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... The present study investigated the potential probiotic effect of Enterococcus faecalis against a pathogenic microorganism, Vibrio harveyi occurring in the giant freshwater prawn (Macrobrachium rosenbergii). V. harveyi was isolated from the intestine of M. rosenbergii through biochemical tests and PCR-based molecular assays. An in-vitro challenge was conducted by mixing isolated probiotic (5.12 log ...
Vibrioharveyi, etc ; chemistry; emodin; formaldehyde; hydrazides; Show all 5 Subjects
Abstract:
... A series of new N-((4,5,7-trimethoxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)methylene)formohydrazide derivatives was synthesized through the coupling of hydrazide compound and emodin formaldehyde obtained by a four-step procedure starting from emodin. The Vibrio harveyi inhibition activity of the newly prepared compounds was evaluated. Results revealed that all compounds showed different degrees of ...
Vibrioharveyi, etc ; DNA; Sparus aurata; antibiotic resistance; aquaculture; bacteriophages; genomics; latent period; multiple drug resistance; pathogens; phage therapy; virulence; Show all 12 Subjects
Abstract:
... Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in ...
Vibrioharveyi, etc ; bioassays; cell free system; kanamycin; Show all 4 Subjects
Abstract:
... Autoinducer-2 (AI-2) is a cell-to-cell signaling molecule that is commonly detected by an AI-2 bioassay with the Vibrio harveyi reporter strain BB170. However, the assay is affected by certain factors that can lead to inaccurate test results. Thus, the objective of this study was to establish the optimum method for detecting AI-2 by evaluating the effect of culture conditions for BB170, the prepar ...
Vibrioharveyi, etc ; Oplegnathus; Toll-like receptors; bacterial infections; gene expression; gene expression regulation; immune response; industry; spleen; transcriptomics; East Asia; Show all 11 Subjects
Abstract:
... As one of the most valuable maricultured species, spotted knifejaw (Oplegnathus punctatus) has high popularity in eastern Asia. In recent years, diseases caused by Vibrio harveyi have brought huge economic losses in spotted knifejaw industry. To better understand the molecular mechanisms of immune response about V. harveyi resistance in spotted knifejaw, a comparative transcriptome analysis was pe ...
Vibrioharveyi, etc ; fluorescent antibody technique; innate immunity; interleukin-12; interleukin-6; macrophages; mice; mitogen-activated protein kinase; models; pathogens; phosphorylation; secretion; Show all 12 Subjects
Abstract:
... Vibrio harveyi is a zoonotic pathogen that can infect humans through wounds and cause severe inflammatory responses. Previous studies have reported that the Toll like receptors (TLR) mediated MAPK, AKT and NF-κB signaling pathways are involved in innate immune system resistance to pathogen invasion. However, the molecular mechanism of these pathways, as well as their involvement in V. harveyi infe ...
Vibrioharveyi, etc ; Anguilla rostrata; antibiotic resistance; atrophy; body weight; eel; epithelial cells; freshwater; glycogen; hemorrhage; kidneys; liver; necrosis; phenotype; research; thrombosis; virulence; Show all 17 Subjects
Abstract:
... Diseases caused by Vibrio harveyi in marine organisms have been generally reported in aquaculture, but there has been no reported V. harveyi infection in fish cultivated in freshwater. Herein, we isolated 3 strains of V. harveyi from diseased American eels (Anguilla rostrata) cultivated in freshwater and subsequently confirmed the virulence of V. harveyi and the extracellular product (ECP). The th ...
... The β-defensins are important components of the vertebrate innate immune system. While mammalian β-defensins have wide-ranging antibacterial and immunomodulatory activities, those of amphibians remain largely uncharacterised. In this study, β-defensin cDNA was identified from the skin transcriptome of the Chinese spiny frog Quasipaa spinosa. This β-defensin (QS-BD) consists of a signal and a matur ...
... Vibriosis, including the luminescent shrimp disease and acute hepatopancreatic necrosis disease (AHPND), with Vibrio harveyi as one of their causative agents, is a major shrimp disease causing huge economic losses in the Philippines, Thailand, Vietnam and other Southeast Asian countries. It is induced through the formation of biofilm as a result of bacterial cell-to-cell communication or quorum se ...
Vibrioharveyi, etc ; Pseudoalteromonas; agar; bacteria; biological control; coculture; ethyl acetate; gas chromatography-mass spectrometry; mariculture; minimum inhibitory concentration; nucleotide sequences; research; sequence analysis; vibriosis; Indonesia; Show all 15 Subjects
Abstract:
... We isolated Pseudoalteromonas xiamenensis STKMTI.2 from the mangrove sediments of Setokok Island, Indonesia. This study aimed to identify the bacterium, investigate antagonistic properties and minimum inhibitory concentration, and detect the active compounds using gas chromatography–mass spectrometry. The bacterium was identified using a biochemical test and 16S rDNA sequence analysis. Bioactive c ...
Vibrioharveyi, etc ; Cynoglossus semilaevis; adaptive immunity; bacterial infections; biomarkers; cell growth; death; financial economics; fish; fish diseases; gene expression regulation; immune system; industry; mucus; pathogens; Show all 15 Subjects
Abstract:
... The half‐smooth tongue sole, Cynoglossus semilaevis, is an important cultured flatfish species. Vibrio harveyi is a common pathogen to this fish, which may result in great economic loss to C. semilaevis culture industry. piRNAs, a non‐coding RNAs with 26–32 nt, have been regarded as promising biomarkers for cancer diagnosis and fish diseases. Here, we extracted the RNA from mucus of C. semilaevis ...
... White spot syndrome virus (WSSV) is one of the most threatening viral pathogens of shrimp worldwide. VP28, the major capsid protein of WSSV, is reported to play an essential role in the interaction with the host cells. Usually, the diagnostic test of WSSV is performed by one-step PCR, which is neither field-usable nor rapid enough. Therefore, the development of early diagnostic tests is imperative ...
Vibrioharveyi, etc ; Epinephelus fuscoguttatus; Epinephelus lanceolatus; Lates calcarifer; antibodies; blood serum; body weight; farms; field experimentation; formalin; grouper; hybrids; mariculture; marine fish; oral vaccination; survival rate; vaccines; vibriosis; Show all 18 Subjects
Abstract:
... Vibriosis is one of the most common threats to farmed grouper; thus, substantial efforts are underway to control the disease. This study presents an oral vaccination against multiple Vibrio spp. in a marine fish with double booster immunisation. The Vibrio harveyi strain VH1 vaccine candidate was selected from infected groupers Epinephelus sp. in a local farm and was formalin inactivated and combi ...
... Vibrio-related diseases cause significant economic impact on global shrimp production. Although toxin expression is a well-established mechanism of Vibrio-related disease in humans, the role of toxin expression in Vibrio disease in aquaculture generally is not well defined. The current study investigated a sustained, rapid onset, mass mortality at a commercial tiger prawn, Penaeus monodon, hatcher ...
... The purpose of this study was to explore the optimal fermentation technology of Chinese herbal medicine formula-Siwu Decoction and the effects of fermented Siwu Decoction (FSW) on the growth performance, immune response, intestinal microflora and anti microbial ability of Litopenaeus vannamei. Response to surface methodology (RSM) was used to optimize the fermentation process of Siwu Decoction. Th ...
Vibrioharveyi, etc ; Pseudomonas aeruginosa; aquaculture; gold; nanosilver; oligopeptides; pH; silver nitrate; sonication; temperature; topology; Show all 11 Subjects
Abstract:
... A strain of Rhodococcus kroppenstedtii was capable of synthesizing gold and silver nanoparticles (AuNPs and AgNPs). R. kroppenstedtii formed AuNPs in a cell associated manner that could be liberated after sonication. Extracellular AgNPs were produced when cell extracts (lysates obtained after incubation of cells with distilled water) were reacted with silver nitrate. For efficient synthesis of nan ...
Chelsea A. Simpson; Blake D. Petersen; Nicholas W. Haas; Logan J. Geyman; Aimee H. Lee; Ram Podicheti; Robert Pepin; Laura C. Brown; Douglas B. Rusch; Michael P. Manzella; Kai Papenfort; Julia C. van Kessel
Vibrioharveyi, etc ; DNA; Vibrio campbellii; biofilm; bioluminescence; genomics; homoserine; marine ecosystems; phylogeny; quorum sensing; regulon; Show all 11 Subjects
Abstract:
... Vibrio campbellii BB120 (previously classified as Vibrio harveyi) is a fundamental model strain for studying quorum sensing in vibrios. A phylogenetic evaluation of sequenced Vibrio strains in Genbank revealed that BB120 is closely related to the environmental isolate V. campbellii DS40M4. We exploited DS40M4's competence for exogenous DNA uptake to rapidly generate greater than 30 isogenic strain ...
Vibrioharveyi, etc ; light; lighting; nutrient availability; photooxidation; physiology; protein composition; proteomics; seawater; solar radiation; starvation; temperature; Show all 12 Subjects
Abstract:
... A number of Vibrio spp. belong to the well-studied model organisms used to understand the strategies developed by marine bacteria to cope with adverse conditions (starvation, suboptimal temperature, solar radiation, etc.) in their natural environments. Temperature and nutrient availability are considered to be the key factors that influence Vibrio harveyi physiology, morphology, and persistence in ...
... The exploitation of macroalgal biomass could provide novel environment-friendly molecules to prevent bacterial biofilm formation. Crude extracts, or polysaccharides, from the DNA-barcoded macroalgae Chaetomorpha aerea (Chlorophyta), and Agardhiella subulata and Hypnea cornuta (Rhodophyta) collected from the coastal brackish Lake of Ganzirri (Italy) were evaluated for their effects on biofilm forma ...
... To explore the immune function of C-type lectin in shrimp, one recombinant C-type lectin (LvLec) was injected into Litopenaeus vannamei. There were four treatments in the experiment: saline group (as control group), recombinant C-type lectin group (LvLec, 1 mg mL⁻¹), Vibrio harveyi group (V. harveyi, 10⁶ cfu mL⁻¹) and recombinant C-type lectin combined with Vibrio harveyi group (LvLec + V. harveyi ...