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Tobaccomosaicvirus, etc ; azides; biosensors; detection limit; electrochemiluminescence; electrodes; gold; polymerization; polymers; Show all 9 Subjects
Abstract:
... Herein, an electroluminescence (ECL) biosensor was constructed by combining click chemistry with activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP) to sensitively assay tobacco mosaic virus (TMV) RNA for the first time. First, hairpin DNA (hDNA) was self-assembled on the gold electrode surface through Au–S bonding. The hDNA hybridized with the tDNA to for ...
Tobaccomosaicvirus, etc ; DNA; Tomato mottle virus; biotechnology; humans; insects; microRNA; mutational analysis; nucleotides; purines; Show all 10 Subjects
Abstract:
... Artificial microRNA (amiRNA) technology has allowed researchers to direct efficient silencing of specific transcripts using as few as 21 nucleotides (nt). However, not all the artificially designed amiRNA constructs result in selection of the intended ~21‐nt guide strand amiRNA. Selection of the miRNA guide strand from the mature miRNA duplex has been studied in detail in human and insect systems, ...
Tobaccomosaicvirus, etc ; antiviral properties; enzyme activity; limonoids; pathogens; pesticides; physiology; systemic acquired resistance; tobacco; Show all 9 Subjects
Abstract:
... Three prieurianin-type limonoids (1–3), including two new compounds (1 and 2) and one known compound (3) were isolated from Munronia henryi. These compounds were tested for their activities against tobacco mosaic virus (TMV) by the conventional half-leaf method and the outcomes were analyzed by western blotting and RT-PCR assays. The three tested compounds, at 100 μg/mL, showed strong antiviral ac ...
Tobaccomosaicvirus, etc ; adhesion; air; computer software; crystallization; humans; metrology; potatoes; topography; vaccines; vertebrate viruses; viruses; Show all 12 Subjects
Abstract:
... Scanning probe microscopy is a group of measurements that provides 3D visualization of viruses in different environmental conditions including liquids and air. Besides 3D topography it is possible to measure the properties like mechanical rigidity and stability, adhesion, tendency to crystallization, surface charge, etc. Choosing the right substrate and scanning parameters makes it much easier to ...
... The diagnosis of virus infection can facilitate the effective control of plant viral diseases. To date, serological and molecular methods for the detection of virus infection have been widely used, but these methods have disadvantages if applied for broad-range and large-scale detection. Here, we investigated the effect of infection of several different plant RNA and DNA viruses such as cucumber m ...
Tobaccomosaicvirus, etc ; biocompatible materials; cell cycle checkpoints; fibroins; gelatin; glycerol; humans; integrins; silk; surface roughness; viruses; water solubility; water vapor; Show all 13 Subjects
Abstract:
... Flexible films were prepared from silk fibroin (SF) and gelatin (GA) with a presence of glycerol (Gly), followed by water vapor annealing to achieve water-insoluble matrices. The blended SF/GA/Gly films were chemically conjugated with tobacco mosaic virus (TMV), either native (TMV-wt) or genetically modified with Arg-Gly-Asp (RGD) sequences (TMV-rgd), to improve cellular responses. The attachment ...
Tobaccomosaicvirus, etc ; antifungal properties; antiviral properties; coat proteins; drug resistance; food chemistry; fungi; mechanism of action; pesticides; plant protection; sesquiterpenoids; Show all 11 Subjects
Abstract:
... The unreasonable use or long-term use of a single variety of pesticide has led to drug resistance and made the pesticides ineffective. Therefore, the creation of new, efficient, and low-risk pesticides is imminent. Marine natural products play a vital role in serving as new lead compounds. In this work, we realized the efficient preparation of nine marine sesquiterpenes with the Stork–Danheiser re ...
Tobaccomosaicvirus, etc ; antiviral properties; coat proteins; cross resistance; mechanism of action; median effective concentration; pest management; plant protection; thiourea; viruses; Show all 10 Subjects
Abstract:
... BACKGROUND: Devastating plant virus diseases leading to bad harvests and lower quality of crops have made feeding the beyond seven billion population a huge challenge. Nevertheless, growing resistance and cross resistance of crop protection agents have made this challenge harder. Therefore, an efficient crop protection agent with novel structure and mode of action showing higher efficiency and eco ...
Tobaccomosaicvirus, etc ; Alfalfa mosaic virus; Cucumber mosaic virus; Cuscuta campestris; Tomato yellow leaf curl virus; growth and development; parasitic plants; Bulgaria; Show all 8 Subjects
Abstract:
... Members of the genus Cuscuta or dodders represent widely distributed holoparasitic flowering plants with large agricultural and ecological significance. Besides their direct negative impact on the growth and development of host plants, they are also regarded as putative vectors of various pathogens, including plant viruses. In the present study, a total of 36 populations, belonging to 4 Cuscuta sp ...
Tobaccomosaicvirus, etc ; Physalospora; Thanatephorus cucumeris; allelochemicals; antibiotics; antiviral agents; cytosine; fluorescence; food chemistry; hydrogen; mechanism of action; nucleosides; phytoalexins; protective effect; titration; viruses; Show all 16 Subjects
Abstract:
... In response to the invasion of plant viruses and pathogenic fungi, higher plants produce defensive allelochemicals. Finding candidate varieties of botanical pesticides based on allelochemicals is one of the important ways to create efficient and green pesticides. Here, a series of camalexin derivatives based on a phytoalexin camalexin scaffold were designed, synthesized, and assessed for their ant ...
Tobaccomosaicvirus, etc ; Meloidogyne incognita; catechol oxidase; host range; human health; morphogenesis; nematicides; phenol; plant pathology; protein content; root galls; root-knot nematodes; soil treatment; tomatoes; Show all 14 Subjects
Abstract:
... Root-knot nematodes (Meloidogyne spp.) have been reported to be responsible for large economic losses of agricultural crops due to their wide host range and variety of suitable climates. The control measures of these parasitic nematodes depend upon synthetic nematicides and a small number bio-based products. Chemical nematicides are eliciting adverse effects on the environment and human health. In ...
... Reactions of plants in 173 wild tomato accessions belonging to Solanum habrochaites and S. peruvianum were studied by inoculation with a tobamovirus, tomato brown rugose fruit virus (ToBRFV). Around 10–50% of plants in nine accessions of S. habrochaites and one of S. peruvianum were demonstrated to be highly resistant. Resistant plants showed no symptoms at 22–24 °C, and no virus could be detected ...
... Stomatal movement participates in plant immunity by directly affecting the invasion of bacteria, but the genes that regulate stomatal immunity have not been well identified. Here, we characterised the function of the bZIP59 transcription factor from Arabidopsis thaliana, which is constitutively expressed in guard cells. The bzip59 mutant is partially impaired in stomatal closure induced by Pseudom ...
... BACKGROUND: Early, precise and simultaneous identification of plant viruses is of great significance for preventing virus spread and reducing losses in agricultural yields. METHODS AND RESULTS: In this study, the identification of plant viruses from symptomatic samples collected from a cigar tobacco planting area in Deyang and a flue-cured tobacco planting area in Luzhou city, Sichuan Province, Ch ...
Tobaccomosaicvirus, etc ; RNA; capsid; models; satellites; viral genome; Show all 6 Subjects
Abstract:
... Three-dimensional RNA domain reconstruction is important for the assembly, disassembly and delivery functionalities of a packed proteinaceus capsid. However, to date, the self-association of RNA molecules is still an open problem. Recent chemical probing reports provide, with high reliability, the secondary structure of diverse RNA ensembles, such as those of viral genomes. Here, we present a meth ...
Tobaccomosaicvirus, etc ; Streptomyces; alkalinization; callose; disease resistance; gas chromatography-mass spectrometry; gel chromatography; hypersensitive response; methylation; nitric oxide; nuclear magnetic resonance spectroscopy; pathogens; programmed cell death; salicylic acid; tobacco; Show all 15 Subjects
Abstract:
... EPS66A was derived from an unidentified Streptomyces sp. HL-66 by chemical fraction and disease-resistance assays. It was identified as a polysaccharide through a series of chemical characterization, including infrared spectrum analysis, methylation, gas chromatography–mass spectrometry, nuclear magnetic resonance, and high-performance gel permeation chromatography. To determine its effect in plan ...
... Valsa canker, caused by Valsa pyri (Vp), is a necrotrophic fungal disease that seriously damaged the pear industry in the world. Investigations on resistant mechanisms are helpful for resistance breeding and effective control methods. The current study obtained suspension cells of a resistant rootstock ‘Duli-G03’ (Pyrus betulifolia bunge) and a susceptible species ‘Yuluxiang’ (Pyrus bretschneideri ...
... Litsea cubeba essential oil (LCO) has a wide range of industrial uses. This study found that LCO has significant anti-TMV activity and that its active components are monoterpenoids. Citral, the main active component of the oil, showed outstanding anti-TMV activity. The protective, inactive, and curative efficacies of citral (500 μg/mL) against TMV were 76.27%, 55.14%, and 50.42%, respectively, whi ...
... BACKGROUND: Plant elicitors are a class of plant protection agents that can stimulate plant immunity against phytopathogen without a potential resistance problem. In searching for novel plant elicitor candidates, a series of novel N‐(2‐phenyl‐3‐pyridyl) thiadiazole/isothiazole carboxamide analogs were designed and synthesized. RESULTS: In vitro bioassay showed that all new compounds exhibited weak ...
Tobaccomosaicvirus, etc ; Nicotiana benthamiana; Tomato mosaic virus; ammonium; asparagine; aspartate-ammonia ligase; aspartic acid; cytosol; fluorescence; fluorescent proteins; glutamine; immunity; luciferase; plant pathology; precipitin tests; salicylic acid; tobacco; two hybrid system techniques; Show all 18 Subjects
Abstract:
... Asparagine synthetase is a key enzyme that catalyses the conversion of amide groups from glutamine or ammonium to aspartate, which leads to the generation of asparagine. However, the role of asparagine synthetase in plant immunity remains largely unknown. Here, we identified a Nicotiana benthamiana asparagine synthetase B (NbAS‐B) that associates with tomato mosaic virus coat protein‐interacting p ...