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1. Construction of electrochemiluminescence biosensor via click chemistry and ARGET-ATRP for detecting tobacco mosaic virus RNA

Author:
Xiaoyu Guo; Luyao Chen; Peipei Li; Xiaofei Li; Jianfeng Wang; Liang Guo; Huaixia Yang
Source:
Analytical biochemistry 2022 pp. 114834
ISSN:
0003-2697
Subject:
Tobacco mosaic virus,   etc   ; azides; biosensors; detection limit; electrochemiluminescence; electrodes; gold; polymerization; polymers;     Show all 9 Subjects
Abstract:
... Herein, an electroluminescence (ECL) biosensor was constructed by combining click chemistry with activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP) to sensitively assay tobacco mosaic virus (TMV) RNA for the first time. First, hairpin DNA (hDNA) was self-assembled on the gold electrode surface through Au–S bonding. The hDNA hybridized with the tDNA to for ...
DOI:
10.1016/j.ab.2022.114834

2. Artificial microRNA guide strand selection from duplexes with no mismatches shows a purine‐rich preference for virus‐ and non‐virus‐based expression vectors in plants

Author:
Yen‐Wen Kuo; Bryce W. Falk
Source:
Plant biotechnology journal 2022 v.20 no.6 pp. 1069-1084
ISSN:
1467-7644
Subject:
Tobacco mosaic virus,   etc   ; DNA; Tomato mottle virus; biotechnology; humans; insects; microRNA; mutational analysis; nucleotides; purines;     Show all 10 Subjects
Abstract:
... Artificial microRNA (amiRNA) technology has allowed researchers to direct efficient silencing of specific transcripts using as few as 21 nucleotides (nt). However, not all the artificially designed amiRNA constructs result in selection of the intended ~21‐nt guide strand amiRNA. Selection of the miRNA guide strand from the mature miRNA duplex has been studied in detail in human and insect systems, ...
DOI:
10.1111/pbi.13786

3. Anti-TMV activity and effects of three prieurianin-type limonoids from Munronia henryi

Author:
Ying Yan; Dan Wang; Xiong Zhang; Mingyou Peng; Xiaoyan Yan; Yushang Guo; Mengao Jia; Jie Zhou; Lei Tang; Xiaojiang Hao
Source:
Pesticide biochemistry and physiology 2022 v.184 pp. 105108
ISSN:
0048-3575
Subject:
Tobacco mosaic virus,   etc   ; antiviral properties; enzyme activity; limonoids; pathogens; pesticides; physiology; systemic acquired resistance; tobacco;     Show all 9 Subjects
Abstract:
... Three prieurianin-type limonoids (1–3), including two new compounds (1 and 2) and one known compound (3) were isolated from Munronia henryi. These compounds were tested for their activities against tobacco mosaic virus (TMV) by the conventional half-leaf method and the outcomes were analyzed by western blotting and RT-PCR assays. The three tested compounds, at 100 μg/mL, showed strong antiviral ac ...
DOI:
10.1016/j.pestbp.2022.105108

4. High resolution imaging of viruses: Scanning probe microscopy and related techniques

Author:
Assel I. Akhmetova; Igor V. Yaminsky
Source:
Methods 2022 v.197 pp. 30-38
ISSN:
1046-2023
Subject:
Tobacco mosaic virus,   etc   ; adhesion; air; computer software; crystallization; humans; metrology; potatoes; topography; vaccines; vertebrate viruses; viruses;     Show all 12 Subjects
Abstract:
... Scanning probe microscopy is a group of measurements that provides 3D visualization of viruses in different environmental conditions including liquids and air. Besides 3D topography it is possible to measure the properties like mechanical rigidity and stability, adhesion, tendency to crystallization, surface charge, etc. Choosing the right substrate and scanning parameters makes it much easier to ...
DOI:
10.1016/j.ymeth.2021.06.011

5. A Reduced Starch Level in Plants at Early Stages of Infection by Viruses Can Be Considered a Broad-Range Indicator of Virus Presence

Author:
Wanying Zhao; Li Wang; Meizi Liu; Dong Zhang; Ida Bagus Andika; Ying Zhu; Liying Sun
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
Tobacco mosaic virus,   etc   ; Cucumber mosaic virus; DNA; Geminiviridae; Nicotiana benthamiana; Potato virus Y; RNA; apples; biosynthesis; potatoes; starch; transcription (genetics); viruses;     Show all 13 Subjects
Abstract:
... The diagnosis of virus infection can facilitate the effective control of plant viral diseases. To date, serological and molecular methods for the detection of virus infection have been widely used, but these methods have disadvantages if applied for broad-range and large-scale detection. Here, we investigated the effect of infection of several different plant RNA and DNA viruses such as cucumber m ...
DOI:
10.3390/v14061176

6. In vitro biological activities of the flexible and virus nanoparticle-decorated silk fibroin-based films

Author:
Jutathip Panchamanee; Chavee Laomeephol; Jittima Amie Luckanagul; Qian Wang; Siriporn Damrongsakkul
Source:
International journal of biological macromolecules 2022 v.216 pp. 437-445
ISSN:
0141-8130
Subject:
Tobacco mosaic virus,   etc   ; biocompatible materials; cell cycle checkpoints; fibroins; gelatin; glycerol; humans; integrins; silk; surface roughness; viruses; water solubility; water vapor;     Show all 13 Subjects
Abstract:
... Flexible films were prepared from silk fibroin (SF) and gelatin (GA) with a presence of glycerol (Gly), followed by water vapor annealing to achieve water-insoluble matrices. The blended SF/GA/Gly films were chemically conjugated with tobacco mosaic virus (TMV), either native (TMV-wt) or genetically modified with Arg-Gly-Asp (RGD) sequences (TMV-rgd), to improve cellular responses. The attachment ...
DOI:
10.1016/j.ijbiomac.2022.07.011

7. Marine Sesquiterpenes for Plant Protection: Discovery of Laurene Sesquiterpenes and Their Derivatives as Novel Antiviral and Antiphytopathogenic Fungal Agents

Author:
Xin Ding; Yubin Xu; Lili Yan; Lei Chen; Zujia Lu; Caiyan Ge; Xinyi Zhao; Ziwen Wang; Aidang Lu; Qingmin Wang
Source:
Journal of agricultural and food chemistry 2022 v.70 no.20 pp. 6006-6014
ISSN:
1520-5118
Subject:
Tobacco mosaic virus,   etc   ; antifungal properties; antiviral properties; coat proteins; drug resistance; food chemistry; fungi; mechanism of action; pesticides; plant protection; sesquiterpenoids;     Show all 11 Subjects
Abstract:
... The unreasonable use or long-term use of a single variety of pesticide has led to drug resistance and made the pesticides ineffective. Therefore, the creation of new, efficient, and low-risk pesticides is imminent. Marine natural products play a vital role in serving as new lead compounds. In this work, we realized the efficient preparation of nine marine sesquiterpenes with the Stork–Danheiser re ...
DOI:
10.1021/acs.jafc.2c00664

8. Trifluoromethylpyridine thiourea derivatives: design, synthesis and inhibition of the self‐assembly of tobacco mosaic virus particles

Author:
Fangzhou Xu; Shengxin Guo; Wei Zhang; Yanyan Wang; Panpan Wei; Shunhong Chen; Jian Wu
Source:
Pest management science 2022 v.78 no.4 pp. 1417-1427
ISSN:
1526-498X
Subject:
Tobacco mosaic virus,   etc   ; antiviral properties; coat proteins; cross resistance; mechanism of action; median effective concentration; pest management; plant protection; thiourea; viruses;     Show all 10 Subjects
Abstract:
... BACKGROUND: Devastating plant virus diseases leading to bad harvests and lower quality of crops have made feeding the beyond seven billion population a huge challenge. Nevertheless, growing resistance and cross resistance of crop protection agents have made this challenge harder. Therefore, an efficient crop protection agent with novel structure and mode of action showing higher efficiency and eco ...
DOI:
10.1002/ps.6758

9. Cuscuta spp. populations as potential reservoirs and vectors of four plant viruses

Author:
Denitsa Teofanova; Yana Lozanova; Kosara Lambovska; Kalina Pachedjieva; Anita Tosheva; Mariela Odjakova; Lyuben Zagorchev
Source:
Phytoparasitica 2022 v.50 no.3 pp. 555-566
ISSN:
0334-2123
Subject:
Tobacco mosaic virus,   etc   ; Alfalfa mosaic virus; Cucumber mosaic virus; Cuscuta campestris; Tomato yellow leaf curl virus; growth and development; parasitic plants; Bulgaria;     Show all 8 Subjects
Abstract:
... Members of the genus Cuscuta or dodders represent widely distributed holoparasitic flowering plants with large agricultural and ecological significance. Besides their direct negative impact on the growth and development of host plants, they are also regarded as putative vectors of various pathogens, including plant viruses. In the present study, a total of 36 populations, belonging to 4 Cuscuta sp ...
DOI:
10.1007/s12600-022-00981-9

10. Discovery of Phytoalexin Camalexin and Its Derivatives as Novel Antiviral and Antiphytopathogenic-Fungus Agents

Author:
Ancai Liao; Lin Li; Tienan Wang; Aidang Lu; Ziwen Wang; Qingmin Wang
Source:
Journal of agricultural and food chemistry 2022 v.70 no.8 pp. 2554-2563
ISSN:
1520-5118
Subject:
Tobacco mosaic virus,   etc   ; Physalospora; Thanatephorus cucumeris; allelochemicals; antibiotics; antiviral agents; cytosine; fluorescence; food chemistry; hydrogen; mechanism of action; nucleosides; phytoalexins; protective effect; titration; viruses;     Show all 16 Subjects
Abstract:
... In response to the invasion of plant viruses and pathogenic fungi, higher plants produce defensive allelochemicals. Finding candidate varieties of botanical pesticides based on allelochemicals is one of the important ways to create efficient and green pesticides. Here, a series of camalexin derivatives based on a phytoalexin camalexin scaffold were designed, synthesized, and assessed for their ant ...
DOI:
10.1021/acs.jafc.1c07805

11. Physiological and morphological response of tomato plants to nano-chitosan used against bio-stress induced by root-knot nematode (Meloidogyne incognita) and Tobacco mosaic tobamovirus (TMV)

Author:
Mohamed Salah Khalil; Mahmoud Hamdy Abd El-Aziz; Rasha El-Sayed Selim
Source:
European journal of plant pathology 2022 v.163 no.4 pp. 799-812
ISSN:
0929-1873
Subject:
Tobacco mosaic virus,   etc   ; Meloidogyne incognita; catechol oxidase; host range; human health; morphogenesis; nematicides; phenol; plant pathology; protein content; root galls; root-knot nematodes; soil treatment; tomatoes;     Show all 14 Subjects
Abstract:
... Root-knot nematodes (Meloidogyne spp.) have been reported to be responsible for large economic losses of agricultural crops due to their wide host range and variety of suitable climates. The control measures of these parasitic nematodes depend upon synthetic nematicides and a small number bio-based products. Chemical nematicides are eliciting adverse effects on the environment and human health. In ...
DOI:
10.1007/s10658-022-02516-8

12. Evaluation of responses to tomato brown rugose fruit virus (ToBRFV) and selection of resistant lines in Solanum habrochaites and Solanum peruvianum germplasm [Erratum: July 2022, v.88(4); p.285]

Author:
Ahmad Jewehan; Nida Salem; Zoltán Tóth; Pál Salamon; Zoltán Szabó
Source:
Journal of general plant pathology 2022 v.88 no.3 pp. 187-196
ISSN:
1345-2630
Subject:
Tobacco mosaic virus,   etc   ; Solanum habrochaites; Solanum peruvianum; Tomato mosaic virus; alleles; deformation; fruits; germplasm; greenhouses; growth chambers; plant growth; plant pathology; resistance genes; rootstocks; temperature; tomatoes; viruses;     Show all 17 Subjects
Abstract:
... Reactions of plants in 173 wild tomato accessions belonging to Solanum habrochaites and S. peruvianum were studied by inoculation with a tobamovirus, tomato brown rugose fruit virus (ToBRFV). Around 10–50% of plants in nine accessions of S. habrochaites and one of S. peruvianum were demonstrated to be highly resistant. Resistant plants showed no symptoms at 22–24 °C, and no virus could be detected ...
DOI:
10.1007/s10327-022-01055-8

13. The cell‐type specific role of Arabidopsis bZIP59 transcription factor in plant immunity

Author:
Zhiqiang Song; Cheng Zhang; Pinyuan Jin; Charles Tetteh; Xueshuo Dong; Sheng Luo; Siyi Zhang; Xinyuan Li; Yingjun Liu; Huajian Zhang
Source:
Plant, cell and environment 2022 v.45 no.6 pp. 1843-1861
ISSN:
0140-7791
Subject:
Tobacco mosaic virus,   etc   ; Arabidopsis thaliana; Pseudomonas syringae pv. tomato; Sclerotinia sclerotiorum; disease resistance; environment; epistasis; flagellin; immunity; mutants; nitric oxide; reactive oxygen species; salicylic acid; stomatal movement; transcription factors;     Show all 15 Subjects
Abstract:
... Stomatal movement participates in plant immunity by directly affecting the invasion of bacteria, but the genes that regulate stomatal immunity have not been well identified. Here, we characterised the function of the bZIP59 transcription factor from Arabidopsis thaliana, which is constitutively expressed in guard cells. The bzip59 mutant is partially impaired in stomatal closure induced by Pseudom ...
DOI:
10.1111/pce.14299

14. Next-generation sequencing identification and multiplex RT-PCR detection for viruses infecting cigar and flue-cured tobacco

Author:
Tao Zhou; Shidong Zhou; Yong Chen; Jun Wang; Ruina Zhang; Huan Xiang; Zihao Xia; Mengnan An; Xiuxiang Zhao; Yuanhua Wu
Source:
Molecular biology reports 2022 v.49 no.1 pp. 237-247
ISSN:
0301-4851
Subject:
Tobacco mosaic virus,   etc   ; Chilli veinal mottle virus; Cucumber mosaic virus; Potato virus Y; flue-cured tobacco; molecular biology; phylogeny; rapid methods; reverse transcriptase polymerase chain reaction; sequence alignment; viruses; China;     Show all 12 Subjects
Abstract:
... BACKGROUND: Early, precise and simultaneous identification of plant viruses is of great significance for preventing virus spread and reducing losses in agricultural yields. METHODS AND RESULTS: In this study, the identification of plant viruses from symptomatic samples collected from a cigar tobacco planting area in Deyang and a flue-cured tobacco planting area in Luzhou city, Sichuan Province, Ch ...
DOI:
10.1007/s11033-021-06864-2

15. Structural 3D Domain Reconstruction of the RNA Genome from Viruses with Secondary Structure Models

Author:
Simón Poblete; Horacio V. Guzman
Source:
Viruses 2021 v.13 no.8 pp. -
ISSN:
1999-4915
Subject:
Tobacco mosaic virus,   etc   ; RNA; capsid; models; satellites; viral genome;     Show all 6 Subjects
Abstract:
... Three-dimensional RNA domain reconstruction is important for the assembly, disassembly and delivery functionalities of a packed proteinaceus capsid. However, to date, the self-association of RNA molecules is still an open problem. Recent chemical probing reports provide, with high reliability, the secondary structure of diverse RNA ensembles, such as those of viral genomes. Here, we present a meth ...
DOI:
10.3390/v13081555

16. Roles of the EPS66A polysaccharide from Streptomyces sp. in inducing tobacco resistance to tobacco mosaic virus

Author:
Yubo Sun; Hao Wu; Shanshan Xu; Shiqi Tang; Jianjun Hao; Xili Liu; Hongyan Zhang; Lirong Han
Source:
International journal of biological macromolecules 2022 v.209 pp. 885-894
ISSN:
0141-8130
Subject:
Tobacco mosaic virus,   etc   ; Streptomyces; alkalinization; callose; disease resistance; gas chromatography-mass spectrometry; gel chromatography; hypersensitive response; methylation; nitric oxide; nuclear magnetic resonance spectroscopy; pathogens; programmed cell death; salicylic acid; tobacco;     Show all 15 Subjects
Abstract:
... EPS66A was derived from an unidentified Streptomyces sp. HL-66 by chemical fraction and disease-resistance assays. It was identified as a polysaccharide through a series of chemical characterization, including infrared spectrum analysis, methylation, gas chromatography–mass spectrometry, nuclear magnetic resonance, and high-performance gel permeation chromatography. To determine its effect in plan ...
DOI:
10.1016/j.ijbiomac.2022.04.081

17. Establishment of the “Valsa pyri metabolites (VpM)-suspension cell”-based system to study the response of pears to VpM

Author:
Dan Zhao; Yuzhen Tian; Hongqiang Yu; Xia Mao; Chao Wang; Hu Duo; E Sun; Cunwu Zuo
Source:
Physiological and molecular plant pathology 2022 v.120 pp. 101850
ISSN:
0885-5765
Subject:
Tobacco mosaic virus,   etc   ; 2,4-D; Pyrus betulifolia; Pyrus bretschneideri; Valsa; benzyladenine; brassinosteroids; callus; cell proliferation; disease resistance; ethylene; fungi; gene expression regulation; hypersensitive response; industry; metabolites; pears; plant pathology; rootstocks; signal transduction;     Show all 20 Subjects
Abstract:
... Valsa canker, caused by Valsa pyri (Vp), is a necrotrophic fungal disease that seriously damaged the pear industry in the world. Investigations on resistant mechanisms are helpful for resistance breeding and effective control methods. The current study obtained suspension cells of a resistant rootstock ‘Duli-G03’ (Pyrus betulifolia bunge) and a susceptible species ‘Yuluxiang’ (Pyrus bretschneideri ...
DOI:
10.1016/j.pmpp.2022.101850

18. Citral induces plant systemic acquired resistance against tobacco mosaic virus and plant fungal diseases

Author:
Yue Jiang; Xiaolan Ji; Yueyang Zhang; Xiaoyu Pan; Yizhou Yang; Yiming Li; Wenhui Guo; Yong Wang; Zhiqing Ma; Bin Lei; He Yan; Xili Liu
Source:
Industrial crops and products 2022 v.183 pp. 114948
ISSN:
0926-6690
Subject:
Tobacco mosaic virus,   etc   ; Botrytis cinerea; Erysiphe; Litsea cubeba; Sclerotinia sclerotiorum; antiviral properties; bioassays; biosynthesis; chitosan; citral; essential oils; fungi; hypersensitive response; immunity; oils; oligosaccharides; plant disease resistance; salicylic acid; systemic acquired resistance; tobacco;     Show all 20 Subjects
Abstract:
... Litsea cubeba essential oil (LCO) has a wide range of industrial uses. This study found that LCO has significant anti-TMV activity and that its active components are monoterpenoids. Citral, the main active component of the oil, showed outstanding anti-TMV activity. The protective, inactive, and curative efficacies of citral (500 μg/mL) against TMV were 76.27%, 55.14%, and 50.42%, respectively, whi ...
DOI:
10.1016/j.indcrop.2022.114948

19. Synthesis of novel N‐(2‐phenyl‐3‐pyridyl) thiadiazole/isothiazole carboxamide analogs as potent plant elicitors

Author:
Dongyan Yang; Xin Qi; Tatiana A Kalinina; Tatiana V Glukhareva; Liangfu Tang; Zhengming Li; Zhijin Fan
Source:
Pest management science 2022 v.78 no.3 pp. 1138-1145
ISSN:
1526-498X
Subject:
Tobacco mosaic virus,   etc   ; Thanatephorus cucumeris; antifungal properties; bioassays; carboxamides; elicitors; greenhouses; immunity; pathogenesis-related proteins; pest management; plant pathogens; plant protection; quantitative polymerase chain reaction; reactive oxygen species; reverse transcription; salicylic acid; tobacco;     Show all 17 Subjects
Abstract:
... BACKGROUND: Plant elicitors are a class of plant protection agents that can stimulate plant immunity against phytopathogen without a potential resistance problem. In searching for novel plant elicitor candidates, a series of novel N‐(2‐phenyl‐3‐pyridyl) thiadiazole/isothiazole carboxamide analogs were designed and synthesized. RESULTS: In vitro bioassay showed that all new compounds exhibited weak ...
DOI:
10.1002/ps.6728

20. Nicotiana benthamiana asparagine synthetase associates with IP‐L and confers resistance against tobacco mosaic virus via the asparagine‐induced salicylic acid signalling pathway

Author:
Changyun Liu; Shaorui Tian; Xing Lv; Yundan Pu; Haoran Peng; Guangjin Fan; Xiaozhou Ma; Lisong Ma; Xianchao Sun
Source:
Molecular plant pathology 2022 v.23 no.1 pp. 60-77
ISSN:
1464-6722
Subject:
Tobacco mosaic virus,   etc   ; Nicotiana benthamiana; Tomato mosaic virus; ammonium; asparagine; aspartate-ammonia ligase; aspartic acid; cytosol; fluorescence; fluorescent proteins; glutamine; immunity; luciferase; plant pathology; precipitin tests; salicylic acid; tobacco; two hybrid system techniques;     Show all 18 Subjects
Abstract:
... Asparagine synthetase is a key enzyme that catalyses the conversion of amide groups from glutamine or ammonium to aspartate, which leads to the generation of asparagine. However, the role of asparagine synthetase in plant immunity remains largely unknown. Here, we identified a Nicotiana benthamiana asparagine synthetase B (NbAS‐B) that associates with tomato mosaic virus coat protein‐interacting p ...
DOI:
10.1111/mpp.13143