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1. PSA detection using label free graphene FET with coplanar electrodes based microfluidic point of care diagnostic device

Author:
Mandal, Naresh; Pakira, Victor; Samanta, Nirmalya; Das, Naren; Chakraborty, Suman; Pramanick, Bidhan; RoyChaudhuri, Chirasree
Source:
Talanta 2021 v.222 pp. 121581
ISSN:
0039-9140
Subject:
bovine serum albumin,   etc   ; biomarkers; blood serum; detection limit; diagnostic techniques; dielectrophoresis; electrodes; electronics; graphene; humans; immunoglobulin G; neoplasms; point-of-care systems; prostate-specific antigen; transistors;     Show all 15 Subjects
Abstract:
... Affordable point-of-care (PoC) diagnostic devices enable detection of prostate specific antigen (PSA) in resource limited settings. Despite the advancements in PoC systems, most of the reported methods for PSA detection have unsatisfactory detection limits and are based on labelled assays, requiring multiple reagent flow steps which increases both expenses and inconvenience. Circumventing these co ...
DOI:
10.1016/j.talanta.2020.121581

2. Growth and albumin secretion of mouse fetal liver cells cryopreserved within porous polymer scaffolds as a viable cell source for bioartificial livers

Author:
Miyoshi, Hirotoshi; Iwamoto, Ayako; Koyama, Toshie
Source:
Journal of bioscience and bioengineering 2020 v.130 no.2 pp. 212-216
ISSN:
1389-1723
Subject:
albumins,   etc   ; cell viability; cryopreservation; hepatocytes; liver; mice; polymers; secretion; thawing;     Show all 9 Subjects
Abstract:
... To clinically apply bioartificial livers (BALs), an effective liver cell cryopreservation method is required for a stable cell supply. In this study, we performed tissue-engineered construct (TEC) cryopreservation of fetal liver cells (FLCs) in which FLCs cultured within a porous polymer scaffold were cryopreserved. Growth and albumin secretion in TEC-cryopreserved FLCs after thawing were compared ...
DOI:
10.1016/j.jbiosc.2020.03.013

3. Molecular insight on the binding of monascin to bovine serum albumin (BSA) and its effect on antioxidant characteristics of monascin

Author:
Wu, Shufen; Wang, Xiaochan; Bao, Yuxin; Zhang, Chuanying; Liu, Huanhuan; Li, Zhenjing; Chen, Mianhua; Wang, Changlu; Guo, Qingbin; Peng, Xin
Source:
Food chemistry 2020 v.315 pp. 126228
ISSN:
0308-8146
Subject:
bovine serum albumin,   etc   ; antioxidant activity; antioxidants; fluorescence; hydrogen bonding; hydrophobicity;     Show all 6 Subjects
Abstract:
... Monascin (MS) is a yellow lipid-soluble azaphilonoid pigment identified from Monascus-fermented products with promising biological activities. This work studied interactions between MS and bovine serum albumin (BSA) as well as their influences on the antioxidant activity of MS. Experimental results demonstrated that the fluorescence emission of BSA was quenched by MS via static quenching mechanism ...
DOI:
10.1016/j.foodchem.2020.126228

4. How the dynamics of subsurface hydration regulates protein-surface interactions

Author:
Bülbül, Ezgi; Hegemann, Dirk; Geue, Thomas; Heuberger, Manfred
Source:
Colloids and surfaces 2020 v.190 pp. 110908
ISSN:
0927-7765
Subject:
bovine serum albumin,   etc   ; adsorbents; adsorption; blood plasma; films (materials); polymers;     Show all 6 Subjects
Abstract:
... The role of water structure near surfaces has been scrutinized extensively because it is accepted to control protein-surface interactions, however, often avoiding effects of hydration dynamics. Relating to this, we have recently discussed how the amount and state of water, accumulated within various hydrophobic-to-hydrophilic subsurface gradients of plasma polymer films, influence the magnitude of ...
DOI:
10.1016/j.colsurfb.2020.110908

5. Investigation of the relationship between ionised and total calcium in dogs with ionised hypercalcaemia

Author:
Tørnqvist‐Johnsen, C.; Schnabel, T.; Gow, A. G.; Del‐Pozo, J.; Milne, E.; Macklin, V.; Fisher, C.; Handel, I.; Mellanby, R. J.
Source:
Thejournal of small animal practice 2020 v.61 no.4 pp. 247-252
ISSN:
0022-4510
Subject:
serum albumin,   etc   ; adults; calcium; databases; dogs; hospitals; hypercalcemia; hypoalbuminemia; ionization;     Show all 9 Subjects
Abstract:
... OBJECTIVES: To investigate the relationship between total and ionised calcium concentrations in dogs with ionised hypercalcaemia and to evaluate how albumin influences this relationship. METHODS: Initially, a reference interval for ionised and total calcium was established using a large population of healthy adult dogs. Our teaching hospital clinical database was searched to identify adult dogs wi ...
DOI:
10.1111/jsap.13109

6. Production of protein-loaded starch microspheres using water-in-water emulsion method

Author:
Yang, Hui; Yang, Yaliao; Li, Bing-Zheng; Adhikari, Benu; Wang, Yong; Huang, Hua-Lin; Chen, Dong
Source:
Carbohydrate polymers 2020 v.231 pp. 115692
ISSN:
0144-8617
Subject:
bovine serum albumin,   etc   ; emulsions; encapsulation; microparticles; molecular weight; solutes; starch;     Show all 7 Subjects
Abstract:
... Protein-loaded starch microspheres were prepared by water-in-water (w/w) emulsion method. The effects of the molecular weight of starch and protein used, concentration of solutes in both dispersed and continuous phases and starch to protein mass ratio on the yield, loading capacity and encapsulation efficiency were measured. These parameters were significantly higher in Bovine serum albumin (BSA)- ...
DOI:
10.1016/j.carbpol.2019.115692

7. Quantum dots mediated fluorescent “turn-off-on” sensor for highly sensitive and selective sensing of protein

Author:
Wang, Xiaodan; Liu, Zhengqing; Gao, Pengfei; Li, Yanjie; Qu, Xiaoyan
Source:
Colloids and surfaces 2020 v.185 pp. 110599
ISSN:
0927-7765
Subject:
human serum albumin,   etc   ; cadmium; detection limit; fluorescence; quantum dots; resveratrol;     Show all 6 Subjects
Abstract:
... We described a highly sensitive and selective strategy for sensing of human serum albumin (HSA) by constructing a reversible fluorescent “turn-off-on” sensor. Monodispersed cadmium telluride quantum dots (CdTe QDs) were synthesized by using a simple aqueous phase synthesis method, which exhibited strong green fluorescence. The CdTe QDs fluorescence was first quenched using resveratrol through dyna ...
DOI:
10.1016/j.colsurfb.2019.110599

8. A chemiresistive biosensor based on a layered graphene oxide/graphene composite for the sensitive and selective detection of circulating miRNA-21

Author:
Huang, Chi-Hsien; Huang, Tzu-Ting; Chiang, Chia-Heng; Huang, Wei-Ting; Lin, Yi-Ting
Source:
Biosensors & bioelectronics 2020 v.164 pp. 112320
ISSN:
0956-5663
Subject:
bovine serum albumin; human serum albumin,   etc   ; biomarkers; biosensors; detection limit; electrical resistance; graphene; graphene oxide; microRNA; neoplasms; oxidation;     Show all 11 Subjects
Abstract:
... In this study we developed a uniform, large-area, layered graphene composite of graphene oxide/graphene (GO/G) for the detection of circulating miRNA-21, a reliable biomarker for early cancer diagnosis. We prepared this layered composite of GO/G through low-damage plasma treatment of bilayer G. The top layer of G was oxidized (i.e., atomic layer oxidation) to form a GO layer, which acted as the bi ...
DOI:
10.1016/j.bios.2020.112320

9. Bovine Serum Albumin Nanoparticles for the Efficient Delivery of Berberine: Preparation, Characterization and In vitro biological studies

Author:
Solanki, Raghu; Patel, Krunal; Patel, Sunita
Source:
Colloids and surfaces 2021 v.608 pp. 125501
ISSN:
0927-7757
Subject:
bovine serum albumin,   etc   ; Fourier transform infrared spectroscopy; X-ray diffraction; antineoplastic activity; aqueous solutions; berberine; bioavailability; breast neoplasms; differential scanning calorimetry; drugs; encapsulation; ethidium; nanoparticles; neoplasm cells; pH; particle size; scanning electron microscopy; staining; therapeutics; thermogravimetry; toxicity; toxicity testing; water solubility;     Show all 23 Subjects
Abstract:
... Berberine (BBR) is a naturally occurring plant-derived isoquinoline alkaloid. It has been reported to exhibit multiple pharmacological properties including anticancer activity. However, its low water solubility and bioavailability restrict its clinical use for cancer treatment. Hence, to overcome these limitations and enhance its therapeutic efficacy, in the present study we have encapsulated BBR ...
DOI:
10.1016/j.colsurfa.2020.125501

10. Latest developments in the detection and separation of bovine serum albumin using molecularly imprinted polymers

Author:
Jahanban-Esfahlan, Ali; Roufegarinejad, Leila; Jahanban-Esfahlan, Rana; Tabibiazar, Mahnaz; Amarowicz, Ryszard
Source:
Talanta 2019
ISSN:
0039-9140
Subject:
bovine serum albumin; human serum albumin,   etc   ; molecular imprinting; polymers; prices;     Show all 5 Subjects
Abstract:
... Recently, enormous attention has been focused on the development of protein-molecularly imprinted polymers (MIPs). In this sense, bovine serum albumin (BSA) is well regarded as a favorite template in various MIPs-based biochemical/analytical assays mainly due to its low price, easy availability, and high structural homology to human serum albumin (HSA). Equally, the implications of BSA in the path ...
DOI:
10.1016/j.talanta.2019.120317

11. A novel approach to calculate protein adsorption isotherms by molecular dynamics simulations

Author:
Jakobtorweihen, Sven; Heuer, Jonas; Waluga, Thomas
Source:
Journal of chromatography 2020 v.1620 pp. 460940
ISSN:
0021-9673
Subject:
bovine serum albumin; human serum albumin,   etc   ; Gibbs free energy; adsorption; agarose; cattle; chromatography; hemoglobin; molecular dynamics; prediction; simulation models; sorption isotherms;     Show all 12 Subjects
Abstract:
... Protein adsorption plays a role in many fields, where in some it is desirable to maximize the amount adsorbed, in others it is important to avoid protein adsorption altogether. Therefore, theoretical methods are needed for a better understanding of the underlying processes and for the prediction of adsorption quantities. In this study, we present a proof-of-concept that the calculation of protein ...
DOI:
10.1016/j.chroma.2020.460940

12. Probing the interaction of Rivastigmine Tartrate, an important Alzheimer's drug, with serum albumin: Attempting treatment of Alzheimer's disease

Author:
Shamsi, Anas; Mohammad, Taj; Anwar, Saleha; Alajmi, Mohamed F.; Hussain, Afzal; Hassan, Md. Imtaiyaz; Ahmad, Faizan; Islam, Asimul
Source:
International journal of biological macromolecules 2020 v.148 pp. 533-542
ISSN:
0141-8130
Subject:
bovine serum albumin; human serum albumin,   etc   ; Alzheimer disease; blood plasma; calorimetry; circular dichroism spectroscopy; computer simulation; drugs; fluorescence emission spectroscopy; models; molecular dynamics; temperature; therapeutics; thermodynamics; titration;     Show all 15 Subjects
Abstract:
... The present study was aimed at investigating the binding between an important drug of Alzheimer's therapy, Rivastigmine tartrate (RT), with Bovine serum albumin (BSA). BSA is a model protein that is increasingly being used for studies related to drug-protein interaction owing to its structural similarity with human serum albumin (HSA) which is extremely abundant in the circulatory system comprisin ...
DOI:
10.1016/j.ijbiomac.2020.01.134

13. Organ-Specific Screening for Protein Damage Using Magnetic Bead Bioreactors and LC–MS/MS

Author:
Jiang, Di; Shen, Min; Ahiadu, Ben; Rusling, James F.
Source:
Analytical chemistry 2020 v.92 no.7 pp. 5337-5345
ISSN:
1520-6882
Subject:
bovine serum albumin; human serum albumin,   etc   ; acetaminophen; bioreactors; cytochrome P-450; glutathione transferase; humans; liquid chromatography; liver microsomes; magnetism; metabolites; models; peptides; screening; tandem mass spectrometry;     Show all 15 Subjects
Abstract:
... A new 96-well plate methodology for fast, enzyme-multiplexed screening for metabolite–protein adducts was developed. Magnetic beads coated with metabolic enzymes were used to make potentially reactive metabolites that can react with test protein in the wells, followed by sample workup in multiple 96-well filter plates for LC–MS/MS analysis. Incorporation of human microsomes from multiple organs an ...
DOI:
10.1021/acs.analchem.9b05871

14. Self-Driven BSA Surface Imprinted Magnetic Tubular Carbon Nanofibers: Fabrication and Adsorption Performance

Author:
Yang, Zuoting; Chen, Junjie; Wang, Jiqi; Zhang, Qiuyu; Zhang, Baoliang
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.8 pp. 3241-3252
ISSN:
2168-0485
Subject:
bovine serum albumin; human serum albumin,   etc   ; adsorption; carbon nanofibers; cytochrome c; dopamine; energy conservation; fetal bovine serum; iron; lysozyme; magnetic properties; magnetism; molecular imprinting; nanoparticles; ovalbumin; sustainable development;     Show all 16 Subjects
Abstract:
... The development of materials with high adsorption capacity and selectivity, simple operation, and short adsorption equilibrium time are still a focus in the domain of imprinting techniques. Based on this, a novel water-compatible imprinted material (SIPMTF) on the surface of magnetic carriers is prepared by one-pot method. Surface imprinted magnetic tubular carbon nanofibers (SIPMTFs) are based on ...
DOI:
10.1021/acssuschemeng.9b06829

15. Eco-friendly solvents and their mixture for the fabrication of polysulfone ultrafiltration membranes: An investigation of doctor blade and slot die casting methods

Author:
Dong, Xiaobo; Jeong, Tae J.; Kline, Eric; Banks, Lillian; Grulke, Eric; Harris, Tequila; Escobar, Isabel C.
Source:
Journal of membrane science 2020 v.614 pp. 118510
ISSN:
0376-7388
Subject:
bovine serum albumin,   etc   ; artificial membranes; chemical structure; evaporation; extrusion; lactones; permeability; roughness; separation; solvents; toxicity; ultrafiltration;     Show all 12 Subjects
Abstract:
... Nonsolvent induced phase separation has been widely used to fabricate polymeric membranes. Common solvents, such as dimethylacetamide (DMAc) and N-Methyl-2-pyrrolidone (NMP), are toxic and not environmentally friendly; therefore, eco-friendly alternatives are needed. Eco-friendly solvents, such as Rhodiasolv® PolarClean (PolarClean) and γ-valerolactone (GVL), have been investigated to replace DMAc ...
DOI:
10.1016/j.memsci.2020.118510

16. Biomacromolecular fluorescent nanoparticles co-assembled by bovine serum albumin and DNA segments for living cell imaging

Author:
Sun, Si; Xiao, Qian-Ru; Ye, Cui-Fang; Jiang, Yong
Source:
Colloids and surfaces 2020 v.603 pp. 125255
ISSN:
0927-7757
Subject:
bovine serum albumin,   etc   ; DNA; bioimaging; cost effectiveness; cytoplasm; engineering; fluorescence; human cell lines; nanoparticles; Michigan;     Show all 10 Subjects
Abstract:
... Fluorescent nanoparticles have been widely used in the field of biomedical engineering because they are usually biocompatible, cost-effective and homogeneously uniform. In this study, we describe a biomacromolecular fluorescent nanoparticle prepared with a facile disolvating process using bovine serum albumin, DNA segments and SYBR Green I. The obtained co-assemblies, termed DNA@BSA nanoparticles, ...
DOI:
10.1016/j.colsurfa.2020.125255

17. Ultraefficient stabilization of high internal phase emulsions by globular proteins in the presence of polyols: Importance of a core-shell nanostructure

Author:
Xu, Y.T.; Tang, C.H.; Binks, B.P.
Source:
Food hydrocolloids 2020 v.107 pp. 105968
ISSN:
0268-005X
Subject:
bovine serum albumin,   etc   ; cosmetics; droplets; emulsions; gels; heat; hydrocolloids; models; nanomaterials; oils; polyols; storage time; trehalose;     Show all 13 Subjects
Abstract:
... We report that oil-in-water (o/w) high internal phase emulsions (HIPEs) can be ultra-efficiently stabilized by globular proteins in the presence of appropriate concentrations of polyols. Using bovine serum albumin (BSA) and trehalose as the model globular protein and polyol, stable HIPE gels at 0.8 oil volume fraction can be easily prepared at a protein concentration in the aqueous phase as low as ...
DOI:
10.1016/j.foodhyd.2020.105968

18. Studies on interactions of pentagalloyl glucose, ellagic acid and gallic acid with bovine serum albumin: A spectroscopic analysis

Author:
Zhang, Liangliang; Liu, Yuchen; Hu, Xinyu; Xu, Man; Wang, Yongmei
Source:
Food chemistry 2020 v.324 pp. 126872
ISSN:
0308-8146
Subject:
bovine serum albumin,   etc   ; circular dichroism spectroscopy; ellagic acid; fluorescence; gallic acid; glucose; pH; spectral analysis;     Show all 8 Subjects
Abstract:
... Fluorescence techniques and circular dichroism (CD) were utilized to investigate the interactions of a typical gallotannin 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) and two simple phenolic compounds ellagic acid (EA) and gallic acid (GA) with bovine serum albumin (BSA). Fluorescence experiments showed that PGG and EA could strongly interact with BSA. The binding constants showed a pH-depen ...
DOI:
10.1016/j.foodchem.2020.126872

19. Selenium in Se-enriched tartary buckwheat (Fagopyrum tataricum L. Gaertn.): Its molecular form and changes during processing

Author:
Niu, Qianwen; Dong, Rui; Messia, Maria Cristina; Ren, Tian; Hu, Xinzhong
Source:
Journal of cereal science 2020 v.95 pp. 103022
ISSN:
0733-5210
Subject:
albumins,   etc   ; Fagopyrum tataricum; buckwheat; flavonoids; flour; glutelins; nutrients; processing technology; pseudocereals; selenium; selenomethionine; China;     Show all 12 Subjects
Abstract:
... The essential trace mineral, selenium (Se), is of fundamental importance to human health. Tartary buckwheat is a pseudocereal variety rich in functional flavonoids. To investigate the characters of Se-enriched Tartary buckwheat in Shaanxi province, we studied effect of different processing methods on its nutrients and selenium distribution in Se-enriched buckwheat groat (Se-BG), milled flour (Se-B ...
DOI:
10.1016/j.jcs.2020.103022

20. High flux mixed matrix membrane with low albumin leakage for blood plasma detoxification

Author:
Kim, DooLi; Stamatialis, Dimitrios
Source:
Journal of membrane science 2020 v.609 pp. 118187
ISSN:
0376-7388
Subject:
albumins,   etc   ; adsorption; artificial membranes; blood plasma; filtration; hemodialysis; humans; mortality; patients; protein content; sorbents; toxins;     Show all 12 Subjects
Abstract:
... Current hemodialysis (HD) therapy removes well small sized toxins but removes less effectively middle molecules and protein-bound uremic toxins (PBUTs). This limited removal has been associated to high mortality of patients due to increased cardiovascular events. The hemodiafiltration therapy, which combines diffusive and convective transport using high flux membranes, can achieve higher removal o ...
DOI:
10.1016/j.memsci.2020.118187