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1. FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas

Author:
Song, Tao; Hu, Zhongxu; Liu, Jie; Huang, Wei
Source:
Biochemical and biophysical research communications 2021 v.548 pp. 67-73
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; apoptosis; cell cycle checkpoints; glioma; immunohistochemistry; research; therapeutics;     Show all 7 Subjects
Abstract:
... The expression and roles of FLOT2, especially for its underlying mechanism, in gliomas have been rarely revealed. In this study, upregulations of both FLOT2 and EphA2 in gliomas tissues were validated by immunohistochemistry (IHC) staining and Western blot. FLOT2 silencing notably inhibited the growth and invasion of gliomas cells. Simultaneously, FLOT2 depletion suppressed Akt and NF-κB activitie ...
DOI:
10.1016/j.bbrc.2021.02.062

2. A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins

Author:
Hou, Zhanwu; Han, Xiao; Wang, Zhen; Ghazanfar, Sana; Yang, Jeffy; Liu, Huadong
Source:
Journal of chromatography 2021 v.1165 pp. 122527
ISSN:
1570-0232
Subject:
Western blotting,   etc   ; agarose; alkynes; chromatography; disulfides; mass spectrometry; proteome; silver;     Show all 8 Subjects
Abstract:
... Nascent proteome presents dynamic changes in response to a certain stimulus. Thus, monitoring nascent proteome is critical to uncovering the involved biological mechanism. But the low-abundance of nascent proteome against an overwhelming pre-existing proteome limits its identification and quantification. Herein, we present a novel strategy to enrich nascent proteome from whole cell lysate for furt ...
DOI:
10.1016/j.jchromb.2021.122527

3. 40 years Western blotting: A scientific birthday toast

Author:
Moritz, Christian P.
Source:
Journal of proteomics 2020 v.212 pp. 103575
ISSN:
1874-3919
Subject:
Western blotting,   etc   ; analytical methods; proteins;     Show all 3 Subjects
Abstract:
... The Western blot technique was first published 40 years ago. Since then, it has been mentioned in the titles, abstracts, and keywords of more than 400,000 PubMed-listed publications. This anniversary represents an opportunity to look back to its origins, to address the question as to whether the technique is outdated and heading toward being replaced, and to point out current improvements. Publica ...
DOI:
10.1016/j.jprot.2019.103575

4. Promising proteins detected by Western blot from Echinococcus granulosus protoscoleces for predicting early post-surgical outcomes in CE-affected Tunisian children

Author:
Salah, Eya Ben; Barrera, Coralie; Mosbahi, Sana; Gottstein, Bruno; Siles-Lucas, Mar; Belhassen, Samia; Nouri, Abdellatif; BABBA, Hamouda; Millon, Laurence; Sakly, Wahiba
Source:
Parasites & vectors 2021 v.14 no.1 pp. 180
ISSN:
1756-3305
Subject:
Western blotting,   etc   ; Echinococcus granulosus; antibodies; antigens; biomarkers; echinococcosis; immune response; prediction; probability; protoscoleces; relapse; surgery;     Show all 12 Subjects
Abstract:
... BACKGROUND: Cystic echinococcosis (CE) affects predominantly young patients in highly endemic areas. Improved serological methods are needed for the follow-up of CE cases, especially given the high rates of post-surgical relapse that require detection as soon as possible. METHODS: We designed a study to investigate the value of antigenic proteins extracted from Echinococcus granulosus (E. granulos ...
DOI:
10.1186/s13071-021-04679-5

5. The NF-κB signalling pathway and TM7SF3 contribute to liver fibrosis caused by secreted phospholipase A2 of Clonorchis sinensis

Author:
Wu, Yin-Juan; He, Qing; Shang, Mei; Yin, Ying-Xuan; Li, Ye; Du, Xue; Li, Xue-Rong
Source:
Parasites & vectors 2021 v.14 no.1 pp. 152
ISSN:
1756-3305
Subject:
Western blotting,   etc   ; Clonorchis sinensis; antibodies; liver; liver cirrhosis; mice; phospholipase A2; precipitin tests;     Show all 8 Subjects
Abstract:
... BACKGROUND: The NF-κB signalling pathway has been reported to be related to liver fibrosis, and we investigated whether the NF-κB signalling pathway is involved in liver fibrosis caused by secreted phospholipase A2 of Clonorchis sinensis (CssPLA2). Furthermore, expression of the receptor of CssPLA2 on the cell surface of hepatic stellate cells (HSCs) may greatly contribute to liver fibrosis. METHO ...
DOI:
10.1186/s13071-021-04663-z

6. Changes in mRNA and protein levels of gonadotropin releasing hormone and receptor in ovine thymus, lymph node, spleen, and liver during early pregnancy

Author:
Cao, N.; Cao, L.; Gao, M.; Wang, H.; Zhang, L.; Yang, L.
Source:
Domestic animal endocrinology 2021 v.76 pp. 106607
ISSN:
0739-7240
Subject:
Western blotting,   etc   ; estrous cycle; gonadotropins; immunohistochemistry; liver; lymph nodes; pregnancy; quantitative polymerase chain reaction; sheep; spleen;     Show all 10 Subjects
Abstract:
... There exists maternal immunological modification in maternal immune organs during early pregnancy in mammals. Gonadotropin releasing hormone (GnRH) is widely distributed in vertebrate tissues, including immune organs. However, it is unclear that early pregnancy induces expression of GnRH and GnRH receptor (GnRHR) in ovine immune organs. The objective of this study was to explore the expression of ...
DOI:
10.1016/j.domaniend.2021.106607

7. Effects of rapamycin and OSI-027 on α-SMA in lung tissue of SD rat pups with hyperoxic lung injury

Author:
Liang, Mulin; Dang, Hongxing; Li, Qinghe; Huang, Weiben; Liu, Chengjun
Source:
Biochemical and biophysical research communications 2021 v.556 pp. 39-44
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; air; fibrosis; hyperoxia; intraperitoneal injection; lungs; oxygen; rapamycin; rats; research;     Show all 10 Subjects
Abstract:
... To investigate the effect and significance of mammalian target of rapamycin (mTOR) inhibitors on the expression of α-SMA in lung injury induced by high volume fraction of inspired oxygen (hyperoxygen) in SD rat pups.Seventy-two Sprague-Dawley rat pups (age: 3 weeks) were randomly divided into air + saline, hyperoxia + saline, hyperoxia + OSI-027, and hyperoxia + rapamycin groups. Animal models wer ...
DOI:
10.1016/j.bbrc.2021.02.061

8. Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM

Author:
Ottinger, Christopher A.; Smith, Cheyenne R.; Blazer, Vicki S.; Iwanowicz, Luke R.; Vogelbein, Mary Ann; Kaattari, Stephen
Source:
Fish & shellfish immunology 2021 v.113 pp. 20-23
ISSN:
1050-4648
Subject:
Western blotting,   etc   ; Lepomis macrochirus; Micropterus dolomieu; Micropterus salmoides; fish; immunoglobulin G; mice; monoclonal antibodies; shellfish;     Show all 9 Subjects
Abstract:
... A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis o ...
DOI:
10.1016/j.fsi.2021.03.006

9. The N protein of spring viremia of carp virus promotes the ubiquitination and degradation of viperin_sv1 to escape from the fish innate immunity

Author:
Gao, Yan; Xiang, Yu-Hang; Wang, Fang; Ye, Jing; Lu, Yuan-An; Ashraf, Usama; Liu, Xue-Qin
Source:
Aquaculture 2021 v.538 pp. 736583
ISSN:
0044-8486
Subject:
Western blotting,   etc   ; Carp sprivivirus; antiviral properties; aquaculture; fish; innate immunity; protein content; ubiquitination;     Show all 8 Subjects
Abstract:
... Viperin is known as interferon-stimulating genes (ISGs) and plays broad-spectrum antiviral effects during viral infection. Viperin_sv1, a novel viperin splice variant, has recently been identified from SVCV-infected FHM cells with a stronger antiviral effect than viperin by activating IFN signaling pathway. Meanwhile, SVCV infection can suppress the protein level of viperin_sv1 to escape the host ...
DOI:
10.1016/j.aquaculture.2021.736583

10. Overexpression of STAT4 under hypoxia promotes EMT through miR-200a/STAT4 signal pathway

Author:
Li, Yan; Wang, Juan; Chen, Wenyu; Chen, Xiaoping; Wang, Jianhua
Source:
Life sciences 2021 v.273 pp. 119263
ISSN:
0024-3205
Subject:
Western blotting,   etc   ; bioinformatics; hypoxia; luciferase; metastasis; ovarian neoplasms; protein content; signal transduction; transactivators;     Show all 9 Subjects
Abstract:
... Previous reports have found that STAT4 is involved in the epithelial-mesenchymal transition (EMT), thereby regulating the metastasis and invasion of ovarian cancer. However, the mechanisms underlying remain unclear.We first established hypoxia-induced in vivo and in vitro models. The expression levels of signal transducer and activator of transcription 4 (STAT4), the markers of EMT and microRNA-20 ...
DOI:
10.1016/j.lfs.2021.119263

11. miR-646/TET1 mediated demethylation of IRX1 promoter upregulates HIST2H2BE and promotes the progression of invasive ductal carcinoma

Author:
He, Baoxia; Chen, Jinhua; Song, Wenping; Bai, Yongtao
Source:
Genomics 2021 v.113 no.3 pp. 1469-1481
ISSN:
0888-7543
Subject:
Western blotting,   etc   ; apoptosis; breasts; carcinogenesis; carcinoma; cell proliferation; demethylation; genomics; methylation; promoter regions;     Show all 10 Subjects
Abstract:
... This study aimed to explore role of miR-646 in breast IDC.miR-646, TET1, IRX1, and HIST2H2BE expression was detected by RT-qPCR and/or Western blot analysis. The methylation status of IRX1 promoter region was evaluated by methylation specific PCR. ChIP assay was used to determine the enrichment of TET1 at IRX1 promoter region. Loss- and gain-of functions were performed to determine the roles of mi ...
DOI:
10.1016/j.ygeno.2020.12.044

12. ARID1A and PGR proteins interact in the endometrium and reveal a positive correlation in endometriosis

Author:
Kim, Hong Im; Kim, Tae Hoon; Yoo, Jung-Yoon; Young, Steven L.; Lessey, Bruce A.; Ku, Bon Jeong; Jeong, Jae-Wook
Source:
Biochemical and biophysical research communications 2021 v.550 pp. 151-157
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; endometriosis; endometrium; epithelium; humans; mice; pain; precipitin tests; progesterone; progesterone receptors; research;     Show all 11 Subjects
Abstract:
... Endometriosis is a disorder in which endometrial cells normally limited to the lining of the uterus proliferate outside the uterine cavity and can cause pelvic pain and infertility. ARID1A levels are significantly reduced in the eutopic endometrium from women with endometriosis. Uterine specific Arid1a knock-out mice were infertile due to loss of epithelial progesterone receptor (PGR) signaling. H ...
DOI:
10.1016/j.bbrc.2021.02.144

13. LF-rTMS ameliorates social dysfunction of FMR1−/− mice via modulating Akt/GSK-3β signaling

Author:
Hou, Yilin; Zhao, Jiqian; Yang, Dingding; Xuan, Runkang; Xie, Rougang; Wang, Mengmeng; Mo, Huiming; Liang, Lirong; Wang, Wenting; Wu, Shengxi; Wang, Yazhou; Liu, Xufeng
Source:
Biochemical and biophysical research communications 2021 v.550 pp. 22-29
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; autism; cortex; models; mutants; research; transcranial magnetic stimulation; ultrasonics; vocalization;     Show all 9 Subjects
Abstract:
... Autism spectrum disorders (ASD) are a group of neurological disorders which affect approximately 1% of children around the world. Social dysfunction is one of the two core syndromes of ASD, and still lacks effective treatment. Transcranial magnetic stimulation (TMS) is a noninvasive and safe procedure that uses magnetic fields to modulate neural activity. Whether it were effective in modulating so ...
DOI:
10.1016/j.bbrc.2021.02.086

14. CX3CR1 deficiency aggravates brain white matter injury and affects expression of the CD36/15LO/NR4A1 signal

Author:
Wang, Wenzhu; Wang, Jingbo; Tang, Qing; Zhu, Xiwen; Zhu, Rui; Cui, Dandan; Wei, Chunzhu; Liu, Xinjie; Liu, Xingxing; Ran, Shanshan; Pan, Yuzheng; Yu, Jintao
Source:
Biochemical and biophysical research communications 2021 v.549 pp. 47-53
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; anisotropy; astrocytes; brain; brain damage; males; memory; models; nerve tissue; quantitative analysis; research; tail;     Show all 12 Subjects
Abstract:
... To study the effects of CX3CR1 on white matter injury, neurofunction, recognition, and expression of the CD36/15LO/NR4A1 signal in mice with traumatic brain injury (TBI).CX3CR1ᴳFᴾ/ᴳFᴾ, CX3CR1ᴳFᴾ/⁺ and C57BL/6 male mice were randomly divided into 3 groups. We used a controlled cortical impact (CCI) to establish a TBI model and T2wt MRI to detect the TBI lesion. FA and DTI allowed for quantitative e ...
DOI:
10.1016/j.bbrc.2021.02.053

15. Layilin promotes mitochondrial fission by cyclin-dependent kinase 1 and dynamin-related protein 1 activation in HEK293T cells

Author:
Tsutiya, Atsuhiro; Arito, Mitsumi; Tagashira, Takuma; Sato, Masaaki; Omoteyama, Kazuki; Sato, Toshiyuki; Suematsu, Naoya; Kurokawa, Manae S.; Kato, Tomohiro
Source:
Biochemical and biophysical research communications 2021 v.549 pp. 143-149
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; cyclin-dependent kinase; cyclins; lectins; mitochondria; research; transmembrane proteins;     Show all 7 Subjects
Abstract:
... Functions of layilin, a type 1 transmembrane protein with a C-type lectin motif, remain to be clarified. We here investigated precise intracellular localization of layilin and the location-related functions.We used HEK293T cells to assess the co-localization of layilin with different individual organelle markers by double immunostaining. We then investigated mitochondrial morphology in layilin-kno ...
DOI:
10.1016/j.bbrc.2021.02.091

16. LC3B/p62-mediated mitophagy protects A549 cells from resveratrol-induced apoptosis

Author:
Zheng, Jiahua; Wei, Shaochai; Xiao, Tingting; Li, Guanwu
Source:
Life sciences 2021 v.271 pp. 119139
ISSN:
0024-3205
Subject:
Western blotting,   etc   ; antineoplastic activity; apoptosis; fluorescence; fluorescent antibody technique; lung neoplasms; lysosomes; mitochondria; mitophagy; rapamycin; resveratrol;     Show all 11 Subjects
Abstract:
... Complicated mechanisms in cancer cells have been restricting the medicinal value of resveratrol (Res). The mechanisms by which Res exerts its anti-tumor activity in lung cancer cells have diverged among reports in recent years, whether cells choose to undergo autophagic cell death or apoptosis remains controversial. Yet, whether Res-induced autophagic cell death transforms into apoptosis is still ...
DOI:
10.1016/j.lfs.2021.119139

17. CNR1 may reverse progesterone-resistance of endometrial cancer through the ERK pathway

Author:
Ding, Fei; Qiu, Chunping; Li, Wenzhi; Liu, Zhiming; Kong, Deshui; Ma, Xiaohong; Jiang, Jie
Source:
Biochemical and biophysical research communications 2021 v.548 pp. 148-154
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; Lentivirus; databases; flow cytometry; fluorescent antibody technique; genome; progesterone; research; uterine neoplasms; xenotransplantation;     Show all 10 Subjects
Abstract:
... Endocrine therapy is a promising treatment for endometrial cancer (EC) that preserves fertility, however, progesterone-resistance is currently the major challenges. The Cancer Genome Atlas (TCGA) database analysis showed that CNR1 was closely have a negative correlation with overall survival (OS) and relapse-free survival (RFS) in endometrial cancer. To explore the role of CNR1 in progesterone res ...
DOI:
10.1016/j.bbrc.2021.02.038

18. Effects of transforming growth factor-β1 on odontoblastic differentiation in dental papilla cells is determined by IPO7 expression level

Author:
Zhang, Yue; Zhang, Hao; Yuan, Guohua; Yang, Guobin
Source:
Biochemical and biophysical research communications 2021 v.545 pp. 105-111
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; alizarin; cell lines; mice; mineralization; precipitin tests; research; transfection;     Show all 8 Subjects
Abstract:
... Transforming growth factor β1 (TGF-β1) is one of the broad-spectrum growth-promoting factors that participate in tooth development. The influence of TGF-β1 on the odontoblastic differentiation is still controvercy. Mouse primary dental papilla cells (mDPCs) as well as an immortalized mouse dental papilla cell line (mDPC6Ts) were treated with exogenous TGF-β1 during odontoblastic differentiation. R ...
DOI:
10.1016/j.bbrc.2021.01.076

19. The natural compound rutaecarpine promotes white adipocyte browning through activation of the AMPK-PRDM16 axis

Author:
Liu, Xiaomin; Zhang, Yuwei; Chu, Yi; Zhao, Xuemei; Mao, Liufeng; Zhao, Shiting; Lin, Shaoqiang; Hui, Xiaoyan; Gu, Ping; Xu, Yong; Loomes, Kerry; Tang, Shibing; Nie, Tao; Wu, Donghai
Source:
Biochemical and biophysical research communications 2021 v.545 pp. 189-194
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; drugs; mechanism of action; noninsulin-dependent diabetes mellitus; obesity; research; sequence analysis; white adipocytes;     Show all 8 Subjects
Abstract:
... The prevalence of obesity is increasing globally and is associated with many metabolic disorders, such as type 2 diabetes and cardiovascular diseases. In recent years, a number of studies suggest that promotion of white adipose browning represents a promising strategy to combat obesity and its related metabolic disorders. The aim of this study was to identify compounds that induce adipocyte browni ...
DOI:
10.1016/j.bbrc.2021.01.080

20. Ultrafiltration combing with phospholipid affinity-based isolation for metabolomic profiling of urinary extracellular vesicles

Author:
Lou, Doudou; Wang, Yong; Yang, Qinsi; Hu, Liang; Zhu, Qingfu
Source:
Journal of chromatography 2021 v.1640 pp. 461942
ISSN:
0021-9673
Subject:
Western blotting,   etc   ; biomarkers; chromatography; electron microscopy; metabolites; metabolomics; nucleic acids; phospholipids; ultracentrifugation; ultrafiltration; urine;     Show all 11 Subjects
Abstract:
... Recent years have seen the field of extracellular vesicle (EV) studies burgeoning. This is mainly because EV constituents including nucleic acid, proteins, lipids, and metabolites are promising sources towards disease biomarker discovery. However, EV study remains challenging due to the complexity of biofluids as well as technical limitations during sample preparation. Here, we proposed a simple m ...
DOI:
10.1016/j.chroma.2021.461942