in nature, causing an infection that lasts 2–3 days. However, in a minority of cases, E. coli has been shown to cause a persistent infection. The mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine protein expression ...
- Handle:
- 10113/61261
- DOI:
- 10.1016/j.jprot.2014.06.008
- https://doi.org/10.1016/j.jprot.2014.06.008
- Author:
- Timothy A. Reinhardt, et al. ; Lionel Rossdeutscher; Jiarong Li; Aimee-Lee Luco; Ibtihal Fadhil; Benoit Ochietti; Anne Camirand; Dao Chao Huang; William Muller; Richard Kremer; Show all 10 Authors
- Source:
- Cancer prevention research 2015 v.8 no.2 pp. 120-128
- ISSN:
- 1940-6207
- Subject:
- 25-hydroxycholecalciferol; animal models; breast neoplasms; calcitriol; chemoprevention; diet; hypercalcemia; mammary neoplasms (animal); metabolites; metastasis; mice; neoplasm cells; vitamin status; women
- Abstract:
- ... Development of oncologic conditions is often linked to inadequate vitamin D status. The chemoprevention ability of this molecule is of high interest for breast cancer, the most common malignancy in women worldwide. Current effective vitamin D analogs including the naturally occurring active metabolite 1,25-dihydroxycholecalciferol (1,25(OH)2D) frequently cause hypercalcemia at pharmacological dose ...
- Handle:
- 10113/61267
- DOI:
- 10.1158/1940-6207.CAPR-14-0110
- https://doi.org/10.1158/1940-6207.CAPR-14-0110
- Author:
- Timothy A. Reinhardt, et al. ; Corwin D. Nelson; Donald C. Beitz; John D. Lippolis; Derya Unutmaz; Show all 5 Authors
- Source:
- PloS one v.5 no.11 pp. -
- ISSN:
- 1932-6203
- Subject:
- dairy cows; innate immunity; 25-hydroxycholecalciferol; mammary glands; oxygenases; macrophages; gene expression; calcitriol; genes; Streptococcus uberis; in vitro studies; enzyme activity; gene expression regulation; bovine mastitis; pathogens; Toll-like receptors
- Abstract:
- ... Numerous in vitro studies have shown that toll-like receptor signaling induces 25-hydroxyvitamin D 3 1α-hydroxylase (1α-OHase; CYP27B1) expression in macrophages from various species. 1α-OHase is the primary enzyme that converts 25-hydroxyvitamin D 3 to 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3). Subsequently, synthesis of 1,25(OH) 2 D 3 by 1α-OHase in macrophages has been shown to modulate innate ...
- Handle:
- 10113/47708
- DOI:
- 10.1371/journal.pone.0015469
- PubMed:
- 21124742
- PubMed Central:
- PMC2993969
- https://doi.org/10.1371/journal.pone.0015469