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... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
Westernblotting, etc ; Influenza A virus; Oriental traditional medicine; Trollius chinensis; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses; Show all 16 Subjects
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
Westernblotting, etc ; Celastrus orbiculatus; Oriental traditional medicine; actin; analgesics; animals; cell movement; medicinal plants; metalloproteinases; metastasis; microfilaments; neoplasm cells; protein degradation; regulatory proteins; stomach neoplasms; China; Show all 16 Subjects
Abstract:
... The traditional Chinese medicine herb Celastrus orbiculatus Thunb. is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. Recent years, many studies have reported the significant effects of Celastrus orbiculatus Thunb. extract (COE) on gastric cancer. However, the specific mechanism by which COE regulates gastric canc ...
Westernblotting, etc ; DNA; DNA damage; Sparus aurata; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment; Show all 19 Subjects
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
Westernblotting, etc ; Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; adiponectin receptors; agonists; animal models; diet; fluorescent antibody technique; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity; Show all 21 Subjects
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice. ...
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
Westernblotting, etc ; ethanol; models; phosphorylation; toxicity; Show all 5 Subjects
Abstract:
... The assay presented here was designed to assess the immediate effects of ethanol (EtOH) exposure on intracellular signaling activated by BMPs (Bone Morphogenetic Proteins). Previous reports of the relationship between EtOH exposure and BMP-dependent signaling have primarily assessed the expression of individual BMPs, changes in BMP target genes or effects on the phosphorylation level of key downst ...
Westernblotting, etc ; Oriental traditional medicine; Panax notoginseng; adipocytes; adiposity; blood glucose; blood stasis; brown adipose tissue; computer simulation; drugs; energy; fatty liver; glucose; heat production; high fat diet; histopathology; insulin tolerance test; liver; metabolic diseases; metabolic syndrome; metabolism; obesity; pharmacology; protective effect; quantitative polymerase chain reaction; saponins; stomach; therapeutics; weight loss; white adipose tissue; Show all 30 Subjects
Abstract:
... Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on m ...
Westernblotting, etc ; humans; plasmids; polyethyleneimine; puromycin; ultracentrifugation; Show all 6 Subjects
Abstract:
... The current protocol describes the use of lentiviral particles for the delivery of short hairpin RNAs (shRNAs) to both human embryonic stem cells (hESCs) as well as neural progenitor cells (NPCs) derived from hESCs at high efficiency. Lentiviral particles were generated by co-transfecting HEK293T cells using entry vectors (carrying shRNAs) along with packaging plasmids (pAX and pMD2.G) using the l ...
Westernblotting, etc ; colorectal neoplasms; issues and policy; research; Show all 4 Subjects
Abstract:
... This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). The Editor-in-Chief has decided to retract this paper as the results cannot be relied upon. The authors contacted the editorial office admitting to outsourcing some of the experiments, specifically identifying that the Western Blot images were ...
Westernblotting, etc ; Trichinella; early diagnosis; muscles; parasitology; trichinosis; Show all 6 Subjects
Abstract:
... Serological tests are widely used for the detection of Trichinella spp. infections in animals and humans. Despite some limitations, (such as low sensitivity in the early period after infection) ELISA and western blot testing have demonstrated good performance when excretory/secretory products from muscle larvae are used as antigens in agreement with the International Commission on Trichinellosis. ...
Westernblotting, etc ; bladder; males; phenotype; sensation; urination; urine; Show all 7 Subjects
Abstract:
... To assess the functional role of Hyperpolarization-activated cyclic nucleotide-gated gated channel (HCN) subtypes in the aging bladder phenotype characterized by diminished bladder volume sensation (BVS) with or without the detrusor instability (DI).Expression of HCN subtypes was examined by quantitative RT-PCR and Western blot in aged male Fisher 344 rats (n = 15) and young rats (n = 15). Nocturn ...
Westernblotting, etc ; immunohistochemistry; irradiation; lung neoplasms; mice; research; Show all 6 Subjects
Abstract:
... This study explored the effect of low-dose radiation on the expression of immune checkpoint molecules in lung cancer cells and its mechanism, as well as the antitumour effect of combined low-dose radiation and immune checkpoint inhibitors. Western blot analysis was used to assess the expression of the immune checkpoint molecules CD47, PD-L1, FGL-1 and CD155 in lung cancer cells after radiation. We ...
Westernblotting, etc ; adulthood; adults; dogs; genes; immunohistochemistry; puberty; testes; Show all 8 Subjects
Abstract:
... Sertoli, Leydig, and spermatogonial cells proliferate and differentiate from birth to puberty and then stay stable in adulthood. We hypothesized that expressions of spermatogenesis-associated genes are not enhanced with a mere increase of these cells’ numbers. To accept this postulation, we investigated the abundances of Sertoli cell-specific FSHR and AMH, Leydig cell-specific LHR and INSL3, and s ...
Westernblotting, etc ; adenocarcinoma; bioinformatics; biotechnology; lungs; microRNA; protein content; Show all 7 Subjects
Abstract:
... OBJECTIVE: To seek out the mechanism by which C1QTNF6 mediates lung adenocarcinoma (LUAD). METHODS: Differentially expressed mRNAs and miRNAs in LUAD were analyzed using bioinformatics. In LUAD cells, C1QTNF6 mRNA and miR-184 expression were evaluated with qRT-PCR, and C1QTNF6 protein level was assessed by western blot. Cellular behaviors were assessed by colony formation, CCK-8, Transwell, and wo ...
Westernblotting, etc ; colon; enteritis; histopathology; inflammation; lactoferrin; lipopolysaccharides; mice; Show all 8 Subjects
Abstract:
... Infant intestinal development is immature and, thus, is vulnerable to bacterial and viral infections, which damage intestinal development and even induce acute enteritis. Numerous studies have investigated that lactoferrin (LF) has protective effects on the intestine and may play a role in preventing intestinal inflammation in infants. Lactoferrin is divided into 2 types, namely apo-LF and holo-LF ...
Westernblotting, etc ; colorimetry; detection limit; glyceraldehyde-3-phosphate dehydrogenase; nanoparticles; Show all 5 Subjects
Abstract:
... Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) to solve this challenge. SERS nanotags were used as quantitative labels of proteins, which were composed of gold-s ...
Westernblotting, etc ; amygdala; animal behavior; fearfulness; inflammation; memory; pathophysiology; research; Show all 8 Subjects
Abstract:
... Post-traumatic stress disorder (PTSD) is a pathological fear memory-related disease. The persistence of pathological fearful memories is one of the most characteristic symptoms of PTSD. However, this can be eliminated by intervening in reconsolidation. Inflammation is intimately involved in the pathophysiologic progression of PTSD. Amentoflavone (AF) has anti-inflammatory effects. However, the eff ...
Westernblotting, etc ; gels; heat; molecular weight; polyacrylamide; polyacrylamide gel electrophoresis; Show all 6 Subjects
Abstract:
... Tris-Glycine-SDS is the most commonly used running buffer for SDS-PAGE. Relatively long running times, poor resolution of small molecular weight proteins and excessive heat at higher voltages impede its utility for high throughput downstream applications such as western blot. Here we describe a protocol for gradient-like simultaneous separation of small (<10 kDa) and large (>400 kDa) proteins in a ...