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- Zhang, Yuefen, et al. Show all 5 Authors
- Analytical methods 2019 v.11 no.18 pp. 2465-2472
- batch systems; electrophoresis; enzyme kinetics; glutaraldehyde; immobilized enzymes; pepsin; polymers; screening; storage quality; storage temperature; storage time
- ... As pepsin is one of the most important enzymes in the digestive system and is closely related to several diseases, an online system was developed to study the kinetics of pepsin and screen inhibitors from natural products. In the present study, pepsin was immobilized onto an organic polymer monolith with glutaraldehyde as the linker. Both the immobilization and electrophoresis conditions were opti ...