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1. A label-free shotgun proteomics analysis of macadamia nut

Author:
Rost, Johanna; Muralidharan, Sridevi; Lee, N. Alice
Source:
Food research international 2020 v.129 pp. 108838
ISSN:
0963-9969
Subject:
amino acid metabolism,   etc   ; allergens; antimicrobial peptides; lipid metabolism; macadamia nuts; proteomics; stress response; vicilin;     Show all 8 Subjects
Abstract:
... In this study, we present a systematic proteomic overview of macadamia nut using a label-free shotgun proteomic approach. We identified 947 proteins in 723 clusters and gene ontology analysis revealed proteins across 46 functional categories including carbohydrate metabolism (10%), protein metabolic processes (5%), amino acid metabolism (4%), transport (4%), stress response (3%), lipid metabolism ...
DOI:
10.1016/j.foodres.2019.108838

2. Multitasking Actors of Staphylococcus aureus Metabolism and Virulence

Author:
Tan, Xin; Coureuil, Mathieu; Charbit, Alain; Jamet, Anne
Source:
Trends in microbiology 2020 v.28 no.1 pp. 6-9
ISSN:
0966-842X
Subject:
amino acid metabolism,   etc   ; Staphylococcus aureus; biochemical pathways; carbohydrates; lipids; nucleic acids; pathogenesis; virulence;     Show all 8 Subjects
Abstract:
... Recent studies have uncovered the striking commonality of multitasking molecular actors linking metabolism and virulence regulation. Beyond the well known importance of carbohydrate and amino acid metabolism regulators in coordinating metabolic pathways and pathogenesis, we highlight recent major advances linking lipid and nucleic acid pathways to virulence regulation in Staphylococcus aureus. ...
DOI:
10.1016/j.tim.2019.10.013

3. Correction to: Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells

Author:
Kieliszek, Marek; Błażejak, Stanisław; Bzducha-Wróbel, Anna; Kot, Anna M.
Source:
Biological trace element research 2019 v.187 no.1 pp. 328
ISSN:
0163-4984
Subject:
amino acid metabolism,   etc   ; lipids; selenium; yeasts;     Show all 4 Subjects
Abstract:
... The authors forgot to include the following information in Materials and Methods. ...
DOI:
10.1007/s12011-018-1390-2
PubMed:
29858965
PubMed Central:
PMC6828426

4. Genome analysis of Crassaminicella sp. SY095, an anaerobic mesophilic marine bacterium isolated from a deep-sea hydrothermal vent on the Southwest Indian Ridge

Author:
Li, Xue-Gong; Zhang, Wei-Jia; Qi, Xiao-Qing; Wu, Long-Fei
Source:
Marine genomics 2020 v.52 pp. 100733
ISSN:
1874-7787
Subject:
amino acid metabolism,   etc   ; aquatic bacteria; chromosomes; genes; nucleotide sequences; peptides; plasmids; ribosomal RNA; sequence analysis; sporulation; transfer RNA; virion;     Show all 12 Subjects
Abstract:
... Crassaminicella sp. strain SY095 is an anaerobic mesophilic marine bacterium that was recently isolated from a deep-sea hydrothermal vent on the Southwest Indian Ridge. Here, we present the complete genome sequence of strain SY095. The genome consists of a chromosome of 3,046,753 bp (G + C content of 30.81%) and a plasmid of 36,627 bp (G + C content of 31.29%), encodes 2966 protein, 135 tRNA genes ...
DOI:
10.1016/j.margen.2019.100733

5. Effects of exposure to polyether sulfone microplastic on the nitrifying process and microbial community structure in aerobic granular sludge

Author:
Qin, Ronghua; Su, Chengyuan; Liu, Weihong; Tang, Linqin; Li, Xinjuan; Deng, Xue; Wang, Anliu; Chen, Zhengpeng
Source:
Bioresource technology 2020 v.302 pp. 122827
ISSN:
0960-8524
Subject:
amino acid metabolism,   etc   ; ammonium nitrogen; community structure; high-throughput nucleotide sequencing; microbial communities; microplastics; nitrate reduction; nitrites; sludge; total nitrogen;     Show all 10 Subjects
Abstract:
... The effects of polyether sulfone (PES) microplastic concentration on the nitrifying process of aerobic granular sludge (AGS) were investigated together with the microbial community structure of AGS. The PES microplastic concentration inhibited the removal of ammonia nitrogen only to a small extent. The average total nitrogen removal rate increased by 5.6% after PES addition. On the 30th day, the a ...
DOI:
10.1016/j.biortech.2020.122827

6. Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

Author:
Wan, Liyun; Lei, Yong; Yan, Liying; Liu, Yue; Pandey, Manish K.; Wan, Xia; Varshney, Rajeev K.; Fang, Jiahai; Liao, Boshou
Source:
BMC plant biology 2020 v.20 no.1 pp. 161
ISSN:
1471-2229
Subject:
amino acid metabolism,   etc   ; biosynthesis; color; flavonoids; genetic analysis; glycolysis; metabolome; multiomics; mutants; mutation; peanut oil; peanuts; phenotype; pigmentation; plant hormones; suberin; testa; transcriptome; tricarboxylic acid cycle;     Show all 19 Subjects
Abstract:
... BACKGROUND: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation. RESULT: Metabolomes revealed flavono ...
DOI:
10.1186/s12870-020-02383-7
PubMed:
32293272
PubMed Central:
PMC7161308

7. Metabolomics study on revealing the inhibition and metabolic dysregulation in Pseudomonas fluorescens induced by 3-carene

Author:
Shu, Huizhen; Zhang, Weimin; Yun, Yonghuan; Chen, Weijun; Zhong, Qiuping; Hu, Yueying; Chen, Haiming; Chen, Wenxue
Source:
Food chemistry 2020 v.329 pp. 127220
ISSN:
0308-8146
Subject:
amino acid metabolism,   etc   ; Pseudomonas fluorescens; biosynthesis; carene; coenzyme A; food contamination; mass spectrometry; metabolites; metabolomics; multivariate analysis; tricarboxylic acid cycle; ultra-performance liquid chromatography;     Show all 12 Subjects
Abstract:
... 3-Carene is a monoterpenoid that has an effective inhibitory ability against Pseudomonas fluorescens (P. fluorescens) which can induce a range of food contamination problems. In this study, ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)-based metabolomics was used to elucidate the antimicrobial mechanism of 3-carene in P. fluorescens. Multivariate analysis of the metabolite da ...
DOI:
10.1016/j.foodchem.2020.127220

8. Dietary Luffa cylindrica (L.) Roem promotes branched-chain amino acid catabolism in the circulation system via gut microbiota in diet-induced obese mice

Author:
Zhang, Lu; Yue, Yunshuang; Shi, Mengxuan; Tian, Meiling; Ji, Junfu; Liao, Xiaojun; Hu, Xiaosong; Chen, Fang
Source:
Food chemistry 2020 v.320 pp. 126648
ISSN:
0308-8146
Subject:
amino acid metabolism,   etc   ; Luffa aegyptiaca; amino acids; animal disease models; biosynthesis; body mass index; digestive system; enzymes; intestinal microorganisms; mice; nutritional intervention; obesity; prediction; vegetables;     Show all 14 Subjects
Abstract:
... High circulating branched-chain amino acid (BCAA) levels can be diagnosis indicators for obesity. Luffa cylindrica (luffa) is one of vegetables against obesity. However, whether the anti-obesity of luffa is associated with BCAA metabolism and gut microbiota remains unknown. Here, we used conventionally raised diet-induced obese (DIO) mice to prove dietary luffa could reduce higher circulating BCAA ...
DOI:
10.1016/j.foodchem.2020.126648

9. α-ketobutyrate links alterations in cystine metabolism to glucose oxidation in mtDNA mutant cells

Author:
Gokhale, Amrita; Lesner, Nicholas P.; Gokhale, Amrita S.; Kota, Kalyani; DeBerardinis, Ralph J.; Mishra, Prashant
Source:
Metabolic engineering 2020 v.60 pp. 157-167
ISSN:
1096-7176
Subject:
amino acid metabolism,   etc   ; adenosine triphosphate; carbon; cystine; electron transport chain; glucose; glutamine; isotope labeling; metabolic flux analysis; mitochondria; mitochondrial DNA; mitochondrial genome; mutants; mutation; oxidation; stable isotopes;     Show all 16 Subjects
Abstract:
... Pathogenic mutations in the mitochondrial genome (mtDNA) impair organellar ATP production, requiring mutant cells to activate metabolic adaptations for survival. Understanding how metabolism adapts to clinically relevant mtDNA mutations may provide insight into cellular strategies for metabolic flexibility. In this study, we use ¹³C isotope tracing and metabolic flux analysis to investigate centra ...
DOI:
10.1016/j.ymben.2020.03.010

10. Metabolomics analysis of Actinosynnema pretiosum with improved AP-3 production by enhancing UDP-glucose biosynthesis

Author:
Liu, Ting; Jin, Ziwen; Wang, Ziwei; Chen, Jun; Wei, Liu-Jing; Hua, Qiang
Source:
Journal of bioscience and bioengineering 2020 v.130 no.1 pp. 36-47
ISSN:
1389-1723
Subject:
amino acid metabolism,   etc   ; Actinosynnema pretiosum; antineoplastic activity; biochemical pathways; biosynthesis; fatty acids; feeding methods; fructose; genetic engineering; metabolomics; mutants; pleiotropy; quantitative polymerase chain reaction;     Show all 13 Subjects
Abstract:
... Ansamitocin P-3 (AP-3) shows strong anticancer effects and has used as a payload for antibody–drug conjugates. Our previous study have shown that although genetically engineered Actinosynnema pretiosum strains with enhanced UDP-glucose (UDPG) biosynthesis displayed improved AP-3 production compared to the wild-type strain, the increase in yield was far from meeting the industrial demand. In this s ...
DOI:
10.1016/j.jbiosc.2020.02.013

11. Rice grains alleviate cadmium toxicity by expending glutamate and increasing manganese in the cadmium contaminated farmland

Author:
Yuan, Kai; Wang, Changrong; Zhang, Changbo; Huang, Yongchun; Wang, Peipei; Liu, Zhongqi
Source:
Environmental pollution 2020 v.262 pp. 114236
ISSN:
0269-7491
Subject:
amino acid metabolism,   etc   ; Oryza sativa; agricultural land; alanine; aleurone layer; cadmium; calcium; cations; cultivars; glutamic acid; iron; manganese; nutrients; phenylalanine; rice; soil; threonine; toxicity; zinc;     Show all 19 Subjects
Abstract:
... The accumulation of cadmium (Cd) in rice grains is closely associated with the content of mineral nutrients and amino acid metabolism, but the causal link among them is unclear. Profiles of amino acids (AAs) and quantities of essential nutrients in grains from early and late rice cultivars grown at four sites with different Cd levels were analyzed in the present study. Hazard quotients (HQs) for c ...
DOI:
10.1016/j.envpol.2020.114236

12. Assessment of folate and cobalamin concentrations in relation to their dependent intracellular metabolites in serum of pigs between 6 and 26 weeks of age

Author:
Grützner, Niels; Opriessnig, Tanja; Lopes, Rosana; Suchodolski, Jan S.; Nathues, Heiko; Steiner, Jörg M.
Source:
Research in veterinary science 2020 v.130 pp. 59-67
ISSN:
0034-5288
Subject:
amino acid metabolism,   etc   ; blood serum; folic acid; homocysteine; intracellular enzymes; metabolites; methionine synthase; methylmalonic acid; methylmalonyl-CoA mutase; moieties; nucleic acids; swine; vitamin B12;     Show all 13 Subjects
Abstract:
... Folate (vitamin B₉) and cobalamin (vitamin B₁₂) play an important role in amino acid metabolism, nucleic acid synthesis, and methyl group transfer. Two intracellular enzymes, methionine synthase and methylmalonyl-CoA mutase, are folate and/or cobalamin-dependent, respectively. At the cellular level, a lack of folate and cobalamin leads to accumulation of serum homocysteine (HCY) and a lack of coba ...
DOI:
10.1016/j.rvsc.2020.02.002

13. Metagenomic analysis revealed the succession of microbiota and metabolic function in corncob composting for preparation of cultivation medium for Pleurotus ostreatus

Author:
Kong, Weili; Sun, Bo; Zhang, Jianyun; Zhang, Yuting; Gu, Likun; Bao, Lijun; Liu, Shuaixia
Source:
Bioresource technology 2020 v.306 pp. 123156
ISSN:
0960-8524
Subject:
amino acid metabolism,   etc   ; Firmicutes; Pleurotus ostreatus; Proteobacteria; biochemical pathways; carbon nitrogen ratio; composting; composts; corn cobs; galactose; lipids; metabolomics; metagenomics; microorganisms; pH; starch; sucrose; temperature;     Show all 18 Subjects
Abstract:
... Metagenomic sequencing was used to reveal the dynamic changes in microbiota and the metabolic functions in corncob composting for preparing cultivation medium of Pleurotus ostreatus. Results showed that the changes of physicochemical properties lead to different dominant phylum at different stages of composting. Firmicutes replaced Proteobacteria as the dominant phylum at thermophilic stage. Corre ...
DOI:
10.1016/j.biortech.2020.123156

14. Phytotoxicity and metabolic responses induced by tetrachlorobiphenyl and its hydroxylated and methoxylated derivatives in rice (Oryza sative L.)

Author:
Lin, Fangjing; Sun, Jianteng; Liu, Na; Zhu, Lizhong
Source:
Environment international 2020 v.139 pp. 105695
ISSN:
0160-4120
Subject:
amino acid metabolism,   etc   ; Oryza; biosynthesis; biotransformation; crops; energy; environmental assessment; fatty acids; growth retardation; hydroxylation; metabolomics; mitochondria; moieties; phytotoxicity; polychlorinated biphenyls; pyruvic acid; rice; seedlings; tricarboxylic acid cycle;     Show all 19 Subjects
Abstract:
... Polychlorinated biphenyls (PCBs) and their biotransformation products, hydroxylated (OH-PCBs) and methoxylated derivatives (MeO-PCBs), have been detected in the environment and biota, especially crops. However, to date, little information is available on the phytotoxicity and metabolic responses induced by these chemicals in crops. In this study, we exposed rice (Oryza sative L.) seedlings to 2,3, ...
DOI:
10.1016/j.envint.2020.105695

15. Single and combined effects of the “Deadly trio” hypoxia, hypercapnia and warming on the cellular metabolism of the great scallop Pecten maximus

Author:
Götze, Sandra; Bock, Christian; Eymann, Charlotte; Lannig, Gisela; Steffen, Jennifer B.M.; Pörtner, Hans-O.
Source:
Comparative biochemistry and physiology 2020 v.243-244 pp. 110438
ISSN:
1096-4959
Subject:
amino acid metabolism,   etc   ; Pecten maximus; acute effects; aquatic organisms; biochemical pathways; carbon dioxide; climatic factors; energy metabolism; hypercapnia; hypoxia; mitochondria; oxygen; scallops; temperature;     Show all 14 Subjects
Abstract:
... In the ocean the main climate drivers affecting marine organisms are warming, hypercapnia, and hypoxia. We investigated the acute effects of warming (W), warming plus hypercapnia (WHc, ~1800 μatm CO₂), warming plus hypoxia (WHo, ~12.1 kPa O₂), and a combined exposure of all three drivers (Deadly Trio, DT) on king scallops (Pecten maximus). All exposures started at 14 °C and temperature was increas ...
DOI:
10.1016/j.cbpb.2020.110438

16. Glutamicibacter mishrai sp. nov., isolated from the coral Favia veroni from Andaman Sea

Author:
Das, Lipika; Deb, Sushanta; Das, Subrata K.
Source:
Archives of microbiology 2020 v.202 no.4 pp. 733-745
ISSN:
0302-8933
Subject:
amino acid metabolism,   etc   ; Actinobacteria; DNA; ancestry; biogenesis; cell walls; chemotaxonomy; corals; genes; genomics; glycolipids; new species; peptidoglycans; phenotype; phospholipids; quinones; ribosomal RNA; Andaman Sea; India;     Show all 19 Subjects
Abstract:
... A novel aerobic marine actinobacterium (strain S5–52ᵀ) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India. Cells are Gram stain positive and rod shaped. The DNA G+C content was 58.7 mol%. The major quinones were MK-8 and MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid, trimannosyldiacylglycerol, p ...
DOI:
10.1007/s00203-019-01783-0

17. Haloplanus rubicundus sp. nov., an extremely halophilic archaeon isolated from solar salt

Author:
Kim, Yeon Bee; Kim, Joon Yong; Song, Hye Seon; Lee, Se Hee; Shin, Na-Ri; Bae, Jin-Woo; Myoung, Jinjong; Lee, Ki-Eun; Cha, In-Tae; Rhee, Jin-Kyu; Roh, Seong Woon
Source:
Systematic and applied microbiology 2020 v.43 no.3 pp. 126085
ISSN:
0723-2020
Subject:
amino acid metabolism,   etc   ; Archaea; amino acids; biogenesis; carbohydrate metabolism; chemotaxonomy; computer simulation; genome; genomics; glycolipids; new species; nucleic acid hybridization; phenotype; phosphates; phylogeny; sulfates; Korean Peninsula;     Show all 17 Subjects
Abstract:
... Two extremely halophilic archaea strains, CBA1112ᵀ and CBA1113, were isolated from solar salt in Korea. The genome sizes and G+C content of CBA1112ᵀ and CBA1113 were 3.77 and 3.53Mb, and 66.0 and 66.5mol%, respectively. Phylogenetic analysis based on closely related taxa and environmental Haloplanus sequences indicated that both CBA1112ᵀ and CBA1113 strains are grouped within the genus Haloplanus. ...
DOI:
10.1016/j.syapm.2020.126085

18. Neonicotinoid insecticides exposure cause amino acid metabolism disorders, lipid accumulation and oxidative stress in ICR mice

Author:
Yan, Sen; Meng, Zhiyuan; Tian, Sinuo; Teng, Miaomiao; Yan, Jin; Jia, Ming; Li, Ruisheng; Zhou, Zhiqiang; Zhu, Wentao
Source:
Chemosphere 2020 v.246 pp. 125661
ISSN:
0045-6535
Subject:
amino acid metabolism,   etc   ; acetamiprid; branched chain amino acids; dinotefuran; free fatty acids; lethal dose 50; lipids; metabolomics; mice; nitenpyram; oxidative stress; pest control; phenylalanine; risk; toxicology;     Show all 15 Subjects
Abstract:
... Neonicotinoids are increasingly being used for pest control, and their potential health risks are now receiving attention. In this study, the toxic effects of three neonicotinoids (dinotefuran, nitenpyram and acetamiprid) were evaluated in ICR mice. After 30 days of exposure to neonicotinoids (1/200 LD50), oxidative stress levels, biochemical parameters, free fatty acids contents, and ¹H NMR-based ...
DOI:
10.1016/j.chemosphere.2019.125661

19. Shotgun metagenomics approach reveals the bacterial community and metabolic pathways in commercial hongeo product, a traditional Korean fermented skate product

Author:
Zhao, Chang-Cheng; Eun, Jong-Bang
Source:
Food research international 2020 v.131 pp. 109030
ISSN:
0963-9969
Subject:
amino acid metabolism,   etc   ; Clostridium; Marinilactibacillus; Pseudomonas; Psychrobacter; ammonia; bacterial communities; biochemical pathways; carbohydrate metabolism; fish processing plants; flavor; genes; lactic acid bacteria; metagenomics; nitrogen;     Show all 15 Subjects
Abstract:
... The aim of this study was to investigate the microbial diversity and microbial metabolic pathways using a metagenomic approach in commercial hongeo samples collected from five different fish processing plants. Community comparison analysis indicated that hongeo samples from different fish processing plants have a similar microbial structure at genus level, but the relative abundance of these gener ...
DOI:
10.1016/j.foodres.2020.109030

20. Effects of prenatal cold stress on maternal serum metabolomics in rats

Author:
Lian, Shuai; Li, Wenjie; Wang, Di; Xu, Bin; Guo, Xueheng; Yang, Huanmin; Wang, Jianfa
Source:
Life sciences 2020 v.246 pp. 117432
ISSN:
0024-3205
Subject:
amino acid metabolism,   etc   ; arachidonic acid; blood serum; cold stress; glutamic acid; glutamine; immune system; inflammation; linoleic acid; mass spectrometry; metabolites; metabolomics; neurosecretory system; progeny;     Show all 14 Subjects
Abstract:
... Our previous studies have shown that prenatal cold stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal cold stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by w ...
DOI:
10.1016/j.lfs.2020.117432