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1. Preparation of DESIGNER extracts of red clover (Trifolium pratense L.) by centrifugal partition chromatography

Author:
Malca Garcia, Gonzalo R.; Friesen, J. Brent; Liu, Yang; Nikolić, Dejan; Lankin, David C.; McAlpine, James B.; Chen, Shao-Nong; Pauli, Guido F.
Source:
Journal of chromatography 2019
ISSN:
0021-9673
Subject:
Trifolium pratense,   etc   ; acetone; acetonitrile; biochanin A; countercurrent chromatography; dimethyl sulfoxide; formononetin; ingredients; kaempferol; solubility; solvents;     Show all 11 Subjects
Abstract:
... Starting with an isoflavone-rich red clover extract (RCE), this study expands on the DESIGNER approach to Deplete and Enrich Select Ingredients to Generate Normalized Extract Resources using countercurrent separation (CCS) methodology. A hydrostatic CCS (also known as centrifugal partition chromatography, CPC) technique was used to enrich and deplete selected bioactive isoflavones of RCE extracts. ...
DOI:
10.1016/j.chroma.2019.05.057

2. Field Evaluation of Potato N Needs Following Forage Legumes

Author:
Kelling, Keith A.; Andraski, Todd W.; Speth, Phillip E.
Source:
American journal of potato research 2019 v.96 no.1 pp. 62-68
ISSN:
1099-209X
Subject:
Trifolium pratense,   etc   ; alfalfa; forage; forage legumes; nitrate nitrogen; nitrogen; nitrogen fertilizers; petioles; potatoes; Wisconsin;     Show all 10 Subjects
Abstract:
... In an attempt to quantify the nitrogen (N) contribution from forage legumes to succeeding potatoes, trials were conducted on 28 Wisconsin alfalfa or red clover fields. Potato total yield and crop value were determined following in-season fertilizer N applications of 0 to 280 kg ha⁻¹. Only 2 of the 12 alfalfa sites and 6 of the 16 red clover sites responded to applied fertilizer N when total yield ...
DOI:
10.1007/s12230-018-9689-2

3. Phylogenetic relationships among introduced and autochthonous rhizobia nodulating Trifolium spp. in Uruguayan soils

Author:
Tartaglia, C.; Azziz, G.; Lorite, M.J.; Sanjuán, J.; Monza, J.
Source:
Applied soil ecology 2019 v.139 pp. 40-46
ISSN:
0929-1393
Subject:
Trifolium pratense,   etc   ; Rhizobium leguminosarum; Trifolium repens; bacteria; farmers; genes; internal transcribed spacers; pastures; phylogeny; ribosomal RNA; soil; symbionts; Uruguay;     Show all 13 Subjects
Abstract:
... Trifolium pratense (red clover) and T. repens (white clover) inoculated with Rhizobium leguminosarum sv. trifolii strain U204 are used to improve pastures in Uruguay. Although inoculation is a common practice in the country, there has been a progressive abandonment by farmers because they do not always see the benefits. The presence of efficient rhizobial populations may be one of the reasons why ...
DOI:
10.1016/j.apsoil.2019.03.014

4. Isoflavone production in hairy root cultures and plantlets of Trifolium pratense

Author:
Reis, Andressa; Boutet-Mercey, Stéphanie; Massot, Sophie; Ratet, Pascal; Zuanazzi, José Angelo Silveira
Source:
Biotechnology letters 2019 v.41 no.3 pp. 427-442
ISSN:
0141-5492
Subject:
Trifolium pratense,   etc   ; biochanin A; daidzein; elicitors; flagellin; formononetin; plantlets; root growth; salicylic acid; seedlings; sucrose; toxicity; yeast extract;     Show all 13 Subjects
Abstract:
... OBJECTIVES: The aim of this study was to develop a Trifolium pratense hairy root (HR) production protocol and select HR lines with high isoflavone yield following elicitor treatments. RESULTS: We obtained 13 independent HR lines, producing approximately three times more isoflavonoids than seedlings (3.3 mg/g dry weight) and in which 27 isoflavonoids were detected. Each HR line had its own isoflavo ...
DOI:
10.1007/s10529-018-02640-8

5. Habitat configuration matters when evaluating habitat‐area effects on host–parasitoid interactions

Author:
With, Kimberly A.; Pavuk, Daniel M.
Source:
Ecosphere 2019 v.10 no.2 pp. e02604
ISSN:
2150-8925
Subject:
Trifolium pratense,   etc   ; Acyrthosiphon pisum; edge effects; habitat destruction; habitat fragmentation; habitats; host-parasitoid relationships; landscapes; models; parasitism; trophic levels;     Show all 11 Subjects
Abstract:
... Higher trophic levels tend to be more sensitive to habitat fragmentation than lower trophic levels, which is why parasitism rates should decline in fragmented landscapes. Habitat loss and fragmentation (the subdivision of habitat) are typically interrelated processes, and thus, their effects are confounded in most studies. To address this, we quantified parasitism rates in pea aphids (Acyrthosipho ...
DOI:
10.1002/ecs2.2604

6. Plant volatile emission depends on the species composition of the neighboring plant community

Author:
Kigathi, Rose N.; Weisser, Wolfgang W.; Reichelt, Michael; Gershenzon, Jonathan; Unsicker, Sybille B.
Source:
BMC plant biology 2019 v.19 no.1 pp. 58
ISSN:
1471-2229
Subject:
Trifolium pratense,   etc   ; Dactylis glomerata; Geranium pratense; Spodoptera littoralis; emissions; greenhouses; host seeking; insect larvae; phytophagous insects; plant communities; plant growth; species richness; volatile organic compounds;     Show all 13 Subjects
Abstract:
... BACKGROUND: Plants grow in multi-species communities rather than monocultures. Yet most studies on the emission of volatile organic compounds (VOCs) from plants in response to insect herbivore feeding focus on one plant species. Whether the presence and identity of neighboring plants or plant community attributes, such as plant species richness and plant species composition, affect the herbivore-i ...
DOI:
10.1186/s12870-018-1541-9

7. Feeding lambs with silage mixtures of grass, sainfoin and red clover improves meat oxidative stability under high oxidative challenge

Author:
Luciano, Giuseppe; Natalello, Antonio; Mattioli, Simona; Pauselli, Mariano; Sebastiani, Bartolomeo; Niderkorn, Vincent; Copani, Giuseppe; Benhissi, Hanen; Amanpour, Asghar; Valenti, Bernardo
Source:
Meat science 2019 v.156 pp. 59-67
ISSN:
0309-1740
Subject:
Trifolium pratense,   etc   ; Onobrychis viciifolia; Phleum pratense; catalysts; cooked foods; grasses; highly unsaturated fatty acids; lambs; meat; muscles; oxidative stability; raw meat; silage; vitamin E;     Show all 14 Subjects
Abstract:
... This study investigated the oxidative stability of meat from lambs fed silages in which timothy grass was totally or partially replaced by sainfoin and/or red clover. Five groups of 8 lambs were fed the following silages: timothy grass (T), mixture (50:50) of timothy with either sainfoin (T-SF) or red clover (T-RC), mixture of timothy, sainfoin and red clover (50:25:25; T-SF-RC), or mixture (50:50 ...
DOI:
10.1016/j.meatsci.2019.05.020

8. Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Author:
Fránová, J.; Sarkisova, T.; Jakešová, H.; Koloniuk, I.
Source:
Plant pathology 2019 v.68 no.7 pp. 1276-1286
ISSN:
0032-0862
Subject:
Trifolium pratense,   etc   ; Cytorhabdovirus; Nicotiana occidentalis; Physalis pubescens; RNA; genome; genomics; high-throughput nucleotide sequencing; host plants; necrosis; nucleotide sequences; phylogeny; sap; virion; viruses;     Show all 15 Subjects
Abstract:
... Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification of part of the cytorhabdoviruses genomes were successfully tested with a number of red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms were selected for further studies. Subsequently, two novel plant rhabdoviruses, tenta ...
DOI:
10.1111/ppa.13065

9. Improving mineral nitrogen control by combining catch crops, fertilisation, and straw management in a clay loam soil

Author:
Arlauskienė, Aušra; Cesevičienė, Jurgita; Velykis, Aleksandras
Source:
Acta agriculturæ Scandinavica 2019 v.69 no.5 pp. 422-431
ISSN:
1651-1913
Subject:
Trifolium pratense,   etc   ; Cambisols; Hordeum vulgare; Sinapis alba subsp. alba; autumn; catch crops; chopping; clay loam soils; nitrogen; nitrogen content; organic matter; plowing; soil minerals; spring; spring barley; straw;     Show all 16 Subjects
Abstract:
... This experiment was conducted on a clay loam Cambisol and set out to determine the effects of combining catch crops, variable fertilisation levels, and straw management on the productivity of a spring barley-catch crop agrosystem, on the enrichment of soil with organic matter and nitrogen (N), and on soil mineral N control. Research was carried out in a spring barley (Hordeum vulgare L.) crop with ...
DOI:
10.1080/09064710.2019.1593498

10. Mgl2 Is a Hypothetical Methyltransferase Involved in Exopolysaccharide Production, Biofilm Formation, and Motility in Rhizobium leguminosarum bv. trifolii

Author:
Marczak, Małgorzata; Żebracki, Kamil; Koper, Piotr; Turska-Szewczuk, Anna; Mazur, Andrzej; Wydrych, Jerzy; Wójcik, Magdalena; Skorupska, Anna
Source:
Molecular plant-microbe interactions 2019 v.32 no.7 pp. 899-911
ISSN:
0894-0282
Subject:
Trifolium pratense,   etc   ; Rhizobium leguminosarum bv. trifolii; biofilm; biosynthesis; exopolysaccharides; gene overexpression; genes; lipopolysaccharides; membrane proteins; methyltransferases; models; molecular weight; mutants; nitrogen fixation; prediction; saprophytes; symbiosis;     Show all 17 Subjects
Abstract:
... In this study, functional characterization of the mgl2 gene located near the Pss-I exopolysaccharide biosynthesis region in Rhizobium leguminosarum bv. trifolii TA1 is described. The hypothetical protein encoded by the mgl2 gene was found to be similar to methyltransferases (MTases). Protein homology and template-based modeling facilitated prediction of the Mgl2 structure, which greatly resembled ...
DOI:
10.1094/MPMI-01-19-0026-R

11. Dynamics of the isoflavone metabolome of traditional preparations of Trifolium pratense L.

Author:
Malca-Garcia, Gonzalo R.; Zagal, Daniel; Graham, James; Nikolić, Dejan; Friesen, J. Brent; Lankin, David C.; Chen, Shao-Nong; Pauli, Guido F.
Source:
Journal of ethnopharmacology 2019 v.238 pp. 111865
ISSN:
0378-8741
Subject:
Trifolium pratense,   etc   ; Oriental traditional medicine; bioactive compounds; biochanin A; cholesterol; flowering; formononetin; glucosides; liquid chromatography; menopause; metabolome; nuclear magnetic resonance spectroscopy; phenolic compounds; tandem mass spectrometry;     Show all 14 Subjects
Abstract:
... The flowering tops of Trifolium pratense L., popularly known as red clover, are used in ethnic Western and Traditional Chinese medicine, in a variety of preparations, including infusions, decoctions and tinctures. Red clover has been reported to be helpful for treatment of menopausal symptoms, premenstrual syndrome, mastalgia, high cholesterol, and other conditions.The aims were to compare the che ...
DOI:
10.1016/j.jep.2019.111865

12. Effects of shredding on silage density and fermentation quality

Author:
Samarasinghe, Milani Bhagya; Larsen, Mogens; Johansen, Marianne; Waldemar, Peter; Weisbjerg, Martin Riis
Source:
Grass and forage science 2019 v.74 no.2 pp. 244-253
ISSN:
0142-5242
Subject:
Trifolium pratense,   etc   ; Lolium perenne; Medicago sativa; alfalfa; ammonia; butyrates; dry matter content; fermentation; forage; lactic acid; pH; shredding; silage; silage making; silos; weight loss; wilting;     Show all 17 Subjects
Abstract:
... The effects of shredding forages on the density and fermentation quality of the resulting silages were studied. Lucerne (Medicago sativa L.), red clover (Trifolium pratense L.), perennial ryegrass (Lolium perenne L.) and a grass–clover mixture were harvested and wilted indoors for 1–2 days. The dry‐matter content of the forages after wilting was 192 g/kg, 192 g/kg, 237 g/kg and 214 g/kg respective ...
DOI:
10.1111/gfs.12424

13. Influence of the development stage of perennial forage crops for the recovery yields of extractable proteins using lactic acid fermentation

Author:
Santamaría-Fernández, M.; Karkov Ytting, N.; Lübeck, M.
Source:
Journal of cleaner production 2019 v.218 pp. 1055-1064
ISSN:
0959-6526
Subject:
Trifolium pratense,   etc   ; animal feeding; biorefining; chicory; feedstocks; forage crops; juices; lactic fermentation; leaf protein concentrate; organic production; plant proteins; protein content;     Show all 12 Subjects
Abstract:
... The extraction of leaf proteins from perennial forage crops within a green biorefinery concept represents a promising approach to face the increasing demand for protein arisen in the organic farming sector to feed monogastric animals. Given the background, the present research aims at assessing the protein extractability from three plant species i.e. chicory, red clover and timothy, at different d ...
DOI:
10.1016/j.jclepro.2019.01.292

14. Vanadium elicitation of Trifolium pratense L. cell culture and possible pathways of produced isoflavones transport across the plasma membrane

Author:
Kubes, Jan; Skalicky, Milan; Tumova, Lenka; Martin, Jan; Hejnak, Vaclav; Martinkova, Jaroslava
Source:
Plant cell reports 2019 v.38 no.5 pp. 657-671
ISSN:
0721-7714
Subject:
Trifolium pratense,   etc   ; ABC transporters; biochanin A; brefeldin A; cell culture; daidzein; elicitors; exudation; formononetin; genistin; metabolism; multiple drug resistance; plasma membrane; secondary metabolites; vanadium;     Show all 15 Subjects
Abstract:
... KEY MESSAGE: Vanadium compounds increased the content and release of distinct isoflavones in a Trifolium pratense suspension culture. Regarding transport-mechanism inhibitors, the process was mostly facilitated by ABC proteins and vesicular transport. The transport of isoflavones and other secondary metabolites is an important part of metabolism within plants and cultures in vitro regarding their ...
DOI:
10.1007/s00299-019-02397-y

15. Characterization of nitrogen transformation dynamics in alfalfa and red clover and their mixture silages

Author:
Dong, Zhihao; Chen, Lei; Li, Junfeng; Yuan, Xianjun; Shao, Tao
Source:
Grassland science 2019 v.65 no.2 pp. 109-115
ISSN:
1744-6961
Subject:
Trifolium pratense,   etc   ; alfalfa; alfalfa protein; alfalfa silage; ammonium nitrogen; fermentation; free amino acids; lactic acid; mixing; nitrogen; pH; peptides; protein degradation; protein depletion; silage making;     Show all 15 Subjects
Abstract:
... The objective of this experiment was to characterize the dynamics of nitrogen (N) transformations in alfalfa and red clover and their mixture during ensiling. Alfalfa, red clover and a mixture of them (1:1 on fresh weight) were ensiled for 3, 5, 7, 14 and 30 days. The fermentation qualities and dynamic change of N components of the three silages were determined. Red clover and mixed silages showed ...
DOI:
10.1111/grs.12230

16. Increasing local biodiversity in urban environments: Community development in semi-natural species-rich forb vegetation

Author:
Bjørn, Mona Chor; Weiner, Jacob; Kollmann, Johannes; Ørgaard, Marian
Source:
Landscape and urban planning 2019 v.184 pp. 23-31
ISSN:
0169-2046
Subject:
Trifolium pratense,   etc   ; Achillea millefolium; aboveground biomass; annuals; biennials; biodiversity; buried seeds; community development; field experimentation; forbs; grasses; growing season; perennials; seedlings; spring; survival rate; urban areas; vegetation types;     Show all 18 Subjects
Abstract:
... There is much interest in supporting biodiversity through eye-catching low maintenance requiring herbaceous vegetation types in urban environments. Community development of forb vegetation is not well understood, and experimental studies investigating the potentials and limitations of establishing such vegetation are needed. We performed a field experiment including three mixtures of 19 forb speci ...
DOI:
10.1016/j.landurbplan.2018.12.010

17. Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility

Author:
Vleugels, Tim; Laere, Katrijn Van; Roldán-Ruiz, Isabel; Cnops, Gerda
Source:
Euphytica 2019 v.215 no.4 pp. 79
ISSN:
0014-2336
Subject:
Trifolium pratense,   etc   ; cultivars; diploidy; forage legumes; genotype; males; meiosis; microsporogenesis; ovules; plant breeding; pollen; pollen germination; seed yield; self-pollination; tetraploidy; viability;     Show all 16 Subjects
Abstract:
... Red clover (Trifolium pratense L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yield and ovule abortion, male meiotic abnormalities, and pollen quality in well-characterized, diploid ...
DOI:
10.1007/s10681-019-2405-6

18. Environmental factors may influence interseeded annual ryegrass and red clover establishment and growth more than soil residual herbicide applications

Author:
Stanton, Victoria L.; Haramoto, Erin R.
Source:
Weed technology 2019 v.33 no.2 pp. 296-302
ISSN:
1550-2740
Subject:
Trifolium pratense,   etc   ; Lolium perenne; Zea mays; atrazine; biomass; corn; cover crops; dimethenamid; environmental factors; herbicide resistance; pesticide application; risk; soil; spring; summer; weed control; weeds; Kentucky;     Show all 18 Subjects
Abstract:
... Integrating multiple weed management (cultural, physical, chemical) strategies is often recommended to combat herbicide resistance. With the increased use of interseeded cover crops, the effects of PRE herbicides on their establishment and growth require study. An investigation was conducted in Lexington, KY, in 2016 through 2018 to assess the extent to which commonly used PRE corn herbicide combi ...
DOI:
10.1017/wet.2019.3

19. Chicory and red clover silage in diets to finishing pigs—influence on performance, time budgets and social interactions

Author:
Presto Åkerfeldt, Magdalena; Nihlstrand, J.; Neil, M.; Lundeheim, N.; Andersson, H. K.; Wallenbeck, A.
Source:
Organic agriculture 2019 v.9 no.1 pp. 127-138
ISSN:
1879-4238
Subject:
Trifolium pratense,   etc   ; chicory; crops; dietary energy sources; feed conversion; finishing; liquids; nutrients; organic production; roughage; silage; social behavior; swine; swine feeding; swine production;     Show all 15 Subjects
Abstract:
... Ley crops can be preserved as silage and can serve as a locally produced feedstuff/enrichment for pigs. It is important to determine the potential for using ley crops grown on-farm in pig production and to evaluate dietary inclusion of different roughage types on pig performance and behaviour. This study examined the influence of access to chicory or red clover silage, in combination with a restri ...
DOI:
10.1007/s13165-018-0216-z

20. The measurement and quantification of generalized gradients of soil fertility relevant to plant community ecology

Author:
Daou, Laurent; Shipley, Bill
Source:
Ecology 2019 v.100 no.1 pp. e02549
ISSN:
0012-9658
Subject:
Trifolium pratense,   etc   ; Arabidopsis thaliana; Festuca rubra; Triticum aestivum; grassland soils; growing season; models; net primary productivity; nitrates; plant communities; structural equation modeling; variance; vegetation; Quebec;     Show all 14 Subjects
Abstract:
... We propose an operational definition of soil “fertility” that is applicable to plant community ecology and develop a method of measuring and quantifying it, using structural equations modeling, that is generalizable to soils in different regions whose fertility has different causes. To do this, we used structural equation modeling (SEM). The measurement submodel predicts the latent “generalized fe ...
DOI:
10.1002/ecy.2549