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1. Label-free and ratiometric detection of nuclei acids based on graphene quantum dots utilizing cascade amplification by nicking endonuclease and catalytic G-quadruplex DNAzyme

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Fang, Xin; Hu, Xue-Lian; Li, Zai-Jun;     Show all 5 Authors
Source:
Biosensors & bioelectronics 2016 v.81 pp. 214-220
ISSN:
0956-5663
Subject:
DNA; acids; bioassays; biocatalysis; biosensors; detection limit; fluorescence; graphene; quantum dots; recycling; wavelengths
Abstract:
... Herein, we report a ratiometric fluorescence assay based on graphene quantum dots (GQDs) for the ultrasensitive DNA detection by coupling the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for cascade signal amplifications. With o-phenylenediamine acted as the substrate of G-quadruplex/hemin DNAzyme, whose oxidization product (that is, 2,3-diaminophe ...
DOI:
10.1016/j.bios.2016.02.038

2. Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay

Author:
Wu, Xiu–Ming,   et al.   ; Hu, Xue–Lian; Fang, Xin; Li, Zai–Jun; Wang, Guang–Li;     Show all 5 Authors
Source:
Biosensors & bioelectronics 2016 v.77 pp. 666-672
ISSN:
0956-5663
Subject:
2,4-D; alkaline phosphatase; ascorbic acid; biosensors; bovine serum albumin; detection limit; fluorescence; gold; immunoassays; immunoglobulin G; mice; models; nanoparticles; potassium permanganate
Abstract:
... In this work, a novel strategy for modulating the fluorescence of gold nanoclusters (Au NCs) is developed. The fluorescence of bovine serum albumin (BSA) protected Au NCs is firstly quenched by KMnO4 and then restored by ascorbic acid (AA) due to the deterioration/restoration of the surface structure. Based on which, a novel “switch–on” fluorescent assay for probing the activity of alkaline phosph ...
DOI:
10.1016/j.bios.2015.10.046

3. Targeting insect mitochondrial complex I for plant protection

Author:
Wu, Xiu‐Ming,   et al.   ; Yang, Chang‐Qing; Mao, Ying‐Bo; Wang, Ling‐Jian; Shangguan, Xiao‐Xia; Chen, Xiao‐Ya;     Show all 6 Authors
Source:
Plant biotechnology journal 2016 v.14 no.9 pp. 1925-1935
ISSN:
1467-7644
Subject:
Gossypium hirsutum; Helicoverpa armigera; Hemiptera; NAD (coenzyme); Ostrinia furnacalis; cotton; crops; dehydrogenation; double-stranded RNA; electron transport chain; gene silencing; genes; herbivores; insects; larvae; mitochondria; mortality; plant protection; transcriptome; transgenic plants
Abstract:
... Plant engineered to express double‐stranded RNA (dsRNA) can target the herbivorous insect gene for silencing. Although mounting evidence has emerged to support feasibility of this new pest control technology, field application is slow largely due to lack of potent targets. Here, we show that suppression of the gene encoding NDUFV2, a subunit of mitochondrial complex I that catalyses NADH dehydroge ...
DOI:
10.1111/pbi.12553
PubMed:
26914579
PubMed Central:
PMC5069633

4. Versatile and Amplified Biosensing through Enzymatic Cascade Reaction by Coupling Alkaline Phosphatase in Situ Generation of Photoresponsive Nanozyme

Author:
Wu, Xiu-Ming,   et al.   ; Jin, Lu-Yi; Dong, Yu-Ming; Cao, Gen-Xia; Wang, Guang-Li;     Show all 5 Authors
Source:
Analytical chemistry 2015 v.87 no.20 pp. 10429-10436
ISSN:
1520-6882
Subject:
adults; alkaline phosphatase; biocatalysis; biosensors; blood serum; catechol; colorimetry; detection limit; dissolved oxygen; enzymatic hydrolysis; enzyme-linked immunosorbent assay; humans; immunoglobulin G; irradiation; ligands; mice; nanoparticles; oxidation; signal transduction; titanium; titanium dioxide
Abstract:
... The alkaline phosphatase (ALP) biocatalysis followed by the in situ enzymatic generation of a visible light responsive nanozyme is coupled to elucidate a novel amplification strategy by enzymatic cascade reaction for versatile biosensing. The enzymatic hydrolysis of o-phosphonoxyphenol (OPP) to catechol (CA) by ALP is allowed to coordinate on the surface of TiO₂ nanoparticles (NPs) due to the spec ...
DOI:
10.1021/acs.analchem.5b02728

5. Label-free colorimetric sensor for mercury(II) and DNA on the basis of mercury(II) switched-on the oxidase-mimicking activity of silver nanoclusters

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Jin, Lu-Yi; Dong, Yu-Ming; Li, Zai-Jun;     Show all 5 Authors
Source:
Analytica chimica acta 2015 v.871 pp. 1-8
ISSN:
0003-2670
Subject:
DNA; analytical chemistry; biosensors; biotechnology; bovine serum albumin; catalytic activity; colorimetry; dissolved oxygen; hydrogen peroxide; ions; mercury; nanoparticles; nucleic acid hybridization; oxidants; oxidation; silver; thymine
Abstract:
... In this paper, a novel colorimetric biosensor for Hg2+ and DNA molecules is presented based on Hg2+ stimulated oxidase-like activity of bovine serum albumin protected silver clusters (BSA-Ag NCs). Under mild conditions, Hg2+ activated BSA-Ag NCs to show high catalytic activity toward the oxidation of 3,3′,5, 5′-tetramethylbenzidine (TMB) using ambient dissolved oxygen as an oxidant. The oxidase-li ...
DOI:
10.1016/j.aca.2015.02.027

6. An ultrasensitive and universal photoelectrochemical immunoassay based on enzyme mimetics enhanced signal amplification

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Shu, Jun-Xian; Dong, Yu-Ming; Li, Zai-Jun;     Show all 5 Authors
Source:
Biosensors & bioelectronics 2015 v.66 pp. 283-289
ISSN:
0956-5663
Subject:
absorption; antibodies; antigens; catalytic activity; detection limit; electrodes; goats; hydrogen peroxide; hydroquinone; immunoassays; immunoglobulin G; immunosensors; indium tin oxide; mice; nanoparticles; oxidants; oxidation; quantum dots; silver
Abstract:
... An ultrasensitive photoelectrochemical (PEC) immunoassay based on signal amplification by enzyme mimetics was fabricated for the detection of mouse IgG (as a model protein). The PEC immunosensor was constructed by a layer-by-layer assembly of poly (diallyldimethylammonium chloride) (PDDA), CdS quantum dots (QDs), primary antibody (Ab1, polyclonal goat antimouse IgG), and the antigen (Ag, mouse IgG ...
DOI:
10.1016/j.bios.2014.11.033

7. Intrinsic enzyme mimicking activity of gold nanoclusters upon visible light triggering and its application for colorimetric trypsin detection

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Jin, Lu-Yi; Dong, Yu-Ming; Li, Zai-Jun;     Show all 5 Authors
Source:
Biosensors & bioelectronics 2015 v.64 pp. 523-529
ISSN:
0956-5663
Subject:
biosensors; blood serum; bovine serum albumin; colorimetry; detection limit; gold; hydrogen peroxide; irradiation; nanoparticles; peroxidase; trypsin; urine
Abstract:
... In this research, a novel enzyme mimetics based on the photochemical property of gold nanoclusters was demonstrated. It was found that the bovine serum albumin (BSA) stabilized red or blue emitting gold nanoclusters (Au NCs) exhibited enzyme-like activity under visible light irradiation. The BSA–Au NCs had better stability against stringent conditions compared to natural enzyme. In addition, the p ...
DOI:
10.1016/j.bios.2014.09.071

8. A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Liu, Kang-Li; Shu, Jun-Xian; Gu, Tian-Tian; Dong, Yu-Ming; Li, Zai-Jun;     Show all 7 Authors
Source:
Biosensors & bioelectronics 2015 v.69 pp. 106-112
ISSN:
0956-5663
Subject:
DNA; biosensors; catalase; catalytic activity; detection limit; electrodes; enzyme kinetics; hydrogen peroxide; nanoparticles; nucleic acid hybridization; oxygen; platinum; quantum dots
Abstract:
... Photocathode based on p-type PbS quantum dots (QDs) combing a novel signal amplification strategy utilizing catalase (CAT) mimetics was designed and utilized for sensitive photoelectrochemical (PEC) detection of DNA. The bio-bar-coded Pt nanoparticles (NPs)/G-quadruplex/hemin exhibited high CAT-like activity following the Michaelis–Menten model for decomposing H2O2 to water and oxygen, whose activ ...
DOI:
10.1016/j.bios.2015.02.027

9. Using G-Quadruplex/Hemin To “Switch-On” the Cathodic Photocurrent of p-Type PbS Quantum Dots: Toward a Versatile Platform for Photoelectrochemical Aptasensing

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Shu, Jun-Xian; Dong, Yu-Ming; Zhao, Wei-Wei; Xu, Jing-Juan; Chen, Hong-Yuan;     Show all 7 Authors
Source:
Analytical chemistry 2015 v.87 no.5 pp. 2892-2900
ISSN:
1520-6882
Subject:
DNA; electrodes; electron transfer; hydrogen peroxide; organic matter; oxygen; quantum dots; thrombin
Abstract:
... We present a novel photoelectrochemical (PEC) biosensing platform by taking advantage of the phenomenon that hemin intercalated in G-quadruplex “switched-on” the cathode photocurrent of p-type PbS quantum dots (QDs). Photoinduced electron transfer between PbS QDs and G-quadruplex/hemin(III) complexes with the subsequent catalytic oxygen reduction by the reduced G-quadruplex/hemin(II) led to an obv ...
DOI:
10.1021/ac5043945

10. A new approach to light up the application of semiconductor nanomaterials for photoelectrochemical biosensors: Using self-operating photocathode as a highly selective enzyme sensor

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Liu, Kang-Li; Dong, Yu-Ming; Li, Zai-Jun; Zhang, Chi;     Show all 6 Authors
Source:
Biosensors & bioelectronics 2014 v.62 pp. 66-72
ISSN:
0956-5663
Subject:
ascorbic acid; biocatalysts; biosensors; cysteine; dissolved oxygen; dopamine; electrodes; electrolytes; glucose; glucose oxidase; hydrogen peroxide; oxidation; quantum dots; semiconductors
Abstract:
... Due to the intrinsic hole oxidation reaction occurred on the photoanode surface, currently developed photoelectrochemical biosensors suffer from the interference from coexisting reductive species (acting as electron donor) and a novel design strategy of photoelectrode for photoelectrochemical detection is urgently required. In this paper, a self-operating photocathode based on CdS quantum dots sen ...
DOI:
10.1016/j.bios.2014.06.033

11. The pH-dependent interaction of silver nanoparticles and hydrogen peroxide: A new platform for visual detection of iodide with ultra-sensitivity

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Zhu, Xiao-Ying; Dong, Yu-Ming; Jiao, Huan-Jun; Li, Zai-Jun;     Show all 6 Authors
Source:
Talanta 2013 v.107 pp. 146-153
ISSN:
0039-9140
Subject:
colorimetry; hydrogen peroxide; nanosilver; silver; iodides; anions; pH
Abstract:
... Considering the significance and urgency for the recognition and sensing of anions specifically, especially those of biological relevance, herein, a simple and reliable colorimetric iodide sensor that based on pH-dependent interaction of silver nanoparticles (AgNPs) and H₂O₂ was developed. In acidic medium, AgNPs reacted with H₂O₂ to produce Ag⁺ and powerful oxidizing species. The powerful oxidizi ...
DOI:
10.1016/j.talanta.2012.12.029

12. “Oxidative etching-aggregation” of silver nanoparticles by melamine and electron acceptors: An innovative route toward ultrasensitive and versatile functional colorimetric sensors

Author:
Wu, Xiu-Ming,   et al.   ; Wang, Guang-Li; Zhu, Xiao-Ying; Jiao, Huan-Jun; Dong, Yu-Ming; Li, Zai-Jun;     Show all 6 Authors
Source:
Analytica chimica acta 2012 v.747 pp. 92-98
ISSN:
0003-2670
Subject:
colorimetry; detection limit; hydrogen peroxide; iron; melamine; nanosilver; ozone
Abstract:
... An innovative and versatile functional colorimetric sensor for melamine (MA) and H₂O₂ was developed with simplicity, excellent selectivity and ultrasensitivity. The detection mechanism was based on the “oxidative etching-aggregation” of silver nanoparticles (AgNPs) by the cooperation effect of MA and electron acceptors such as H₂O₂, ozone or Fe(NO₃)₃. The detection limits of this method for MA cou ...
DOI:
10.1016/j.aca.2012.08.019

13. Transcriptional Regulation of Plant Secondary MetabolismF

Author:
Wu, Xiu‐Ming,   et al.   ; Yang, Chang‐Qing; Fang, Xin; Mao, Ying‐Bo; Wang, Ling‐Jian; Chen, Xiao‐Ya;     Show all 6 Authors
Source:
Journal of integrative plant biology 2012 v.54 no.10 pp. 703-712
ISSN:
1672-9072
Subject:
biosynthesis; developmental stages; genes; promoter regions; secondary metabolites; transcription (genetics); transcription factors
Abstract:
... Plant secondary metabolites play critical roles in plant‐environment interactions. They are synthesized in different organs or tissues at particular developmental stages, and in response to various environmental stimuli, both biotic and abiotic. Accordingly, corresponding genes are regulated at the transcriptional level by multiple transcription factors. Several families of transcription factors h ...
DOI:
10.1111/j.1744-7909.2012.01161.x

14. Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum)

Author:
Wu, Xiu-Ming,   et al.   ; Yang, Chang-Qing; Ruan, Ju-Xin; Hu, Wen-Li; Mao, Yin-Bo; Chen, Xiao-Ya; Wang, Ling-Jian;     Show all 7 Authors
Source:
Phytochemistry 2013 v.96 pp. 46-56
ISSN:
0031-9422
Subject:
beta-pinene; beta-phellandrene; beta-caryophyllene; mass spectrometry; Gossypium hirsutum; recombinant proteins; alpha-pinene; gossypol; rapid amplification of cDNA ends; viruses; aldehydes; leaves; pathogens; cultivars; complementary DNA; methyl jasmonate; elicitors; gene silencing; in vitro studies; cotton; antifeedants; biosynthesis; expressed sequence tags; gas chromatography; Verticillium dahliae; phytoalexins
Abstract:
... Cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. However, to date little is known about the biosynthesis of volatile terpenes in this crop. Herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, Gossypium hirsutum cv. CCRI12, were d ...
DOI:
10.1016/j.phytochem.2013.09.009
PubMed:
24074555