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1. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
Western blotting,   etc   ; DNA; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China;     Show all 14 Subjects
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

2. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Influenza A virus; Oriental traditional medicine; Trollius chinensis; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses;     Show all 16 Subjects
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

3. Celastrus orbiculatus Thunb. extract inhibits EMT and metastasis of gastric cancer by regulating actin cytoskeleton remodeling

Author:
Haibo Wang; YuanYuan Luo; Shiya Ou; Tengyang Ni; Zewen Chu; Xinyi Feng; Xiaojun Dai; Xiaochun Zhang; Yanqing Liu
Source:
Journal of ethnopharmacology 2023 v.301 pp. 115737
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Celastrus orbiculatus; Oriental traditional medicine; actin; analgesics; animals; cell movement; medicinal plants; metalloproteinases; metastasis; microfilaments; neoplasm cells; protein degradation; regulatory proteins; stomach neoplasms; China;     Show all 16 Subjects
Abstract:
... The traditional Chinese medicine herb Celastrus orbiculatus Thunb. is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. Recent years, many studies have reported the significant effects of Celastrus orbiculatus Thunb. extract (COE) on gastric cancer. However, the specific mechanism by which COE regulates gastric canc ...
DOI:
10.1016/j.jep.2022.115737

4. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
Western blotting,   etc   ; DNA; DNA damage; Sparus aurata; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment;     Show all 19 Subjects
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

5. Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221

Author:
Jie Yang; Zi-Xuan Wang; Lei Fang; Tu-Shuai Li; Zhi-Hong Liu; Ying Pan; Ling-Dong Kong
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115688
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; adiponectin receptors; agonists; animal models; diet; fluorescent antibody technique; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity;     Show all 21 Subjects
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
DOI:
10.1016/j.jep.2022.115688

6. Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway

Author:
Yuxin Dai; Qiulu Lu; Peiyi Li; Junyu Zhu; Jiaxin Jiang; Tong Zhao; Yue Hu; Kang Ding; Min Zhao
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115690
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Oriental traditional medicine; blood serum; colon; dextran sulfate; enzyme activity; enzyme-linked immunosorbent assay; fluorescent antibody technique; histopathology; interleukin-18; interleukin-6; liquid chromatography; models; myeloperoxidase; necrosis; neoplasms; protein domains; quantitative polymerase chain reaction; salicylic acid; ubiquitination; ulcerative colitis;     Show all 21 Subjects
Abstract:
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice.  ...
DOI:
10.1016/j.jep.2022.115690

7. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Actaea cimicifuga; Oriental traditional medicine; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids;     Show all 27 Subjects
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

8. Assessment of BMP responses in an in vitro model of acute ethanol toxicity

Author:
Naila Habeeb; Sheyda Najafi; Jeanette C. Perron
Source:
MethodsX 2022 v.9 pp. 101631
ISSN:
2215-0161
Subject:
Western blotting,   etc   ; ethanol; models; phosphorylation; toxicity;     Show all 5 Subjects
Abstract:
... The assay presented here was designed to assess the immediate effects of ethanol (EtOH) exposure on intracellular signaling activated by BMPs (Bone Morphogenetic Proteins). Previous reports of the relationship between EtOH exposure and BMP-dependent signaling have primarily assessed the expression of individual BMPs, changes in BMP target genes or effects on the phosphorylation level of key downst ...
DOI:
10.1016/j.mex.2022.101631

9. Uncovering the effect and mechanism of Panax notoginseng saponins on metabolic syndrome by network pharmacology strategy

Author:
Yisa Wang; Peng Ma; Zijing Wang; Mingxia Sun; Biyu Hou; Tianshu Xu; Wenlan Li; Xiuying Yang; Guanhua Du; Tengfei Ji; Guifen Qiang
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115680
ISSN:
0378-8741
Subject:
Western blotting,   etc   ; Oriental traditional medicine; Panax notoginseng; adipocytes; adiposity; blood glucose; blood stasis; brown adipose tissue; computer simulation; drugs; energy; fatty liver; glucose; heat production; high fat diet; histopathology; insulin tolerance test; liver; metabolic diseases; metabolic syndrome; metabolism; obesity; pharmacology; protective effect; quantitative polymerase chain reaction; saponins; stomach; therapeutics; weight loss; white adipose tissue;     Show all 30 Subjects
Abstract:
... Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on m ...
DOI:
10.1016/j.jep.2022.115680

10. Lentiviral mediated delivery of shrnas to hescs and npcs using low-cost cationic polymer polyethylenimine (pei)

Author:
Sheikh Muhammad Abid; Ansari Suraiya Anjum
Source:
Journal of visualized experiments 2022 no.183 pp. e63953
ISSN:
1940-087X
Subject:
Western blotting,   etc   ; humans; plasmids; polyethyleneimine; puromycin; ultracentrifugation;     Show all 6 Subjects
Abstract:
... The current protocol describes the use of lentiviral particles for the delivery of short hairpin RNAs (shRNAs) to both human embryonic stem cells (hESCs) as well as neural progenitor cells (NPCs) derived from hESCs at high efficiency. Lentiviral particles were generated by co-transfecting HEK293T cells using entry vectors (carrying shRNAs) along with packaging plasmids (pAX and pMD2.G) using the l ...
DOI:
10.3791/63953

11. Retraction notice to “Iron chelator-induced up-regulation of Ndrg1 inhibits proliferation and EMT process by targeting Wnt/β-catenin pathway in colon cancer cells” [Biochem. Biophys. Res. Commun. 506/1 (2018) 114–121]

Author:
Zhiqiang Chen; Jing Sun; Tao Li; Yanfeng Liu; Shang Gao; Xuting Zhi; Minhua Zheng
Source:
Biochemical and biophysical research communications 2022 v.615 pp. 172
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; colorectal neoplasms; issues and policy; research;     Show all 4 Subjects
Abstract:
... This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). The Editor-in-Chief has decided to retract this paper as the results cannot be relied upon. The authors contacted the editorial office admitting to outsourcing some of the experiments, specifically identifying that the Western Blot images were ...
DOI:
10.1016/j.bbrc.2022.05.097

12. Serological testing for Trichinella infection in animals and man: Current status and opportunities for advancements

Author:
María Ángeles Gómez-Morales; Simona Cherchi; Alessandra Ludovisi
Source:
Food and Waterborne Parasitology 2022 v.27 pp. e00165
ISSN:
2405-6766
Subject:
Western blotting,   etc   ; Trichinella; early diagnosis; muscles; parasitology; trichinosis;     Show all 6 Subjects
Abstract:
... Serological tests are widely used for the detection of Trichinella spp. infections in animals and humans. Despite some limitations, (such as low sensitivity in the early period after infection) ELISA and western blot testing have demonstrated good performance when excretory/secretory products from muscle larvae are used as antigens in agreement with the International Commission on Trichinellosis. ...
DOI:
10.1016/j.fawpar.2022.e00165

13. Role of hyperpolarization-activated cyclic nucleotide-gated channels in aging bladder phenotype

Author:
Nishant Singh; Irina Zabbarova; Youko Ikeda; Anthony Kanai; Christopher Chermansky; Naoki Yoshimura; Pradeep Tyagi
Source:
Life sciences 2022 v.289 pp. 120203
ISSN:
0024-3205
Subject:
Western blotting,   etc   ; bladder; males; phenotype; sensation; urination; urine;     Show all 7 Subjects
Abstract:
... To assess the functional role of Hyperpolarization-activated cyclic nucleotide-gated gated channel (HCN) subtypes in the aging bladder phenotype characterized by diminished bladder volume sensation (BVS) with or without the detrusor instability (DI).Expression of HCN subtypes was examined by quantitative RT-PCR and Western blot in aged male Fisher 344 rats (n = 15) and young rats (n = 15). Nocturn ...
DOI:
10.1016/j.lfs.2021.120203

14. The mechanism of low-dose radiation-induced upregulation of immune checkpoint molecule expression in lung cancer cells

Author:
Xinan Wan; Mingxing Fang; Tingting Chen; Huihui Wang; Qin Zhou; Yongming Wei; Lin Zheng; Yu Zhou; Kai Chen
Source:
Biochemical and biophysical research communications 2022 v.608 pp. 102-107
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; immunohistochemistry; irradiation; lung neoplasms; mice; research;     Show all 6 Subjects
Abstract:
... This study explored the effect of low-dose radiation on the expression of immune checkpoint molecules in lung cancer cells and its mechanism, as well as the antitumour effect of combined low-dose radiation and immune checkpoint inhibitors. Western blot analysis was used to assess the expression of the immune checkpoint molecules CD47, PD-L1, FGL-1 and CD155 in lung cancer cells after radiation. We ...
DOI:
10.1016/j.bbrc.2022.03.158

15. Sertoli, Leydig, and Spermatogonial Cells’ Specific Gene and Protein Expressions as Dog Testes Evolve from Immature into Mature States

Author:
Vanmathy R. Kasimanickam; Ramanathan K. Kasimanickam
Source:
Animals 2022 v.12 no.3 pp. -
ISSN:
2076-2615
Subject:
Western blotting,   etc   ; adulthood; adults; dogs; genes; immunohistochemistry; puberty; testes;     Show all 8 Subjects
Abstract:
... Sertoli, Leydig, and spermatogonial cells proliferate and differentiate from birth to puberty and then stay stable in adulthood. We hypothesized that expressions of spermatogenesis-associated genes are not enhanced with a mere increase of these cells’ numbers. To accept this postulation, we investigated the abundances of Sertoli cell-specific FSHR and AMH, Leydig cell-specific LHR and INSL3, and s ...
DOI:
10.3390/ani12030271

16. C1QTNF6 Targeted by MiR-184 Regulates the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells

Author:
Xiao Rao; Yunping Lu
Source:
Molecular biotechnology 2022 v.64 no.11 pp. 1279-1287
ISSN:
1073-6085
Subject:
Western blotting,   etc   ; adenocarcinoma; bioinformatics; biotechnology; lungs; microRNA; protein content;     Show all 7 Subjects
Abstract:
... OBJECTIVE: To seek out the mechanism by which C1QTNF6 mediates lung adenocarcinoma (LUAD). METHODS: Differentially expressed mRNAs and miRNAs in LUAD were analyzed using bioinformatics. In LUAD cells, C1QTNF6 mRNA and miR-184 expression were evaluated with qRT-PCR, and C1QTNF6 protein level was assessed by western blot. Cellular behaviors were assessed by colony formation, CCK-8, Transwell, and wo ...
DOI:
10.1007/s12033-022-00495-z

17. Protective effects of recombinant lactoferrin with different iron saturations on enteritis injury in young mice

Author:
L.L. Fan; Q.Q. Yao; H.M. Wu; F. Wen; J.Q. Wang; H.Y. Li; N. Zheng
Source:
Journal of dairy science 2022 v.105 no.6 pp. 4791-4803
ISSN:
0022-0302
Subject:
Western blotting,   etc   ; colon; enteritis; histopathology; inflammation; lactoferrin; lipopolysaccharides; mice;     Show all 8 Subjects
Abstract:
... Infant intestinal development is immature and, thus, is vulnerable to bacterial and viral infections, which damage intestinal development and even induce acute enteritis. Numerous studies have investigated that lactoferrin (LF) has protective effects on the intestine and may play a role in preventing intestinal inflammation in infants. Lactoferrin is divided into 2 types, namely apo-LF and holo-LF ...
DOI:
10.3168/jds.2021-21428

18. Core-shell SERS nanotags-based western blot

Author:
Bing Liu; Hanyu Tang; Qian Liu; Wenwen Wang; Haitao Li; Shiya Zheng; Fei Sun; Xiangwei Zhao
Source:
Talanta 2023 v.253 pp. 123888
ISSN:
0039-9140
Subject:
Western blotting,   etc   ; colorimetry; detection limit; glyceraldehyde-3-phosphate dehydrogenase; nanoparticles;     Show all 5 Subjects
Abstract:
... Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) to solve this challenge. SERS nanotags were used as quantitative labels of proteins, which were composed of gold-s ...
DOI:
10.1016/j.talanta.2022.123888

19. Amentoflavone impairs the reconsolidated fear memories through inhibition of ERK pathway

Author:
Kuisheng Sun; Ruizhu Xie; Zhengjun Yi; Liqian Yin; Yan Guan; Lujuan Wang; Ying Wang; Xiaoyan Ding; Yaning Luan; Huijie Liu; Yuhan Fan
Source:
Biochemical and biophysical research communications 2022 v.622 pp. 8-14
ISSN:
0006-291X
Subject:
Western blotting,   etc   ; amygdala; animal behavior; fearfulness; inflammation; memory; pathophysiology; research;     Show all 8 Subjects
Abstract:
... Post-traumatic stress disorder (PTSD) is a pathological fear memory-related disease. The persistence of pathological fearful memories is one of the most characteristic symptoms of PTSD. However, this can be eliminated by intervening in reconsolidation. Inflammation is intimately involved in the pathophysiologic progression of PTSD. Amentoflavone (AF) has anti-inflammatory effects. However, the eff ...
DOI:
10.1016/j.bbrc.2022.07.024

20. The gradient-like separation and reduced running time with Tris-Tricine-HEPES buffer for SDS-PAGE

Author:
Daciana Catalina Dumut; Dušan Garić; Amanda Centorame; Danuta Radzioch
Source:
Analytical biochemistry 2022 v.653 pp. 114789
ISSN:
0003-2697
Subject:
Western blotting,   etc   ; gels; heat; molecular weight; polyacrylamide; polyacrylamide gel electrophoresis;     Show all 6 Subjects
Abstract:
... Tris-Glycine-SDS is the most commonly used running buffer for SDS-PAGE. Relatively long running times, poor resolution of small molecular weight proteins and excessive heat at higher voltages impede its utility for high throughput downstream applications such as western blot. Here we describe a protocol for gradient-like simultaneous separation of small (<10 kDa) and large (>400 kDa) proteins in a ...
DOI:
10.1016/j.ab.2022.114789