U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "Western blotting" Remove constraint Subject: "Western blotting"
Start Over

Search

21,021  Search Results

« Previous | 1 - 20 of 21,021 |
Number of results to display per page

Search Results

1. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
Western blotting,   etc   ; DNA; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China;     Show all 14 Subjects
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

2. Assessment of BMP responses in an in vitro model of acute ethanol toxicity

Author:
Naila Habeeb; Sheyda Najafi; Jeanette C. Perron
Source:
MethodsX 2022 v.9 pp. 101631
ISSN:
2215-0161
Subject:
Western blotting,   etc   ; ethanol; models; phosphorylation; toxicity;     Show all 5 Subjects
Abstract:
... The assay presented here was designed to assess the immediate effects of ethanol (EtOH) exposure on intracellular signaling activated by BMPs (Bone Morphogenetic Proteins). Previous reports of the relationship between EtOH exposure and BMP-dependent signaling have primarily assessed the expression of individual BMPs, changes in BMP target genes or effects on the phosphorylation level of key downst ...
DOI:
10.1016/j.mex.2022.101631

3. Serological testing for Trichinella infection in animals and man: Current status and opportunities for advancements

Author:
María Ángeles Gómez-Morales; Simona Cherchi; Alessandra Ludovisi
Source:
Food and Waterborne Parasitology 2022 v.27 pp. e00165
ISSN:
2405-6766
Subject:
Western blotting,   etc   ; Trichinella; early diagnosis; muscles; parasitology; trichinosis;     Show all 6 Subjects
Abstract:
... Serological tests are widely used for the detection of Trichinella spp. infections in animals and humans. Despite some limitations, (such as low sensitivity in the early period after infection) ELISA and western blot testing have demonstrated good performance when excretory/secretory products from muscle larvae are used as antigens in agreement with the International Commission on Trichinellosis. ...
DOI:
10.1016/j.fawpar.2022.e00165

4. Sertoli, Leydig, and Spermatogonial Cells’ Specific Gene and Protein Expressions as Dog Testes Evolve from Immature into Mature States

Author:
Vanmathy R. Kasimanickam; Ramanathan K. Kasimanickam
Source:
Animals 2022 v.12 no.3 pp. -
ISSN:
2076-2615
Subject:
Western blotting,   etc   ; adulthood; adults; dogs; genes; immunohistochemistry; puberty; testes;     Show all 8 Subjects
Abstract:
... Sertoli, Leydig, and spermatogonial cells proliferate and differentiate from birth to puberty and then stay stable in adulthood. We hypothesized that expressions of spermatogenesis-associated genes are not enhanced with a mere increase of these cells’ numbers. To accept this postulation, we investigated the abundances of Sertoli cell-specific FSHR and AMH, Leydig cell-specific LHR and INSL3, and s ...
DOI:
10.3390/ani12030271

5. Does Resveratrol Prevent Sevoflurane Toxicity in Newborn Rats?

Author:
Ayşenur Sümer Coşkun; Hatice Aslı Bedel; Mustafa Munzuroğlu; Narin Derin; Coşkun Usta
Source:
Journal of medicinal food 2022 v.25 no.5 pp. 557-563
ISSN:
1557-7600
Subject:
Western blotting,   etc   ; anesthesia; breathing; functional foods; memory; neonates; neurotoxicity; resveratrol; sevoflurane;     Show all 9 Subjects
Abstract:
... Inhalation anesthetics have been shown to cause neurodevelopmental disorders and neurotoxic effects. In this study, we aimed to investigate the effect of resveratrol on the possible neurotoxic effect of sevoflurane and the brain-derived neurotrophic factor (BDNF) pathway in newborn rats. The animals were divided into four groups: control, sevoflurane, sevoflurane+resveratrol 25 mg/kg, and sevoflur ...
DOI:
10.1089/jmf.2021.0154

6. Dysregulated pseudogene BNIP3P1 inhibited cell proliferation and promoted cell apoptosis in preeclampsia by acting as a competing endogenous RNA for BNIP3

Author:
Haoyu Zheng; Mengna Shi; Zhenjing Chi; Haidong Wang; Hairong Wang; Dong Xu
Source:
Environmental toxicology 2022 v.37 no.5 pp. 971-982
ISSN:
1520-4081
Subject:
Western blotting,   etc   ; apoptosis; cell proliferation; ecotoxicology; luciferase; pre-eclampsia; pseudogenes; trophoblast;     Show all 8 Subjects
Abstract:
... Long noncoding RNAs (lncRNAs) have been reported as critical modulators in many diseases including preeclampsia. Since the association between lncRNA BNIP3P1 and its cognate gene BNIP3 in preeclampsia has been revealed previously, this study aimed to further explore the function and mechanism of BNIP3P1 in preeclampsia. EdU and TUNEL assays revealed that BNIP3P1 or BNIP3 overexpression inhibited t ...
DOI:
10.1002/tox.23159

7. The effect of in vitro gastrointestinal digestion on the phenolic profiles, bioactivities and bioaccessibility of Rhodiola

Author:
Sheng Zhang; Na Deng; Bisheng Zheng; Tong Li; Rui Hai Liu
Source:
Food & function 2022 v.13 no.10 pp. 5752-5765
ISSN:
2042-650X
Subject:
Western blotting,   etc   ; Rhodiola; antioxidants; bioavailability; colon; digesta; flavonoids; gallic acid; permeability;     Show all 9 Subjects
Abstract:
... Changes in the phenolic profiles and antioxidant and antiproliferative activities of Rhodiola after simulated in vitro digestion were first assessed in this study. Furthermore, permeability and uptake assays as well as RT-qPCR and western blot analyses were performed in order to explore the bioaccessibility of the digesta and its underlying mechanism. The results reveal that in vitro gastrointesti ...
DOI:
10.1039/d2fo00469k

8. Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway

Author:
Xuefeng Fu; Qing Mao; Bing Zhang; Jialun Lv; Kunqi Ping; Peng Zhang; Fengwei Lin; Jiaxing Zhao; Yao Feng; Jincheng Yang; Huiyu Wang; Lei Zhang; Yanhua Mou; Shaojie Wang
Source:
Journal of natural products 2022 v.85 no.4 pp. 1147-1156
ISSN:
1520-6025
Subject:
Western blotting,   etc   ; apoptosis; cell lines; cytotoxicity; iodides; lung neoplasms; mice; xenotransplantation;     Show all 8 Subjects
Abstract:
... In order to improve the potential of celastrol against non-small-cell lung cancer cells, the privileged structure, thiazolidinedione, was introduced into its C-20 carboxylic group with acetylpiperazine as a linker, and the thiazolidinedione-conjugated compounds 10a–10t were prepared. The target compounds were evaluated for their cytotoxic activities against the A549 cell line, and the results show ...
DOI:
10.1021/acs.jnatprod.2c00104

9. Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study

Author:
Valeria Meroni; Francesca Genco; Luigia Scudeller; Marie-Pierre Brenier-Pinchart; Hélène Fricker-Hidalgo; Coralie L’Ollivier; Luc Paris; Hervé Pelloux
Source:
Pathogens 2022 v.11 no.6 pp. -
ISSN:
2076-0817
Subject:
Western blotting,   etc   ; Toxoplasma gondii; automation; diagnostic techniques; pregnancy; seroconversion; serology; therapeutics; France;     Show all 9 Subjects
Abstract:
... The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to early detect seroconversions during pregnancy. We conducted a multicentre study to evaluate the diagnos ...
DOI:
10.3390/pathogens11060665

10. SKP2 regulates ZEB1 expression and stimulates eutopic endometrial stromal cell invasion and proliferation of adenomyosis

Author:
Ying Guo; Jianchao Wang; Chunyan Jia; Ying Liao
Source:
Reproductive Biology 2022 v.22 no.1 pp. 100578
ISSN:
1642-431X
Subject:
Western blotting,   etc   ; carcinogenesis; cell proliferation; endometriosis; endometrium; fluorescent antibody technique; immunochemistry; metastasis;     Show all 8 Subjects
Abstract:
... Though endometriosis is benign, however, it shares certain characteristics with cancers, such as the ability to invade and metastasize. Previous studies have demonstrated that S-phase kinase associated protein2 (SKP2) promotes invasion, tumorigenesis, and metastasis. However, its correlation with adenomyosis is unclear. Herein, we aimed to look into SKP2 expression patterns and regulatory effects ...
DOI:
10.1016/j.repbio.2021.100578

11. The cyanotoxin cylindrospermopsin slows down cell cycle progression and extends metaphase duration in immortalised human airway epithelial cells

Author:
Janita Vennmann; Julia Edelmann; Celine Gudra; Sabine Ziesemer; Jan-Peter Hildebrandt
Source:
Toxicon 2022 v.209 pp. 28-35
ISSN:
0041-0101
Subject:
Western blotting,   etc   ; breathing; cell growth; cell proliferation; cyclins; cylindrospermopsin; epithelium; humans; metaphase;     Show all 9 Subjects
Abstract:
... Cylindrospermopsin (CYN) is a cyanobacterial toxin that occurs worldwide in aquatic environments. It is known for its delayed effects upon oral uptake in animals and humans. A less well studied route of CYN internalisation is the inhalation of CYN-contaminated aerosols. We analyzed potential effects of different CYN concentrations (1, 2.5 and 5 μmol/l) on cultures of immortalised human bronchial e ...
DOI:
10.1016/j.toxicon.2022.01.013

12. Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight

Author:
Yuan Zhu; Yunni Cai; Bo Zheng; Min Ding; Yuting Zhang; Jianjun Zhou
Source:
Reproductive Biology 2022 v.22 no.2 pp. 100646
ISSN:
1642-431X
Subject:
Western blotting,   etc   ; birth weight; blastocyst; embryo transfer; mice; microRNA; morphogenesis; sequence analysis; superovulation;     Show all 9 Subjects
Abstract:
... The birthweight after frozen embryo transfer (FET) was significantly higher compared with fresh embryo transfer (fresh ET), while the mechanism remains unclear. In this study, we transferred vitrified-warmed or fresh mice blastocysts into pseudopregnant recipients (n = 11 each group) produced by natural mating to avoid the influence of superovulation. The fetal weight, placental weight, placental ...
DOI:
10.1016/j.repbio.2022.100646

13. BoNT/A alleviates neuropathic pain in osteoarthritis by down-regulating the expression of P2X4R in spinal microglia

Author:
Hui Yang; Lin Wang; Xiao Chu; Xiaojuan Shi; Xinhe Li; Tieshan Li
Source:
Toxicon 2022 v.206 pp. 55-63
ISSN:
0041-0101
Subject:
Western blotting,   etc   ; analgesic effect; analgesics; asymmetry; betamethasone; immunohistochemistry; neuroglia; osteoarthritis; pain; spinal cord;     Show all 10 Subjects
Abstract:
... Neuropathic pain in osteoarthritis is one of the reasons why the pain is difficult to treat, and P2X4R plays an important role in neuropathic pain. In addition, BoNT/A has been proven to have analgesic effects on both neuropathic pain and osteoarthritis, but its exact mechanism is still unknown. This study aims to investigate the relationship between the analgesic effect of BoNT/A on osteoarthriti ...
DOI:
10.1016/j.toxicon.2021.12.012

14. Microcystin-LR induced microfilament rearrangement and cell invasion by activating ERK/VASP/ezrin pathway in DU145 cells

Author:
Xiaowen Zhang; Xiaoyu Yi; Qiang Zhang; Yaqi Tang; Yongzheng Lu; Baojie Liu; Zhifang Pan; Guohui Wang; Weiguo Feng
Source:
Toxicon 2022 v.210 pp. 148-154
ISSN:
0041-0101
Subject:
Western blotting,   etc   ; carcinogens; humans; microcystin-LR; neoplasm cells; neoplasm progression; phosphorylation; prostatic neoplasms;     Show all 8 Subjects
Abstract:
... Microcystin-LR (MC-LR) is an environmental toxin that is synthesized by cyanobacteria and considered a potential human carcinogen. However, the role of MC-LR in prostate cancer progression has not been elucidated. The purpose of this study was to investigate the effect of MC-LR on prostate cancer cell invasion and its underlying mechanisms. Transwell assay was performed, and the result showed that ...
DOI:
10.1016/j.toxicon.2022.02.023

15. Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells

Author:
Zehao Li; Zhiyuan Meng; Zheng Zhao
Source:
Carbohydrate polymers 2022 v.277 pp. 118791
ISSN:
0144-8617
Subject:
Western blotting,   etc   ; chitosan; contact angle; electrophoresis; encapsulation; fibroins; nanofibers; neurons; therapeutics; tissue repair;     Show all 10 Subjects
Abstract:
... Neural stem cells (NSCs) transplantation therapy is a promising method for neural tissue regeneration. How to enhance the neuronal differentiation of NSCs has been the most challenging aspect of NSCs application. Herein, the microRNA-222 loaded chitosan nanoparticles (miR-222/CS NPs) were incorporated with silk fibroin (SF) nanofibrous scaffolds to enhance neuronal differentiation of NSCs. The enc ...
DOI:
10.1016/j.carbpol.2021.118791

16. Study of IgG avidity and the level of specific IgA antibodies and their significance in the diagnosis of human toxocarosis

Author:
Eleonora Kaneva; Iskra Rainova; Rumen Harizanov; Iskren Kaftandjiev
Source:
Experimental parasitology 2022 v.236-237 pp. 108236
ISSN:
0014-4894
Subject:
Western blotting,   etc   ; Toxocara; blood serum; disease diagnosis; enzymes; humans; parasitology; patients; toxocariasis; zoonoses;     Show all 10 Subjects
Abstract:
... Toxocarosis is a zoonotic disease caused by migration and subsequent localization of nematode larvae of Toxocara spp. in human organs and tissues, which is manifested with development of various non-specific clinical symptoms. Main diagnostic methods are serological and consists in proving the presence of anti-Toxocara IgG antibodies in patient's sera. In humans, anti-Toxocara IgG has been shown t ...
DOI:
10.1016/j.exppara.2022.108236

17. Canine cystic endometrial hyperplasia and pyometra may downregulate neuropeptide phoenixin and GPR173 receptor expression

Author:
Marta Rybska; Maria Billert; Marek Skrzypski; Magdalena Kubiak; Magdalena Woźna-Wysocka; Anna Łukomska; Tomasz Nowak; Joanna Błaszczyk-Cichoszewska; Małgorzata Pomorska-Mól; Barbara Wąsowska
Source:
Animal reproduction science 2022 v.238 pp. 106931
ISSN:
0378-4320
Subject:
Western blotting,   etc   ; animal reproduction; endometrium; fluorescent antibody technique; hyperplasia; neuropeptides; progesterone; protein synthesis; pyometra;     Show all 9 Subjects
Abstract:
... The most common uterine diseases affecting bitches are cystic endometrial hyperplasia (CEH) and pyometra. The neuropeptide phoenixin (PNX) and its receptor (GPR173) are potential key factors involved in the proliferative and inflammatory regulation of the reproductive system in females. This study aimed to evaluate the expression of PNX and GPR173 by qPCR, western blot and immunofluorescence assay ...
DOI:
10.1016/j.anireprosci.2022.106931

18. Cytotoxic and anti-tumor effects of 3,4-seco-lupane triterpenoids from the leaves of Eleutherococcus sessiliflorus against hepatocellular carcinoma

Author:
Haohao Wang; Wenliu Yu; Danfeng Zhang; Yan Zhao; Chen Chen; Hongyan Zhu; Enbo Cai; Zhaowei Yan
Source:
Natural product research 2022 v.36 no.4 pp. 1062-1066
ISSN:
1478-6427
Subject:
Western blotting,   etc   ; Eleutherococcus sessiliflorus; apoptosis; cytotoxicity; ethanol; fluorescence; hepatoma; human cell lines; research; triterpenoids;     Show all 10 Subjects
Abstract:
... A rich of 3,4-seco-lupane triterpenoids (3,4-SLT), including chiisanoside (CSS), divaroside (DVS), sessiloside-A1 (SSA), chiisanogenin (CSG), sessiligenin (SSG), were isolated from the ethanol extract of the leaves of Eleutherococcus sessiliflorus (LES). The present study was performed to explore the cytotoxic and anti-tumor effects of the isolated five ones, as well as potential molecular mechani ...
DOI:
10.1080/14786419.2020.1844698

19. Dihydroartemisinin reduced lipid droplet deposition by YAP1 to promote the anti-PD-1 effect in hepatocellular carcinoma

Author:
Liyuan Hao; Yinglin Guo; Qing Peng; Zhiqin Zhang; Jingmin Ji; Yiwei Liu; Yu Xue; Caige Li; Kangning Zheng; Xinli Shi
Source:
Phytomedicine 2022 v.96 pp. 153913
ISSN:
0944-7113
Subject:
Western blotting,   etc   ; antimalarials; droplets; drug therapy; electron microscopy; hepatoma; immunohistochemistry; liver; medicine; protein synthesis;     Show all 10 Subjects
Abstract:
... Anti-PD-1 was used to treat for many cancers, but the overall response rate of monoclonal antibodies blocking the inhibitory PD-1/PD-L1 was less than 20%. Lipid droplet (LD) deposition reduced chemotherapy efficacy, but whether LD deposition affects anti-PD-1 treatment and its mechanism remains unclear. Dihydroartemisinin (DHA) was FDA proved antimalarial medicine, but its working mechanism on LD ...
DOI:
10.1016/j.phymed.2021.153913

20. Exploring the biomolecular mechanism of resveratrol in the treatment of nephrotic syndrome based on network pharmacology

Author:
Jinshuang Wei; Junlin Zhang; Xiuqi Chen; Jiasen Zou; Junyu Wei; Miaoyue Hu; Shiqun Zhu; Yuanhan Qin; Fengying Lei
Source:
Pharmacological research 2022 v.3 pp. 100114
ISSN:
2667-1425
Subject:
Western blotting,   etc   ; computer software; databases; hypoxia; nephrotic syndrome; pharmacology; protein synthesis; research; resveratrol; therapeutics;     Show all 10 Subjects
Abstract:
... To investigate the molecular mechanism underlying the therapeutic effect of resveratrol for nephrotic syndrome using various online network pharmacology databases. Genes related to resveratrol and nephrotic syndrome were downloaded from TCMSP, PubChem, and SwissTargetPrediction databases, and Venny2.1.0 was used to obtain the intersected genes. The protein interaction (PPI) network of the intersec ...
DOI:
10.1016/j.prmcm.2022.100114