An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
Westernblotting, etc ; ethanol; models; phosphorylation; toxicity; Show all 5 Subjects
Abstract:
... The assay presented here was designed to assess the immediate effects of ethanol (EtOH) exposure on intracellular signaling activated by BMPs (Bone Morphogenetic Proteins). Previous reports of the relationship between EtOH exposure and BMP-dependent signaling have primarily assessed the expression of individual BMPs, changes in BMP target genes or effects on the phosphorylation level of key downst ...
Westernblotting, etc ; Trichinella; early diagnosis; muscles; parasitology; trichinosis; Show all 6 Subjects
Abstract:
... Serological tests are widely used for the detection of Trichinella spp. infections in animals and humans. Despite some limitations, (such as low sensitivity in the early period after infection) ELISA and western blot testing have demonstrated good performance when excretory/secretory products from muscle larvae are used as antigens in agreement with the International Commission on Trichinellosis. ...
Westernblotting, etc ; adulthood; adults; dogs; genes; immunohistochemistry; puberty; testes; Show all 8 Subjects
Abstract:
... Sertoli, Leydig, and spermatogonial cells proliferate and differentiate from birth to puberty and then stay stable in adulthood. We hypothesized that expressions of spermatogenesis-associated genes are not enhanced with a mere increase of these cells’ numbers. To accept this postulation, we investigated the abundances of Sertoli cell-specific FSHR and AMH, Leydig cell-specific LHR and INSL3, and s ...
Westernblotting, etc ; anesthesia; breathing; functional foods; memory; neonates; neurotoxicity; resveratrol; sevoflurane; Show all 9 Subjects
Abstract:
... Inhalation anesthetics have been shown to cause neurodevelopmental disorders and neurotoxic effects. In this study, we aimed to investigate the effect of resveratrol on the possible neurotoxic effect of sevoflurane and the brain-derived neurotrophic factor (BDNF) pathway in newborn rats. The animals were divided into four groups: control, sevoflurane, sevoflurane+resveratrol 25 mg/kg, and sevoflur ...
Westernblotting, etc ; apoptosis; cell proliferation; ecotoxicology; luciferase; pre-eclampsia; pseudogenes; trophoblast; Show all 8 Subjects
Abstract:
... Long noncoding RNAs (lncRNAs) have been reported as critical modulators in many diseases including preeclampsia. Since the association between lncRNA BNIP3P1 and its cognate gene BNIP3 in preeclampsia has been revealed previously, this study aimed to further explore the function and mechanism of BNIP3P1 in preeclampsia. EdU and TUNEL assays revealed that BNIP3P1 or BNIP3 overexpression inhibited t ...
Westernblotting, etc ; Rhodiola; antioxidants; bioavailability; colon; digesta; flavonoids; gallic acid; permeability; Show all 9 Subjects
Abstract:
... Changes in the phenolic profiles and antioxidant and antiproliferative activities of Rhodiola after simulated in vitro digestion were first assessed in this study. Furthermore, permeability and uptake assays as well as RT-qPCR and western blot analyses were performed in order to explore the bioaccessibility of the digesta and its underlying mechanism. The results reveal that in vitro gastrointesti ...
Westernblotting, etc ; apoptosis; cell lines; cytotoxicity; iodides; lung neoplasms; mice; xenotransplantation; Show all 8 Subjects
Abstract:
... In order to improve the potential of celastrol against non-small-cell lung cancer cells, the privileged structure, thiazolidinedione, was introduced into its C-20 carboxylic group with acetylpiperazine as a linker, and the thiazolidinedione-conjugated compounds 10a–10t were prepared. The target compounds were evaluated for their cytotoxic activities against the A549 cell line, and the results show ...
Westernblotting, etc ; Toxoplasma gondii; automation; diagnostic techniques; pregnancy; seroconversion; serology; therapeutics; France; Show all 9 Subjects
Abstract:
... The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to early detect seroconversions during pregnancy. We conducted a multicentre study to evaluate the diagnos ...
Westernblotting, etc ; carcinogenesis; cell proliferation; endometriosis; endometrium; fluorescent antibody technique; immunochemistry; metastasis; Show all 8 Subjects
Abstract:
... Though endometriosis is benign, however, it shares certain characteristics with cancers, such as the ability to invade and metastasize. Previous studies have demonstrated that S-phase kinase associated protein2 (SKP2) promotes invasion, tumorigenesis, and metastasis. However, its correlation with adenomyosis is unclear. Herein, we aimed to look into SKP2 expression patterns and regulatory effects ...
Westernblotting, etc ; breathing; cell growth; cell proliferation; cyclins; cylindrospermopsin; epithelium; humans; metaphase; Show all 9 Subjects
Abstract:
... Cylindrospermopsin (CYN) is a cyanobacterial toxin that occurs worldwide in aquatic environments. It is known for its delayed effects upon oral uptake in animals and humans. A less well studied route of CYN internalisation is the inhalation of CYN-contaminated aerosols. We analyzed potential effects of different CYN concentrations (1, 2.5 and 5 μmol/l) on cultures of immortalised human bronchial e ...
Westernblotting, etc ; birth weight; blastocyst; embryo transfer; mice; microRNA; morphogenesis; sequence analysis; superovulation; Show all 9 Subjects
Abstract:
... The birthweight after frozen embryo transfer (FET) was significantly higher compared with fresh embryo transfer (fresh ET), while the mechanism remains unclear. In this study, we transferred vitrified-warmed or fresh mice blastocysts into pseudopregnant recipients (n = 11 each group) produced by natural mating to avoid the influence of superovulation. The fetal weight, placental weight, placental ...
Westernblotting, etc ; analgesic effect; analgesics; asymmetry; betamethasone; immunohistochemistry; neuroglia; osteoarthritis; pain; spinal cord; Show all 10 Subjects
Abstract:
... Neuropathic pain in osteoarthritis is one of the reasons why the pain is difficult to treat, and P2X4R plays an important role in neuropathic pain. In addition, BoNT/A has been proven to have analgesic effects on both neuropathic pain and osteoarthritis, but its exact mechanism is still unknown. This study aims to investigate the relationship between the analgesic effect of BoNT/A on osteoarthriti ...
Westernblotting, etc ; carcinogens; humans; microcystin-LR; neoplasm cells; neoplasm progression; phosphorylation; prostatic neoplasms; Show all 8 Subjects
Abstract:
... Microcystin-LR (MC-LR) is an environmental toxin that is synthesized by cyanobacteria and considered a potential human carcinogen. However, the role of MC-LR in prostate cancer progression has not been elucidated. The purpose of this study was to investigate the effect of MC-LR on prostate cancer cell invasion and its underlying mechanisms. Transwell assay was performed, and the result showed that ...
Westernblotting, etc ; chitosan; contact angle; electrophoresis; encapsulation; fibroins; nanofibers; neurons; therapeutics; tissue repair; Show all 10 Subjects
Abstract:
... Neural stem cells (NSCs) transplantation therapy is a promising method for neural tissue regeneration. How to enhance the neuronal differentiation of NSCs has been the most challenging aspect of NSCs application. Herein, the microRNA-222 loaded chitosan nanoparticles (miR-222/CS NPs) were incorporated with silk fibroin (SF) nanofibrous scaffolds to enhance neuronal differentiation of NSCs. The enc ...
Westernblotting, etc ; Toxocara; blood serum; disease diagnosis; enzymes; humans; parasitology; patients; toxocariasis; zoonoses; Show all 10 Subjects
Abstract:
... Toxocarosis is a zoonotic disease caused by migration and subsequent localization of nematode larvae of Toxocara spp. in human organs and tissues, which is manifested with development of various non-specific clinical symptoms. Main diagnostic methods are serological and consists in proving the presence of anti-Toxocara IgG antibodies in patient's sera. In humans, anti-Toxocara IgG has been shown t ...
Marta Rybska; Maria Billert; Marek Skrzypski; Magdalena Kubiak; Magdalena Woźna-Wysocka; Anna Łukomska; Tomasz Nowak; Joanna Błaszczyk-Cichoszewska; Małgorzata Pomorska-Mól; Barbara Wąsowska
Westernblotting, etc ; animal reproduction; endometrium; fluorescent antibody technique; hyperplasia; neuropeptides; progesterone; protein synthesis; pyometra; Show all 9 Subjects
Abstract:
... The most common uterine diseases affecting bitches are cystic endometrial hyperplasia (CEH) and pyometra. The neuropeptide phoenixin (PNX) and its receptor (GPR173) are potential key factors involved in the proliferative and inflammatory regulation of the reproductive system in females. This study aimed to evaluate the expression of PNX and GPR173 by qPCR, western blot and immunofluorescence assay ...
Westernblotting, etc ; Eleutherococcus sessiliflorus; apoptosis; cytotoxicity; ethanol; fluorescence; hepatoma; human cell lines; research; triterpenoids; Show all 10 Subjects
Abstract:
... A rich of 3,4-seco-lupane triterpenoids (3,4-SLT), including chiisanoside (CSS), divaroside (DVS), sessiloside-A1 (SSA), chiisanogenin (CSG), sessiligenin (SSG), were isolated from the ethanol extract of the leaves of Eleutherococcus sessiliflorus (LES). The present study was performed to explore the cytotoxic and anti-tumor effects of the isolated five ones, as well as potential molecular mechani ...
Westernblotting, etc ; antimalarials; droplets; drug therapy; electron microscopy; hepatoma; immunohistochemistry; liver; medicine; protein synthesis; Show all 10 Subjects
Abstract:
... Anti-PD-1 was used to treat for many cancers, but the overall response rate of monoclonal antibodies blocking the inhibitory PD-1/PD-L1 was less than 20%. Lipid droplet (LD) deposition reduced chemotherapy efficacy, but whether LD deposition affects anti-PD-1 treatment and its mechanism remains unclear. Dihydroartemisinin (DHA) was FDA proved antimalarial medicine, but its working mechanism on LD ...
Westernblotting, etc ; computer software; databases; hypoxia; nephrotic syndrome; pharmacology; protein synthesis; research; resveratrol; therapeutics; Show all 10 Subjects
Abstract:
... To investigate the molecular mechanism underlying the therapeutic effect of resveratrol for nephrotic syndrome using various online network pharmacology databases. Genes related to resveratrol and nephrotic syndrome were downloaded from TCMSP, PubChem, and SwissTargetPrediction databases, and Venny2.1.0 was used to obtain the intersected genes. The protein interaction (PPI) network of the intersec ...