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- Vazquez, Daniela B., et al. Show all 4 Authors
- Journal of molecular biology 2019 v.431 no.5 pp. 1016-1024
- biotechnology; dimerization; engineering; histidine kinase; hydrogen bonding; temperature
- ... To address the mechanism of thermosensing and its implications for molecular engineering, we previously deconstructed the functional components of the bacterial thermosensor DesK, a histidine kinase with a five-span transmembrane domain that detects temperature changes. The system was first simplified by building a sensor that consists of a single chimerical transmembrane segment that retained ful ...
- Vazquez, Daniela B., et al. Show all 9 Authors
- Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.20 pp. 6353-6358
- cell viability; dimerization; mechanism of action; models; serine
- ... DesK is a bacterial thermosensor protein involved in maintaining membrane fluidity in response to changes in environmental temperature. Most likely, the protein is activated by changes in membrane thickness, but the molecular mechanism of sensing and signaling is still poorly understood. Here we aimed to elucidate the mode of action of DesK by studying the so-called “minimal sensor DesK” (MS-DesK) ...
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