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María La O Hechavarría; Yaquelín Puchades Izaguirre; Yosel Pérez Pérez; Gabriela Michavila; Mario A. Casas González; Juana Pérez Pérez; Omelio Carvajal Jaime; Joaquín Montalván Delgado; Andrea Peña Malavera; José Mesa Lopez; Atilio P. Castagnaro; María F. Perera
Xanthomonasalbilineans, etc ; DNA fragmentation; cultivars; disease severity; plant pathology; scald diseases; sugarcane; tropical plants; Cuba; Florida; Show all 10 Subjects
Abstract:
... Leaf scald caused by Xanthomonas albilineans in sugarcane is one of the most important diseases around the world since it causes severe losses in both agricultural and industrial yields. In Cuba, resistance to this disease is considered a major selection criterion within the breeding program. The aim of this work was to detect sugarcane cultivars with stable reaction to X. albilineans by using bot ...
... Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three‐dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bact ...
Sushma Sood; Aliya Momotaz; R. Wayne Davidson; Md S. Islam; Hardev Sandhu; Duli Zhao; Miguel Baltazar; Vanessa S. Gordon; Per McCord; Orlando Arbelo Coto
Xanthomonasalbilineans, etc ; Agricultural Research Service; Puccinia melanocephala; Saccharum; Sporisorium; Sugarcane mosaic virus; cold tolerance; cooperative research; cultivars; females; hybrids; leaf rust; major genes; males; orange rust; sand; scald diseases; sugarcane; Florida; Show all 19 Subjects
Abstract:
... ‘CP 12‐1753’ (Reg. no. CV‐203, PI 698657) sugarcane (complex hybrid of Saccharum spp.) cultivar was developed through a cooperative research between USDA‐ARS, the Florida Sugar Cane League, Inc., and the University of Florida. It was derived from a polycross made at Canal Point, FL, on 8 Dec. 2010 between a female parent CP 08‐2409 and several male parents. CP 12‐1753 had 11 harvests in Stage 4: p ...
Héctor Jorge Suárez; Alberto González Marrero; Yosel Pérez Pérez; Oscar Suárez Benitez; Mario García Guzmán; Ismaray Morales Romero; José Angel Dranguet Isbert
Xanthomonasalbilineans, etc ; Saccharum; cultivars; serodiagnosis; sugarcane; Show all 5 Subjects
Abstract:
... One of the factors that limits the production of sugarcane (Saccharum spp.) is the use of poor quality seed. The objective of the work was to demonstrate the feasibility of using the first ratoon from in vitro propagated plants or cuttings as seed with phytosanitary quality with respect to the presence of Xanthomonas albilineans, the causal agent of foliar scald. Two cultivars (C1051-73 and C90-46 ...
Xanthomonasalbilineans, etc ; adenosine; genes; juices; pathogens; plasmids; pulsed-field gel electrophoresis; quantitative polymerase chain reaction; scald diseases; sugarcane; China; Show all 11 Subjects
Abstract:
... Leaf scald, a bacterial disease caused by Xanthomonas albilineans (Ashby) Dowson, is a major limiting factor for sugarcane production worldwide. Accurate identification and quantification of X. albilineans is a prerequisite for successful management of this disease. A sensitive and robust quantitative PCR (qPCR) assay was developed in this study for detection and quantification of X. albilineans u ...
Xanthomonasalbilineans, etc ; DNA; bacteria; cultivars; detection limit; electrochemistry; field experimentation; hybridization; labor; magnetism; pastures; pathogens; phenotype; sap; scald diseases; standard deviation; sugarcane; xylem; Show all 18 Subjects
Abstract:
... Sugarcane leaf scald caused by the bacterium Xanthomonas albilineans is a major disease of sugarcane worldwide. Whereas erratic symptoms make phenotypic detection challenging, molecular methods require expensive instruments and labour, and longer sample-to-answer times. We report a novel method for detection of X. albilineans DNA in sugarcane xylem sap. The method involves (i) boiling lysis-based ...
... Xylella fastidiosa (Xf) is a plant pathogen causing significant losses in agriculture worldwide. Originating from America, this bacterium caused recent epidemics in southern Europe and is thus considered an emerging pathogen. As the European regulations do not authorize antibiotic treatment in plants, alternative treatments are urgently needed to control the spread of the pathogen and eventually t ...
Benjamín Cervantes-Romero; Paulino Pérez-Rodríguez; Philippe Rott; Apolonio Valdez-Balero; Mónica Osnaya-González; Alejandrina Robledo-Paz; Camilo Hernández-Juárez; José Crossa; Greta H. Rosas-Saito; Hilda V. Silva-Rojas
Xanthomonasalbilineans, etc ; acute course; microscopy; pathogenicity; phylogeny; plant protection; scald diseases; sugarcane; Mexico; Show all 9 Subjects
Abstract:
... Disease outbreaks attributed to leaf scald of sugarcane caused by Xanthomonas albilineans were recently reported in southern Mexico. In this study, the distribution of leaf scald was investigated in the main sugarcane producing-states in Mexico. The disease was found in eight of 14 sugarcane-growing states and in 12 of 31 fields. In contaminated fields, incidence of leaf scald based on symptomatic ...
Natalia Mielnichuk; María I. Bianco; Pablo M. Yaryura; Romina P. Bertani; Laila Toum; Yasmín Daglio; María Antonela Colonnella; Leonardo Lizarraga; Atilio P. Castagnaro; Adrián A. Vojnov
... Xanthomonas albilineans (Xa) and X. sacchari (Xs) are both sugarcane pathogens. Xa is the causal agent of leaf scald disease, but there is limited information about the pathogenicity of Xs. The aim of this work was to study virulence factors of native strains of Xa (Xa32, Xa33, and XaM6) and Xs (Xs14 and Xs15) previously isolated from sugarcane with leaf scald symptoms, to gain insight into the bi ...
Xanthomonasalbilineans, etc ; Xanthomonas vasicola; Xylella fastidiosa; bacteria; beta-Proteobacteria; environmental DNA; horizontal gene transfer; phylogeny; Show all 8 Subjects
Abstract:
... Xylella fastidiosa is a xylem-limited bacterium phylogenetically related to the xanthomonads, with an unusually large and diversified range of plant hosts. To ascertain the origin of its peculiarities, its pan-genome was scanned to identify the genes that are not coherent with its phylogenetic position within the order Xanthomonadales. The results of the analysis revealed that a large fraction of ...
Xanthomonasalbilineans, etc ; Saccharum; amino acid motifs; chromosomes; cultivars; data collection; drought; genes; genome-wide association study; hybrids; infection; phylogeny; salicylic acid; salt stress; sequence analysis; signal transduction; stress response; sugarcane; transcriptome; water stress; Show all 20 Subjects
Abstract:
... To systematically analyze mitogen‐activated protein (MAP) kinase gene families and their expression profiles in sugarcane (Saccharum spp. hybrids; Sh) under diverse biotic and abiotic stresses, we identified 15 ShMAPKs, 6 ShMAPKKs and 16 ShMAPKKKs genes in the sugarcane cultivar R570 genome. These were also confirmed in one S. spontaneum genome and two transcriptome datasets of sugarcane trigged b ...
Xanthomonasalbilineans, etc ; Saccharum spontaneum; cultivars; data collection; gene duplication; leaves; pathogens; phylogeny; scald diseases; sugarcane; transcription factors; transcriptome; tropical plants; Show all 13 Subjects
Abstract:
... The basic helix-loop-helix (bHLH) families that act as transcription factors play a central role in regulating a wide range of physiological, metabolic, and developmental processes in higher organisms. Systematic identification and characterization of bHLH gene families has not yet been performed in sugarcane. In the present study, 37 SsbHLH genes (101 total alleles) were identified in Saccharum s ...
Xanthomonasalbilineans, etc ; Saccharum; composts; cultivars; greenhouses; hot water treatment; microbial contamination; oxidation; plant growth; scald diseases; serodiagnosis; sprinkler irrigation; sugarcane; zeolites; Show all 14 Subjects
Abstract:
... The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants free of Xanthomonas albilineans. A greenhouse was modified and the structure was remodeled, a Sarán sha ...
Xanthomonasalbilineans, etc ; DNA methyltransferase; chromosomes; genetic variation; genomics; homologous recombination; lysogeny; pathogens; plasmids; scald diseases; sugarcane; toxins; virulence; China; Guadeloupe; Show all 15 Subjects
Abstract:
... Leaf scald (caused by Xanthomonas albilineans) is an important bacterial disease affecting sugarcane in most sugarcane growing countries, including China. High genetic diversity exists among strains of X. albilineans from diverse geographic regions. To highlight the genomic features associated with X. albilineans from China, we sequenced the complete genome of a representative strain (Xa-FJ1) of t ...
Xanthomonasalbilineans, etc ; bacteria; genes; leaves; nucleotide sequences; phylogeny; ribosomal RNA; sequence analysis; sugarcane; China; Show all 10 Subjects
Abstract:
... Sugarcane leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is an extremely destructive disease. In 2017, suspected symptoms of sugarcane leaf scald was observed, in Baoshan, Mengding and Jinping, Yunnan Province, China. The bacteria was isolated from the symptomatic stalks and was subsequently identified as the causal agent using Koch’s postulates. BLASTN analysis showed that the amplif ...
Xanthomonasalbilineans, etc ; CRISPR-Cas systems; DNA; RNA; Show all 4 Subjects
Abstract:
... Cas2 protein in the CRISPR‐Cas system functions as a scaffold for the acquisition of foreign DNA fragments, and as a nuclease against DNA and RNA substrates. Crystal structures of Cas2 have shown catalytically inactive conformational states that do not explain the mechanism of Cas2 nuclease activity. Here, we report that Xanthomonas albilineans Cas2 (XaCas2) assumes an inactive conformation in sol ...
... Xanthomonas albilineans (Ashby) Dowson (Xa) has devastating consequences on the vascular system of sugarcane plants and causes severe yield loss in sugarcane. In addition, the incubation period of the pathogen is long, and some infected varieties may remain symptomless for months and even years, leading to the spread of this pathogen over a large area. Therefore, it is important to establish a rel ...
Xanthomonasalbilineans, etc ; ABC transporters; DNA topoisomerase (ATP-hydrolysing); Escherichia coli; cash crops; chlorosis; concatenated DNA; discoloration; genes; genetic databases; leaf blade; pathogenicity; phylogeny; polymerase chain reaction; protocols; pulsed-field gel electrophoresis; scald diseases; sequence analysis; China; French West Indies; Show all 20 Subjects
Abstract:
... In recent years, outbreaks of leaf scald have been reported in two chewing cane clones, ‘Guangdong Huangpi’ and ‘Taoshang Guozhe’, in Zhejiang province, China. From May to July 2019, we collected symptomatic samples from Guangdong Huangpi (eight leaves and three stalks) from four farms in Wenling and Taoshang Guozhe (eight leaves and five stalks) from three farms in Ruian, Zhejiang province. Leave ...
... Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order to better understand the molecular mechanisms involved in the resistance of sugarcane to leaf scald, a comparative proteomic study was performed with ...
Xanthomonasalbilineans, etc ; essential genes; genetic databases; islands; leaves; multilocus sequence typing; pathogens; phylogeny; sugar industry; sugarcane; Caribbean; China; Fiji; Florida; French West Indies; Show all 15 Subjects
Abstract:
... Xanthomonas albilineans is the causal agent of leaf scald, a disease that can cause considerable damage to sugarcane industries. This study analysed the phylogenetic relationship of 14 samples of X. albilineans from China and 13 reference strains retrieved from the GenBank database by multilocus sequence analysis (MLSA). To reach this goal, five housekeeping genes of X. albilineans were amplified ...